Targeting the NAT10/XIST/YAP1 Axis-Mediated Vascular Abnormalization Enhances Immune Checkpoint Blockade in Gastric Cancer.

Tumor vascular normalization has emerged as a promising strategy to potentiate immune checkpoint blockade in solid tumors. Here, we unveil a previously unrecognized NAT10/XIST/YAP1/VEGFA signaling axis driving vascular abnormalization in gastric cancer (GC) and demonstrate its therapeutic potential in remodeling the tumor immune microenvironment. Through integrative analysis of acetylated RNA immunoprecipitation sequencing (acRIP-seq) and functional validation, we identified NAT10-mediated N4-acetylcytidine (ac4C) modification as a critical stabilizer of lncRNA XIST.

Epidemiology and Pathogenicity Analysis Based on Partial Recombinant PRRSV Strains in China.

Currently, multiple recombinant variants of porcine reproductive and respiratory syndrome virus (PRRSV) are circulating in China. These variants exhibit differences in pathogenicity. To comprehensively understand the current status of the virus and its recombination patterns, a total of 677 PRRSV strains were used for evolutionary analysis, of which 673 strains were isolated from mainland China.

Efficacy and safety of neoadjuvant toripalimab plus chemotherapy in localized deficient mismatch repair/microsatellite instability-high gastric or esophagogastric junction adenocarcinoma (NICE): a multicentre, single-arm, exploratory phase 2 study.

Background: In locally advanced gastric or gastroesophageal junction adenocarcinoma (GC/EGJC), deficient mismatch repair/microsatellite instability-high (dMMR/MSI-H) tumors exhibit high responsiveness to immunotherapy. The synergistic efficacy of neoadjuvant immunotherapy combined with chemotherapy in dMMR/MSI-H GC/EGJC remains uncertain.

Methods: The NICE trial is a multicentre, single-arm, exploratory phase 2 study conducted at six hospitals in China, evaluating the safety and efficacy of toripalimab in combination with CapeOX as perioperative therapy for locally advanced GC/EGJC across three biomarker-defined cohorts.

UBAP2L-driven stress granule formation links oxaliplatin resistance to gastric cancer.

Stress granules (SGs), which are phase-separating organelles that serve as protective cellular mechanisms in response to various harmful stimuli, have an unclear role in oxaliplatin resistance. Here, we establish a causal link between SG formation and oxaliplatin resistance in GC. Notably, we identify a key SG nucleator, namely, ubiquitin-associated protein 2-like (UBAP2L), as a previously unrecognized critical factor in mediating oxaliplatin resistance.

Fractionation mechanisms of rare earth element speciation in granitic weathering profiles: Metallogenic implications for the kuanyu ion-adsorption REE deposit, dechang, SW China.

The Kuanyu ion-adsorption rare earth element (REE) deposit, Sichuan's first economically viable resource of its type, remains underexplored in terms of REE fractionation and enrichment mechanisms within its weathering profiles. This study systematically resolves REE speciation patterns and light-to-heavy REE (LREE/HREE) differentiation processes, advancing the metallogenic framework fo r such deposits. Analytical results demonstrate: (1) A vertically progressive enrichment of LREE-dominated REE concentrations from bedrock to topsoil, with the fully weathered layer serving as the primary enrichment zone; (2) Weathering of primary REE-bearing minerals and subsequent secondary mineral formation as drivers of elemental redistribution; (3) Contrasting controls by clay minerals, iron-manganese oxides, and humic acids-clay minerals preferentially adsorb LREEs, while iron-manganese oxides exhibit stronger HREE affinity through inner-sphere complexation, and humic acids enhance HREE mobility via stable complex formation.

Multimodal radiopathomics approach for predictions of prognosis and immunotherapy response in patients with GC: a multicohort retrospective study.

Background: Current TNM staging systems insufficiently predict individual prognosis and chemotherapy response in gastric cancer (GC). We aimed to develop and validate a radiopathomics signature (RPS) integrating computed tomography (CT) and pathological features to improve prognostic stratification and guide treatment decisions. Preliminary evaluation of immunotherapy response was also conducted.

TME-guided deep learning predicts chemotherapy and immunotherapy response in gastric cancer with attention-enhanced residual Swin Transformer.

