Stage-Resolved Phosphoproteomic Landscape of Mouse Spermiogenesis Reveals Key Kinase Signaling in Sperm Morphogenesis.

Spermiogenesis is the committed step of sperm production, during which spermatid cells undergo dramatic morphological transformations and transcriptional silencing. Post-translational modifications (PTMs), including phosphorylation, provide a level of protein function flexibility and play important roles in spermiogenesis. Dynamic protein phosphorylation profiles of spermatids are characterized across four different developing steps, and identified phosphorylation regulation of key proteins in spermiogenesis.

The N-terminal region of HASPIN regulates phosphorylation of AURKA and meiotic progression in spermatocytes.

Protein phosphorylation is an important post-translational modification that plays a critical regulatory role in meiosis. HASPIN, a kinase highly conserved from yeast to mammals, is required for male fertility. In this study, we found that the intrinsically disordered N-terminal domain of HASPIN is also required for this function.

['s experience in treatment of post-stroke hypermyotonia with "trinity" approach of 's therapy].

This article introduces the clinical experience of Professor in treatment of post-stroke hypermyotonia with 's therapy (therapy for removing obstruction and consolidating vital essence). Regarding the pathogenesis of this disease, Professor emphasizes a "trinity" approach, "the mind disturbed, and blood unbalanced, and (vital essence) not consolidated". In treatment, "the unblocking, conducting and nourishing are taken as the fundamental" to "prevent from over-dredging, balancing and , and avoiding over-replenishing".

A functionally tunable magnetic nanochains platform for N-glycoproteomic analysis of extracellular vesicles from ultratrace biofluids.

Extracellular vesicles (EVs) carry complex glycoproteins critical mediators of disease progression and hold great potential for liquid biopsy applications. However, their low abundance presents significant analytical challenges. This study develop a versatile platform for EV N-glycoproteomic analysis (EVGpro), which enables tandem EV capture, EV protein digestion, and enrichment of N-glycopeptides.

HOX11 gene overexpression is associated with recurrence of acute B lymphoblastic leukaemia.

This research aimed to elucidate the association of Homeo Box 11 (HOX11) gene overexpression with refractory acute B-cell lymphoblastic leukaemia (B-ALL). In this investigation, 209 patients with B-ALL, who were treated in Guangdong Province between 2019 and 2024, were included. At the initial diagnosis, the RNA was obtained from bone marrow for genetic testing via Real-time Quantitative Polymerase Chain Reaction (RQ-PCR).

[Summary of 's experience in treating refractory lateral epicondylitis with crescent technique of blade needle].

This paper introduces Professor 's clinical experience in treating refractory lateral epicondylitis using the 's crescent technique of blade needle. Based on the classical theories of () such as the "twelve sinew meridians" "short needling" and " needling", Professor applies a self-designed blade needle with crescent needling method. This approach forms a distinctive treatment philosophy characterized by "sinew meridians as the foundation, selecting points at the pain site; using chicken-claw needling technique deliverd at both internal and external; integration of heaven, earth, and man, using both short and needling; treating tendon injuries and inflammation with the crescent technique of blade needle".

DICER1 mutations in Bethesda category II, III, and IV thyroid nodules: A mutually exclusive relationship with BRAF mutation.

Background: This study aimed to investigate the prevalence and cytological features of Dicer 1, Ribonuclease III (DICER)-mutant fine-needle aspiration (FNA) specimens in thyroid nodules classified as Bethesda II, III, and IV categories. The authors also sought to explore the relationship between DICER1 and BRAF mutations in Bethesda III thyroid nodules.

Methods: The authors collected a series of consecutive FNA cases diagnosed as Bethesda category II, III, and IV from a medical center over the course of 1 year.

Changes in the hydrological processes of glacial lake-fed rivers on the Tibetan Plateau due to climate change.

