A Rare Intra-Atrial Cyst of Vascular Origin: Genetic Insights From a Case.

Background: Intra-atrial cysts are rare and often mistaken for neoplastic lesions. Vascular-origin cysts are even more uncommon. Limited genetic data hamper understanding of their pathogenesis and management.

Indobufen Versus Aspirin Plus Clopidogrel in Patients After Coronary Stenting in Patients With Diabetes: A Post Hoc Analysis of the OPTION Trial.

Background: Despite increased risk of ischemic events in diabetes, the optimal anti-thrombotic strategy for secondary prevention has not been defined. We aimed to assess the efficacy and safety of optimal antiplatelet agents such as indobufen-based dual antiplatelet therapy (DAPT) in patients with diabetes after coronary stenting.

Methods: OPTION trial was a randomized, open-label, noninferiority, and multicentric study in China.

Integrative bulk and single-cell transcriptomic analysis identifies a migrasome-associated lncRNA signature predictive of prognosis and immune landscape in clear cell renal cell carcinoma.

Introduction: Clear cell renal cell carcinoma (ccRCC) is characterized by high recurrence and metastasis rates, leading to poor prognosis. Migrasomes, a class of organelles mediating intercellular communication, and long noncoding RNAs (lncRNAs) both play critical roles in tumor progression; however, the prognostic significance of migrasome-associated lncRNAs in ccRCC remains unclear.

Methods: Migrasome-associated lncRNAs were identified using The Cancer Genome Atlas (TCGA) dataset, and a prognostic risk signature was constructed.

First-line benmelstobart plus anlotinib versus sunitinib in advanced renal cell carcinoma (ETER100): a multicentre, randomised, open-label, phase 3 trial.

Background: The underexplored potential of PD-L1 blockade in advanced renal cell carcinoma highlights an urgent need for novel agents. This trial aimed to compare benmelstobart (a novel PD-L1 inhibitor) plus anlotinib with sunitinib as first-line treatment for advanced renal cell carcinoma.

Methods: ETER100 was a multicentre, randomised, open-label, phase 3 trial conducted at 37 medical sites in China.

Can intraoperative endoscopic evaluation replace postoperative low-dose CT for assessing the stone-free status in vacuum-assisted dedusting lithotripsy.

Purpose: To compare the diagnostic accuracy of intraoperative endoscopic assessment with postoperative low-dose computed tomography (LDCT) for determining stone-free status (SFS) following retrograde vacuum-assisted dedusting lithotripsy (VADL) using a tip-flexible vacuum-assisted suction ureteral access sheath (tFVS-UAS) and evaluate the feasibility of replacing routine LDCT with intraoperative evaluation.

Methods: This single-center retrospective study analyzed 1,105 patients undergoing unilateral VADL (December 2022-December 2024). All VADL procedures were performed retrograde using tFVS-UAS.

Therapeutic potential of Desmodium styracifolium polysaccharide in attenuating nano-calcium oxalate induced renal injury and fibrosis.

Calcium salt deposition in the kidney induces epithelial-to-mesenchymal transition (EMT) in renal tubular epithelial cells, which is the pathological basis for the progression to renal fibrosis in patients with renal stones; however, effective drugs to prevent and treat this disease have not been adequately investigated. In this study, we conducted a comprehensive analysis of fibrosis-related core genes by utilizing bioinformatics on RNA-seq data, along with web database information. Additionally, we designed both in vivo and in vitro experiments to elucidate the mechanisms and signaling pathways through which Desmodium styracifolium polysaccharides (Ds) mitigate renal fibrosis induced by nephrolithiasis.

Dose-optimised recombinant human thrombopoietin eltrombopag in patients with immune thrombocytopenia: a multicenter, randomised controlled trial (The TE-ITP Study).

Background: Recombinant human thrombopoietin (rhTPO) at a fixed dose of 300 U/kg/day for 2 weeks has demonstrated good efficacy and safety in adults with immune thrombocytopenia (ITP). This trial aimed to develop a flexible and personalized rhTPO regimen that ensures efficacy and safety beyond previous fixed dose, with eltrombopag as an active comparator.

Methods: The TE-ITP trial was conducted in 12 centers across China.

Modulating metabolism to improve the therapeutic outcomes of CAR cell therapies: From bench to bedside.

