Identification of an antifungal lipopeptide from HAU3 inhibiting the growth of using preparative chromatography and 2D-NMR.

Unlabelled: The presence of fungal contamination and its mycotoxins in animal feed is pervasive, posing a significant threat to the well-being and performance of animals, as well as the safety of animal-derived food products. In this work, we screened a strain of () HAU3 that exhibits efficient antifungal activity against the growth of (). The antifungal activity was detected in the supernatant, with 20% sterile supernatant demonstrating an impressive antifungal rate of 98.

Effect of berberine on LPS-induced intestinal epithelial injury and mA methylation in broilers.

This study sought to explore the effects of and potential mechanisms underlying berberine (BBR) in lipopolysaccharide (LPS)-induced intestinal epithelium injury in broilers. Ninety 19-day-old male AA broilers were randomly divided into five groups: the negative control group (NC), positive control group (PC), and three treatment groups (T1, T2, and T3). On the 21st day of age, the control group was orally administered saline, the LPS-challenged group was orally administered LPS (3 mg/kg body weight), and the treatment groups were orally administered BBR (6, 12, 18 mg/kg body weight) along with LPS (3 mg/kg body weight).

Exploring the gut microbiota associated with peripheral nerve invasion in colorectal cancer patients and constructing predictive models.

Objective: This study aims to explore the differences in composition, abundance, and biological functions of the gut microbiota between colorectal cancer (CRC) patients with peripheral nerve invasion (PNI) and those without peripheral nerve invasion (NPNI). Additionally, we tried to construct a machine-learning predictive model incorporating the identified microbiota characteristics to explore the impact of gut microbiota on CRC-PNI progression and to search for new non-invasive microbiological indicators for CRC-PNI. Finally, we successfully developed a predictive model to predict PNI in CRC patients through leveraging microbial biomarkers.

LincRNA-MSTRG.673.2 Promotes Chicken Intramuscular Adipocyte Differentiation by Sponging miR-128-3p.

Intramuscular fat content is positively correlated with meat flavor and juiciness. Increasing the intramuscular fat (IMF) content of chickens while increasing their growth rate has become a hot topic in molecular breeding. The group's previous studies showed that miR-128-3p inhibited chicken intramuscular adipocyte differentiation and lipogenesis.

Association of sleep patterns with microvascular complications in individuals with type 2 diabetes: A prospective cohort study.

Background: This study aimed to explore the association between sleep patterns and the risk of microvascular complications in patients with type 2 diabetes.

Methods And Results: Using UK Biobank data, we included the findings for 19,996 patients with type 2 diabetes who were free of diabetic microvascular complications at baseline. The sleep-pattern score was calculated as the sum of the scores for six low-risk sleep-pattern behaviors encompassing sleep duration, chronotype, daytime sleepiness, snoring, insomnia, and daytime napping.

Epigenomic insights into the immune regulatory mechanism of GBP4L in poultry.

High-temperature environments induce heat stress in poultry, leading to compromised immune function and reduced disease resistance. In the Dexamethasone-induced stress-related immune suppression model in chickens, both transcriptomic and proteomic analyses revealed significantly low expression of GBP4L, highlighting its potential as a key target for combating stress. It is currently unclear whether GBP4L can alleviate the heat stress response in the body.

Intestinal differential metabolite tryptophan from avian pathogenic Escherichia coli (APEC)-resistant and susceptible chickens alleviates APEC symptoms in chickens.

To identify an effective antibiotic to combat avian pathogenic Escherichia coli (APEC), differential metabolites in the intestines of APEC-resistant and susceptible chickens were investigates. The effect of supplementing the diet of APEC-challenged chickens with the differential metabolite tryptophan was investigated. Tryptophan was identified as an important differential gut metabolite (APEC-resistant and susceptible chickens) using tryptophan-targeted metabolism detection.

Integrated analysis of tyrosine-induced MiRNA and mRNA expression profiles in melanocytes reveals the regulatory role of miR-1560-3p in melanin deposition in Xichuan black-bone chickens.

Background: Tyrosine is a prerequisite for melanin biosynthesis and plays a crucial role in the growth and development of melanocytes, but the underlying mechanism is still unclear. In our previous research, we added 10-10 mol/L tyrosine to the melanocytes of black-bone chickens and found that 10 mol/L tyrosine significantly increased the tyrosinase content in melanocytes.

Methods: In this study, melanocytes from Xichuan black-bone chickens were used as research objects, 10 mol/L tyrosine was added for transcriptome sequencing.

The m6A RNA demethylase FTO promotes radioresistance and stemness maintenance of glioma stem cells.