River hydrological processes in the Tibetan Plateau are highly sensitive to climate change; therefore, this region is ideal for studying climate-driven hydrological responses. The existing research on glacial lake-fed rivers is limited, due to the scarcity of hydrological observational data. To address this research gap, in this study, we focused on three river types (glacial lake-, precipitation-, and glacier-fed rivers) in the Yarlung Tsangpo Grand Canyon region in Tibet Autonomous Region, China.

Antimicrobial resistance and molecular characteristics of bovine mastitis-associated methicillin-resistant : potential for cross-species transmission of ST59-MRSA.

Unlabelled: Mastitis caused by methicillin-resistant (MRSA) is a common issue in dairy farming, with sequence types (STs) related to cows mainly including ST9 and ST97. ST59, the predominant community-acquired clone, is still less reported in dairy cows. This study investigated the antimicrobial resistance patterns and molecular characteristics of 77 isolates obtained from eight dairy farms in the mid-east of China during 2019-2020, focusing particularly on the bovine mastitis-related livestock-associated MRSA (LA-MRSA) clone ST59.

ICAT mediates the inhibition of stemness and tumorigenesis in acute myeloid leukemia cells induced by 1,25-(OH)D.

Background: The role of 1,25-dihydroxyvitamin D3 (1,25-(OH)D) in cancer prevention and treatment is an emerging topic of interest. However, its effects on the stemness of acute myeloid leukemia (AML) cells are poorly understood.

Methods: The proliferation and differentiation of AML cells (HL60 and NB4) were investigated by the CCK-8 assay, immunocytochemical staining, and flow cytometry.

A Broad-Spectrum Monoclonal Antibody-Based Heterologous ic-ELISA for the Detection of Multiple Pyrethroids in Water, Milk, Celery, and Leek.

Pyrethroids are one of the most commonly used insecticides worldwide in agriculture, public health, and household products. To monitor the presence of pyrethroids in the environment and in food, a broad-spectrum monoclonal antibody (mAb), CL/CN-1D2, was prepared. This mAb demonstrates a 50% inhibitory concentration (IC50) for different pyrethroids: cypermethrin (129.

Ecological flow evaluation method based on Habitat Diversity Index --An empirical study from the upper Yalong River in China.

Traditional ecological flow calculations often rely on species-specific habitat preferences, but this approach may adversely affect biodiversity conservation. This study proposes an innovative approach focused on habitat diversity, utilizing the Habitat Diversity Index (HDI) to evaluate ecological flows for benthic macroinvertebrate communities. By integrating fields surveys, hydrodynamic modeling, and biodiversity metrics, the study quantified habitat diversity through 24 distinct habitat types (combinations of water depth: 0-0.

The organelle genomes of the endangered seagrass reveal sequence divergences, massive gene transfer, and uncommon RNA editing types.

Introduction: , a rare submerged angiosperm, is considered endemic to the northwestern Pacific.

Methods: This study assembled and compared the mitochondrial (mt) and chloroplast (cp) genomes of to understand the organelle evolutionary patterns.

Results And Discussion: The cp genome (143,972 bp) was the second smallest within the seagrasses, whereas the mt genomes (192,246 bp) of and other seagrasses were smaller compared to those of other monocotyledons.

The Extra-Tumoral Vaccine Effects of Apoptotic Bodies in the Advancement of Cancer Treatment.

The induction of apoptosis in tumor cells is a common target for the development of anti-tumor therapies; however, these therapies still leave patients at increased risk of disease recurrence. For example, apoptotic tumor cells can promote tumor growth and immune evasion via the secretion of metabolites, apoptotic extracellular vesicles, and induction of pro-tumorigenic macrophages. This paradox of apoptosis induction and the pro-tumorigenic effects of tumor cell apoptosis has begged the question of whether apoptosis is a suitable cancer therapy, and led to further explorations into other immunogenic cell death-based approaches.

Drug-induced senescence of donor dermal fibroblasts enhances revascularization and graft success in skin transplantation.

Full-thickness skin grafts often face challenges related to inefficient vascularization in clinical settings. Senescent cells, known for secreting various growth factors, have demonstrated excellent effects on angiogenesis. In this study, we induced senescence in a subset of fibroblasts in the donor dermis by co-administering trametinib and palbociclib before harvesting the skin grafts for transplantation.

