PLK4 as a Key Regulator of Neuroblastoma Differentiation and a Promising Therapeutic Target.

Neuroblastoma (NB) differentiation status critically influences prognosis and treatment response. Although differentiation therapy has shown clinical benefit, its efficacy remains limited. The molecular mechanisms driving NB differentiation are not fully understood.

AlO/MgO-doped, CaO-based adsorbents for CO capture: A performance study.

We investigated direct calcination of four precursors: calcium oxalate (CaCO; denoted as CaO-1), calcium carbonate (CaCO; CaO-2), calcium d-gluconate monohydrate (CHCaO·HO; CaO-3), and a commercial calcium carbonate (CaO-4). The effects of precursor selection on CO adsorption performance were systematically compared. CaO-1 exhibited superior initial CO adsorption capacity (0.

Lactylation in cancer biology: Unlocking new avenues for research and therapy.

Lactylation, a newly identified post-translational modification, plays a multifaceted role in cancer biology by integrating epigenetic and non-epigenetic mechanisms. This review summarizes the latest research progress on lactylation, including its functions in epigenetic regulation and its broader impact on cellular processes. Lactate, as a metabolic byproduct, not only serves as an energy source for tumor cells but also acts as a signaling molecule driving various oncogenic processes.

Optimizing CaO-based CO adsorption: Impact of operating parameters.

CaO-based adsorbents present a promising approach for industrial CO emissions reduction. This study systematically investigates the influence of adsorption temperature, adsorbent mass, CO concentration, and flow rate on CO capture efficiency using commercial CaO via thermogravimetric analysis (TGA). Optimal adsorption performance, achieving a capacity of 0.

Cognitive impairment in cerebellar phenotype of multiple system atrophy and its association with hot-cross bun sign.

Background: Cognitive impairment has been reported in patients with cerebellar phenotype of multiple system atrophy (MSA-C), yet this area remains largely unexplored.

Objective: We evaluated the cognitive profile of MSA-C patients and assessed its association with neuroimaging features, specifically the hot-cross bun (HCB) sign.

Methods: This retrospective study encompassed 210 patients diagnosed with MSA-C between 2019 and 2023.

Multi-Omics Analysis Reveals the transforming growth factor-β Signaling-Driven Multicellular Interactions with Prognostic Relevance in Cervical Cancer Progression.

While cervical cancer (CC) prognosis depends on tumor staging, the spatiotemporal evolution of tumor microenvironment (TME) heterogeneity during metastatic progression remains poorly characterized at single-cell resolution. We employed an integrative multi-omics approach, combining single-cell RNA sequencing (scRNA-seq; n = 11), spatial transcriptomics (ST), and bulk RNA-seq data from the TCGA-CESC cohort (n = 304), to systematically map TME remodeling across CC progression stages. scRNA-seq was performed on primary lesions from patients with localized (n = 3), regional (n = 4), and metastatic (n = 4) diseases, with in-depth analyses focusing on cellular characteristics, cell type composition alterations, functional changes, differentiation trajectories, and cell-cell interaction networks.

Inhibition of Wnt/β-catenin increases anti-tumor activity by synergizing with sorafenib in hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) poses a major global health challenge owing to limited treatment efficacy and drug resistance to therapies such as the tyrosine kinase inhibitor (TKI) sorafenib. We utilized a microfluidic three-dimensional (3D) drug testing system to assess drug responses in 37 fresh clinical samples and performed immunohistochemical analysis of 41 tumor tissue samples that received sorafenib therapy. Results revealed that Wnt/β-catenin activation was associated with sorafenib resistance, with higher nuclear β-catenin levels predicting poor response.

Achieving scalable expansion of therapeutic porcine hepatocytes in vivo through serial transplantation.

The clinical application of hepatocyte transplantation has been significantly hindered by the scarcity of primary hepatocytes and the functional immaturity of in vitro-produced hepatocytes. By performing serial allogeneic hepatocyte transplantation in CRISPR/Cas9-mediated Fah-knockout pigs, we successfully achieved large-scale expansion of hepatocytes while maintaining their authentic biological characteristics. Particularly, the established model enables sustained in vivo liver reconstruction, concurrently ameliorating hepatic fibrosis and demonstrating functional microenvironmental remodeling.