Glioblastoma (GBM) was the most common and deadliest malignant brain tumor in adults, with a poor prognosis. Effective targeted drugs are still lacking, and the presence of glioblastoma stem cells (GSC) is a major factor contributing to radiotherapy resistance. Screening for targeted drugs that can sensitize GBM to radiotherapy is crucial.

miR-200b-3p affects the proliferation and differentiation of chicken preadipocytes by modulating SESN1 expression through competition with CircADGRF5.

Excessive deposition of abdominal fat in chickens has a significant impact on the poultry industry, and there is increasing evidence that non-coding RNAs play a crucial role in fat development. In our previous RNA-seq study, miR-200b-3p was found to be differentially expressed during different developmental periods of fat in Gushi chickens. In this study, we report that miR-200b-3p can directly bind to the 3'UTR region of SESN1 to promote proliferation and inhibit differentiation of preadipocytes.

Multi-Omics Profiling of Lipid Variation and Regulatory Mechanisms in Poultry Breast Muscles.

This study aimed to elucidate the genetic basis of lipid composition in the breast muscles of poultry, including AA broilers, dwarf guinea fowl, quails, and pigeons, and the impact of artificial selection on lipid traits. By employing lipidomics and transcriptomic sequencing, the research analyzed the chest muscle tissues of these four poultry. A total of 1542 lipid molecules were identified, with 711 showing significant differences among species.

Multi-Omics Insights into Regulatory Mechanisms Underlying Differential Deposition of Intramuscular and Abdominal Fat in Chickens.

Excessive abdominal fat deposition in chickens disadvantages feed conversion, meat production, and reproductive performance. Intramuscular fat contributes to meat texture, tenderness, and flavor, serving as a vital indicator of overall meat quality. Therefore, a comprehensive analysis of the regulatory mechanisms governing differential deposition of abdominal versus intramuscular fat is essential in breeding higher-quality chickens with ideal fat distribution.

gga-miR-6634-5p Affects the proliferation and steroid hormone secretion of chicken (Gallus Gallus) granulosa cells by targeting MMP16.

MiRNAs are typically reported to play a negative regulatory role in post-transcriptional expression of target genes and are widely involved in a variety of biological processes such as growth, metabolism and reproduction. However, research on the role of miRNAs in the ovulation process of chicken ovaries is still insufficient compared to that in mammals. Here, we investigated the regulatory mechanisms of gga-miR-6634-5p in the growth and steroid hormone secretion of chicken granulosa cells (GCs) by targeting MMP16.

CircITGB5 regulates the proliferation and adipogenic differentiation of chicken intramuscular preadipocytes through the miR-181b-5p/CPT1A axis.

Non-coding RNA is known to play a crucial role in the generation and deposition of intramuscular fat (IMF) in vertebrates by regulating the expression of genes involved in the synthesis, degradation and transportation of IMF. With the introduction of the competing endogenous RNA (ceRNA) hypothesis, circular RNA (circRNA) and long non-coding RNA (lncRNA) have been identified as natural "sponges" for microRNA (miRNA), yet their precise mechanisms and biological functions in chicken IMF are still not fully understood. In earlier research, we observed a significant association between Carnitine O-palmitoyltransferase 1, liver isoform (CPT1A) and fatty acid metabolism in chicken breast muscle.

Localization and expression of C1QTNF6 in chicken follicles and its regulatory effect on follicular granulosa cells.

C1q/tumor necrosis factor-related protein 6 (C1QTNF6) is a newly discovered adiponectin analog that plays a significant role in female reproduction. However, its expression and function in chickens remain unclear. In this study, the full-length coding sequence (CDS) of chicken C1QTNF6 was cloned from adult chicken ovary.

Deletion of the gsk-3β (Glycogen synthase kinase-3β) in zebrafish results in decreased susceptibility to Aeromonas hydrophila.

Aeromonas hydrophila is a significant pathogen in the field of fish farming, resulting in substantial financial losses for the aquaculture industry. As the pathogen's resistance to commercially available antibiotics continues to rise, the identification of novel antimicrobial strategies becomes increasingly crucial. This study aims to explore the modulatory impact of gsk-3β (Glycogen synthase kinase-3β) on the intrinsic immunity against Aeromonas hydrophila in zebrafish, with the objective of uncovering a new avenue for enhancing fish antimicrobial activity through gene editing.

LncRNA Facilitates Lipid Deposition by Promoting the Ubiquitination of MYH9 in Chicken LMH Cells.