Adjuvant chemotherapy and immune checkpoint blockade exert quite durable anti-tumor responses, but the lack of effective biomarkers limits the therapeutic benefits. Utilizing multi-cohorts of 3,095 patients with gastric cancer, we propose an attention-enhanced residual Swin Transformer network to predict chemotherapy response (main task), and two predicting subtasks (ImmunoScore and periostin [POSTN]) are used as intermediate tasks to improve the model's performance. Furthermore, we assess whether the model can identify which patients would benefit from immunotherapy.

Multimodal radiopathomics signature for prediction of response to immunotherapy-based combination therapy in gastric cancer using interpretable machine learning.

Immunotherapy has become a cornerstone in the treatment of advanced gastric cancer (GC). However, identifying reliable predictive biomarkers remains a considerable challenge. This study demonstrates the potential of integrating multimodal baseline data, including computed tomography scan images and digital H&E-stained pathology images, with biological interpretation to predict the response to immunotherapy-based combination therapy using a multicenter cohort of 298 GC patients.

Targeting SPHK1 in macrophages remodels the tumor microenvironment and enhances anti-PD-1 immunotherapy efficacy in colorectal cancer liver metastasis.

Background: Colorectal cancer liver metastasis (CRLM) is characterized by an immunosuppressive microenvironment and a blunted response to immunotherapy. Notably, tumor-associated macrophages (TAMs) play a critical role in modulating immune responses and exhibit significant heterogeneity in CRLM. Sphingosine kinase 1 (SPHK1) serves as a pivotal kinase in maintaining the balance between ceramide and sphingosine-1-phosphate (S1P) levels.

Long-term outcomes of laparoscopic distal gastrectomy for locally advanced gastric cancer: An individual patient data meta-analysis of KLASS-02 and CLASS-01 randomized controlled trials.

Objective: Laparoscopic distal gastrectomy (LDG) has potential as a surgical treatment option for locally advanced gastric cancer (LAGC). However, there is uncertainty regarding the generalizability of LDG efficacy across diverse patient populations and treatment settings. This study aimed to assess the outcomes of LDG .

Automated cell annotation and classification on histopathology for spatial biomarker discovery.

Histopathology with hematoxylin and eosin (H&E) staining is routinely employed for clinical diagnoses. Single-cell analysis of histopathology provides a powerful tool for understanding the intricate cellular interactions underlying disease progression and therapeutic response. However, existing efforts are hampered by inefficient and error-prone human annotations.

Cholesterol confers resistance to Apatinib-mediated ferroptosis in gastric cancer.

Background: Gastric cancer is the fifth leading cause of cancer-related deaths worldwide. Apatinib is a third-line treatment for gastric cancer. However, the development of resistance significantly limits its efficacy, and effective and safe strategies to overcome Apatinib resistance remain elusive.

Conversion Surgery After Trastuzumab-Based Chemotherapy and Immunotherapy for Stage IV HER2-Positive Gastric Cancer: A Retrospective Multicenter Cohort Study.

Background: The efficacy and safety of conversion surgery after immunotherapy combined with trastuzumab-based chemotherapy for patients with stage IV human epidermal growth factor receptor 2 (HER2)-positive gastric cancer (GC) remains unclear.

Methods: This retrospective multicenter study included patients with stage IV HER2-positive GC treated between February 2012 and October 2024 across three centers in China. Eligible patients had histologically confirmed HER2-positive GC with unresectable stage IV disease treated with trastuzumab-based chemotherapy and/or immunotherapy.

Hydrogels in Veterinary Vaccine Development: Types, Mechanisms, and Applications.

This review examines the potential and challenges of using hydrogel vaccine delivery systems in animal immunization. Traditional methods face issues like low immunogenicity, reliance on cold chains, and inefficient delivery, limiting their use in modern animal husbandry. Hydrogels offer a promising solution due to their biocompatibility, controlled drug release, and immune regulation.

Bi-regional and bi-phasic automated machine learning radiomics for defining metastasis to lesser curvature lymph node stations in gastric cancer.

Background: Lymph node metastasis (LNM) is the primary metastatic mode in gastric cancer (GC), with frequent occurrences in lesser curvature. This study aims to establish a radiomic model to predict the metastatic status of lymph nodes in the lesser curvature for GC.

Methods: We retrospectively collected data from 939 gastric cancer patients who underwent gastrectomy and D2 lymphadenectomy across two centers.

Intraoperatively preventive intraperitoneal perfusion chemotherapy with lobaplatin in colorectal cancer: a prospective, randomised, controlled, multicentre study.