[Retracted] Reduced expression of Snail decreases breast cancer cell motility by downregulating the expression and inhibiting the activity of RhoA GTPase.

[This retracts the article DOI: 10.3892/ol.2013.

Carbon dots assemblies with zinc as activable fluorescence probe for local glucose monitoring in traumatic wounds.

Hyperglycemic microenvironment is a major cause of impaired wounds healing in diabetes. Real-time and rapid monitoring of the local glucose is essential for effective caring and intervening in diabetic wounds. Herein, an activatable fluorescence nanoprobe (Zn-CDs) was developed for rapid response to glucose.

Harnessing deep-sea cold seep microbiomes for reductive dehalogenation: from culturomics and genomics insights.

Deep-sea cold seeps harbor a rich and diverse repertoire of reductive dehalogenase-encoding genes (rdhA), yet their potential for reductive dehalogenation remains largely unexplored. In this study, we investigated the microbial debromination of 2,4,6-tribromophenol (TBP) in cold seep sediment microcosms. By optimizing culture conditions with different nutrient sources and substrate concentrations, we established a highly efficient debrominating microbial consortium capable of completely degrading 50 μM TBP within 72 h.

Carbon-Dot-Zn Assembly Hybrid Multifunctional Hydrogels for Synergistically Monitoring Local Glucose and Repairing Diabetic Wound.

The prolonged healing period and increased rates of amputation and mortality are significant challenges for diabetic patients' wound care in clinical practice. Therefore, there is an urgent need to develop timely and efficient wound management strategies to monitor the local glucose while promoting rapid wound healing. In this study, a multifunctional hydrogel (PF127@Zn/C-G) was constructed by coloading the monitoring and therapeutic carbon dot (CD)-based agents (Zn/C) and glucose oxidase (GOx).

Risk of ovarian cancer in women with pelvic inflammatory disease and homologous recombination repair gene mutations under 55: a population-based cohort study.

Objective: To address the relation among pelvic inflammatory disease (PID), genetic vulnerability and ovarian cancer (OC) risk, we assessed the association between PID and OC risk, alongside the interplay with germline homologous recombination repair (gHR) mutation, utilizing the UK Biobank.

Methods: We conducted a prospective cohort study in the UK Biobank by tracking OC incidences between individuals with and without a PID history. Identification of gHR mutations (, , , , ) carriers were accomplished through paired whole-exome sequencing data.

The intratumoral microbiota heterogenicity is related to the prognosis and tumorigenesis of cervical cancer.

Background: The intratumoral microbe-host interaction plays crucial role in the development of cancer. The microbiome can influence cancer development by modulating inflammation, immune responses and metabolic pathways. Therefore, we aim to delineate the landscape and role of intratumoral microbiota in cervical cancer (CC).

Long-term engraftment of human stem and progenitor cells for large-scale production of functional immune cells in engineered pigs.

Existing immunodeficient pig models have demonstrated limited success in supporting robust human haematopoietic engraftment and multilineage differentiation. Here we hypothesize that the targeted deletion of integrin-associated protein (Cd47) in severe combined immunodeficient pigs, with deletions in the X-linked interleukin-2 receptor gamma chain and recombination activating gene 1, would enable long-term haematopoietic engraftment following transplantation with human haematopoietic stem/progenitor cells. In Cd47-deficient pigs, we observed high levels of human haematopoietic chimerism in the blood and spleen, with functional T and B lymphocytes, natural killer and myeloid cells, as well as robust thymopoiesis.

Hematological and Biochemical Parameters of Subadult Captive Siberian Tigers ().

The conservation of Siberian tigers, the apex predators in the Asia-Pacific region, hinges on a thorough understanding of their physiological statuses and adaptive mechanisms. In order to fill a critical gap in our knowledge of this endangered species, we conducted comprehensive analyses of Siberian tiger whole blood and serum using modern hematology and biochemical analyzers. The blood samples were collected from subadult (2-3 years old) Siberian tigers ( = 15 for hematological analysis and = 7 for biochemical analysis).

