Development and performance optimization of a portable Gas Chromatography - Photoionization Detection system for trace electrolyte leakage detection in lithium batteries.

With the widespread application of lithium batteries in energy storage systems, their safety concerns have attracted increasing attention. Electrolyte leakage, as one of the primary safety hazards, necessitates highly sensitive and rapid detection technologies for early warning. Addressing the limitations of conventional methods (e.

Nascent liver proteome reveals enzymes and transcription regulators under physiological and alcohol exposure conditions.

The liver proteome undergoes dynamic changes while performing hundreds of essential biological functions. Dysregulation of the liver proteome under alcoholic conditions leads to alcohol-associated liver disease (ALD), a major health challenge worldwide. There is an urgent need for quantitative and liver-specific proteome information in living animals to understand the pathophysiological dynamics of this largest solid organ.

Chronic ISG15 Exposure Accelerates CD8+ T-cell Dysfunction while Increasing PD-1 Blockade Sensitivity in Oral Squamous Cell Carcinoma.

Immunotherapy has emerged as a promising treatment for head and neck squamous cell carcinoma (HNSCC), yet clinical responses remain limited. Elevated expression of interferon-stimulated gene 15 (ISG15), commonly observed in oral squamous cell carcinoma (OSCC), may contribute to this limited efficacy. Although chronic interferon signaling is known to impair CD8+ T cell function, the specific role of secreted ISG15 in T cell exhaustion remains unclear.

Spectroscopic evidence of symmetry breaking in the superconducting vortices of UTe.

The recently discovered heavy-fermion superconductor, UTe, is an excellent candidate for spin-triplet superconductors in which electrons form spin-triplet Cooper pairs with spin  = 1 and odd parity. Unconventional superconductivity often hosts unconventional vortices. Yet, the vortex core and lattice in UTe have not been directly visualized and characterized.

Method for identifying the locations of longitudinal seams based on LiDAR data detected inside shield tunnel.

After extracting the longitudinal seam (LS) LiDAR detected data inside shield tunnel, although most can be obtained, some are obscured by tunnel objects and scanning limitations . Additionally, some segment point clouds are misidentified as seams, leading to extraction inaccuracies. Therefore, this study proposes an inference method based on geometric information to identify the extracted LS point clouds to estimate the longitudinal seams' locations that could not be extracted.

Accurate Identification of Cell Cycle Stages in RPE1 Cells Using the ImmunoCellCycle-ID Method.

Accurate identification of cell cycle stages is essential for investigating fundamental biological processes such as proliferation, differentiation, and tumorigenesis. While flow cytometry remains a widely used technique for such analyses, it is limited by its lack of single-cell resolution and its requirement for large sample sizes due to its population-based approach. These limitations underscore the need for alternative or complementary methods that offer single-cell precision with compatibility for small-scale applications.

Efficient derivation of stable sheep embryonic stem cells opens a new avenue for agricultural and biomedical application.

Introduction: Embryonic stem cells (ESCs), capable of generating all adult cell types, hold transformative potential for agriculture and biomedicine. However, stable sheep ESCs derivation remains challenging, limiting their application in further research.

Objectives: We aimed to establish stable sheep ESCs using a simplified protocol, validate their pluripotency, and demonstrate genome editing utility.

Overcoming the Diagnostic Challenges in Cutaneous Cryptococcosis Caused by Cryptococcus neoformans: A Case Study Utilizing Metagenomic Next-Generation Sequencing.

Cryptococcosis, a rare deep fungal infection, primarily affects immunocompromised individuals and carries a high mortality risk when hematogenously disseminated to the central nervous system (CNS). Cutaneous cryptococcosis poses diagnostic challenges due to its diverse and nonspecific dermatological manifestations. Traditional diagnostic methods, including mycological examination, culture, and histopathology, are time-consuming and require specialized expertise, often leading to misdiagnosis.

