Clinical and hemodynamic outcomes of side-to-side anastomosis in superficial temporal artery-middle cerebral artery bypass for adult patients with moyamoya disease: a prospective cohort study.

Objective: Superficial temporal artery-middle cerebral artery (STA-MCA) bypass, characterized by side-to-side (S-S) anastomosis, has been beneficial in reducing the incidence of postoperative complications and recurrent stroke in patients with moyamoya disease (MMD). However, the safety and efficacy of this unconventional S-S procedure remain unclear. This research aimed to investigate the clinical and hemodynamic outcomes associated with the S-S technique.

MicroRNA-301a directly targets IL-4/13A to negatively regulate Th2 immune responses in large yellow croaker (Larimichthys crocea).

Interleukin-4 (IL-4) and IL-13, predominantly secreted by T helper type 2 (Th2) cells, are pivotal cytokines involved in the Th2 response via the MAPK and mTOR signaling pathways. MicroRNAs (miRNAs) modulate various biological processes by inhibiting mRNA translation or facilitating mRNA degradation. However, research on miRNAs targeting IL-4 and IL-13 and their associated downstream signaling molecules remains limited.

Enhancing fibroblast-based bone regeneration by harnessing nanosecond pulsed electric field.

Despite their terminally differentiated state, human dermal fibroblasts (HDFs) can undergo osteogenic differentiation in vivo under certain pathological conditions, making them promising candidates for bone tissue engineering-though replicating this in vitro would be difficult. Building on prior findings that low-intensity (5 kV/cm) nanosecond pulsed electric fields (nsPEF) can partially reprogram HDFs toward pluripotency and boost their osteogenic capacity, an in vivo bone regeneration complex was fabricated by encapsulating nsPEF-treated cells in a self-healing hydrogel composed of oxidized hyaluronic acid and hydroxypropyl chitosan. In nude mice, these HDFs produced more robust ectopic bone both subcutaneously and within cranial defects, with significantly higher histological scores than untreated controls.

The surface nutrients in China's coastal waters in the winter of 2025.

Nutrients are the material basis of marine ecosystems, and understanding their levels and structures is of great significance for fully understanding marine ecosystems. Based on a survey of surface water in China's coastal waters in January 2025, this study explored the spatial heterogeneity and controlling factors of nutrient distribution in different areas of China's coastal waters. The results showed that there are significant regional variations in nutrient levels and structures, with the East China Sea exhibiting the highest concentrations of NO-N, PO-P, and SiO-Si, while the South China Sea showing the lowest levels and a distinct nitrogen-deficient signature.

Efficacy and safety of neoadjuvant toripalimab plus chemotherapy in localized deficient mismatch repair/microsatellite instability-high gastric or esophagogastric junction adenocarcinoma (NICE): a multicentre, single-arm, exploratory phase 2 study.

Background: In locally advanced gastric or gastroesophageal junction adenocarcinoma (GC/EGJC), deficient mismatch repair/microsatellite instability-high (dMMR/MSI-H) tumors exhibit high responsiveness to immunotherapy. The synergistic efficacy of neoadjuvant immunotherapy combined with chemotherapy in dMMR/MSI-H GC/EGJC remains uncertain.

Methods: The NICE trial is a multicentre, single-arm, exploratory phase 2 study conducted at six hospitals in China, evaluating the safety and efficacy of toripalimab in combination with CapeOX as perioperative therapy for locally advanced GC/EGJC across three biomarker-defined cohorts.

The intraoperative hemodynamic and clinical research of three direct bypasses in moyamoya disease a prospective cohort of 98 cases.

The goal of this study was to investigate clinical and hemodynamic differences in moyamoya disease patients underwent direct bypass using one donor-one recipient (1D1R), one donor-two recipients (1D2R), and two donors-two recipients (2D2R) bypass. To the authors' knowledge, this represents the largest series reported to date. Clinical and radiographic data were collected for 98 patients using 1D1R (28 cases), 1D2R (46 cases), and 2D2R (24 cases) bypasses.

Single-cell transcriptomics unveils leukocyte heterogeneity in the gills of in response to parasitic infection.

Background: Fish gills serve as critical immune interfaces against aquatic pathogens, yet their leukocyte heterogeneity in response to parasitic infections remains poorly understood.

Methods: Single-cell RNA sequencing was employed to elucidate leukocyte responses in the gills of during infection.

Results: A total of 13,070 leukocytes from the gills under steady-state and infected conditions were profiled and classified into eight principal lineages: T cells (> 70% of total immune cells), ILC2-like cells, NK-like cells, neutrophils, granulocytes, B cells, macrophages, and dendritic cells.

Regakine-1 homologue regulates leukocyte migration and activation in large yellow croaker (Larimichthys crocea).

Chemokines are essential components of the vertebrate immune system, playing a crucial role in modulating the migration and functional activation of leukocytes. Among the chemokine superfamily, CC chemokines constitute the largest subgroup in mammals. Although this subfamily exhibits a significant expansion in teleosts, its functional roles remain largely unexplored.

