NOTCH signaling orchestrates the inflammatory-fibrotic continuum of macrophages in renal allograft rejection.

Background: Chronic rejection is a major cause of long-term kidney allograft failure, characterized by persistent inflammation and progressive fibrosis. Macrophages are central mediators of this process, but their phenotypic heterogeneity and regulatory mechanisms in chronic rejection remain incompletely understood.

Methods: We performed single-cell transcriptomic analysis on renal allograft biopsies from patients with different types of rejection and on a time-course rat model of chronic rejection.

In vitro activity of Eravacycline against carbapenem-resistant gram-negative bacilli and associated risk factors for non-susceptible infections from a tertiary hospital in fujian, China from 2021 to 2024.

Background: This study evaluated Eravacycline (ERV)'s effectiveness against carbapenem-resistant gram-negative bacteria (CRGNB) and identified risk factors for ERV non-susceptible Klebsiella pneumoniae (ENSKP) infections to support clinical treatment and early detection.

Methods: Between 2021 and 2024, 235 Carbapenem-Resistant Acinetobacter baumannii(CRAB) strains, 48 Carbapenem-Resistant Escherichia coli (CRECO) strains, and 158 Klebsiella pneumoniae (KP) strains were collected. Resistance genes were identified using PCR, and the minimum inhibitory concentration of tigecycline and ERV was determined using the broth microdilution method.

Induced sputum: current progress and prospect.

Induced sputum is a noninvasive, safe, cost-effective, and reproducible method that is particularly advantageous for individuals who do not naturally produce sputum or provide inadequate samples. Its higher quality than spontaneously produced sputum makes it valuable in various respiratory conditions such as chronic obstructive pulmonary disease, asthma, lung cancer, and infectious respiratory diseases. Despite its potential, the clinical use of induced sputum is limited.

Efficacy of Dexmedetomidine as an Adjuvant to Ropivacaine for Intercostal Nerve Block in Elderly Patients Undergoing Video-Assisted Thoracoscopic Esophagectomy: A randomized Double-Blinded Trial.

Background: Esophagectomy is associated with significant postoperative pain and a pronounced perioperative stress response. Dexmedetomidine (DEX) has been widely recognized as an effective adjuvant to regional anesthesia across various surgical procedures. However, its efficacy and safety as an adjuvant to ropivacaine in elderly patients undergoing thoracoscopic esophagectomy remain unclear.

Association of ABCB1 G2677T polymorphism with atorvastatin lipid efficacy and extended prognosis in patients with cerebral ischemic stroke.

To investigate the association between ABCB1 (2677T > G) (rs2032582) polymorphism and lipid-lowering efficacy of atorvastatin with long-term prognosis in patients with cerebral ischemic stroke (CIS). A prospective cohort of 127 CIS patients admitted to Xuchang Central Hospital from January to December 2021 was consecutively enrolled. ABCB1 (2677T > G) genotypes were determined using digital fluorescence molecular hybridization technology, categorizing patients into GG (n = 48), GT (n = 49), and TT (n = 30) groups.

An optimized SPE-UPLC-MS/MS method for simultaneous quantification of 11 tetracyclines in dairy products.

Background: The global rise in antibiotic use, particularly tetracyclines (TCs) in animal husbandry, poses significant risks to food safety and public health due to residual accumulation and bacterial resistance. Existing detection methods for TCs in dairy products often lack efficiency, sensitivity, or the capability for simultaneous multi-residue analysis. Developing a rapid, precise, and cost-effective method to monitor TCs in compliance with international maximum residue limits remains an urgent need.

Case Report: Child with Menkes syndrome complicated by bladder diverticula.

Background: Menkes syndrome is a rare X-linked genetic disorder of copper metabolism caused by variants in the gene. It is characterized by developmental delay, hair abnormalities, hypotonia, and organ dysfunction. Bladder diverticula are a rare but recognized urological complication, and its rupture can lead to severe clinical consequences.

Dihydrotanshinone I Targets PGAM1 to Induce SYVN1-Mediated Ubiquitination and Suppress Glycolysis in Hepatocellular Carcinoma.