TLR7/8 Agonist R848 Regulates X Sperm Energy Metabolism in Dairy Cows via Inhibiting Glycolysis and Fatty Acid β-Oxidation by LKB1/AMPK Pathway.

Sex control is an efficient biotechnology for improving animal reproductive efficiency which can significantly improve the economic benefits of dairy industry. Flow cytometry is a common method for sex control in dairy cows and can effectively sort X/Y sperm, but its equipment is expensive. Recent studies have shown a new inexpensive potential method that utilizing R848 (a TLR7/8 agonist) to effectively stratify mammalian X/Y sperm by activating X sperm specific proteins.

Research on the dynamic pressure influence and support engineering strategy of roadway group under multi section collaborative mining conditions.

Multiple roadways have been excavated to form an underground production system, which is used for multi section collaborative mining of room pillar structures, achieving efficient mining of underground inclined and steeply inclined ore bodies; however, due to the combined influence of surrounding rock stress disturbance in the roadway group and lateral stress of the ore room, the dynamic pressure influence of roadway group is prominent in multi section collaborative mining. In this article, a combination of various research methods, such as engineering research, numerical simulation, theoretical analysis, and engineering practice, are adopted with the multi section collaborative mining roadway group in Xifeng Mine as the engineering background; the research work has been carried out on the impact of dynamic pressure manifestation on roadway groups under multi section collaborative mining conditions, the mechanism of dynamic pressure roadway failure and instability, roadway support control strategies, and the design of dynamic pressure roadway group support in Xifeng Mine. Research has shown that: (1) for multi section collaborative mining engineering conditions, a reasonable design of interlayer distance is the key to the surrounding rock stability of the dynamic pressure roadway group, which can reduce the dynamic pressure impact of each middle section roadway on each other, while meeting the mining technology level and efficiency requirements of the ore body; (2) the end of the ore vein mining roadway near the ore room is a key support reinforcement area, which increases the risk of instability of the surrounding rock due to strong mining induced pressure; the secondary support and reinforcement area is the intersection of the ore vein mining roadway and external roadway, as well as the roof of the ore vein external roadway and the side walls near the ore room, which are affected by the risk of shallow loose body collapse; (3) The layout of the roadway group causes uneven disturbance of the surrounding rock stress environment, changes the stable bearing state, and thus intensifies the damage; The mining of the ore room causes the surrounding stress field to be relieved, and the stress imbalance and disturbance of the roadway surrounding rock are more obvious, leading to an increase in the hidden danger of failure and instability; (4) A joint support scheme for dynamic pressure roadway group was proposed (active support with "anchor rod + steel mesh + surface spraying" during the layout stage of the roadway group + passive reinforcement support with "steel arch + metal mesh + surface spraying" during the ore drawing stage), and the on-site support effect was safe and stable.

Dextrose prolotherapy at varying concentrations ameliorates tendon injury via IGF-2R: an integrated study of Mendelian randomization and an animal model.

Background: This study integrates Mendelian randomization (MR) analysis with animal experiments to systematically evaluate the therapeutic efficacy of varying concentrations of hypertonic dextrose on tendon injury and elucidate the underlying mechanisms in the context of tendon repair.

Methods: The SwissTargetPrediction database was utilized to identify potential molecular targets of dextrose. Two-sample MR analysis was performed to investigate the causal relationships between the identified targets and Achilles tendinopathy.

CARM1-Mediated MAP2K4 Methylation Potentiates the Oncogenic Functions of MAP2K4 and Constitutes a Targetable Dependency in Triple-Negative Breast Cancer.

Unlabelled: The arginine methyltransferase coactivator-associated arginine methyltransferase 1 (CARM1) is amplified/overexpressed in a variety of cancers, including triple-negative breast cancer (TNBC), spurring the interest of developing CARM1 inhibitors (CARM1i). In this study, we discovered that CARM1i treatment leads to elevated CARM1 levels as well as activation of AKT, which could result in long-term treatment resistance in breast cancer cells. CARM1 methylated MAP2K4 at arginine 58 in TNBC, and methylated-MAP2K4 localized to the nucleus and potentiated the proliferation- and metastasis-promoting functions of MAP2K4 via activation of JNK signaling.

