Traf2- and Nck-interacting kinase inhibitors: a patent review（2008 - 2024）.

Introduction: Traf2- and Nck-interacting kinase (TNIK) is a crucial player in various intracellular signaling pathways, including Wnt/β-catenin, cytoskeleton organization, and immune activation. It phosphorylates several key target genes and is widely expressed in different organ systems, such as neural, gastrointestinal, lung, liver, and kidney tissues. TNIK has been implicated in multiple different disease areas, including oncology, neurological diseases, and fibrosis.

Evaluation of Bacillus amyloliquefaciens isolate BX13 for the biocontrol of Fusarium incarnatum, a causal agent of fruit rot in postharvest muskmelon.

Background: Fruit rot, caused by Fusarium spp., is one of the most severe postharvest diseases of muskmelon (Cucumis melo L.).

Engineering the Interfacial Charge Transfer Dynamics by Plasmonic S-Scheme Heterojunctions for Machine-Learning-Assisted Dual-Mode Immunoassays.

The development of photoelectrochemical (PEC)-coupled dual-mode biosensors, combined with multivariate regression analysis, is pivotal for advancing point-of-care disease marker diagnostics. Herein, we present a machine learning (ML)-powered dual-channel immunoassay. This platform integrates plasmonic TiO@NH-MIL-125/Au S-scheme photoelectrode with nanoconfined fluorescent CdSe@ZIF-8 probes.

The relationship between death and hospice care attitudes among nursing interns: a cross-sectional survey.

Objective: The aim of this article is to explore the attitudes towards death and hospice care among nursing interns, as well as their relationship, and to analyze the influencing mechanisms.

Background: As an indispensable part of the hospice care multidisciplinary team, nurses play an important role in patient hospice care. As new members of the nursing team, nursing interns will be directly or indirectly involved in the care of dying patients during the clinical practice.

Applications and challenges of DNA barcoding in rapid radiation groups: (Crassulaceae) as a case study.

Objective: et (Hongjingtian in Chinese, RCRR), the roots and rhizomes of and its application in the medicinal market is very chaotic. In this study, DNA barcoding database and identification engine of species were established, decoction pieces from the medicinal market were identified, and the application and challenges of DNA barcoding in the rapid radiation of species were analyzed. This study provides reference for the protection, rational development, and utilization of endangered resources within species.

Transcriptomic and Metabolomic Explanation of the Interaction between Soybean and Root Rot Caused by .

Soybean root rot (SRR), a severe soil-borne disease, hinders continuous soybean cropping, but the interaction mechanism between soybean varieties and (the dominant pathogen) remains unclear. Using buried root inoculation, 64 germplasms were screened: Heinong 531 (HN531) showed strong resistance, while Dongsheng 1 (DS1) was susceptible. Metabolomic and transcriptomic analyses revealed that phenylpropanoid and flavonoid biosynthesis pathways, including lignin and flavonoid production, were activated early during infection.

Skin- and hair- inspired nanofibrious hydrogel composite as multimodal sensor for polysomnographic monitoring in sleep apnea syndrome.

Flexible sensors, particularly biomimetic hair-like sensors, have recently garnered considerable interest for the real-time monitoring of sleep apnea syndrome (SAS) due to their exceptional sensitivity in detecting subtle respiratory airflow. However, most existing designs are limited to structural emulation of hair with single-signal responses, while neglecting the critical role of skin-hair functional integration in biological mechanosensation. Herein, we address this limitation by developing skin-hair mimetic sensor based on alkaline-treated polyacrylonitrile/ionic liquid/electrostatic flocked‑carbon fibers (aPAN/IL/CFs) nanofibrous hydrogel.

Landscape of mobile genetic elements and their functional cargo across the gastrointestinal tract microbiomes in ruminants.

Background: Mobile genetic elements (MGEs) drive horizontal gene transfer and microbial evolution, spreading adaptive genes across microbial communities. While extensively studied in other ecosystems, the role of MGEs in shaping ruminant gastrointestinal microbiomes-especially their impact on diversity, adaptation, and dietary responsiveness-remains largely unexplored. This study systematically profiles MGE distribution and functionality across gastrointestinal regions in multiple ruminant species to advance our understanding of microbial adaptation.

Hydrate-melt electrolyte design for aqueous aluminium-bromine batteries with enhanced energy-power merits.

Aluminium-based aqueous batteries hold promises for next-generation sustainable and large-scale energy storage due to the favorable metrics of Al and water-based electrolytes. However, the performance of current aluminium-based aqueous batteries falls significantly below theoretical expectations, with a critical bottleneck of realizing cathodes with high areal capacities. Herein, we present a hydrate-melt electrolyte design utilizing cost-effective AlCl and organic halide salts, which enables the demonstration of aqueous Al-Br batteries with enhanced energy-power characteristics.

