Effect of defective H2B ubiquitination on the malignancy and repair of DNA double breaks and its significance in lung adenocarcinoma.

Lung cancer is the leading cause of cancer-related mortality, with a notably low five-year survival rate due to the limited availability of effective treatments. Monoubiquitination of histone H2B at lysine 120(uH2B) exerts a tumor-suppressive effect in cancer through multiple mechanisms, including the regulation of transcriptional activity and DNA damage response. Ring Finger Protein 20 (RNF20), a key E3 ubiquitin ligase, plays a central role in this process.

Evaluating land capability using site assessment and land evaluation model in GIS in a watershed scale.

Assessment of the capability of agricultural lands using a precise and systematic approach is vital to identify both the primary limitations and the potential of the land. With the growing need to protect farmlands, particularly in areas at high risk of degradation, developing an effective evaluation method is essential. This study focuses on site-specific conditions in China, where such risks are prevalent, and integrates the Land Evaluation and Site Assessment (LESA) method with ArcGIS to map and evaluate agricultural land potential.

LncRNA-Gm17586 inhibits S.typhimurium mediated pyroptosis by promoting NLRP3 and Tnip1 binding.

The aim of this study is to identify the lncRNA that directly interacts with NLRP3 molecules in RAW264.7 cells infected with S. typhimurium and its role in S.

Knocking Down LncRNA-GAS5 Restores the Inhibition of Cuprotosis Protein FDX-1 by .

This study investigated the regulatory effect of LncRNA-GAS5 on FDX-1 in HEK293T cells by . Transfected LncRNA-GAS5 overexpressing or knocking down plasmids into HEK293T cells, and the expression of FDX-1 was detected by Western blotting. inhibited the expression of FDX-1.

Revealing Ultrafast Optical Nonlinearity of Trapped Exciton Polaritons in Atomically Thin Semiconductors.

Nonlinearities are fundamental to modern optical technologies. Exciton polaritons in semiconductor microcavities provide a promising route to strong nonlinearities. Monolayer TMDs, with tightly bound excitons and strong oscillator strength, enable polaritonic phenomena under ambient conditions but face challenges from weak polariton interactions due to small exciton Bohr radius.

Plasma exosome-derived miR-455-5p targets RPS6KB1 to regulate cartilage homeostasis in valgus-varus deformity (Gallus gallus).

Valgus-varus deformity (VVD) is a common long bone deformity in broilers. Imbalance in cartilage homeostasis is the main feature of leg disease. Exosomes act as an important intercellular communication vector that regulates chondrogenesis by encapsulating specific nucleic acids and proteins.

Syntaxin17 Restores Lysosomal Function and Inhibits Pyroptosis Caused by Acinetobacter baumannii.

Acinetobacter baumannii (A. baumannii) causes autophagy flux disorder by degrading STX17, resulting in a serious inflammatory response. It remains unclear whether STX17 can alter the inflammatory response process by controlling autolysosome function.

3-Amide-β-carbolines block the cell cycle by targeting CDK2 and DNA in tumor cells potentially as anti-mitotic agents.

β-Carboline alkaloids are natural and synthetic products with outstanding antitumor activity. C substituted and dimerized β-carbolines exert excellent antitumor activity. In the present research, 37 β-carboline derivatives were synthesized and characterized.

Path analysis of regional logistics and economy coordinated development: An fsQCA approach.

The coordinated development of regional logistics and the economy is crucial for regional economic progress and for reducing regional development disparities. This study applies regional coordinated development theory and coupling theory, utilizing the Coupling Coordination Degree Model (CCDM) to analyze data from 31 provinces and cities in China in 2021, with the analysis results serving as the outcome variable. Additionally, we use data from four dimensions: infrastructure investment (II), technological innovation (TI), industrial structure (IS), and human capital (HC), as the conditional variables, conducting a multi-factor configurational analysis using fsQCA.

A Dual-Threshold Algorithm for Ice-Covered Lake Water Level Retrieval Using Sentinel-3 SAR Altimetry Waveforms.

Satellite altimetry has been proven to measure water levels in lakes and rivers effectively. The Sentinel-3A satellite is equipped with a dual-frequency synthetic aperture radar altimeter (SRAL), which allows for inland water levels to be measured with higher precision and improved spatial resolution. However, in regions at middle and high latitudes, where many lakes are covered by ice during the winter, the non-uniformity of the altimeter footprint can substantially impact the accuracy of water level estimates, resulting in abnormal readings when applying standard SRAL synthetic aperture radar (SAR) waveform retracking algorithms (retrackers).

Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying.

Induced molting is a common method to obtain a new life in laying hens, in which periodic changes in feathers are the prominent feature. Nevertheless, its precise molecular mechanism remains unclear. In this study, feather and hair follicle samples were collected during fasting-induced physiological remodeling for hematoxylin-eosin staining, hormone changes and follicle traits, and transcriptome sequencing.

Evaluating the Performance of Seven Ongoing Satellite Altimetry Missions for Measuring Inland Water Levels of the Great Lakes.

