Application of Fe-CuO bimetallic oxide nanozyme-based colorimetric sensing for highly sensitive detection of tetracycline: theoretical and experimental studies.

This research establishes a competitive colorimetric detection platform for tetracycline (TC) residue analysis in milk and aqueous matrices, with implications for assessing antibiotic-derived health risks in dairy and potable water systems. According to the developed colorimetric competitive reaction model, steady-state kinetic analysis revealed that the Fe-doped CuO (Fe-CuO) nanozymes exhibit higher substrate affinity and faster reaction kinetics than conventional enzymes. The catalytic system utilized a bimetallic nanozyme composed of Fe-CuO, which exhibited enhanced catalytic performance, using 3,3',5,5'-tetramethylbenzidine (TMB) as the color-changing substrate.

Machine learning integration of bulk and single-cell RNA-seq data reveals glycolytic heterogeneity in colorectal cancer.

As one of the most prevalent malignancies worldwide, colorectal cancer (CRC) exhibits a strong metabolic dependency on glycolysis, which fuels tumor expansion and shapes an immunosuppressive microenvironment. Despite its clinical significance, the regulatory landscape and cellular diversity of glycolytic metabolism in CRC require systematic exploration. Multi-omics datasets (bulk/scRNA-seq and spatial transcriptomics) were analyzed to quantify glycolytic signatures.

Advances in microbial nitrogen metabolism: synergizing technological innovations to drive systems biology and sustainable biotechnology.

Microbial nitrogen metabolism serves as the core driver of biogeochemical cycles, governing over 90% of global nitrogen transformations through enzymatic cascades. Its core regulatory mechanisms encompass a three-tiered system-nitrogen source perception, signal transduction, and systemic network control-enabling dynamic adaptation to nitrogen fluctuations while confronting key challenges including metabolic crosstalk complexity between carbon and nitrogen pathways and energy co-integration. Recent technological breakthroughs include cryo-electron microscopy elucidating the dynamic structures of key enzymes and artificial intelligence-driven multi-omics revealing metabolic network interactions.

Intrinsic Bright and Robust Phosphorescence in Sulfur Quantum Dots Enabled by π-Conjugation Engineering and Covalent Matrix Anchoring.

Achieving efficient and stable intrinsic room-temperature phosphorescence (RTP) in sulfur quantum dots (SQDs) is vital for the advancement of metal-free afterglow materials but remains scarcely explored due to the inherent challenge of low triplet state formation efficiency. Herein, an ingenious approach is presented for constructing a phosphorescent SQDs-based system (π-SQDs-MA) by integrating π-conjugated units with covalent confinement in a metaboric acid (MA) matrix. Remarkably, π-SQDs-MA exhibits intense intrinsic green RTP with a high efficiency of 19.

Utilizing intelligent pelvic floor ultrasonography combined with real-time shear wave elastography for evaluating female pelvic floor functions.

Background: Given the high prevalence of pelvic floor disorders (PFD) and their association with structural and functional compromise of the levator ani muscle complex, particularly the puborectalis, this study aims to assess pelvic floor functionality by using intelligent pelvic floor ultrasound combined with real-time shear wave elastography (SWE) to evaluate anatomical integrity, mobility, and tissue elasticity.

Methods: Seventy-one women presenting symptoms indicative of PFD were enrolled in this study and constituted the PFD group. In contrast, an equal number of women lacking such symptoms comprised the non-PFD group.

Safety and efficacy of percutaneous radiofrequency thermocoagulation with varying puncture trajectories for recurrent maxillary trigeminal neuralgia: a prospective study.

Background: Recurrence of maxillary trigeminal neuralgia (TN) poses a significant clinical challenge. Percutaneous radiofrequency thermocoagulation (PRT) has been established as an effective treatment for maxillary TN. However, the safety and efficacy of different puncture pathways, particularly in the context of pain recurrence, may vary.

BRD4 Signaling Maintains the Differentiated State of β Cells.