BroadAMP-GPT: AI-Driven generation of broad-spectrum antimicrobial peptides for combating multidrug-resistant ESKAPE pathogens.

Antimicrobial peptides (AMPs) are promising candidates to address the global antimicrobial resistance crisis, yet their traditional design remains labor-intensive and inefficient. Here, we developed BroadAMP-GPT, an integrated computational-experimental framework that combines AI-driven generation, multi-tiered screening, and experimental validation to rapidly discover potent AMPs with broad-spectrum activity. Using this platform, 57% of AI-generated candidates exhibited potent efficacy against ESKAPE pathogens - key culprits of multidrug-resistant infections.

Rational Design and Identification of ISM7594 as a Tissue-Agnostic FGFR2/3 Inhibitor.

The selective inhibition of fibroblast growth factor receptors (FGFR) presents a significant challenge due to the high degree of sequence and the close structural similarity of the subtypes. Herein, we designed selective dual FGFR2/3 inhibitors based on the in-depth understanding of protein-ligand interaction contributions. We efficiently identified ISM7594 () with distinctive flexible hinge binder and a unique central core that potently inhibited FGFR2/3 and selectively spared FGFR1/4.

Removal of CL-20 from waste acid using macroporous resin: Stabilization of waste acid and recovery of CL-20.

Hexanitrohexaazaisowurtzitane (CL-20) is a powerful explosive with significant environmental and ecotoxicological concerns. Industrial production of CL-20 generates acidic effluents containing residual CL-20, posing treatment challenges and resource loss. This study investigated four macroporous resins (DA201-C, NKA-2, D730, and D121) for CL-20 removal and recovery.

Latent class analysis of depression among patients with non-small cell lung cancer after surgery: A cross-sectional study.

Background: Depression exhibits heterogeneity. However, limited research has explored this heterogeneity in patients with non-small cell lung cancer (NSCLC) after surgery from a person-centered perspective. This study aimed to identify classes of depression using latent class analysis (LCA) in patients with NSCLC after surgery and to explore the association between these classes and demographic and clinical characteristics, physical symptoms, distress disclosure, and relationship quality.

Preparation of Highly Antibacterial MXene Nanofiltration Membranes and Investigation of Their Separation Performance.

In this study, polyethersulfone (PES)/sulfonated polyethersulfone (SPES) composite nanofiltration membranes doped with different contents of monolayer titanium carbide nanosheets (TiCT) were prepared by the nonsolvent induced phase inversion (NIPS) method. The effects of TiCT on membrane structure, separation performance and antibacterial activity were investigated systematically. The results demonstrated that the viscosity of the casting solution increased significantly with the increasing content of TiCT.

Advances in organ protection: ischemic preconditioning and erythropoietin as protective strategies.

Background: Ischemic preconditioning (IPC) and erythropoietin (EPO) are known for their protective effects against organ damage caused by ischemia, ischemia-reperfusion (I/R), and certain drugs. This review aims to summarize the literature on IPC and EPO, focusing on their mechanisms and exploring their relationship in organ protection.

Methods: A comprehensive search of PubMed databases was conducted using keywords, such as IPC, EPO, ischemia-reperfusion, and organ protection.

Identification, pathogenic mechanism and control of Stagonosporopsis cucurbitacearum causing post-harvest fruit rot in melon.

Background: Fruit rot, caused by a range of pathogens, represents one of the most devastating post-harvest diseases of melon (Cucumis melo L.). In 2023, we isolated a pathogenic fungus, identified as Stagonosporopsis cucurbitacearum, from infected melon fruits at the a melon cultivation site in Heilongjiang Province, China.

Ammonia-Assisted Chemical Vapor Deposition Growth of Two-Dimensional Conjugated Coordination Polymer Thin Films.

As emerging electroactive materials, the controlled synthesis of highly ordered two-dimensional (2D) conjugated coordination polymer (c-CP) films ensuring the long-range π-electron delocalization is essential for advancing high-performance (opto-)electronics. Here, we demonstrate the growth of highly crystalline 2D c-CP thin films on inert substrates by chemical vapor deposition with the assistance of ammonia (NH) for the first time, leveraging its deprotonation effect on ligands and competing effect as additional coordinating species. The resulting Fe-HHB (HHB = hexahydroxybenzene) films exhibit large-area uniformity and a 2 order-of-magnitude increase in crystal grain size, which translates into significant improvements in electrical conductivity (from 0.

Type One Protein Phosphatase 4aD Negatively Regulates Cotton () Salt Tolerance by Inhibiting the Phosphorylation of Kinases That Respond to Abscisic Acid.

