Fxyd5 downregulation protects the heart from ischemia/reperfusion injury by suppressing myocardial inflammation.

Background: Myocardial ischemia/reperfusion (I/R) injury is a common cause of death. FXYD domain-containing ion transport regulator-5 (Fxyd5) is a type I membrane protein that plays a significant role in mediating cellular functions. However, the expression and function of Fxyd5 in myocardial I/R injury remain unclear.

Antigenic and structural insights into a Henipavirus attachment glycoprotein.

The novel henipavirus, Langya henipavirus (LayV), was identified in China in 2022. The invasion of host cells by the henipavirus is facilitated through the interaction between viral attachment (G) and fusion (F) glycoproteins with receptors on the cell surface. The G proteins of LayV and Mojiang virus (MojV) exhibit high amino acid homology (86%), while they are located in a unique evolutionary clade within the Henipavirus genus.

Systematic analysis identifies CDKN2A as a prognostic biomarker for hepatocellular carcinoma.

Background: Hepatocellular carcinoma (HCC) ranks as the fifth most prevalent malignancy worldwide. Disruptions in copper homeostasis adversely affect liver function. Cuproptosis, a recently defined form of regulated cell death triggered by intracellular copper accumulation, disrupts the tricarboxylic acid cycle and mitochondrial respiration.

Single amino acid substitution in Hendra virus attachment glycoprotein induces cross-neutralizing antibodies against Nipah virus.

Nipah virus (NiV) and Hendra virus (HeV) are highly pathogenic henipaviruses within the Paramyxoviridae family, causing severe respiratory and neurological diseases in humans and animals with fatality rates up to 75%, and no licensed human vaccines or therapeutics. In this study, we identified a unique vulnerable epitope on the NiV attachment glycoprotein (G) recognized by the potent neutralizing antibody 14F8, which targets a receptor-binding site and neutralizes NiV effectively. Using the 2.

Mutations in BrRBM25 encoding an RNA binding motif protein cause premature bolting in Chinese cabbage.

Bolting is an important agronomic character, and premature bolting leads to insufficient leafy head, which further affects the yield and quality of Chinese cabbage. In this study, two allelic early-bolting mutants ebm11-1 and ebm11-2 were isolated from a Chinese cabbage DH line 'FT' by EMS-mutagenesis. Genetic analysis showed that a single recessive nuclear gene controlled the mutant traits of these two mutants.

[Colonoscopy in infants: procedure and disease spectrum analysis of 184 cases].

Objectives: To investigate the underlying causes and clinical manifestations in infants undergoing colonoscopy, and to analyze changes in disease spectrum.

Methods: Clinical data from 180 infants who underwent a total of 184 colonoscopies at the Department of Gastroenterology, Children's Hospital Affiliated to Shandong University from January 2015 to December 2024 were retrospectively analyzed. Patients were grouped by age: ≤6 months (=41) and >6-12 months (=139); and by examination period: 2015-2019 (=83) and 2020-2024 (=97).

Gradient hydrogel with bioactive glass for tendon-bone interface regeneration: Enhancing biomechanical strength and synchronized tissue regeneration.

The interface between tendon and bone is characterized by a gradient multi-tissue structure in a small-sized, localized region, and the tendon insertion cannot fully regenerate following repair for its rupture. Therefore, tendon-bone healing remains a significant challenge in the field of sports medicine. This study aims to design and fabricate a bioactive hydrogel with a continuous ion concentration gradient, using bioactive glass (BG), modified alginate (AlgMA), and gelatin.

Dynamic monitoring of antibody titers in people living with HIV during Omicron epidemic: comparison between unvaccinated and vaccinated individuals.

Background: The 2019 Coronavirus disease (COVID-19) pandemic has affected hundreds of millions globally, resulting in millions of deaths. People living with HIV (PLWH) typically exhibit significantly lower antibody titers after vaccination compared to healthy individuals, and are particularly vulnerable to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) due to their compromised immune system. Given the continuous emergence of SARS-CoV-2 variants, monitoring immune responses in this high-risk population is crucial.

Time-course transcriptome and proteomic dynamics during the de novo shoot organogenesis in Chinese fir (Cunninghamia lanceolata).

