Multifunctional Roles and Therapeutic Prospects of C1q/TNF-Related Protein 3 (CTRP3) in Human Diseases.

Adipokines, including C1q/tumor necrosis factor (TNF)-related proteins (CTRPs), adiponectin, TNF-α, and leptin, are crucial bioactive molecules that are secreted by adipose tissue and circulate in the bloodstream. To date, 15 members of the CTRP family, which are collectively classified as part of the C1q/TNF superfamily, have been identified. Among these, CTRP3 stands out as a unique adipokine because of its distinct structural and functional properties.

Enhanced One-Pot Cas12a-Based Nucleic Acid Detection via Epitope Insertion and Recruitment of Rad51.

The CRISPR-Cas12a system has emerged as a promising tool for nucleic acid-based diagnostics. However, its multi-step workflow and limited sensitivity hinder its integration into point-of-care testing (POCT). Here, the ECOT system (Engineered Cas12a for One-pot Test), a novel approach that combines protein engineering with one-pot detection, offering high sensitivity, specificity, and rapid response is introduced.

The tumor suppressor DACT3 sensitizes triple-negative breast cancer to apatinib by inhibiting the Wnt/β-catenin pathway.

Apatinib, a small-molecule inhibitor of vascular endothelial growth factor receptor 2 (VEGFR2), shows efficient antitumor activity in heavily pretreated metastatic triple-negative breast cancer (TNBC). However, not all patients respond to apatinib, indicating that it is necessary to identify response biomarkers for more precise treatment and investigate the underlying mechanisms of apatinib resistance to develop new treatment strategies for TNBC. In this study, we identified the disheveled binding antagonist of beta-catenin 3 (DACT3) as a biomarker for apatinib sensitivity, as its expression level is significantly higher in apatinib-sensitive patients and positively correlates with longer survival.

Running exercise reverses depression-induced bone marrow myeloid skewing and neuroinflammation via the lactate-GPR81 axis.

Exercise has emerged as a powerful non-pharmacological intervention for mental health, yet its precise mechanisms in alleviating depression remain incompletely understood. Here, using a chronic unpredictable stress (CUS) mouse model, we demonstrate that prolonged stress induces hematopoietic stem cell (HSC) activation, leading to myeloid-biased hematopoiesis and an overproduction of Ly6C monocytes, which contribute to systemic and hippocampal inflammation. Notably, treadmill running exercise effectively mitigated depressive and anxiety-like behaviors in CUS mice by suppressing stress-induced HSC activation, restoring hematopoietic balance, and reducing peripheral Ly6C monocyte infiltration into the brain.

Exercise improves aging-induced cardiac dysfunction and prolongs lifespan via Hmgcr.

Since the incidence of cardiovascular disease increases dramatically with age, it is crucial to understand the molecular mechanisms of heart damage in aging and how to reduce the damage caused by aging to the heart. 3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (HMGCR) is the rate-limiting step in cholesterol biosynthesis and catalyzes the conversion of HMG-CoA to mevalonate (MVA). Hmgcr not only affects cholesterol synthesis during the development process, but also regulates primordial germ cell migration and affects heart development.

Antibacterial composite films fabricated from curcumin-loaded lignin micro-nanoparticles and cellulose.

Degradable regenerated cellulose (RC) composite films have prospects to replace plastic packaging. However, limitations such as lack of functionality, poor mechanical properties, inherent color, and the absence of antibacterial activity restrict their practical application. Herein, butyric anhydride esterified alkali lignin (BAL) encapsulated curcumin micro-nanoparticles were synthesized to develop high-antibacterial composite films.

Correction: A supramolecular nanovehicle toward systematic, targeted cancer and tumor therapy.

[This corrects the article DOI: 10.1039/C5SC00994D.].

Mechanistic insights into electric field-driven specific ion effects on nanoplastics aggregation in heavy metal co-contaminated aquatic systems.

The concurrent pollution of nanoplastics and heavy metals poses emerging threats to relevant aquatic systems. To elucidate the interfacial mechanisms governing nanoplastics-heavy metal aggregation, specifically clarify the unreconized role of electric fields on these processes, systematic investigation of carboxyl-modified polystyrene nanoplastics (PNs) aggregation mediated by Pb²⁺, Cu²⁺, Cd²⁺ and Zn²⁺ were conducted. Quantitative analyses revealed distinct specific ion effects on PNs aggregation, as evidenced by critical coagulation concentrations (CCC: Pb (1.

