Exploring the gut microbiota associated with peripheral nerve invasion in colorectal cancer patients and constructing predictive models.

Objective: This study aims to explore the differences in composition, abundance, and biological functions of the gut microbiota between colorectal cancer (CRC) patients with peripheral nerve invasion (PNI) and those without peripheral nerve invasion (NPNI). Additionally, we tried to construct a machine-learning predictive model incorporating the identified microbiota characteristics to explore the impact of gut microbiota on CRC-PNI progression and to search for new non-invasive microbiological indicators for CRC-PNI. Finally, we successfully developed a predictive model to predict PNI in CRC patients through leveraging microbial biomarkers.

Tailoring Plasmid Design Based on Chain Expression in Cell Line Development for Enhanced Monoclonal and Bispecific Antibody Production.

The development of robust, high-yielding cell lines represents a critical factor in establishing efficient and cost-effective manufacturing processes within the competitive biopharmaceutical industry. While extensive research has focused on optimizing plasmid design, host cell characteristics, and selection strategies to enhance cell line development, this study specifically investigates strategic expression plasmid design to improve both productivity and product quality of therapeutic proteins in Chinese Hamster Ovary (CHO) cells using transposon technology. Our findings demonstrate that increasing light chain and heavy chain copy numbers while maintaining balanced expression significantly enhances productivity and mitigates purity risks.

Identification and predictive machine learning models construction of gut microbiota associated with lymph node metastasis in colorectal cancer.

This study focuses on the significant role between gut microbiota and lymph node metastasis (LNM) in colorectal cancer (CRC). By conducting 16S rRNA sequencing on fecal samples from 147 CRC patients and combining it with the linear discriminant analysis effect size algorithm, we successfully identified significant differences in the gut microbiota between patients with LNM and those with no lymph node metastasis (NLNM). Furthermore, using transcriptome data from 23 CRC patients, we constructed an immune cell infiltration matrix to deeply explore the biological functions associated with LNM.

Intervention of polyphenols on cod protein modification induced by α, β-unsaturated aldehydes in multi-interface system.

α, β-unsaturated aldehydes, generated by lipid peroxidation (LPO), are significant electrophiles with strong protein modification capacity in various seafood products. Polyphenols have been widely used as potent scavengers of α, β-unsaturated aldehydes to inhibit LPO-related protein deterioration. Compared with the homogenous aqueous system focused by most studies, heterogeneous multi-interface systems occurred more commonly in real food matrix.

The impact of fish oil oxidation on interfacial properties and system stability of cod protein.

In aquatic food multiphase interface systems, proteins and lipids coexist concurrently. Among them, lipids, particularly fish oil rich in polyunsaturated fatty acids, are susceptible to oxidation, resulting in oxidized products exhibiting similar interfacial activity as amphiphilic proteins. However, the interaction between oxidized fish oil products and proteins within the interface system remains unclear.

High-pressure homogenization transformed salmon protein filament into micelle structure: Improvement on the stability and swallowing rheology of dysphagia-oriented salmon emulsion gels.

The aging population is stimulating increased demand for dysphagia-oriented foods, yet most current options are made of ultra-processed macronutrients and lack high-quality protein and ω-3 fatty acids. This study explores the use of whole salmon fillets as a myofibrillar protein source to stabilize salmon backbone oil, creating ω-3-rich emulsion gels (50-60 vol%) for dysphasia individuals. Two-step high-pressure homogenization (HPH; 50 MPa) improved emulsion texture, storage stability, and swallowability (IDDSI level 4) by reducing oil droplet size (from 20 to 2 μm) and increasing elastic modulus by 6-8 times and viscosity by more than 10 times.

A novel micro-aqueous cold extraction of salmon head oil to reduce lipid oxidation and fishy odor: Comparison with common methods.

Traditional heat extraction (HE) has a low efficiency (75.2 wt%) and induces lipid oxidation of PUFAs. The novel micro-aqueous cold (<25 °C) extraction (MAE) was applied to extract salmon head oil.

A case of primary hepatic stromal tumour misdiagnosed as a liver cyst.

Primary hepatic stromal tumours are very rare and there are only sporadic reports in the literature. Due to the lack of specificity in their clinical manifestations and imaging features, these tumours are easily misdiagnosed. This current report presents a case of primary liver stromal tumour that was misdiagnosed as a liver cyst.