Phosphoglycerate mutase 1 (PGAM1) is a glycolytic enzyme frequently overexpressed in hepatocellular carcinoma (HCC), contributing to tumor progression through aberrant glycolysis. Dihydrotanshinone I (DHT), a bioactive natural compound derived from Salvia miltiorrhiza , has been proposed as a potential therapeutic agent for HCC. This study aims to characterize DHT as a PGAM1-targeting agent and investigate its anti-HCC effects.

The CCR5/ROS/NRF2 axis Mediates Dihydrotanshinone I-Induced oxidative stress and apoptosis in hepatocellular carcinoma.

Despite therapeutic advances, hepatocellular carcinoma (HCC) remains a leading cause of cancer mortality worldwide. While dihydrotanshinone I (DHT) shows promising anticancer potential, its molecular mechanisms in HCC remain unexplored. Here, we unveil a novel chemokine (C-C motif) receptor 5 (CCR5)-mediated regulatory network targeted by DHT in HCC treatment.

Distributions of pathogenic bacteria, antibiotic resistance genes, and virulence factors in pig farms in China.

The abundance of antibiotic resistance genes (ARGs) in pig feces can lead to their dissemination in the pig farm environment, posing a serious risk to human health through potential exposure and transmission. However, the extent of microbial contamination in pig farms, including ARGs, virulence factor genes (VFGs), mobile genetic elements (MGEs), and human bacterial pathogens (HBPs), is still largely unknown. In this study, metagenomics was employed to identify the composition and characteristics of microorganism communities, ARGs, VFGs, MGEs and HBPs in pig farm environments from seven different regions of China.

Functional Requirement of Niacinamide for Blood Profiles, Antioxidant Status, and Intestinal Health in Finishing Pigs Fed a Low-Protein Diet.

This study investigated the effects of dietary niacinamide supplementation on blood parameters, antioxidant status, and intestinal health in finishing pigs fed low-protein diets. Sixty-four pigs (Duroc × Landrace × Yorkshire; 80.4 ± 0.

The effect of esketamine on emergence delirium in pediatric patients undergoing general anesthesia: a meta-analysis of randomized controlled trials.

Background: The aim of this study was to investigate the effect of esketamine on emergence delirium in pediatric patients.

Methods: We searched Pubmed, Cochrane Controlled Register of Trials, and Embase from inception to December 2024. Studies were independently evaluated for inclusion criteria and exclusion criteria by two reviewers.

A Study of Skeletal Stem Cell Dynamics and Its Potential Applications in the Design of a Titanium Implant for Senile Osteoporosis.

Traditional biomaterial design often prioritizes empirical knowledge over disease mechanisms and pathological dynamics, resulting in imprecise solutions in complex clinical conditions. Age-related osteoporosis (A-OP) is a disease associated with aging, characterized by a dysfunctional pathological microenvironment that hinders the osseointegration of conventional titanium implants. To develop a targeted titanium implant for A-OP, rat single-cell transcriptomics is integrated with human serum-derived transcriptome data to investigate dynamic changes in skeletal stem cells (SSCs) during aging, which guided the implant design.

Dextran-based prodrug-nanoassemblies responsively releasing pidotimod and camptothecin for anti-breast cancer.

Objective: The aim of this study was to develop a pH/GSH-responsive dextran-based drug delivery system, co-delivering camptothecin (CPT) and pidotimod (PTD), to increase anti-breast cancer efficacy.

Methods: CPT was grafted onto dextran disulfide bond, and PTD were concurrently conjugated into dextran by the instrumentality of ester bond. The synthesized conjugate could self-assemble to form prodrug-nanoassemblies (CPT-SS-DEX-PTD NPs) in solution.

Jatrorrhizine Alleviates Calcific Aortic Valve Disease via Interfering With Glycolysis Targeting ALDOA K42 Lactylation.

Calcific aortic valve disease (CAVD) is a common cardiovascular disorder. It is characterized by the thickening, fibrosis, and mineralization of aortic valve leaflets. These pathological changes can progress to heart failure if not managed effectively.

Identification of PRKCQ-AS1 as a Keratinocyte-Derived Exosomal lncRNA That Promotes Th17 Differentiation and IL-17 secretion in Psoriasis Through Bioinformatics, Machine Learning Algorithms, and Cell Experiments.

Background: Psoriasis is an immune-mediated skin disease where Th17 cell differentiation and IL-17 secretion play critical roles. This study investigates key exosomal ncRNAs regulating the Th17/IL-17 axis in psoriasis and their mechanisms.