LINC00461 promotes macrophage M1 polarization by inhibiting KLF4 transcription.

Background: Macrophage polarization is a complex process whereby macrophages differentiate into M1 (pro-inflammatory) or M2 (anti-inflammatory) phenotypes, mediating distinct and often opposing functions in immunity and tissue repair.

Methods: We analyzed LINC00461 expression in M1- and M2-polarized macrophages using qPCR. Functional assays (in vitro and in vivo) were performed to assess the effects of LINC00461 knockdown on cytokine secretion.

Mining genetic loci and candidate genes related to salt tolerance traits in soybean.

Soybean is an important crop worldwide that provides ~ 50% oil for humans. Salinity is a major abiotic stress that inhibits soybean growth and yield. Dissecting the genetic basis of salt tolerance is an effective way for soybean plants to combat salt-related yield losses.

Hatcheries' contamination: the molecular characterization and pathogenicity of Mycoplasma gallisepticum isolates in China.

Mycoplasma gallisepticum (M. gallisepticum) is a significant pathogenic microorganism contaminating poultry hatchery environments. During intensive hatching, M.

Antibacterial efficacy of a K5-specific Klebsiella phage in milk and investigation of phage resistance mechanisms.

Klebsiella pneumoniae is an important pathogen causing bovine mastitis and milk contamination, with severe consequences for the dairy industry. Due to increasing bacterial antibiotic resistance, phage therapy has regained attention; however, studies on the antibacterial properties of Klebsiella phages in milk are limited. We evaluated the antibacterial efficacy of the K5-specific phage in milk at 4°C and 38°C with different multiplicities of infection (MOI = 10, 1, and 0.

Millimeter-scale interactions of nitrogen and potassium on denitrification, anammox, and Feammox in soil fertilization zones.

Soil denitrification, anammox, and Feammox are key for nitrogen (N) removal in agriculture. Despite potassium (K) fertilizer enhancing N efficiency, their role in regulation of these processes is unclear. A soil column incubation with N isotope tracing was conducted to explore millimeter-scale interactions of N and K on these pathways in soil fertilization zones.

Structure-affinity driven repurposing of natural compounds and herbal prescriptions for ulcerative colitis and psoriasis.

Background: Psoriasis and ulcerative colitis (UC) are immune-mediated chronic inflammatory diseases that affect barrier organs and share significant overlap in both pathogenesis and treatment strategies. Although the advent of biologics has transformed therapeutic options, their high cost often limits long-term accessibility, underscoring the need for alternative approaches.

Purpose: Natural compounds and traditional medicine represent valuable sources for novel drug discovery and repurposing, but systematic strategies for repurposing traditional medicines remain underdeveloped.

Within-host resistance evolution of ST15 Klebsiella pneumoniae in an ICU immunosuppressed patient under antibiotic pressure of polymyxins, ceftazidime-avibactam, and meropenem.

Objectives: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a major pathogen in healthcare-associated infections, posing a severe and potentially fatal threat to critically ill patients. This study aims to investigate the evolution of antimicrobial resistance under the selective pressure from multiple antibiotics in immunocompromised patients in intensive care unit (ICU).

Methods: In this study, we report the complex dynamic evolution of resistance in ST15 CRKP in an immunocompromised critically ill patient, driven by adjustments in antibiotic regimens involving polymyxin, ceftazidime-avibactam (CZA), and meropenem.

Unexpected Damage on Metal Artifacts Triggered by the Hazardous Interfacial Interaction from Aging of Polymer Coatings.

Polymer coatings are currently being employed for preservation of metal artifacts. However, there is insufficient awareness that inevitable aging of polymer coatings is bound to damage metal artifacts due to the lack of a direct and sensitive methodology to study the aging behaviors of polymers coated on the artifacts. Herein, we have developed an in situ and three-dimensional strategy to visualize the early stage aging behaviors of polymers coated on metal artifacts by lighting carboxyl groups generated from polymer aging.

EFHD1 promotes breast cancer progression through estrogen signaling.