Nanosecond pulsed electric field-empowered physical-chemical cascade ferroptosis therapy for triple-negative breast cancer.

Bis-allylic hydrogen atoms of polyunsaturated fatty acids (PUFAs) in biological membranes are major initiation sites of ferroptosis. However, the location of PUFAs in the hydrophobic interior of the lipid bilayer makes it challenging for most ferroptosis-inducing drugs to interact with the PUFAs to promote lipid peroxidation (LPO), potentially limiting the occurrence of ferroptosis. Herein, we propose a new generalized method named physical-chemical cascade ferroptosis (PCCF) for triggering ferroptosis by disrupting the integrity of the cell membrane to expose bis-allylic hydrogen atoms that react with nanozyme MnFI under nanosecond pulsed electric field (nsPEF) assistance.

Functional characterization and epigenetic regulation of Tcf3a and Tcf3b during IgM B cell activation in large yellow croaker (Larimichthys crocea).

The transcription factor Tcf3 is a key regulator during mammalian B cell development and activation, which is highly expressed in precursor B cells, downregulated in mature B cells, and re-expressed upon B cell activation. Despite the extensive studies in mammals, its role in teleost B cells remains poorly characterized. Here, using the economically important marine teleost large yellow croaker (Larimichthys crocea) as a model, we identified two Tcf3 homologs (LcTcf3a and LcTcf3b) and found that both genes were upregulated during B cell activation.

Characterization of CD8 T Cells in large yellow croaker Larimichthys crocea and their dynamics in response to Cryptocaryon irritans infection.

CD8 T cells are critical cytotoxic effectors involved in pathogen clearance, including defense against parasitic infections in aquaculture species. In this study, we developed a monoclonal antibody specifically targeting the CD8α chain, enabling the identification of CD8 T cells in large yellow croaker Larimichthys crocea and providing a valuable tool for studying cytotoxic T cell responses in teleosts. Croaker CD8 T cells were more abundant in mucosal than systemic lymphoid organs, suggesting their preferential localization to sites of pathogen entry.

Identification and bioactivity of a programmed death-ligand 1 homologue in large yellow croaker (Larimichthys crocea).

As a pivotal checkpoint molecule in immune system, programmed death-ligand 1 (PD-L1) orchestrates T cell immunity by suppressing T cell proliferation/activation, promoting apoptosis/anergy/exhaustion, and inducing regulatory T cell differentiation. Although teleosts possess a PD-L1 homologue capable of executing analogous functions, its immunological mechanisms remain largely uncharacterized. Here, we characterized a PD-L1 homologue (LcPD-L1) in a commercially significant species, Larimichthys crocea.

Trade-off strategy under stress: Growth, reproduction, and antioxidant defense in Daphnia magna exposed to 6PPD-Q.

The occurrence of tire-derived chemical N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine quinone (6PPD-Q) has raised concerns about its environmental toxicity. However, the toxicity of 6PPD-Q on aquatic invertebrates remains insufficiently characterized. In this study, a model organism, Daphnia magna, was exposed to 0, 0.

Targeting tumor-associated CCR2 macrophages to inhibit pancreatic cancer recurrence following irreversible electroporation.

Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal malignancy with pronounced resistance to conventional therapies. Irreversible electroporation (IRE) is a promising therapy for PDAC; however, its clinical efficacy is limited by a high recurrence rate. Here, using a preclinical PDAC model, we characterized the tumor immune microenvironment following insufficient IRE (iIRE) through single-cell RNA sequencing.

Phenotypic and functional characterization of CD4-2 T cells in large yellow croaker Larimichthys crocea.

CD4 T cells, also known as helper T (Th) cells, are crucial regulators of the adaptive immune system, orchestrating and coordinating immune responses that protect the host against a broad spectrum of pathogenic threats. Two CD4 co-receptor genes, cd4-1 and cd4-2, have been identified in teleost species, including the large yellow croaker Larimichthys crocea. In this study, we developed a monoclonal antibody (mAb) that specifically recognizes croaker CD4-2, enabling the identification and characterization of CD4-2 T lymphocytes.

Roles of B7 homologue 3 protein (B7-H3) in T cell responses of large yellow croaker (Larimichthys crocea).

The B7 family provides critical co-stimulatory signals for T cell activation through receptor interactions, thereby modulating T cell proliferation and function. However, the roles and downstream signaling pathways of these molecules in teleosts remain largely unexplored. In the present study, we identified a B7-H3 homologue (LcB7-H3) in large yellow croaker (Larimichthys crocea), a commercially significant marine teleost species.

UBE2T-mediated HP1α ubiquitination enhances nucleolar function and promotes the progression of IDH1/TP53-mutant glioma.

Background: High-grade glioma is the most aggressive form of primary brain tumor, characterized by rapid progression and a grim prognosis. The presence of mutations in the IDH1 and TP53 is associated with a specific molecular phenotype in glioma, and their interaction is a potential target for therapy.