The liver plays an important role in regulating lipid metabolism in animals. This study investigated the function and mechanism of in liver lipid metabolism in hens at the peak of egg production. The effect of on intracellular lipid content in LMH cells was evaluated by qPCR, Oil Red O staining, and detection of triglyceride (TG) and cholesterol (TC) content.

Plasma exosome-derived miR-455-5p targets RPS6KB1 to regulate cartilage homeostasis in valgus-varus deformity (Gallus gallus).

Valgus-varus deformity (VVD) is a common long bone deformity in broilers. Imbalance in cartilage homeostasis is the main feature of leg disease. Exosomes act as an important intercellular communication vector that regulates chondrogenesis by encapsulating specific nucleic acids and proteins.

Genome-wide variation study and inter-tissue communication analysis unveil regulatory mechanisms of egg-laying performance in chickens.

Egg-laying performance is of great economic importance in poultry, but the underlying genetic mechanisms are still elusive. In this work, we conduct a multi-omics and multi-tissue integrative study in hens with distinct egg production, to detect the hub candidate genes and construct hub molecular networks contributing to egg-laying phenotypic differences. We identifiy three hub candidate genes as egg-laying facilitators: TFPI2, which promotes the GnRH secretion in hypothalamic neuron cells; CAMK2D, which promotes the FSHβ and LHβ secretion in pituitary cells; and OSTN, which promotes granulosa cell proliferation and the synthesis of sex steroid hormones.

The role of AdipoQ on proliferation, apoptosis, and hormone Secretion in chicken primary adenohypophysis cells.

Adiponectin (AdipoQ), an adipokine secreted by adipocytes, has been reported to exist widely in various cell types and tissues, including the adenohypophysis of chickens. However, the molecular mechanism by which AdipoQ regulates the function of chicken adenohypophysis remains elusive. In this study, we investigated the effects of AdipoQ on proliferation, apoptosis, secretion of related hormones (FSH, LH, TSH, GH, PRL and ACTH) and expression of related genes (FSHβ, LHβ, GnRHR, TSHβ, GH, PRL and ACTH) in primary adenohypophysis cells of chickens by using real-time fluorescent quantitative PCR (RT-qPCR), cell counting kit-8 (CCK-8), flow cytometry, enzyme-linked immunosorbent assay (ELISA) and Western blot (WB) assays.

Estrogen promotes gonadotropin-releasing hormone expression by regulating tachykinin 3 and prodynorphin systems in chicken.

The "KNDy neurons" located in the hypothalamic arcuate nucleus (ARC) of mammals are known to co-express kisspeptin, neurokinin B (NKB), and dynorphin (DYN), and have been identified as key mediators of the feedback regulation of steroid hormones on gonadotropin-releasing hormone (GnRH). However, in birds, the genes encoding kisspeptin and its receptor GPR54 are genomic lost, leaving unclear mechanisms for feedback regulation of GnRH by steroid hormones. Here, the genes tachykinin 3 (TAC3) and prodynorphin (PDYN) encoding chicken NKB and DYN neuropeptides were successfully cloned.

LncRNA lncMGR regulates skeletal muscle development and regeneration by recruiting CDK9 and sponging miRNAs.

Long non-coding RNAs (lncRNAs) play an essential role in vertebrate myogenesis and muscle diseases. However, the dynamic expression patterns, biological functions, and mechanisms of lncRNAs in skeletal muscle development and regeneration remain largely unknown. In this study, a novel lncRNA (named lncMGR) was differentially expressed during breast muscle development in fast- and slow-growing chickens.

Genomic Insights into Molecular Regulation Mechanisms of Intramuscular Fat Deposition in Chicken.

Intramuscular fat (IMF) plays an important role in the tenderness, water-holding capacity, and flavor of chicken meat, which directly affect meat quality. In recent years, regulatory mechanisms underlying IMF deposition and the development of effective molecular markers have been hot topics in poultry genetic breeding. Therefore, this review focuses on the current understanding of regulatory mechanisms underlying IMF deposition in chickens, which were identified by multiple genomic approaches, including genome-wide association studies, whole transcriptome sequencing, proteome sequencing, single-cell RNA sequencing (scRNA-seq), high-throughput chromosome conformation capture (HiC), DNA methylation sequencing, and mA methylation sequencing.

Characteristics of conserved microRNAome and their evolutionary adaptation to regulation of immune defense functions in the spleen of silver carp and bighead carp.

Immune defense functions of silver carp (Hypophthalmichthys molitrix) and bighead carp (Hypophthalmichthys nobilis) have shown obvious evolutionary divergence. MiRNAs participate in the fine regulation of immune function. However, the evolutionary adaptation of miRNAs in the regulation of immune defense function is still poorly understood in silver carp and bighead carp.