Background: EF-hand domain family member D1 (EFHD1) is a novel mitochondrial regulator. EFHD1 has been reported in the progression of many types of cancer, such as osteosarcoma, clear cell renal cell carcinoma, and colorectal cancer. Our study aimed to investigate the mechanisms of EFHD1 in breast cancer (BRCA).

CTRP3 attenuates myocardial lipotoxicity via suppression of lipid accumulation, inflammation, apoptosis, and mitochondrial oxidative stress.

Unlabelled: Myocardial lipotoxicity, a pathophysiological condition characterized by cardiomyocyte damage resulting from dysregulated fatty acid metabolism, plays a pivotal role in cardiovascular disease progression. C1q/tumor necrosis factor-related protein-3 (CTRP3), a novel adipocytokine with pleiotropic metabolic regulatory properties, has recently been implicated in lipid homeostasis modulation. Nevertheless, its cardioprotective potential against myocardial lipotoxicity remains poorly understood.

Endolysosomal engulfment of autophagosomes independent of ESCRT.

Endolysosomes, considered the cellular recycling compartments, receive and degrade materials from multiple pathways. However, whether endolysosomes can acquire cargo through alternative mechanisms remains unclear. Here, we identify a previously unrecognized endolysosomal pathway for material uptake.

Exosomal delivery of AZD5582 to overcome TRAIL resistance as an optimal therapy against triple-negative breast cancer.

Triple-negative breast cancer (TNBC) is a highly aggressive subtype of breast cancer that lacks effective targeted therapies mainly due to drug resistance. Therefore, there is an urgent need to develop highly effective therapeutic strategies for TNBC. Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in transformed and cancerous cells, indicating its potential for anti-cancer therapy.

Injectable self-healing hydrogels loaded with Crinis Carbonisatus nanoparticles for rapid hemostasis and wound healing.

Uncontrolled hemorrhage and infection are leading causes of post-traumatic deaths in both clinical and battlefield settings. Although several wound dressings have been developed, rapid and effective hemostasis and wound healing promotion in irregular and non-compressible wounds remain serious challenges. In this study, a multifunctional hydrogel (HPCS-C/ODEX/CC hydrogel) was developed using two natural polysaccharide derivatives, catechol-modified hydroxypropyl chitosan (HPCS-C) and oxidized dextran (ODEX), as well as Crinis Carbonisatus (CC) nanoparticles for rapid hemostasis and wound healing.

Revising Representation and Target Deviations for Accurate Human Pose Estimation.

Owing to the normalized instance scales and robust supervision, heatmap-based human pose estimation (HPE) methods with top-down paradigm have achieved a dominant performance. However, there are two inherent deviations in the basic framework, i.e.

Sialyltransferase gene signature as a predictor of prognosis and therapeutic response in kidney renal clear cell carcinoma.

Background: Sialyltransferases are enzymes involved in the addition of sialic acid to glycoproteins and glycolipids, influencing various physiological and pathological processes. The expression and function of sialyltransferases in tumors, particularly in kidney renal clear cell carcinoma (KIRC) remained underexplored. This study aimed to develop a prognostic model based on sialyltransferase-related genes (SRGs) to predict the prognosis and treatment response of patients with KIRC.

Fatty acid synthesis promotes mtDNA release via ETS1-mediated oligomerization of VDAC1 facilitating endothelial dysfunction in sepsis-induced lung injury.

Sepsis involves endothelial cell dysfunction leading to the development of lung injury. Fatty acid synthesis contributes to the development of inflammatory injury in sepsis. However, the regulatory mechanisms of fatty acid synthesis-related endothelial activation remain unclear.

An advanced inhalable dry powder, mucus-penetrating aerosol platform: Bridging Andrographolide delivery with clinical translation.

Effective aerosol drug delivery remains a challenge for treating pulmonary diseases due to physiological barriers such as mucus accumulation, biofilm formation, and rapid macrophage clearance. Here, we developed an inhalable honeycomb-like microsphere (HCLplga-Ab) aerosol platform using FDA-approved poly(lactic-co-glycolic acid) (PLGA) and a pore-forming agent. The platform encapsulates Andrographolide, a bioactive compound derived from traditional Chinese medicine, together with a chitosan-ambroxol coating to achieve mucus penetration, sequential drug release, and prolonged retention in the lungs.