Dendrobin A inhibits gastric cancer: Mechanistic insights supported by integrated evidence.

Background: Traditional Chinese medicine (TCM) has shown anti-tumor potential, but its molecular mechanisms remain poorly understood. This integrated bioinformatics, network pharmacology, and experimental study investigated the anti-cancer effects and mechanisms of Dendrobin A, a pharmacologically active bibenzyl compound from Dendrobium nobile, in gastric cancer (GC).

Methods: Differentially expressed genes (DEGs) were identified through analysis of the TCGA-STAD dataset.

METTL1-mediated mG methylation of mRNA promotes macrophage inflammatory responses and multiple organ injury.

RNA modifications regulate phenotype and function of macrophages by regulating RNA translation, splicing, and stability. However, the role of -methylguanosine (mG) modification in macrophages and inflammation remains unexplored. In this study, we observed elevated levels of the methyltransferase METTL1 and mG modifications in macrophages from mouse and human tissues during acute kidney injury (AKI).

Brief introduction in phenotypic and genetic differences of C57BL/6 and BALB/c mice substrains.

Experimental mice play a critical role in biomedical research. The phenotype and application of different substrains vary due to genetic differentiation and variation. To ensure validity and reliability of results, it is imperative to adhere to standardized experiments and controls.

AuNP aggregation-triggered conformational changes in hairpin probes for dual-mode colorimetric and SERS detection of TTX.

Tetrodotoxin (TTX) is a potent neurotoxic marine biotoxin that poses severe health risks. To address this challenge, a dual-mode detection strategy was developed based on TTX-induced conformational changes in hairpin probes (HP), regulation of Au NPs aggregation to modulate localized surface plasmon resonance, and hotspot formation for colorimetric and surface-enhanced Raman spectroscopy (SERS) signal detection. This method demonstrates higher specificity than ion-induced aggregation.

Transcriptomic and Metabolomic Insights into the Effects of Arbuscular Mycorrhizal Fungi on Root Vegetative Growth and Saline-Alkali Stress Response in Oat ( L.).

Soil salinization limits the growth of agricultural crops in the world, requiring the use of methods to increase the tolerance of agricultural crops to salinity-alkali stress. Arbuscular mycorrhizal fungi (AMF) enhance plant stress adaptation through symbiosis and offer a promising strategy for remediation. However, in non-model crops such as oat ( L.

Bidirectional association of daily steps with sarcopenia: a longitudinal study.

Background: Step count is a well-established measure of physical activity, and step-based recommendations offering practical public guidance. However, the bidirectional associations between step count and sarcopenia remain unclear, limiting the application of step-based interventions for sarcopenia prevention. This study examined the directional association between daily steps and sarcopenia.

miR-361-5p regulates acute infective endocarditis by mediating SLPI/NF-κB signaling pathway.

Acute infective endocarditis (AIE) is an inflammatory reaction caused by the infection of the endocardial surface, accompanied by the formation of vegetation with different shapes and sizes. miR-361-5p and secretory leukocyte protease inhibitor (SLPI) were reported to mediate the process of staphylococcus aureus (SA) infection in AIE, but their roles in AIE are unclear. In this study, the AIE cell model was induced by hypoxia and infection of SA, and the AIE rat model was established.

The HCR signal amplification-based SERS aptasensor for highly sensitive detection of AFB1.

Aflatoxin B1 (AFB1) is a potent carcinogen that poses a serious threat to human health. Therefore, the rapid and accurate detection of AFB1 in grains is crucial. For a highly sensitive aptasensor for AFB1 detection, a method based on the hybridization chain reaction (HCR) and magnetic nanoparticles (FeO@Au NPs) strategy for signal amplification through infinite elongation of DNA strands was employed.

Toxic Effects of Povidone-Iodine on : Concentration-Dependent Responses in Oxidative Stress, Immunosuppression, and Recovery Potential.

Povidone-iodine (PVP-I), a widely used aquaculture disinfectant, remains poorly understood in terms of sublethal toxicity and damage reversibility. This study employed as the model organism to evaluate the acute toxicity and sublethal effects of PVP-I through a 4-day exposure experiment followed by a 7-day depuration period. Acute toxicity tests enabled the determination of 24-96 h median lethal concentrations (LC), with the 96 h LC being 5.

Association of playing cards/mahjong with all-cause mortality in older adults: a cohort study.