Development of a monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for screening of diethylstilbestrol in animal-derived foods.

Diethylstilbestrol (DES), a synthetic non-steroid estrogen, it has been prohibited from being added to animal feed for any purposes. Herein, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a specific monoclonal antibody (mAb) was developed for the rapid screening of DES. Primarily, conjugates of mono-O-3-carboxypropyl diethylstilbestrol with keyhole limpet hemocyanin were used to raise a specific mAb, 1B7, which had IC value for DES of 213.

A Lincomycin-Specific Antibody Was Developed Using Hapten Prediction, and an Immunoassay Was Established to Detect Lincomycin in Pork and Milk.

Prolonged consumption of animal-derived foods containing high levels of lincomycin (LIN) residues can adversely impact human health. Therefore, it is essential to develop specific antibodies and immunoassay methods for LIN. This study utilized computational chemistry to predict the efficacy of LIN haptens prior to chemical synthesis, with subsequent confirmation obtained through an immunization experiment.

Reversible Electrochemical Swelling of Straight Carbon Nanotube Yarns for High-Performance Linear Actuation.

Coiled artificial muscle yarns outperform their straight counterparts in contractile strokes. However, challenges persist in the fabrication complexity and the susceptibility of the coiled yarns to becoming stuck by surrounding objects during contraction and recovery. Additionally, torsional stability remains a concern.

Sodium alginate-based coaxial fibers synergistically integrate moisture actuation, length tracing, humidity sensing, and electric heating.

The development of wearable electronics has driven the need for smart fibers with advanced multifunctional synergy. In this paper, we present a design of a multifunctional coaxial fiber that is composed of a biopolymer-derived core and an MXene/silver nanowire (AgNW) sheath by wet spinning. The fiber synergistically integrates moisture actuation, length tracing, humidity sensing, and electric heating, making it highly promising for portable devices and protective systems.

Engineering an energy-dissipating hybrid tissue in vivo for obesity treatment.

Obesity is a global health challenge with limited therapeutic solutions. Here, we demonstrate the engineering of an energy-dissipating hybrid tissue (EDHT) in the body for weight control. EDHT is constructed by implanting a synthetic gel matrix comprising immunomodulatory signals and functional cells into the recipient mouse.

Comprehensive analysis of human monocyte subsets using full-spectrum flow cytometry and hierarchical marker clustering.

Introduction: Exploring monocytes' roles within the tumor microenvironment is crucial for crafting targeted cancer treatments.

Methods: This study unveils a novel methodology utilizing four 20-color flow cytometry panels for comprehensive peripheral immune system phenotyping, specifically targeting classical, intermediate, and non-classical monocyte subsets.

Results: By applying advanced dimensionality reduction techniques like t-distributed stochastic neighbor embedding (tSNE) and FlowSom analysis, we performed an extensive profiling of monocytes, assessing 50 unique cell surface markers related to a wide range of immunological functions, including activation, differentiation, and immune checkpoint regulation.

Protective Effect of Vitamin K2 (MK-7) on Acute Lung Injury Induced by Lipopolysaccharide in Mice.

Vitamin K2 (MK-7) has been shown to cause significant changes in different physiological processes and diseases, but its role in acute lung injury (ALI) is unclear. Therefore, in this study, we aimed to evaluate the protective effects of VK2 against LPS-induced ALI in mice. The male C57BL/6J mice were randomly divided into six groups (n = 7): the control group, LPS group, negative control group (LPS + Oil), positive control group (LPS + DEX), LPS + VK2 (L) group (VK2, 1.

A mechanism study on the synergistic effects of rifapentine and fluconazole against fluconazole-resistant .

() is one of the most common clinical isolates of systemic fungal infection. Long-term and inappropriate use of antifungal drugs can cause fungal resistance, which poses a great challenge to the clinical treatment of fungal infections. The combination of antifungal drugs and non-antifungal drugs to overcome the problem of fungal resistance has become a research hotspot in recent years.