Satellite altimetry can provide long-term water level time series for water bodies lacking hydrological stations. Few studies have evaluated the performance of HY-2C and Sentinel-6 satellites in inland water bodies, as they have operated for less than 1 and 2 years, respectively. This study evaluated the measured water level accuracy of CryoSat-2, HY-2B, HY-2C, ICESat-2, Jason-3, Sentinel-3A, and Sentinel-6 in the Great Lakes by in-situ data of 12 hydrological stations from 1 January 2021 to 1 April 2022.

1-Formyl--carboline Derivatives Block Newcastle Disease Virus Proliferation through Suppressing Viral Adsorption and Entry Processes.

Newcastle disease virus (NDV) is one of the highly contagious pathogens causing devastating economic effects on the global poultry industry. In the present study, three 1-formyl--carboline derivatives (compounds , , and ) were found to be potent inhibitors of different genotypes of NDV with IC values within 10 μM, which are similar to ribavirin. The virus titers were decreased by the presence of 1-formyl--carboline derivatives in a dose-dependent manner, and the inhibition rate was found to exceed 90% at the concentration of 20 μM.

up-regulates LncRNA-GAS5 and promotes the degradation of STX17 by blocking the activation of YY1.

autophagy, affects the degradation of autophagy, and causes severe inflammatory injury. LncRNA growth arrest-specific transcript 5 (LncRNA-GAS5) and Yin and Yang 1 (YY1) are known to play an important role in the regulation of autophagy, however, the precise role of LncRNA-GAS5 and YY1 in the damage to autophagy caused by unclear. The aim of this study was to investigate the role of LncRNA-GAS5 and YY1 in the regulation of autophagy induced by .

Acinetobacter baumannii outer membrane protein A induces HeLa cell autophagy via MAPK/JNK signaling pathway.

Autophagy is an evolutionary conserved self-balancing process that plays an important role in maintaining cellular homeostasis via the clearance of damaged organelles and misfolded proteins. Infection-triggered autophagy specifically inhibits the invasion of intracellular bacterial replication and hence protects the cells from microbial infections. It has been reported that Acinetobacter baumannii trigger cell autophagy.

Acinetobacter baumannii outer membrane protein 34 elicits NLRP3 inflammasome activation via mitochondria-derived reactive oxygen species in RAW264.7 macrophages.

Acinetobacter baumannii (A. baumannii) is a Gram-negative bacterium, which acts as an opportunistic pathogen and causes hospital-acquired pneumonia and bacteremia by infecting the alveoli of epithelial cells and macrophages. Evidence reveals that A.

Surgical treatment of syphilitic superficial femoral artery aneurysm: a rare case report.

Background: Arterial aneurysm is a known complication of syphilis, but the occurrence of femoral artery aneurysm secondary to the syphilitic disease has never been reported.

Case Presentation: The present study described a 60-year-old Chinese male who presented with two aneurysms in the middle and lower segment of the right superficial femoral artery causing the symptoms of pain, coldness and numbness in the right lower limb. This case was diagnosed with syphilitic superficial femoral aneurysm because of positive syphilitic testing and the inflammatory cell infiltration around the adventitial vasa vasorum under the pathological examination.

[Species identification and absolute quantification of biological samples by droplet digital PCR].

Objective: To test droplet digital PCR for species identification and absolute quantification of biological sample.

Methods: Specific primers and probes for human mtDNA encoding gene ND4 and 16S rRNA were designed, and the species-specificity was assessed on DNA samples derived from human and common animals. To determine the sensitivity and stability of droplet digital PCR for species identification and absolute quantification, gradient dilution series of recombinant plasmid and 16 human DNA samples were analyzed.

Coagulation and fibrinolytic indices during the first trimester of pregnancy in women with polycystic ovary syndrome: a preliminary study.

Objective: To evaluate the levels of coagulation and fibrinolytic markers during the first trimester of pregnancy in women with polycystic ovary syndrome (PCOS) and determine the effects of PCOS and obesity on the levels of these hemostatic markers.

Methods: A cross-sectional study was conducted in Beijing, China, on women with PCOS (n = 50), healthy women (n = 50), pregnant women with PCOS (n = 50), and healthy pregnant women (n = 50) at 12 weeks of pregnancy. Coagulation and fibrinolytic parameters were measured.

[Abnormalities of microsatellite in transitional cell carcinoma of urinary bladder related with aromatic amine exposure].

Objective: To study the microsatellite abnormalities of the aromatic amine exposure-associated transitional cell carcinoma (TCC) and sporadic TCC of urinary bladder, and to evaluate the potential of microsatellite analysis on detection of this diseases.

Methods: Based on our previous investigations, 5 microsatellite markers (D17S695, D9S162, D3S1295, DBH and D3S1234) that had high frequencies of loss of heterozygosity (LOH) in sporadic TCC, were selected for analysis with the bladder lesions derived from 16 patients with aromatic amine exposure history. The microsatellite analysis with urine sediments from the post-operated patients was also carried out.