Impact of a nurse-led multidomain intervention on intrinsic capacity among community-dwelling older adults in China: a cluster randomized trial.

Background: In China, community-dwelling older adults with intrinsic capacity (IC) impairments often turn first to primary care centers, where they face increasingly complex health needs. Given the high prevalence of IC impairment among older adults in Chinese communities, there is a pressing need to explore context-specific, nurse-led multi-domain interventions to support functional ability and healthy aging.

Design: A parallel-group, cluster randomized trial conducted in September 2023, involving 86 older adults from two distinct communities.

Capsid structure of phage SPO1 reveals novel minor capsid proteins and insights into capsid stabilization.

SPO1-related bacteriophages are promising candidates for phage therapy. We present the 3.0 Å cryo-electron microscopy (cryo-EM) structure of the SPO1 capsid with a triangulation number T = 16, enabling the construction of an atomic model comprising the major capsid protein and three types of minor capsid proteins: gp29.

A BTK-activatable targeted covalent NIR fluorescent probe for kinase monitoring and B-cell lymphoma imaging.

B-cell lymphoma is one of the most aggressive malignancies worldwide. Bruton's tyrosine kinase (BTK), a crucial mediator of B-cell receptor (BCR) signaling pathway, has emerged as a pivotal therapeutic target for B-cell lymphoma, so developing a molecular probe capable of quantitatively monitoring BTK expression and activity in live cells would be highly desirable for the early diagnosis and therapy of B-cell lymphoma. Here, we have constructed a BTK-activatable NIR fluorescent (NIRF) probe (Ibt-CyOH), which features a targeting ligand of ibrutinib as a recognition unit, a NIRF reporter, and an α-methylated acrylamide as a cleavable linker, triggered by nucleophilic addition-elimination reactions.

Wearable electrochemical immunosensor based on ultra-thin flexible stainless steel sheets for detection of methyl jasmonate in tomato leaves.

Methyl jasmonate (MeJA), a key plant hormone, plays essential roles in plant growth, development, biotic stress responses, and wound-induced defense. Monitoring dynamic changes in MeJA in situ is vital for botanical research. Herein, coupling with paper-based analytical devices, the ultra-thin flexible stainless steel sheets with the excellent flexibility and conductivity were used to develop wearable electrochemical immunosensor for in situ and continuous detection of MeJA in plants.

METTL14 promotes hippocampal neuronal cuproptosis via mA modification on FDX1 mRNA in cerebral ischemia-reperfusion injury.

Objective: Cuproptosis, a consequence of excessively high copper concentrations, is considered a potential therapeutic target for cerebral ischemia-reperfusion injury (IRI). Methyltransferases are key modulators of cuproptosis through N6-methyladenosine (mA) modification on ferredoxin 1 (FDX1) mRNA. This study aims to determine whether methyltransferase-like 14 (METTL14) regulates cuproptosis in cerebral IRI via mA modification on FDX1 mRNA.

Regulation of working memory switches from striatal dopamine D2-receptor to D1-receptor neurons under high cognitive load.

Working memory (WM) is a fundamental cognitive function crucial adaptive behavior. The intricate interplay between the frontal cortex and striatum in governing WM maintenance and updating remains a central question. In this study, we employed optogenetics to demonstrate that inhibiting both dorsomedial striatum (DMS) D1R- and D2R-neurons enhances WM, while their activation impairs it across T-maze and operant-based delayed-non-match-to-place (DNMTP) paradigms in mice.

Krill oil alleviates type 2 diabetes mellitus-induced sarcopenia in mice attenuating insulin resistance, intestinal barrier dysfunction, and skeletal muscle protein turnover impairment.

Krill oil (KO), a source of EPA, DHA, phospholipids, and astaxanthin, has emerged as a promising functional ingredient to maintain skeletal muscle health. However, its protective role against type 2 diabetes mellitus (T2DM)-induced sarcopenia has remained poorly characterized. Hence, the present study aimed to investigate the protective effects and underlying mechanisms of KO against sarcopenia in both a T2DM mouse model induced by the combination of streptozotocin and high-fat, high-sucrose diet, and a C2C12 myotube atrophy model induced by high glucose (HG), advanced glycation end products (AGEs), and lipopolysaccharide (LPS).

The association between dietary fiber intake and pelvic inflammatory disease in women: findings from the NHANES 2015-2018.

Background And Aim: Pelvic inflammatory disease (PID) is a common and serious gynecological condition caused by ascending infections of the female genital tract. It affects approximately one million women annually in the United States and is a leading cause of infertility, ectopic pregnancy, and chronic pelvic pain. While well-established risk factors include multiple sexual partners and sexually transmitted infections, emerging evidence suggests that dietary factors may also influence systemic and reproductive tract inflammation.