A self-assembled fusion hydrophobin-coated nanodiamond system for synergistic targeted therapy of colorectal cancer.

A novel nano-platform (GE11-HGFI@ND-DOX) capable of specifically targeting colorectal cancer HCT-116 cells with highly expressed EGFR receptors, exhibiting precise targeting and enhanced therapy, while enhancing its dispersivity and hydrophilicity is reported. This work opens up prospects for the development of synergistic targeted therapy by integrating hydrophobins and nanodiamond encapsulated drug delivery systems.

Testicular tissue cryopreservation and transplantation as a strategy for feline conservation: a review of research advances.

As we humans continue our detrimental activities on the planet, the biodiversity loss is now seen as a big threat to entire ecosystem in which we all live. This issue becomes even more critical as we see a rapid increase in the number of animal species being listed as endangered, and a far greater rate of species extinction. We all know that felines play a crucial part in our ecosystems, it is therefore safe to argue that their conservation could play an important role in minimizing the biodiversity loss.

The low-dose CHK1 inhibitor prexasertib triggers VDAC1 dephosphorylation to activate mtDNA-STING signaling and synergize immunotherapy.

CHK1 inhibitors exhibit dose-limiting toxicity despite potent tumor cytotoxicity in clinical trials. Here, we reveal that low-dose prexasertib induces mtDNA damage by impairing repair machinery, triggering cytosolic mtDNA release via VDAC1 to activate STING-mediated innate immunity. Mechanistically, prexasertib blocks CHK1 phosphorylation and competitively recruits Nek1 kinase, thereby activating the ATR/CHK1 signaling cascade.

Rapid and sensitive identification of in blood based on M1 beads enrichment combined with multiple recombinase-aided PCR: a culture-independent approach.

Introduction: Clinically, timely diagnosis and effective treatment of bloodstream infections rely on rapid and sensitive detection methods. However, the long turn-around time and low detection rate of blood culture (the gold standard) make rapid diagnosis of challenging. This study develops a novel molecular assay (M1-mRAP) designed for the rapid and sensitive detection of three species in blood samples: (CA), (CT), and (CG).

A duplex one-step recombinase aided PCR assay for the rapid and sensitive detection of the isoniazid resistance genes katG and inhA in .

Objectives: Drug resistance in tuberculosis seriously affects the eradication of tuberculosis, and isoniazid resistance is the second most commonly observed drug resistance in patients with tuberculosis. Timely and accurate detection of isoniazid resistance is critical to the treatment of tuberculosis.

Methods: A duplex one-step recombinase-aided PCR (DO-RAP) assay was developed for the rapid and sensitive detection of the katG Ser315Thr and inhA-15 (C-T) mutations in , which are the most common isoniazid-resistant mutations.

17α-Methyltestosterone Affected Growth, Gonadal Development, and Intestinal Microbial Analysis in the Giant Freshwater Prawn ().

17α-methyltestosterone (MT) is known for its ability to suppress ovaries and induce spermatogenesis; yet, its effects in crustaceans are underexplored. This study investigates the impact of varying MT concentrations on the gonadal development and intestinal microbiota of juvenile . Feeds containing different MT doses were provided, and the sex ratios, histological observations, reproductive gene expression, and intestinal microbial composition were analyzed.

Systematic high-throughput evaluation reveals FrCas9's superior specificity and efficiency for therapeutic genome editing.

CRISPR-Cas9 systems have revolutionized genome editing, but the off-target effects of Cas9 limit its use in clinical applications. Here, we systematically evaluate FrCas9, a variant from , for cell and gene therapy (CGT) applications and compare its performance to SpCas9 and OpenCRISPR-1. OpenCRISPR-1 is a CRISPR system synthesized de novo using large language models (LLMs) but has not yet undergone systematic characterization.

Dual one-step recombinase-aided PCR for rapid detection of Candida in blood.

This study presents a novel dual one-step recombinase-aided PCR (DO-RAP) method, combined with recombinant human mannan-binding lectin protein (rhMBL; i.e., M1 protein)-conjugated magnetic bead (M1 bead) enrichment, for the early detection of Candida krusei and Candida parapsilosis bloodstream infections.