Background: Engagement in leisure activities such as playing cards or mahjong has been linked to cognitive and physical benefits among older adults. However, the relationship between playing cards/mahjong and all-cause mortality remains unclear. This study aims to explore the association between playing cards/mahjong and all-cause mortality in a cohort of older Chinese adults.

Effector 31812 from acts as avirulence factor for -mediated resistance in wheat.

As an obligate biotrophic fungus, the leaf rust pathogen secretes a repertoire of effector proteins into host cells for modulating plant immunity and promoting fungal pathogenesis. Here, we identify the Pt31812 effector and characterize its function in pathogenesis and immune-related activity in plants. In the study, was cloned by PCR, and the expression pattern and structure were analyzed by qRT-PCR and online softwares.

Plasma lipoprotein subclasses and risk of incident knee osteoarthritis: A population-based cohort study.

Objective: Lipoprotein subclasses play diverse roles in health and disease. Although lipoproteins have been implicated in knee osteoarthritis (KOA), the relationship between lipoprotein subclasses and KOA remains unexplored.

Method: We conducted a cohort study using UK Biobank data to examine the associations of 85 plasma lipoprotein subclasses, defined by combinations of density (high, low, intermediate, very-low), size (extremely-large, very-large, large, medium, small, very-small), and composition (triglyceride, free cholesterol, cholesteryl ester, phospholipid), with incident KOA in metabolome-wide analysis.

Sedimentation substantially buries more carbon than nitrogen or phosphorus in the Three Gorges Reservoir, China.

Sedimentation and the accompanying accumulation of nutrients such as total organic carbon (TOC), total nitrogen (TN) and total phosphorus (TP) pose threats to ecological stability and the sustainable operation of reservoirs. The Three Gorges Reservoir (TGR) in China is a mega river-type reservoir with substantial sedimentation since its impoundment, although the quantity of nutrients buried in the TGR is unknown. In the present study, the monthly suspended sediment loads at the TGR inlet (Cuntan and Beibei hydrologic stations) and outlet (Yichang hydrologic station) were collected during 2003-2023 to estimate the total sedimentation.

Self-Powered Photonic Sensor Using Cholesteric Liquid Crystal Elastomer-Based Sandwiched Architecture with Mechanochromism and Triboelectric Response.

Wearable flexible sensors have aroused great interest owing to their widespread use in medical monitoring, wearable sensing, and human-computer interaction. However, conventional flexible sensors have a single signal response and are susceptible to mechanical damage, thus limiting their potential applications. Here, a self-powered flexible sensor using a cholesteric liquid crystal elastomer (CLCE)-based sandwiched architecture with mechanochromism and triboelectric response is developed.

Inflammatory Biomarkers as Prognostic Indicators for Intracranial Aneurysm Recurrence After Stent-Assisted Coil Embolization.

Background: The reappearance of intracranial aneurysms (IAs) after undergoing stent-assisted coil embolization (SACE) is a significant issue in clinical practice. In this study, we analyzed blood regulatory T-cell counts and plasma cytokine levels to assess the extent of systemic inflammation and investigate their potential association with the recurrence of IAs undergoing SACE.

Methods: A total of 189 individuals with 220 unruptured IAs were included in a retrospective study, with participants categorized into groups of occlusion and recurrence according to the Raymond-Roy Scale.

Association of a Healthy Lifestyle With All-Cause and Cause-Specific Mortality Among Individuals With Probable Sarcopenia: Population-Based Cohort Study.

Background: Individuals with probable sarcopenia have shown excess mortality, yet no specific treatment regimen has been established. While lifestyle factors improve health and longevity in general populations, their role in probable patients with sarcopenia remains unclear due to differing lifestyle patterns. Clarifying this could inform strategies to address this unmet need.

Genetically engineered chondrocyte-mimetic nanoplatform attenuates osteoarthritis by blocking IL-1β and restoring sirtuin-3.

Osteoarthritis (OA) is a multifactorial disease characterized by joint inflammation and cartilage degeneration, with no disease-modifying drugs available. The vicious cycle between the inflammatory microenvironment (inflamed soil) and dysfunctional chondrocytes (degeneration-related seeds) drives the chronic progressive deterioration of OA. Here, we report a genetically engineered chondrocyte-mimetic nanoplatform (termed HKL-GECM@MPNPs) comprising a honokiol (HKL)-loaded mitochondrion-targeting nanoparticle core coated with an interleukin-1 receptor type 2 (IL-1R2)-overexpressing chondrocyte membrane.