In diabetes, pancreatic β cells degenerate from their mature differentiated state to a dedifferentiated state. BRD4 plays a pivotal role during embryogenesis and cancer development, but its function in modulating β-cell differentiation remains unknown. In this study, multiple models including calorie restriction db/db mouse, long-term and acute conditional knockout mouse, and human islet organoids are adopted to assess BRD4 function in β cells.

Spatiotemporal single-cell analysis elucidates the cellular and molecular dynamics of human cornea aging.

Background: The human cornea is a transparent and uniquely ordered optical-biological system. Precise coordination of its cellular mechanisms is essential to maintain its transparency and functionality. However, the spatial, cellular and molecular architecture of the human cornea and its intercellular interactions during aging have not been elucidated.

LncRNA-5829: a novel inhibitor of cardiac fibrosis.

Background: Recent studies have proved that long non-coding RNAs (lncRNAs) are closely related to the pathogenesis of cardiovascular diseases (CVDs), but their exact regulatory mechanism including non-coding as well as coding function in myocardial fibrosis need to be further explored. This study aims to explore the role of a novel and highly conserved lncRNA-5829 in myocardial fibrosis.

Methods: Thirty-two male C57BL/6 mice weighing 20-25 g (8 weeks old) were cultured under specific pathogen-free (SPF) conditions prior to the start of the experiment.

Serum neurofilament heavy chain predicts post-stroke cognitive impairment.

Serum phosphorylation neurofilament heavy chain (p-NfH) is a marker of axonal injury, and previous research has shown an association between p-NfH and both Alzheimer's disease and frontotemporal dementia. However, there have been no reports on its relationship with post-stroke cognitive impairment (PSCI). The purpose of this study is to investigate whether p-NfH can serve as a predictive biomarker for PSCI following acute ischemic stroke (AIS).

In Situ Generation by Cyclization of an Organic Structure Directing Agent for the Synthesis of High Silica Zeolite ERS-7.

Zeolites with high framework Si/Al ratios are of interest for industrial applications due to their hydrothermal stability. They are usually synthesized in the presence of pre-prepared organic structure directing agents (OSDAs). The high silica ERS-7 zeolite (topology type ESV) can be crystallized using N,N-dimethylpyrrolidinium (dmpyrr) that is formed in situ via cyclization of N,N,N',N'-tetramethyl-1,4-diaminobutane (tmdab) when a cationic polymer is also present.

Dl-3-n-Butylphthalide Promotes Neurogenesis in Ischemic Stroke Mice Through Wnt/β-Catenin Signaling Activation and Neurotrophic Factor Production.

Synchronized neurogenesis and angiogenesis after stroke have been well documented, and inducing neurovascular remodeling may provide a promising strategy to promote tissue repair and functional recovery. Dl-3-n-Butylphthalide (NBP) was reported to exert a potent angiogenic activity in rodent models of stroke. However, little is currently known regarding the effects and mechanisms of NBP on neurogenesis in ischemic stroke.

Detection and prognostic relevance of PLA2R epitopes in idiopathic membranous nephropathy: a simultaneous quantitative multiplex suspension array detection method.

Objective: This study aimed to develop a multiplex suspension assay for the simultaneous quantitative detection of anti-cysteine-rich domain (anti-CysR)/C-type lectin domain 1 (CTLD1)/C-type lectin domain 6-8 (CTLD678)-immunoglobulin G4 (IgG4) antibodies of M-type phospholipase A2 receptor (PLA2R) in the serum samples of patients to evaluate the clinical application value of PLA2R epitope spreading in disease prognosis.

Methods: CysR, CTLD1 and CTLD678 antigen domains were coupled to three types of microspheres. The optimal dilution ratio of biotinylated anti-human IgG4 was identified, and a multiplex suspension assay was evaluated in terms of linearity, sensitivity, precision, specificity and recovery rate.

The Role of Placental MFF-Mediated Mitochondrial Fission in Gestational Diabetes Mellitus.