Methods: We integrated bulk RNA sequencing datasets from the GEO database to construct and evaluate exosome-related patterns.

Engineering eukaryotic transposon-encoded Fanzor2 system for genome editing in mammals.

Eukaryotic transposon-encoded Fanzor proteins hold great promise for genome-engineering applications as a result of their compact size and mechanistic resemblance to TnpB. However, the unmodified Fanzor systems show extremely low activity in mammalian cells. Guided by the predicted structure of a Fanzor2 complex using AlphaFold3, we engineered the NlovFz2 nuclease and its cognate ωRNA to create an evolved enNlovFz2 system, with an expanded target-adjacent motif (TAM) recognition scope (5'-NMYG) and a substantially improved genome-editing efficiency, achieving an 11.

Hybrid Active Sites in Coordination Polymers Enable Ampere-Level Acetylene Semihydrogenation in Membrane Electrode Assembly Systems.

Electrocatalytic acetylene semihydrogenation in membrane electrode assembly systems promises a sustainable pathway for ethylene production, yet faces challenges in catalyst performance and durability. Herein, we developed a Cu coordination polymer with hybrid sites that synergistically integrate open Cu sites and N-heterocyclic carbenes. These hybrid sites bestow the coordination polymer with acetylene gasophilicity, hydrophobicity toward water, and readily accessible active Cu sites, which energetically facilitate acetylene absorption and vinyl intermediate formation, thereby enabling efficient ethylene production at ampere-level current densities.

Grain Growth Kinetics of a Nickel-Based Superalloy Under Electric Pulse Treatment.

Grain boundaries play a vital role in determining the mechanical and physical properties of metallic materials. Heat treatment (HT) is widely employed to modify the content and distribution of grain boundaries. However, achieving precise control by HT remains challenging due to the scale mismatch between heat transfer and microstructure evolution.

Integrated Multi-Tissue Lipidomics and Transcriptomics Reveal Differences in Lipid Composition Between Mashen and Duroc × (Landrace × Yorkshire) Pigs.

Chinese native pig breeds exhibit unique advantages over Western pig breeds, but the specific lipid metabolism mechanisms remain unclear. The phenotypic characteristics of Mashen (MS) pigs and Duroc × (Landrace × Yorkshire) (DLY) pigs are studied. The results show that MS pigs exhibit higher intramuscular fat (IMF) content.

Dietary Enhances Sow Lactation and Piglet Development Through Gut Microbiota and Metabolism.

Piglets weaned per sow per year (PSY) is a crucial metric for assessing the reproductive performances of sows and directly affects both sow productivity and the economic profitability of pig farms [...

Confirmation of enrofloxacin, ofloxacin, sarafloxacin and danofloxacin residues in poultry meat and pork via LLE-SPE-GC-MS/MS coupled with derivatization treatment.

A gas chromatography-tandem mass technique for simultaneous determination of four fluoroquinolone residues in poultry meat and pork was perfect. Trimethylsilyl diazomethane was applied as the derivatizing agent coupled with liquid-liquid and solid-phase extraction. Full scan mass spectrometry and automated selected reaction monitoring were applied for qualitative and quantitative analysis.

Mechanism study on the removal of Cd and acetamiprid from wastewater treatment plant effluent by PMS activated by tobacco stem biochar under humic acid induction.

In this study, tobacco stem based porous biochar was prepared using a direct induced etching process (HA@TSB). The microstructure with the target functional group was confirmed through detailed SEM, FTIR, XPS, and EPR analysis. Combined with PMS for Cd(II) adsorption and acetamiprid (Ace) degradation, experiments showed that under optimal conditions, the removal rate of Cd(II) within 30 minutes was 95.

Molecular mechanistic exploration of conformational shifts induced by class IV anti-RBD antibody IY2A.

The SARS-CoV-2 virus mutates rapidly, reducing the effectiveness of antibodies. The novel Class IV antibody IY2A partially unfolds the receptor-binding domain (RBD), allowing tolerance of antigenic variations and effectively neutralizing Omicron variants. In this study, we used molecular dynamics simulations and alanine scanning combined with interaction entropy method to elucidate how IY2A maintains its binding affinity across Omicron variants.