Isolation, characterization and application of probiotic Lactobacillus sakei from Larimichthys crocea intestine.

Currently, disease management is a major challenge for high-density aquaculture in China and a major factor limiting the healthy and sustainable development of our aquaculture industry. Research on fish-derived probiotics as safe feed additives is still relatively limited. In this study, we screened Larimichthys crocea for intestinal lactobacilli, obtained high adhesion strain Lactobacillus sakei YLD10, and further investigated its probiotic function and mechanism of resistance to Pseudomonas plecoglossicida infection.

Regulation of Intestinal Inflammation in Large Yellow Croaker (Larimichthys crocea) by Soybean Globulin via Toll-Like Receptor Signaling.

Larimichthys crocea, an economically significant fish species, has experienced significant resource depletion due to overfishing, prompting the expansion of aquaculture. However, the utilization of plant-based protein sources, like soybean globulin, in aquafeeds may induce intestinal inflammation, compromising fish health. In this study, models of Toll-like receptor (TLR) gene expression were constructed using dual-luciferase reporter vectors.

Eucommia ulmoides leaf extracts combined with Astragalus polysaccharides: Effects on growth, antioxidant capacity, and intestinal inflammation in juvenile large yellow croaker (Larimichthys crocea).

Eucommia ulmoides leaf extract (ELE) and Astragalus polysaccharides (APS) have been widely used as immunopotentiators in aquaculture. Our prior research on large yellow croaker (Larimichthys crocea) demonstrated that dietary 1 g/kg APS bolstered fish immunity and antioxidant defense. However, the combined effect of ELE and APS in juvenile large yellow croaker remains unknown.

A Review on Face Mask Recognition.

This review offers a comprehensive and in-depth analysis of face mask detection and recognition technologies, emphasizing their critical role in both public health and technological advancements. Existing detection methods are systematically categorized into three primary classes: feaRture-extraction-and-classification-based approaches, object-detection-models-based methods and multi-sensor-fusion-based methods. Through a detailed comparison, their respective workflows, strengths, limitations, and applicability across different contexts are examined.

sRNA113 regulates motility to affect immune response against infection in pearl gentian grouper.

Small RNAs (sRNAs) are a class of molecules capable of perceiving environmental changes and exerting post-transcriptional regulation over target gene expression, thereby influencing bacterial virulence and host immune responses. is a pathogenic bacterium that poses a significant threat to aquatic animal health. However, the regulatory mechanisms of sRNAs in .

lafK contributes the regulation of swarming motility of Pseudomonas plecoglossicida and bacterial-host interaction.

Flagella-mediated swarming motility plays a crucial role in facilitating the rapid colonization and dissemination of bacterial within the host. The swarming motility of Pseudomonas plecoglossicida is intricately associated with its lateral flagella, and notably, the lateral flagella system of P. plecoglossicida encompasses a transcriptional regulator known as LafK.

Isolation and identification of probiotic AJQ03 from the intestinal tract of (Japanese eel).

In recent years, the use of fish-derived probiotics in aquaculture has become more widespread. However, research on -derived probiotics is still limited. To evaluate the potential of probiotics for disease control in eel aquaculture, isolates were obtained from the intestinal tract of healthy .

Role of the Pseudomonas plecoglossicida fliL gene in immune response of infected hybrid groupers (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂).

, a gram-negative bacterium, is the main pathogen of visceral white-point disease in marine fish, responsible for substantial economic losses in the aquaculture industry. The FliL protein, involved in torque production of the bacterial flagella motor, is essential for the pathogenicity of a variety of bacteria. In the current study, the gene deletion strain (Δ), gene complement strain (C-Δ), and wild-type strain (NZBD9) were compared to explore the influence of the gene on pathogenicity and its role in host immune response.

Comparative transcriptome analysis explored the molecular mechanisms of a luxR-type regulator regulating intracellular survival of Aeromonas hydrophila.

Aeromonas hydrophila is not a traditional intracellular bacterium. However, previous studies revealed that pathogenic A. hydrophila B11 could temporarily survive for at least 24 h in fish phagocytes, and the regulation of intracellular survival in bacteria was associated with regulators of the LuxR-type.

Pseudomonas plecoglossicida fliP gene affects the immune response of Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂ to infection.

Pseudomonas plecoglossicida is a pathogen that causes visceral white spot disease in a variety of teleosts. The protein encoded by fliP gene is involved in the assembly of bacterial flagella, which plays a vital role in bacterial pathogenicity. However, the roles of the fliP gene on the host immune response remain unclear.

Comparative study on the effects of crystalline L-methionine and methionine hydroxy analogue calcium supplementations in the diet of juvenile Pacific white shrimp ().

