Epidermal Growth Factor Receptor (EGFR)-Targeting Peptides and Their Applications in Tumor Imaging Probe Construction: Current Advances and Future Perspectives.

The epidermal growth factor receptor (EGFR) is a key target for both cancer diagnosis and therapeutic interventions. Assessing EGFR expression before therapy has become routine in clinical practice, yet current methods like biopsy and immunohistochemistry (IHC) have significant limitations, including invasiveness, limited repeatability, and lack of real-time, whole-body data. EGFR-targeted imaging has emerged as a promising alternative.

Design, synthesis, and biological evaluation of Osimertinib-Cy7 (OSA-Cy7) conjugate as potential theranostic agent targeting activating EGFR mutations.

Accurately predicting the therapeutic response of non-small cell lung cancer (NSCLC) patients to tyrosine kinase inhibitors (TKIs) is of significant clinical importance. The use of TKIs in clinical is primarily guided by the detection of EGFR gene mutations. However, the current EGFR mutation assays face challenges such as inconsistent correlation with therapeutic outcomes, inconvenient sample availability and limited sensitivity.

Strong Nucleating Effect of Si-Containing Tri-Block Oligomers on Poly(Ethylene Terephthalate).

The development of a silane coupling agent with an aminopropyl structure as a nucleating agent for poly(ethylene terephthalate) (PET) is reported in this study. The tri-block oligomers nucleating agent was formed by 1,3-bis(3-aminopropyl)-1,1,3,3-tetramethyldisiloxane/oxalic acid/low molecular weight PET (LPOBD). It was subsequently cross-linked with tetraethyl orthosilicate to form LPOBD-T.

The Rab5 effector Rabankyrin-5 mediates endosomal fusion and trafficking of Human Papillomavirus during early entry.

The fusion of newly formed early endosomal vesicles after endocytosis is a crucial step in viral infection. It facilitates the transition of many viruses from viral internalization to downstream intracellular trafficking within the endosomal network, ultimately enabling their delivery to intracellular replication sites. Despite its significance, the molecular mechanisms regulating the fusion of these vesicles remain poorly understood.

Research on the impact of hospital organizational behavior on physicians'patient-centered care.

Objectives: This study seeks to examine the impact of hospital organizational behavior on Physicians' Patient-centered care and aims to offer innovative insights and strategies for enhancing the quality of healthcare services.

Methods: A conceptual model was developed based on organizational behavior theory. Data were collected via questionnaires from 10 large public hospitals in China, encompassing eight independent variables, including hospital organizational culture, change behavior, and motivational behavior, as well as moderating variables like physician title and control variables such as personality traits.

A sleep staging model based on adversarial domain generalized residual attention network.

To solve the problem of poor generalization ability of the model on unknown data and the difference of physiological signals between different subjects. A sleep staging model based on Adversarial Domain Generalized Residual Attention Network (ADG-RANet) is designed. The model is divided into three parts: feature extractor, domain discriminator and label classifier.

"Turn-on" aptamer-immune lateral flow assays for the detection of small molecule targets based on CHA-assisted and CRISPR/Cas12a mediated signal transduction and amplification.

Lateral flow assays (LFAs) have emerged as crucial tools for on-site food safety detection due to their simple operation and intuitive detection results. Nevertheless, LFAs for small molecule targets such as pesticides often present a "Turn-off" signal output, which leads to their low sensitivity and the risk of false positives. In this study, a CRISPR/Cas12a system-mediated strategy was employed to convert aptamer signals into the signals of immune LFAs, achieving a "Turn-on" signal output for highly sensitive detection of small molecule targets.

Assembling ″Balloon-String″ Colorimetric Probes through Click Chemistry and Hybridization Chain Reaction for Dual-Signal Amplification in Aptamer-Based Lateral Flow Assays.

As a simple and visually assessable point-of-care testing method, lateral flow assays (LFAs) are widely used for detecting procymidone in vegetable samples. Meanwhile, an increasing number of aptamer-based LFAs are being developed for rapid detection. However, the sensitivity of conventional gold nanoparticles (AuNPs) aptamer-based LFAs is limited, which is a challenge to meet the detection requirements.

Asialoglycoprotein receptor-targeted perfluorooctylbromide as a targeted contrast agent for evaluating the severity of carbon tetrachloride-induced acute liver damage in rats.