Introduction: Gestational diabetes mellitus (GDM) refers to hyperglycemia first recognized during pregnancy, characterized by decreased insulin sensitivity and impaired glucose metabolism. Dynamic fusion and fission processes within mitochondria play critical roles in maintaining glucose metabolism homeostasis. Given the fundamental role of mitochondrial fission factor (MFF) in mitochondrial fission, the intention of this study was to investigate mitochondrial dynamics in the placentae of GDM patients and explore the role of MFF in the etiopathogenesis and progression of GDM through the modulation of glucose metabolism and insulin resistance.

Species-specific Antimony Isotope Analysis by High Performance Liquid Chromatography Coupled with Multicollector ICPMS Using Hydride Generation as an Interface.

A novel method has been developed for the simultaneous online determination of the isotopic compositions of different antimony (Sb) species in a single analytical run using high-performance liquid chromatography (HPLC) coupled with multicollector inductively coupled plasma mass spectrometry (MC-ICPMS), with hydride generation (HG) serving as the interface. Various parameters affecting the precision of Sb isotope analysis including HG conditions, transient signal processing methods and peak integration windows, were optimized. The linear regression slope method and a 100% peak integration window provided the optimal precision.

Identification of genetic loci and candidate genes regulating photosynthesis and leaf morphology through genome-wide association study in L.

Rapeseed ( L.) is a major agricultural crop with diverse applications, particularly in the production of seed oil for both culinary use and biodiesel. However, its photosynthetic efficiency, a pivotal determinant of yield, remains relatively low compared with other C plants such as rice and soybean, highlighting the necessity of identifying the genetic loci and genes regulating photosynthesis in rapeseed.

DHA Improves neurodevelopmental abnormalities in offspring of gestational diabetes mellitus patients via the PPAR-γ/FATP4 pathway.

Offspring of women with gestational diabetes mellitus (GDM) face an increased risk of long-term neurodevelopmental abnormalities. This study explores the altered expression of key placental fatty acid transport proteins-FATP2, FATP4, FATP6, FABP4, and FAT/CD36-in GDM patients, and the potential of docosahexaenoic acid (DHA) to mitigate neurodevelopmental risks in offspring by enhancing their expression through activation of peroxisome proliferator-activated receptor γ (PPAR-γ). Our findings demonstrate that placental FATP4 expression is reduced in GDM patients.

Analysis of eye movement features in patients with Alzheimer's disease based on intelligent eye movement analysis and evaluation system.

Background: The early identification of Alzheimer's disease (AD) benefits patients, so creating a simple and convenient method is crucial for diagnosing early symptoms.

Objective: To offer a potential approach for the early detection of both AD and mild cognitive impairment (MCI).

Methods: Eye movement data from 66 patients were divided into three groups, including healthy control group (HC), MCI group, and AD group.

Urine PLA2R Antibody Detection in Hazard Stratification of PLA2R-Associated Membranous Nephropathy.

Context.—: M-type phospholipase A2 receptor (PLA2R) is the major autoantigen of membranous nephropathy (MN). As the specific antibodies of MN, the correlation between serum PLA2R antibody (sPLA2R-Ab) levels and PLA2R-associated MN (PMN) risk stratification is still controversial.

A CW-Pumped Dye Nanolaser With an Optimized Nanocavity.

Plasmonic nanolasers, which are promising coherent light sources for integrated photonics, super-resolution imaging, and ultrasensitive sensing applications, face the challenge of high thresholds due to inherent losses in plasmonic nanocavities. While considerable efforts have been made to improve the Q factor, typically quantified by the full width at half maximum (FWHM), intensity (ΔI) is another critical feature of plasmonic resonance. However, the combined influence of both the Q factor and resonance intensity of a plasmonic nanocavity on nanolasing threshold has not been sufficiently explored, as experimentally controlling one variable while keeping the other constant is challenging.

Identification of mitochondria-related genes as diagnostic biomarkers for diabetic nephropathy and their correlation with immune infiltration: New insights from bioinformatics analysis.

Background: Diabetic nephropathy (DN) is a common and severe microvascular complication of diabetes. Mitochondrial dysfunction and immune inflammation are important factors in the pathogenesis of DN. However, the specific mechanisms and their intricate interactions in DN remain unclear.