An 8-week feeding trial was conducted to evaluate the effects of L-methionine and methionine hydroxy analogue calcium (MHA-Ca) supplements in low-fishmeal diet on growth performance, hepatopancreas morphology, protein metabolism, anti-oxidative capacity, and immunity of Pacific white shrimp ( ). Four isonitrogenous and isoenergetic diets were designed: PC (203.3 g/kg fishmeal), NC (100 g/kg fishmeal), MET (100 g/kg fishmeal +3 g/kg L-methionine) and MHA-Ca (100 g/kg fishmeal +3 g/kg MHA-Ca).

Function of the rpoD gene in Pseudomonas plecoglossicida pathogenicity and Epinephelus coioides immune response.

Pseudomonas plecoglossicida is a Gram-negative pathogenic bacterium that causes visceral white spot disease in several marine fish species, resulting in high mortality and financial loss. Based on previous RNA sequencing (RNA-seq) results, rpoD gene expression is significantly up-regulated in P. plecoglossicida during infection, indicating that rpoD may contribute to bacterial pathogenicity.

The contribution of exbB gene to pathogenicity of Pseudomonas plecoglossicida and its interactions with Epinephelus coioides.

To study the roles of the exbB gene in Pseudomonas plecoglossicida during interactions with Epinephelus coioides, five short hairpin RNAs (shRNAs) were designed and synthesized to silence the exbB gene in P. plecoglossicida which resulted in significant reductions in exbB mRNA expression. The mutant with the best silencing efficiency (89.

The contributions of fliG gene to the pathogenicity of Pseudomonas plecoglossicida and pathogen-host interactions with Epinephelus coioides.

Pseudomonas plecoglossicida is a Gram-negative aerobic rod-shaped bacterium with polar flagella. It is the causative agent of visceral white spot disease in cultured fish, resulting in serious economic losses. In our previous study, RNA sequencing showed that the expression of the fliG gene in P.

The Effect of Gene on the Virulence of and the Immune Response of .

is the causative agent of "visceral white spot disease" in cultured fish and has resulted in serious economic losses. gene plays a crucial role in the uptake of nutrients from the outer membranes in Gram-negative bacteria. The previous results of our lab showed that the expression of gene of was significantly upregulated in the spleens of infected .

Integration of RNA-seq and RNAi provides a novel insight into the immune responses of Epinephelus coioides to the impB gene of Pseudomonas plecoglossicida.

Pseudomonas plecoglossicida is a Gram-negative bacterium that causes visceral white spot disease in Epinephelus coioides and leads to severe aquatic economic losses. The RNA-seq results of a previous study showed that the expression of the impB gene in P. plecoglossicida was significantly upregulated during infection.

Aryl hydrocarbon receptor is required for immune response in Epinephelus coioides and Danio rerio infected by Pseudomonas plecoglossicida.

Aryl hydrocarbon receptor (AhR), a ligand-dependent transcriptional factor that responds to environmental chemicals, has been recently found to be closely associated with immune response in mammals. Pseudomonas plecoglossicida (P. plecoglossicida) is a temperature-dependent bacterial pathogen of visceral white spot disease in fish.

Mechanisms underlying the virulence regulation of new ncRNA Vvrr1 with a comparative proteomic analysis.

The incidence of infections has increased in recent years due to the influence of climate change and rising sea temperature. Vibrio virulence regulatory RNA 1 (Vvrr1) is a newly found noncoding RNA (ncRNA) predicted to be closely related to the adhesion ability of based on the previous RNA-seq. In this study, the target genes of Vvrr1 were fully screened and verified by constructing Vvrr1-overexpressing strains and using the proteome sequencing technology.

Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida Gene in Pathogen-Host Interaction with .

In the present study, and Pseudomonas plecoglossicida were selected as a host-pathogen interaction model for teleosts and prokaryotic pathogens. Five shRNAs were designed and synthesized to silence the gene, all of which resulted in pronounced reductions in mRNA; the mutant strain with the best silencing efficiency of 92.16% was chosen for subsequent analysis.

Dual RNA-seq uncovers the immune response of Larimichthys crocea to the secY gene of Pseudomonas plecoglossicida from the perspective of host-pathogen interactions.

Pseudomonas plecoglossicida is a Gram-negative aerobic bacterium that causes high mortality and serious economic losses in some commercial marine fish. Expression of secY was found to be significantly upregulated at 18 °C compared to 28 °C by RNA-seq and qRT-PCR. All five tested recombinant vectors (pCM130/tac + shRNA) significantly reduced secY mRNA levels in P.

Acinetobacter piscicola sp. nov., isolated from diseased farmed Murray cod (Maccullochella peelii peelii).

A taxonomic study was carried out on strain LW15, which was isolated from the external lesions of diseased farmed Murray cod (Maccullochella peelii peelii) from an intensive culture pond. Cells of strain LW15 were Gram-negative, facultative-anaerobic, non-motile, and both coccobacillus- and bacillus-shaped. Growth was observed at NaCl concentrations of 0-2 % (w/v) (optimum, 0 %), 4-32 °C (optimum, 25-28 °C) and pH 5.