De novo shoot organogenesis (DNSO) enables plants to regenerate shoots from various explants, offering valuable opportunities for research and plant biotechnology applications. While significant progress has been made in understanding regeneration in angiosperms, the regulatory mechanisms in gymnosperms, particularly Chinese fir (Cunninghamia lanceolata), remain poorly understood, despite its importance as a key timber species in China. This study successfully established an efficient DNSO protocol for Chinese fir, identifying six distinct stages in the process through cellular-level analysis.

An optimized SPE-UPLC-MS/MS method for simultaneous quantification of 11 tetracyclines in dairy products.

Background: The global rise in antibiotic use, particularly tetracyclines (TCs) in animal husbandry, poses significant risks to food safety and public health due to residual accumulation and bacterial resistance. Existing detection methods for TCs in dairy products often lack efficiency, sensitivity, or the capability for simultaneous multi-residue analysis. Developing a rapid, precise, and cost-effective method to monitor TCs in compliance with international maximum residue limits remains an urgent need.

Asymmetric Coordinated Single-Atom Catalysts Offering Zero-Order Sulfur Redox Kinetics for High Performance Li-S Batteries.

Accelerating the sluggish sulfur redox kinetics through electrocatalysis has been regarded as one of the key factors to achieve Li-S batteries of cell-level energy densities exceeding 600 Wh kg. Though single-atom catalysts (SACs), typically with symmetric M-N coordination structures have demonstrated attractive electrocatalytic performance in Li-S batteries, herein we discovered that an asymmetric-coordinated metal center distinctly shifts sulfur redox reaction (SRR) kinetics-from first-order (concentration-dependent) behavior in the symmetric-coordinated SACs-to zero-order (surface-saturated) kinetics, highlighting fundamentally altered reaction pathways, leading to a concurrent polysulfide conversion. Experimental and theoretical studies on the Ni atom-based SACs showed that symmetry breaking raises the Ni d-band center, enabling a monodentate-to-bidentate LiS adsorption transition, which strengthens polysulfide adsorption and shifts the rate-limiting step from sluggish solid-solid transformation (LiS → LiS) to a more favorable liquid-solid conversion (LiS → LiS), effectively lowering the overall energy barrier of the SRR process.

SaeGraphDTI: drug-target interaction prediction based on sequence attribute extraction and graph neural network.

Background: Accurately identifying drug-target interactions (DTI) can greatly shorten the drug development cycle and reduce the cost of drug development. In current deep learning-based DTI prediction models, the extraction of drug and target features is a key step to improve model performance. At the same time, drugs and targets form a complex relational network, and leveraging existing network topological relationships can obtain more comprehensive feature representations.

Improvement of d-Allulose Synthesis from Sucrose through Dual PTS-Dependent Transport in Engineered .

d-Allulose, a rare sugar characterized by its high sweetness and low-calorie profile, is gaining attention in the sweetener market. This study introduces an innovative method for converting sucrose into d-allulose through microbial fermentation. An irreversible synthesis pathway was constructed by expressing the , , , and genes in JM109 (DE3), enhancing substrate utilization via dual PTS-dependent transport of sucrose and d-fructose.

An observational study of the efficacy of combined orthodontic-systemic periodontal treatment.

This study comparatively analyzes the periodontal status of patients at 6 months after combined orthodontic-systemic periodontal treatment for improving clinical treatment protocols. Eighty periodontitis patients treated in our hospital from June 2019 to June 2022 were selected as subjects and randomly divided into observation and control groups, with 40 patients in each group. The observation group underwent combined orthodontic-systemic periodontal treatment; the control group underwent systemic periodontal treatment.

Erratum: cDCBLD2 mediates sorafenib resistance in hepatocellular carcinoma by sponging miR-345-5p binding to the TOP2A coding sequence: Erratum.

[This corrects the article DOI: 10.7150/ijbs.86227.

Exogenous Melatonin Increased Soybean Yield Under Saline-Alkali Stress by Enhancing Plant Carbohydrate Metabolism and Transportation.

Saline-alkali (SA) stress is one of the most widespread abiotic stresses affecting plant growth and productivity worldwide. Melatonin is an auxin-like analogue that can improve plant tolerance to multiple abiotic stresses. Herein, two soybean cultivars, Heihe 49 and Henong 95 were pot cultured and treated with 80 mM SA stress solution (mixed with NaCl:NaCO:NaHCO:NaSO = 1:1:9:9) at the V1 stage.