Lysosome Escape and Golgi Apparatus Accumulation Drive Robust Pyroptosis Based on Sulfhydryl-Inspired Aggregation-Induced Emission Photosensitizers.

The prolonged residence time of photosensitizers (PSs) at the subcellular compartment was proposed as a smart approach to enhance the efficiency of oxidative damage in photodynamic therapy. However, before reaching an interested organelle, small molecules during the internalization process suffered acidic conditions and hydrolases in lysosomes, which finally hindered more than 90% when arriving into targets. Herein, we synthesized a small-molecule PS (BTF-DNBS) with GSH-activatable tumoral identification, lysosome-escape, and Golgi apparatus-triggered pyroptosis.

Inetetamab triggers cardiotoxicity through its interaction with apoptosis, oxidative stress and autophagy pathways.

Inetetamab, a trastuzumab biosimilar, has obtained approval for the treatment of HER2-positive advanced metastatic breast cancer. Despite studies indicating its cardiotoxic properties, the intricate mechanisms underlying its toxicity remain elusive. In the present study, we demonstrated in vitro that exogenous administration of Inetetamab triggered injury in H9c2 myocardial cells, characterized by a significant decrease in cell viability and noticeable morphological changes.

Construction of balanced-transition AIE phototherapeutic agents based on a fluorination strategy for prolonged triplet excited state lifetime.

To solve the competitive energy decay pathways in photodynamic therapy (PDT) and photothermal therapy (PTT), herein, we proposed a fluorination strategy of AIE photosensitizers, which exhibited high ROS generation and heat release. Innovatively, femtosecond transient absorption spectroscopy has emerged to verify the extension of triplet excited state lifetime induced by C-F bonds. In order to improve its hydrophilicity and targeting ability, GSH-responsive nanogel HPCF was constructed, which presented controllable release of TPAF.

A self-adhesive hierarchical nanofiber patch for dynamic and multistage management of full-thickness cutaneous wounds.

Full-thickness cutaneous wounds pose a significant threat to global health due to their complex healing demands. Standard clinical wound dressings often fall short in providing the adaptability and functionality required for the entire healing process. While hierarchically engineered nanofiber dressings have shown advancement in wound management, challenges such as material compatibility and interfacial bonding during their design have limited both manufacturing and therapeutic outcomes.

Antibody-Directed Cell Internalization of Targeted Covalent Europium Tag Enables In Situ Kinase Labeling and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Quantification.

Development of methods to accurately detect the expression and activity of protein targets inside live cells is of great significance for disease diagnostics and drug development. Inductively coupled plasma mass spectrometry (ICP-MS) has been demonstrated as a promising technology for protein quantification, the key of which is developing element tags to selectively label the specific protein targets in complex samples with elements sensitive in ICP-MS. Numerous small-molecule element tags (SM-ETs) using DOTA as a lanthanide (Ln) chelating agent have been developed for specific protein target labeling.

Correction: Differential effects of EPA and DHA on aging-related sarcopenia in mice and possible mechanisms involved.

Correction for 'Differential effects of EPA and DHA on aging-related sarcopenia in mice and possible mechanisms involved' by Zi-Jian Wu , , 2025, , 601-616, https://doi.org/10.1039/D4FO04341C.

An antisense RNA forms R-loop to facilitate the transcription of CBF genes and plant cold acclimation.

Cold acclimation is an adaption used by many plants to survive freezing temperatures and involves rapid induction of the expression of three C-REPEAT-BINDING FACTOR (CBF) genes. We have previously annotated long non-coding RNAs (lncRNAs) including natural antisense transcripts (NAT-lncRNAs) on a genome-wide scale in Arabidopsis and identified a NAT-lncRNA transcribed from the antisense strand between CBF1 and CBF3. We named it CAS for CBF antisense transcript.

Unraveling gut microbiome alterations and metabolic signatures in hereditary transthyretin amyloidosis.

Unlabelled: Hereditary transthyretin amyloidosis (hATTR) is a rare, often fatal disease characterized by the abnormal aggregation of atypical transthyretin fibrils. Given the variability in the penetrance and clinical manifestations of hATTR, the role of nongenetic factors, particularly those related to the gut microbiota, warrants investigation. We conducted a cross-sectional study, examining the untargeted serum metabolome and gut metagenome in 13 patients with hATTR and 22 healthy controls.