Methods: Our study utilized a combination of bioinformatics analysis, in vitro experiments, and in vivo tumor xenograft models to investigate the role of the ubiquitin-conjugating enzyme UBE2T in the malignant progression of IDH1/TP53 mutant glioma.

Progress in targeting the NLRP3 signaling pathway for inflammatory bowel disease (Review).

Inflammatory caspases pro‑inflammatory cytokines, such as IL‑1β IL‑18, constitute a critical part in the modulation of innate immune responses by the nucleotide‑binding domain leucine‑rich repeat protein (NLRP)3 inflammasome. The NLRP3 inflammasome, a cytosolic complex, is composed of three distinct classes of proteins‑sensor complexes, adaptor proteins, cysteine proteases. It is well‑established that this pathway can powerfully influence the pathophysiology of autoimmune diseases along with an association of a plethora of inflammatory conditions.

Transcriptomic analysis reveals the role of MAPK signaling pathway in IgM B cells against Pseudomonas plecoglossicida infection in large yellow croaker (Larimichthys crocea).

The large yellow croaker (Larimichthys crocea), an economically important marine fish in China, often suffers serious losses due to visceral white nodule disease caused by Pseudomonas plecoglossicida infection. IgM B cells play critical roles in the defense against bacterial infection, yet their immune response and underlying mechanisms against the P. plecoglossicida infection in large yellow croaker remain poorly characterized.

Regulation of Intestinal Inflammation in Large Yellow Croaker (Larimichthys crocea) by Soybean Globulin via Toll-Like Receptor Signaling.

Larimichthys crocea, an economically significant fish species, has experienced significant resource depletion due to overfishing, prompting the expansion of aquaculture. However, the utilization of plant-based protein sources, like soybean globulin, in aquafeeds may induce intestinal inflammation, compromising fish health. In this study, models of Toll-like receptor (TLR) gene expression were constructed using dual-luciferase reporter vectors.

Peptides derived from the α-helical structure of large yellow croaker (Larimichthys crocea) CXCL8 demonstrate potent bactericidal effects.

Bacterial diseases pose a significant threat to the large yellow croaker aquaculture industry, and the overuse of antibiotics has exacerbated the issue of bacterial resistance. Consequently, there is an urgent need for antimicrobial peptides as alternatives to traditional antibiotics. In this study, a 13-amino acid peptide, AVV13N, was designed by truncating the carboxyl-terminal region of the large yellow croaker CXCL8 protein and amidating its carboxyl terminus.

Chromosome-level genome assembly of Pinus massoniana provides insights into conifer adaptive evolution.

Pinus massoniana, a conifer of significant economic and ecological value in China, is renowned for its wide adaptability and oleoresin production. We sequenced and assembled the chromosomal-level P. massoniana genome, revealing 80,366 protein-coding genes and significant gene family expansions associated with stress response and plant-pathogen interactions.

Metagenomic analysis reveals distinct patterns of gut microbiota features with diversified functions in infection (CDI), asymptomatic carriage and non-CDI diarrhea.

infection (CDI) has been recognized as a leading cause of healthcare-associated infections and a considerable threat to public health globally. Increasing evidence suggests that the gut microbiota plays a key role in the pathogenesis of CDI. The taxonomic composition and functional capacity of the gut microbiota associated with CDI have not been studied systematically.

Molecular characterization and functional analysis of IL-18 in large yellow croaker (Larimichthys crocea).

Interleukin-18 (IL-18), a pro-inflammatory cytokine of the IL-1 family, is crucial for protecting the host against pathogen infection in mammals. In this study, a IL-18 homolog gene was cloned from large yellow croaker (Larimichthys crocea) (LcIL-18), which has an open reading frame (ORF) of 609 bp that encodes a polypeptide of 202 amino acids. The LcIL-18 C-terminus contains a typical IL-1 family signature and a caspase cleavage site.

Joint Nasogastric Tube Versus Traditional Decompression Nasogastric Tube to Guided OGT-Overlap Esophagojejunostomy in Laparoscopic Total Gastrectomy: A Randomized Controlled Trial.

Background: Our team pioneered the use of a joint nasogastric tube (JNT) for pairing overlap-esophagojejunostomy guiding tube (OGT) in an OGT-overlap esophagojejunostomy, demonstrating its safety and efficacy.

Objective: We aimed to compare the safety and efficacy of a novel technique, the JNT, with traditional nasogastric tubing (TNT) in guiding OGT-overlap esophagojejunostomy.

Methods: From January 2023 to January 2024, a total of 108 gastric/gastroesophageal junction (G/GEJ) cancer patients undergoing laparoscopic total gastrectomy (LTG) were eligible for inclusion in this study; however, 2 patients were excluded as they underwent hyperthermic intraperitoneal chemotherapy (HIPEC) after surgery.