Asialoglycoprotein receptor (ASGPR) is an endocytic C-type lectin receptor in hepatocytes. Acute and chronic liver diseases can result in the decreased expression and content of this receptor. The objective of this study was to determine whether ASGPR-targeted perfluorooctylbromide (PFOB) can enhance ultrasound imaging signals and evaluate the severity of carbon tetrachloride (CCl4)-induced acute liver damage in rats.

Integrated analysis of tyrosine-induced MiRNA and mRNA expression profiles in melanocytes reveals the regulatory role of miR-1560-3p in melanin deposition in Xichuan black-bone chickens.

Background: Tyrosine is a prerequisite for melanin biosynthesis and plays a crucial role in the growth and development of melanocytes, but the underlying mechanism is still unclear. In our previous research, we added 10-10 mol/L tyrosine to the melanocytes of black-bone chickens and found that 10 mol/L tyrosine significantly increased the tyrosinase content in melanocytes.

Methods: In this study, melanocytes from Xichuan black-bone chickens were used as research objects, 10 mol/L tyrosine was added for transcriptome sequencing.

Construction of a user-friendly software-defined networking management using a graph-based abstraction layer.

The software-defined networking (SDN) paradigm relies on the decoupling of the control plane and data plane. Northbound interfaces enable the implementation of network services through logical centralised control. Suitable northbound interfaces and application-oriented abstractions are the core of the SDN ecosystem.

Hepatic arterial infusion chemotherapy combined with lenvatinib and PD-1 inhibitors versus lenvatinib and PD-1 inhibitors for unresectable HCC: a meta-analysis.

Objectives: This study aimed to evaluate the effectiveness of combining hepatic arterial infusion chemotherapy (HAIC) with lenvatinib and programmed cell death protein 1 (PD-1) inhibitors in the treatment of advanced, unresectable hepatocellular carcinoma (HCC).

Methods: A comprehensive search across multiple databases was conducted to identify relevant studies published up to May 2024. This search focused on clinical trials investigating the combination of HAIC with lenvatinib and PD-1 inhibitors for the treatment of advanced HCC.

Boanmycin overcomes bortezomib resistance by inducing DNA damage and endoplasmic reticulum functional impairment in multiple myeloma.

Background: Multiple myeloma (MM) is a hematological malignancy characterized by uncontrolled proliferation of plasma cells and is currently incurable. Despite advancements in therapeutic strategies, resistance to proteasome inhibitors, particularly bortezomib (BTZ), poses a substantial challenge to disease management. This study aimed to explore the efficacy of boanmycin, a novel antitumor antibiotic, in overcoming resistance to BTZ in MM.

Association between the triglyceride to high-density lipoprotein cholesterol ratio and type 2 diabetes mellitus in non-alcoholic fatty liver disease.

This study evaluated the ability of the triglyceride (TG) to high-density lipoprotein cholesterol (TG/HDL-C) ratio to identify individuals at risk of type 2 diabetes mellitus (T2DM) in the non-alcoholic fatty liver disease (NAFLD) population. We retrospectively studied 4,769 patients with NAFLD from the Affiliated Hospital of Hangzhou Normal University (2020-2023). Binary logistic regression models were used to evaluate the association between the TG/HDL-C ratio and lipid parameters with T2DM.

Contact force measurement of the compliant gripper based on a force sensing model.

It is challenging for most existing grippers to accurately measure their contact force when grasping unstructured objects. To address this issue, a novel force sensing model is established. A compliant gripper derived by the topology optimization method is introduced, and its actual deformation is measured without contacting by OpenCV.

Electroluminescence aptasensor based on tetrahedral DNA nanostructure with exonuclease-assisted target cycling for detection of acetamiprid.

In this study, an electroluminescent (ECL) aptasensor that could efficiently and sensitively detect acetamiprid (ACE) in vegetables was constructed based on an exonuclease-assisted target cycling amplification strategy. Bimetallic RuZn-based metal-organic framework (RuZn-MOF), nucleic acid exonuclease VII (Exo VII) and tetrahedral DNA nanostructure (TDN) were used as constituent materials. First, RuZn-MOF was a substrate material with good luminescence performance and was synthesized by a hydrothermal method.

[Effects of Exogenous Substances on the Remediation of Soil Contaminated by Heavy Metals and Greenhouse Gas Emissions by × and ].

