Wearable electrochemical immunosensor based on ultra-thin flexible stainless steel sheets for detection of methyl jasmonate in tomato leaves.

Methyl jasmonate (MeJA), a key plant hormone, plays essential roles in plant growth, development, biotic stress responses, and wound-induced defense. Monitoring dynamic changes in MeJA in situ is vital for botanical research. Herein, coupling with paper-based analytical devices, the ultra-thin flexible stainless steel sheets with the excellent flexibility and conductivity were used to develop wearable electrochemical immunosensor for in situ and continuous detection of MeJA in plants.

Electrochemical sensors for plant signaling molecules.

Plant signaling molecules can be divided into plant messenger signaling molecules (such as calcium ions, hydrogen peroxide, Nitric oxide) and plant hormone signaling molecules (such as auxin (mainly indole-3-acetic acid or IAA), salicylic acid, abscisic acid, cytokinin, jasmonic acid or methyl jasmonate, gibberellins, brassinosteroids, strigolactone, and ethylene), which play crucial roles in regulating plant growth and development, and response to the environment. Due to the important roles of the plant signaling molecules in the plants, many methods were developed to detect them. The development of in-situ and real-time detection of plant signaling molecules and field-deployable sensors will be a key breakthrough for botanical research and agricultural technology.

Continuous In Vivo Monitoring of Indole-3-Acetic Acid and Salicylic Acid in Tomato Leaf Veins Based on an Electrochemical Microsensor.

Indole-3-acetic acid (IAA) and salicylic acid (SA), as critical plant hormones, are involved in multiple physiological regulatory processes of plants. Simultaneous and continuous in vivo detection of IAA and SA will help clarify the mechanisms of their regulation and crosstalk. First, this study reports the development and application of an electrochemical microsensor for simultaneous and continuous in vivo detection of IAA and SA.