Background: Peritoneal metastasis (PM) after radical surgery is an important cause of treatment failure in colorectal cancer (CRC). Intraoperative intraperitoneal perfusion chemotherapy may be an effective method for preventing postoperative PM in patients with CRC. This study aimed to explore the safety and feasibility of intraoperatively preventive intraperitoneal perfusion chemotherapy using lobaplatin for CRC.

Dynamic key vascular anatomy dataset for D2 lymph node dissection during laparoscopic gastric cancer surgery.

Gastric cancer (GC) is the fifth most common malignant tumor worldwide. Surgical resection remains the primary treatment for GC, with laparoscopic surgery recommended by several international guidelines. Due to complex perigastric vessels, standard D2 lymph node dissection (LND) in laparoscopic GC (LapGC) surgery is challenging.

Safety and efficacy of indocyanine green-guided laparoscopic lymphadenectomy for locally advanced gastric cancer: The CLASS-11 clinical trials.

We report the short-term results of indocyanine green (ICG)-guided laparoscopic lymphadenectomy for gastric cancer (GC). The primary outcome is 3-year disease-free survival. In this analysis, we present short-term secondary outcomes focused on the number of lymph nodes (LNs) retrieved and the diagnostic value of fluorescent status for metastatic LNs, excluding long-term outcomes.

Toxic effects of co-exposure to polystyrene nanoplastics and arsenic in zebrafish (Danio rerio): Oxidative stress, physiological and biochemical responses.

The issue of nanoplastics (NPs) in the aquatic environment has recently received considerable attention. Arsenic (As) is a relatively abundant and toxic metalloid element in aquatic environments. However, the potential toxic effects of As on aquatic organisms under the influence of NPs remain uncertain.

Identification of Laminar Structure in the Yingxiongling Shale Oil Sediment in China with Random Forests and SHAP Analysis.

In this study, a diagnostic machine learning approach is proposed for automatic identification of laminar structures in sediments. The Yingxiongling shale oil in the Qaidam Basin of NorthWest China is used as a case study. First, various experimental methods were used to investigate the characterization of shale hydrocarbon potential, storage and seepage capacity, pore structure, and compressibility properties by sedimentary structures.

Isolation and Genomic Characteristics of a Novel Pathogenicity Type I Feline Coronavirus in Mainland China.

Feline coronavirus (FCoV) is an enveloped, positive-sense RNA virus, which is widespread among feline populations, and can cause a fatal serious disease called feline infectious peritonitis (FIP). According to the differences of antigen and genetic composition, FCoV consists of two genotypes, FCoV I and FCoV II. In this study, we have isolated and identified a FCoV I strain named HL2019.

Joint Nasogastric Tube Versus Traditional Decompression Nasogastric Tube to Guided OGT-Overlap Esophagojejunostomy in Laparoscopic Total Gastrectomy: A Randomized Controlled Trial.

Background: Our team pioneered the use of a joint nasogastric tube (JNT) for pairing overlap-esophagojejunostomy guiding tube (OGT) in an OGT-overlap esophagojejunostomy, demonstrating its safety and efficacy.

Objective: We aimed to compare the safety and efficacy of a novel technique, the JNT, with traditional nasogastric tubing (TNT) in guiding OGT-overlap esophagojejunostomy.

Methods: From January 2023 to January 2024, a total of 108 gastric/gastroesophageal junction (G/GEJ) cancer patients undergoing laparoscopic total gastrectomy (LTG) were eligible for inclusion in this study; however, 2 patients were excluded as they underwent hyperthermic intraperitoneal chemotherapy (HIPEC) after surgery.

Dynamic Locomotion Synchronization and Fuzzy Control of a Lower Limb Exoskeleton With Body Weight Support for Active Following Human Operator.

Despite remarkable progress in robotic exoskeletons, exoskeletons are still far from matching human-level guidance and locomotion performance in gait training or movement enhancement. A desirable exoskeleton would first provide a standard gait profile by learning from human operators while requiring body weight support with active human-following to govern dynamic locomotion synchronization. To address these issues, in this article, we propose a human operator-involved dynamic locomotion synchronization control framework for the lower limb exoskeleton actively following gait training with gravity-supporting.

The alkylglycerone phosphate synthase sustains the resistance of gastric cancer cells to ferroptosis induced by Apatinib.

Background: Apatinib is a targeted therapy used in the treatment of advanced gastric cancer. However, many gastric cancer patients develop resistance to Apatinib, and the mechanisms underlying this resistance remain unclear. Previous studies have shown that Apatinib can induce ferroptosis in gastric cancer cells.