The application of exogenous substances has important effects on soil greenhouse gas emissions and phytoremediation of soil contaminated by heavy metals. × and were used as remediation plants in this study. The effects of different exogenous substances on phytoremediation and greenhouse gas emissions of heavy metal-contaminated soil were studied.

Identification of Plasma Metabolites and Dipeptides as Diagnostic Biomarkers for Psoriasis Vulgaris through Liquid Chromatography-High Resolution Mass Spectrometry-Based Metabolomics.

Psoriasis, an immune-mediated chronic inflammatory skin disease, is primarily diagnosed through clinical assessment. Currently, specific markers for the accurate diagnosis and prediction of psoriatic disease are lacking. Here, we employed a three-step designed study to perform untargeted metabolomics, with the aim of identifying candidate biomarkers for psoriasis.

Synbiotic Effects of Lacticaseibacillus paracasei K56 and Prebiotics on the Intestinal Microecology of Children with Obesity.

Lacticaseibacillus paracasei K56 (L. paracasei K56) is a probiotic with weight-loss effects. However, symbiosis research on the combined effects of Lacticaseibacillus paracasei K56 and prebiotics is lacking.

Strip biosensors based on broad-spectrum aptamers and cationic polymers for the on-site rapid detection of tetracycline antibiotics residues in milk.

A lateral flow chromatography strip (LFS) is a chromatography-based biosensor with advantages of convenient portability, simple operation and rapid detection. In this study, a novel rapid detection technique of aptamer-based chromatography strip was developed and used for the first time for the residue detection of tetracycline antibiotics (TCs) in various milk samples. In this method, gold nanoparticles (AuNPs) modified by TCs specific aptamers were used as probes, and cationic polymers as capture molecules in a test line (T-line).

5-Fluorouracil combined with CalliSphere drug-eluting beads or conventional transarterial chemoembolization for unresectable hepatocellular carcinoma: a propensity score weighting analysis.

This study aimed to assess the effectiveness and safety of 5-Fluorouracil (5-Fu) combined with conventional transarterial chemoembolization (cTACE) compared to 5-Fu combined with drug-eluting bead transarterial chemoembolization (DEB-TACE) using CalliSpheres for the treatment of unresectable hepatocellular carcinoma (HCC) using propensity score weighting methods. This retrospective analysis included 131 patients with HCC treated with 5-Fu combined with cTACE (5-Fu-cTACE group, n = 65) or DEB-TACE (5-Fu-DEB-TACE group, n = 66) at the Affiliated Hospital of North Sichuan Medical College from January 2019 to December 2022. Based on the baseline data and laboratory indicators, propensity score weighting was used to reduce confounding bias.

Novel electrochemiluminescence platform utilizing AuNPs@Uio-66-NH bridged luminescent substrates and aptamers for the detection of pesticide residues in Chinese herbal medicines.

A large number of Chinese herbal medicines (CHMs) are included in daily recipes, but their pesticide residues have aroused more and more concerns. In this paper, an electrochemiluminescence aptasensor was constructed for the trace detection of acetamiprid (ACE) in Angelica sinensis and Lycium barbarum. Possessing a large specific surface area, UiO-66 was modified with amino groups to improve biocompatibility, and the addition of AuNPs allowed UiO-66-NH to catalyze the formation of excited states of luminescent molecules (TPrA; Ru(bpy)), and AuNPs@UiO-66-NH was used to bridge the aptamer (Au-S) and luminescent substrate (peptide bond).

Absolute quantitation of human serum cystatin C: candidate reference method by N-labeled recombinant protein isotope dilution UPLC-MS/MS.

Objectives: Serum cystatin C (CysC) is a reliable and ideal endogenous marker for accurately assessing early changes in glomerular filtration rate (GFR), surpassing the limitations of creatinine-based estimated GFR. To improve the precision of GFR calculation, the development of strategies for accurately measuring serum CysC is crucial.

Methods: In this study, the full-length CysC pure product and fully recombinant N-labeled CysC internal standard were subjected to protein cleavage.

Identification of mitochondria-related genes as diagnostic biomarkers for diabetic nephropathy and their correlation with immune infiltration: New insights from bioinformatics analysis.

Background: Diabetic nephropathy (DN) is a common and severe microvascular complication of diabetes. Mitochondrial dysfunction and immune inflammation are important factors in the pathogenesis of DN. However, the specific mechanisms and their intricate interactions in DN remain unclear.