Chromosome-level genome assembly of Qihe gibel carp.

Qihe gibel carp (Carassius gibelio var. Qihe) is a local population of natural gynogenetic amphitriploid (AAABBB) Carassius gibelio, and has high nutritional and economic value. In this study, we assemble a high-quality chromosome-level genome of Qihe gibel carp through DNBSEQ, PacBio HiFi, and Hi-C sequencing data.

Self-powered and dual-amplified photoelectrochemical sensing platform for highly sensitive detection of aflatoxin B.

Aflatoxin B (AFB) is highly toxic and commonly found in contaminated grains and their products; therefore, a rapid method for the detection of AFB is essential to safeguard public health. This study developed a portable photoelectrochemical (PEC) sensor using In(OH) and a hybridization chain reaction (HCR) enhanced by methylene blue (MB) for on-site AFB detection. In(OH) was immobilized on a screen-printed electrode, followed by gold nanoparticle deposition and capture probe attachment.

Path analysis and curve fitting of morphometric traits and body weight of juvenile Aspiorhynchus laticeps.

In this experiment, correlation analysis, path analysis, regression analysis and curve-fitting methods were employed to investigate the relationships between the body weight (Y) and morphological variables (X1-X36) of juvenile Aspiorhynchus laticeps (common name: big-head schizothoracin). These methods were used to identify the main morphological trait indices affecting body weight and further determine the optimal fitting models between four main morphological traits and body weight. The study found that 94% of the morphological traits of juvenile A.

Sensitivity-enhanced detection of ethanol gas using MoO nanoflake-functionalized laser-induced graphene in a planar microwave resonator.

Recent advancements in sensor technology have heightened the demand for more sensitive gas detection methods, crucial for improving environmental monitoring and industrial safety applications. This study investigates the potential of a hybrid of laser-induced graphene (LIG) and MoO nanoflakes via a planar microwave resonator for detecting ethanol gas with high sensitivity. To implement the proposed sensor, a rectangular-shaped graphene layer was patterned on a polyimide substrate using a computer-controlled CO laser and functionalized with MoO nanoflakes.

The effect of hormone replacement therapy on cervical cancer risk in perimenopausal women: a systematic review and meta-analysis of observational studies.

Background: Hormone replacement therapy (HRT) alleviates menopausal symptoms in perimenopausal women and help improve their quality of life, but its increased risk of cervical cancer (CC) remains to be evaluated.

Methods: A system review and meta-analysis was conducted to retrieve literature related to HRT and CC risk by searching Pubmed, Embase, Science Direct, Web of Science, and Google Scholar databases. After screening the literature according to inclusion criteria and assessing the risk of bias using the Newcastle Ottawa Scale, the odd ratio (OR) values of HRT relative to CC were pooled.

Riboflavin-mediated reactivation of aged nano zero-valent iron by Shewanella oneidensis MR-1 for sustained Cr(VI) detoxification.

Aging-induced surface passivation of nano zero-valent iron (nZVI) significantly diminishes its reactivity for Cr(VI) reduction, thereby impeding its practical application in environmental remediation. Herein, we propose a novel system integrating aged nZVI (AnZVI), Shewanella oneidensis MR-1, and the endogenous electron shuttle riboflavin (RF) to overcome the passivation limitations and enhance Cr(VI) removal. Remarkably, the AnZVI/MR-1/RF system achieved complete removal of 20 mg L Cr(VI) within 3 h, outperforming AnZVI/MR-1 and AnZVI alone by 23.

CD79A and GADD45A as novel immune-related biomarkers for respiratory syncytial virus severity in children: an integrated machine learning analysis and clinical validation.

Background: Respiratory syncytial virus (RSV) is a leading cause of severe lower respiratory infections in children, yet biomarkers for assessing disease severity remain limited. Herein, we investigated the differential expression biomarkers between RSV infected hospitalized patients, healthy groups and RSV infected outpatients.

Methods: Two publicly available transcriptomic datasets (GSE77087 and GSE188427) were retrieved from the Gene Expression Omnibus (GEO) database.

DNMT1 blocks SOX21-repressed CKS2 transcription to promote gastric cancer progression.

Background: The dysregulation of SOXs is related to tumor invasion, metastasis, proliferation, apoptosis, and epithelial-mesenchymal transition. This research sought to investigate the function and mechanisms of SOX21 in gastric cancer (GC).

Methods: Multiple databases were included to determine the hub transcription factors in GC.