BRD4 regulates PAI-1 expression in tumor-associated macrophages to drive chemoresistance in colorectal cancer.

Tumor-associated macrophages (TAMs) in the tumor microenvironment play a key role in drug resistance, but the mechanisms underlying TAM polarization and its role in drug resistance remain unclear. Here, we identified BRD4 as a critical factor in TAM polarization and drug resistance in colorectal cancer (CRC). BRD4 deficiency in macrophages impaired M2-like TAM polarization, and tumors from myeloid-lineage specific Brd4 conditional knockout (Brd4-CKO) mice displayed a reduction in infiltrating M2-like TAMs and an enhanced anti-tumor microenvironment.

Engineering eukaryotic transposon-encoded Fanzor2 system for genome editing in mammals.

Eukaryotic transposon-encoded Fanzor proteins hold great promise for genome-engineering applications as a result of their compact size and mechanistic resemblance to TnpB. However, the unmodified Fanzor systems show extremely low activity in mammalian cells. Guided by the predicted structure of a Fanzor2 complex using AlphaFold3, we engineered the NlovFz2 nuclease and its cognate ωRNA to create an evolved enNlovFz2 system, with an expanded target-adjacent motif (TAM) recognition scope (5'-NMYG) and a substantially improved genome-editing efficiency, achieving an 11.

Secondary hemophagocytic lymphohistiocytosis in a 5-month-old infant with IBD post-COVID-19: a case report.

Background: COVID-19 is known to induce cytokine storms and inappropriate cytotoxic immune responses. Hemophagocytic lymphohistiocytosis (HLH) is an underrecognized condition due to a hyperinflammatory syndrome characterized by fulminant hypercytokinemia with a high mortality burden. Cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induced secondary HLH during and post-infection have been sparsely reported in children.

Preimplantation genetic testing for type 2 diabetes based on family-specific polygenic risk score: A proof-of-concept study.

Aims: This study aims to evaluate the feasibility of family-specific polygenic risk prediction in reducing the risk of type 2 diabetes (T2D) in the offspring from an infertile couple with a family history of early-onset T2D.

Methods: We innovatively established a family-specific polygenic risk prediction model for this T2D family and the embryo with the lowest risk of T2D were selected for implantation.

Results: Initially, whole exome sequencing analysis in the family failed to identify monogenic-level pathogenic or likely pathogenic variants responsible for T2D.

Metagenomic analysis reveals distinct patterns of gut microbiota features with diversified functions in infection (CDI), asymptomatic carriage and non-CDI diarrhea.

infection (CDI) has been recognized as a leading cause of healthcare-associated infections and a considerable threat to public health globally. Increasing evidence suggests that the gut microbiota plays a key role in the pathogenesis of CDI. The taxonomic composition and functional capacity of the gut microbiota associated with CDI have not been studied systematically.

Varicose vein in patients under the age of 40: A multicenter case-control study.

ObjectiveTo assess the risk factors for varicose veins in patients under the age of 40 and the long-term results of surgery.MethodsThis multicenter case-control study comprised patients who received great saphenous vein stripping and ambulatory phlebectomy between January 2014 and December 2015 and were followed for at least 5 years. Patients under the age of 40 were assigned to Group 1.

Impact of Sagittal Spinal Balance on Functional Recovery After PLIF of L5-S1 Isthmic Spondylolisthesis With Lumbar Spinal Stenosis.

Objective: Despite the widespread use of posterior lumbar interbody fusion (PLIF) for L5-S1 isthmic spondylolisthesis (IS) with lumbar spinal stenosis (LSS), residual sagittal imbalance critically impairs long-term pain relief and functional recovery. This study analyzes the influence of residual sagittal imbalanceon health-related quality of life (HRQOL) after PLIF for L5-S1 IS with LSS, aiming to optimize surgical correction and prognostic accuracy.