Occurrence, hydrolysis and photodegradation of phthalate esters in urban atmospheric aqueous aerosol in Wuhan City.

A gas chromatography-flame ionization detection method was developed to investigate the occurrence and transformations of phthalate esters (PAEs) in atmospheric aqueous aerosol in Wuhan City. Six of PAEs were detected in collected aerosol samples and their concentrations in atmosphere were followed the order of diallyl phthalate (0.244 μg m) > diethyl phthalate (0.

Cross-protection and cross-feeding mediated by signaling molecules enhanced the viability of P. fluorescens S01 and R. erythropolis S02 under the dual stresses of polycyclic aromatic hydrocarbons (PAHs) and low temperature.

In harsh environments, microbes employ varieties of strategies, including cross-feeding and biofilm formation, to enhance their survival. Signaling molecules played a regulatory role in these processes. However, it remains unclear whether cross-feeding and biofilm formation can offer protection to others and precise regulatory mechanisms of signaling molecules in facilitating cross-protection.

Transferrin Receptor-Targeted Aptamer-Drug Conjugate Overcomes Blood-Brain Barrier for Potent Glioblastoma Therapy.

Glioblastoma (GBM) is the leading primary malignant tumor in the central nervous system. Current clinical therapeutics for treating GBM patients yield limited benefits. However, the development of new therapeutic methods is hindered because the blood-brain barrier (BBB) restricts drug penetration.

CsRAP2-7 negatively regulates cuticular wax biosynthesis and drought resistance in citrus by directly activating CsACO1.

Cuticular wax plays an important role in enhancing plant stress tolerance. While positive regulators of cuticular wax biosynthesis are well-studied, negative regulators remain largely unexplored in citrus. In the present paper, we screened and cloned an AP2/ERF family gene, CsRAP2-7, from navel orange.

The Extract of L. Reverses Pro-inflammatory Status of Microglia for Neuroprotection Following Ischemic Stroke in Mice.

Background: (SO) is a traditional Chinese herbal medicine commonly used for inflammatory diseases. In ancient, SO is applied for stroke, but mechanisms have not been clear. The purpose is to investigate whether SO exerts neuroprotective effects by reducing microglia-related inflammatory injury after focal cerebral ischemia/reperfusion (I/R).

Remote Vital Sensing in Clinical Veterinary Medicine: A Comprehensive Review of Recent Advances, Accomplishments, Challenges, and Future Perspectives.

Remote vital sensing in veterinary medicine is a relatively new area of practice, which involves the acquisition of data without invasion of the body cavities of live animals. This paper aims to review several technologies in remote vital sensing: infrared thermography, remote photoplethysmography (rPPG), radar, wearable sensors, and computer vision and machine learning. In each of these technologies, we outline its concepts, uses, strengths, and limitations in multiple animal species, and its potential to reshape health surveillance, welfare evaluation, and clinical medicine in animals.

Fast decomposition of typical endocrine disturbing compounds in microdroplets under sunlight irradiation in the presence of ozone.

Water microdroplets are ubiquitous in atmospheric environment, where photo-irradiation and O are available, while photo-decomposition of trace organic pollutants in microdroplets and the impact of atmospheric environmental factors on the photo-chemistry remain unclear. In this study, photochemical generation of reactive oxygen species and degradation of typical endocrine disturbing compounds (EDCs) in microdroplets were investigated under various atmospheric conditions (O, inorganic anions and fulvic acid). The experimental results demonstrate that EDCs can be degraded in microdroplets, and the degradation efficiency is further improved under the stimulated solar irradiation.

Computational insights into exploring the potential effects of environmental contaminants on human health.

With rapid industrialization and urbanization, the increasing prevalence of air and water pollutants poses a significant threat to public health. Traditional research methods, such as epidemiological studies and in vitro/in vivo experiments, provide valuable biological insights but are often costly, time-consuming, and limited in scale. To address this gap, this study develops a machine learning-based approach to predict the carcinogenicity of pollutants.

The Hydrochemical Characteristics and Evolution Process of Coal Reservoir Drainage Water in the Fukang Mining Area.

Coalbed water participates in the whole process of coalbed gas generation, migration, accumulation, and exploitation, and its hydrogeochemical characteristics play a crucial role in the exploration and development of coalbed gas in mining areas. Taking the Fukang mining area in Xinjiang as the research object, this study tested the conventional cations, anions, trace elements, pH value, and TDS of its aquifer and analyzed the hydrochemical characteristics and evolution process of coalbed gas drainage water. The results show that the drainage water of coalbed gas is generally weakly alkaline, with significant changes in TDS and ion concentrations over time.