Salinity is one of the major factors limiting the growth, development, and yield of cotton. Although the mechanisms of cotton tolerance to salt stress have been studied, the regulatory roles and mechanisms of protein kinases and phosphatases in cotton salt response remain poorly understood. Here, we identify Type One Protein Phosphatase 4aD (), belonging to the Type One Protein Phosphatase (TOPP) family, as a negative regulator in cotton salt stress response.

Structural Basis of SARS-CoV-2 Nsp13-Derived Peptide-Mediated NK Cell Activation.

As pivotal effectors of antiviral immunity, natural killer (NK) cells are crucial for controlling the spread of COVID-19. The nonstructural protein 13 of SARS-CoV-2 can encode a viral peptide (Nsp13) preventing human leukocyte antigen E (HLA-E) from recognizing inhibitory receptor NKG2A, thereby activating NK cells. The underlying molecular mechanisms of Nsp13 remain unclear.

Construction of safer hirudin-derived peptides with enhanced anticoagulant properties based on C-terminal active residue adaptation and modification.

Introduction: Hirudin exerts anticoagulant effects by inhibiting the binding and catalytic activity of thrombin to fibrinogen. However, its rigid N-terminal region irreversibly occupies the thrombin catalytic center, raising concerns about potential bleeding.

Objectives: In this study, a novel lead compound, WPHVC_V1, which is based on the competitive binding mechanism of the hirudin variant WPHV_C, was developed and validated for in vitro and in vivo activity and safety.

The Effect of "Online-Simulation-Bedside" Three-Step Teaching Method in Team Cardiopulmonary Resuscitation Skills Training of Emergency Department and Critical Care Nursing Interns-An Analysis Based on Kirkpatrick Model.

This study designed a three-step teaching method of "online-simulation-bedside," which was applied to the teaching of cardiopulmonary resuscitation skills in the team of emergency and critical care nursing interns, and the effectiveness of this teaching method was evaluated using the Kirkpatrick model. Mastering knowledge of cardiopulmonary resuscitation and emergency techniques is necessary for nursing interns to fulfill their roles. At present, the first aid awareness and rescue skills of nursing interns are not optimistic.

Study on the adsorption mechanism of CL-20 in waste acid by silicic acid: adsorption model and DFT calculation.

Industrial wastewater pollution has emerged as a significant global environmental challenge, particularly regarding the urgent need to address highly acidic industrial effluents containing organic pollutants. As a novel high-energy explosive, hexanitrohexaazaisowurtzitane (CL-20) inevitably generates strongly acidic wastewater during its production and application processes, posing severe threats to ecological systems and human health. This study systematically investigates the adsorption performance of two silicon-based materials, sodium silicate (NaSiO) and silicic acid (HSiO), for the removal of CL-20 from wastewater.

Long-offset paired nicking-based efficient and precise strategy for in vivo targeted insertion.

Clustered regularly interspaced short palindromic repeat (CRISPR)-based targeted insertion of DNA fragments holds great promise for gene therapy. However, designing highly efficient and precise integration of large DNA segments in somatic cells while avoiding unpredictable products remains challenging. Here, we devised a novel long-offset paired nicking target integration (LOTI) strategy, which enhances the capacity of Cas9 nickase (Cas9n) in targeted gene integration in somatic cells, yielding higher knock-in (KI) efficiency compared with classical nickase-based approaches.

The crosstalk of monocyte-neutrophil in hair follicles regulates neutrophil transepidermal migration in contact dermatitis.

The excessive accumulation of neutrophils within the epidermis is a significant hallmark of cutaneous diseases; however, the mechanisms governing neutrophil transepidermal migration (NTEM) remain inadequately understood. In this study, we develop trichromatic-fluorescence-labeled chimeric mice by utilizing Cx3cr1Lyz2 mice as bone marrow donors and Krt14 mice as recipients. This approach enables us to visualize the process of NTEM and the crosstalk between neutrophils and monocytes in a murine model of irritant contact dermatitis (ICD).

Satellite Remote Sensing-Implemented Nontargeted Screening of Emerging Contaminant Fingerprints in a River-to-Ocean Continuum through Interpretable Machine Learning: The Pivotal Intermediary Role of Dissolved Organic Matter.

Emerging contaminants (ECs) can exert irreversible health impacts on humans, even at trace concentrations. Currently, nontargeted screening of ECs has been developed for their assessment, which requires sophisticated instrumentation. Although satellite remote sensing is a cost-effective technology for water quality assessment, accurately measuring ECs in a river-to-ocean continuum remains a significant challenge due to their trace levels.