Methods: This study analyzed 103 consecutive patients with L5-S1 IS and LSS undergoing PLIF from 2020 to 2022, followed at 3 days, 3 months, and ≥ 1 year postoperatively, using SPSS 26.

Applications of Multicolor Light-Emitting Devices Fabricated Using Tricolor Plastic Scintillators in LED Lighting, X-ray Detection, and Imaging.

Plastic scintillators made of rare-earth complexs and organic scintillators are widely applied in radiation detection, environmental protection, nuclear medicine and industrial applications. Here, we fabricate the multicolor light-emitting devices by precisely controlling the composition and ratio of three scintillating materials doped into PMMA films using rare-earth complexes and organic compounds as dopants. Notably, the dose detection limits for the 'R' and 'RB' scintillating films are measured to be 2.

Establishment and application of an RNA-seq-based predictive model for the hourly precision window of implantation (WOI) in patients with recurrent implantation failure.

Purpose: This study aims to establish a single-time point RNA-sequencing-based endometrial receptivity test (rsERT) to provide hourly precision of endometrial WOI for patients with recurrent implantation failure (RIF).

Methods: A two-phase study was conducted. In the first phase, a total of 70 patients with successful intrauterine pregnancy after personalized embryo transfer (pET) guided by three-time points rsERT were recruited for modified rsERT.

High-Precision 2D-DOA Estimation Method for Millimeter-Wave Radar Based on Double-Parallel Linear Array and Joint IAA-RIT.

High-precision two-dimensional direction of arrival (2D-DOA) estimation is an important mean of millimeter-wave radar for accurate target location. Aiming at the problems such as limited antenna aperture, signal coherence, and a few snapshots in millimeter-wave radar target detection, this paper proposes a 2D-DOA estimation method by using a joint iterative adaptive approach and rotational invariance technique (IAA-RIT) based on the double-parallel linear array. This method first constructs an iterative adaptive approach spectrum based on subarray 1 in the double-parallel linear array and then calculates the coupling angle estimate with the azimuth and the elevation.

Extracorporeal membrane oxygenation in the treatment of critical Pneumocystis jirovecii pneumonia in a child with Langerhans cell histiocytosis: a case report and literature review.

Background: Children with Langerhans cell histiocytosis (LCH) are particularly susceptible to infections such as Pneumocystis jirovecii pneumonia (PJP) due to the immunosuppressive effects of chemotherapy, which can progress to acute respiratory distress syndrome (ARDS) and respiratory failure. The use of Extracorporeal Membrane Oxygenation (ECMO) to manage hypoxemia secondary to PJP in LCH presents unique challenges, including the prevention of catheter-related bloodstream infections associated with arterial and venous access. This study explores a case wherein ECMO was crucial in treating severe PJP-induced respiratory failure in a pediatric patient with LCH.

Novel minimally invasive carpal tunnel release using a specialized surgical kit: a prospective multi-center case series.

Background: Carpal tunnel syndrome (CTS) is the most common peripheral nerve compression neuropathy. While established surgical techniques have demonstrated reliable outcomes and safety profiles, innovations in minimally invasive approached continue to emerge. This study evaluates a novel minimally invasive surgical technique using a specialized instrument for carpal tunnel release.

Clinical utility of trio whole exome sequencing in fetuses with ultrasound anomalies.

Introduction: Ultrasound scanning anomalies in fetuses are a cause for concern and often necessitate further diagnostic procedures. This retrospective study evaluated the utility of trio whole exome sequencing (trio-WES) in the diagnosis of fetuses with ultrasound anomalies.

Methods: We included fetuses diagnosed with fetal ultrasound anomalies referred to the First Affiliated Hospital of Chongqing Medical University between November 2018 and July 2023.