The Interplay of Cross-Organ Immune Regulation in Inflammation and Cancer.

Organs dynamically interact with each other through immunomodulation to create a systemic immune response and influence disease progression. While traditional studies have tended to focus on single-organ immunity, recent studies have placed greater emphasis on reciprocal immune interactions between organs, such as those between the gut, liver, and brain. However, the precise mechanisms underlying these interorgan immune interactions remain unclear.

Nanoliposome-sericin hydrogel microneedle patches effectively alleviate inflammation and scavenge ROS for the treatment of CKD-aP.

Chronic Kidney Disease-associated Pruritus (CKD-aP) is a debilitating condition commonly observed in patients with chronic kidney disease (CKD), characterized by severe skin itching and inflammation. Despite the availability of oral medications and topical treatments, their efficacy is limited, and systemic side effects are a significant concern. This study presents the development of a novel microneedle system (Cap@Lip/Dife-MNs), designed to address CKD-aP through targeted and sustained drug delivery.

The protective effects of retinoic acid-induced protein 14 on ischemia/reperfusion-induced myocardial apoptosis involves over-autophagy repression.

Uncontrolled activation of autophagy following ischemia/reperfusion (I/R) injury leads to cell death. The superfamily of ankyrin repeat proteins (N-Ank protein) was reported to be involved in autophagy regulation and cardiac protection. Bioinformatics analysis was performed (GSE61592 and GSE160516) and ten N-Ank proteins were differentially expressed in I/R models.

LncRNA CA13/EFTUD2/CA13 caused crossed beak by regulating the mandibular calcification in chicken.

Crossed beak is a beak deformity that reduces feeding and drinking in affected chickens, thereby diminishing chicken production performance. Previous studies have shown that long non-coding RNA CA13 (lncRNA CA13), as an upstream regulator of carbonic anhydrase 13 (CA13), exhibited down-regulated expression in the lateral mandibular condyles of the short bone branch in cross-beaked chickens along with CA13. The study investigated the expression characteristics of lncRNA CA13 and its regulatory relationship with CA13 and elongation factor Tu GTP-binding domain-containing protein 2 (EFTUD2), laying the groundwork for the molecular mechanisms of lncRNA CA13 in chicken mandibular mineralization.

Targeting Neutrophil Extracellular Traps: A New Strategy for the Treatment of Acute Ischemic Stroke Based on Thrombolysis Resistance.

Acute ischemic stroke (AIS) is a life-threatening thrombotic disorder, with intravenous thrombolysis (IVT) serving as the first-line treatment during its acute phase. However, thrombolysis resistance diminishes the success rate of early reperfusion. Recent studies have highlighted neutrophil extracellular traps (NETs) as a critical factor contributing to thrombolysis resistance.

Comprehensive Analysis of the Role of Metabolic Features in Osteoporosis: A Multi-Omics Analysis.

Purpose: This study aims to comprehensively explore the metabolic features related to the pathogenesis of osteoporosis (OP) through multi-omics analysis strategy.

Patients And Methods: Gene expression profiles of OP patients (GSE56815) were downloaded from GEO, and metabolism-related genes (MRGs) were extracted. Plasma samples from 45 OP patients and 18 healthy controls (CON) were collected for metabolomics.

A comparison of the survival outcome of paclitaxel liposome-based chemoradiotherapy with or without rhEndostatin for unresectable esophageal squamous cell carcinoma: a retrospective study.

Objectives: This study aimed to compare the survival outcomes of paclitaxel liposome-based chemoradiotherapy, with or without rhEndostatin, in patients with unresectable locally advanced esophageal squamous cell carcinoma (ESCC).

Methods: Patients with ESCC treated with paclitaxel liposome-based definitive chemoradiotherapy (dCRT), with or without rhEndostatin (E), between February 2015 and June 2020 were included. Patients received induction chemotherapy followed by concurrent chemoradiotherapy, with or without rhEndostatin.

Transcriptome and HS-SPME-GC-MS analysis of key genes and flavor components associated with beef marbling.

Wagyu cattle are well-known for their rich marbling. Qinchuan cattle have slower-depositing marbling than Wagyu cattle. However, because of an increase in the consumer demand for high-quality beef and the increasingly stringent standards of beef quality, improving the marbling grade of Qinchuan cattle has become particularly crucial.