Chimeric antigen receptor (CAR) cell therapies have emerged as a groundbreaking approach in cancer treatment, offering new hope for patients with refractory tumors. Despite their success, the therapeutic efficacy of CAR cell therapies is often undermined by metabolic factors within the tumor microenvironment (TME), which impede CAR cell function and lead to treatment resistance. Current literature has not fully explored how these metabolic processes contribute to CAR cell therapy failure, particularly in the context of solid tumors, where the TME presents unique challenges.

Analysis of the Efficacy of Photodynamic Therapy in Treating Cervical Lesions and HPV Infections and Its Influencing Factors.

Objective: To evaluate the efficacy of photodynamic therapy (PDT) in patients with human papillomavirus (HPV) infection and cervical lesions, meanwhile factors influencing the efficacy of PDT were analyzed.

Methods: A retrospective analysis was performed on 110 patients with HPV infection and cervical lesions. All patients underwent HPV genotyping, ThinPrep Cytology Test (TCT), and pathological examination, the changes before and after PDT treatment for 6 months were analyzed.

Position-independent single-nucleotide polymorphism discrimination by CRISPR/Cas12a via rational activator strand engineering.

Single-nucleotide polymorphisms (SNPs) are critical biomarkers for disease diagnosis and genetic research, yet their sensitive and specific detection remains challenging. Here, we report a rational activator strand design strategy that significantly enhances the SNP discrimination capability of CRISPR/Cas12a-based biosensing systems. By systematically optimizing the length of the crRNA-complementary region and the architecture of the 3'-terminal random extension sequence, we developed an engineered CRISPR/Cas12a platform capable of discrimination SNPs with single-nucleotide resolution, regardless of mutation position.

Unraveling the Clinical Landscape of RNA Modification Regulators with Multi-Omics Insights in Pan-Cancer.

Cancer remains a major global health challenge, with RNA modifications increasingly recognized as key regulators of tumor progression. However, integrated pan-cancer analyses across multiple modification types are limited. We performed a comprehensive analysis of 170 RNA modification-related genes across 33 cancer types, uncovering diverse expression, mutation, and epigenetic patterns.

Elevated C-reactive protein and D-dimer to predict venous thromboembolism in patients with bladder cancer.

Objective: This study evaluated C-reactive protein (CRP) in hospitalized patients with bladder cancer (BC) and explored the predictive value of CRP for venous thromboembolism (VTE), combining CRP and D-dimer (D-D) levels to improve the ability to predict the risk of VTE in BC patients, thereby better guiding clinical prevention and treatment of this disease.

Methods: Clinical data from 4,438 patients with BC admitted between January 2015 and December 2020 were reviewed. After screening, 2,164 patients remained.

Integrating Single-Cell and Bulk RNA Sequencing Reveals the Malignant Phenotype of CBX4 in Prostate Cancer.

The expression pattern and functions of CBX4 in prostate cancers remain ambiguous. This study aims to investigate the performance of CBX4 in prostate cancer progression and preliminary inquiry potential mechanisms. The GEPIA data website was utilized to evaluate the expression patterns of CBX families and their correlations with prognosis.

Early detection of positive urine culture in patients with urolithiasis: a machine learning model with dynamic online nomogram.

Background: Positive urine cultures are common in urinary stone patients, yet tools for early infection prediction are limited. To address this gap, a user-friendly, dynamic online nomogram was developed to predict the incidence of positive urine cultures in patients with urolithiasis.

Methods: A retrospective study was conducted with 3,641 patients with urinary stones at the Second Hospital of Tianjin Medical University.

MPEP ameliorates sepsis-associated delirium by modulating hippocampal mGluR5 signaling and pyramidal neuron excitability in mice.

Background: Delirium is a cerebral manifestation commonly seen in sepsis, known as sepsis-associated delirium (SAD), and is thought to be closely related to excitatory nervous system disorders. Metabotropic glutamate receptor 5 (mGluR5) is one of the important receptors in the central glutamatergic nervous system, which is widely involved in the regulation of synaptic transmission, synaptic plasticity and excitatory/inhibitory balance. The inhibition of mGluR5 is considered as a potential drug target for reversing excitatory nervous system disorders.

A personalized prediction model for distinguishing between asymptomatic bacteriuria and symptomatic urinary tract infections in patients with type 2 diabetes mellitus using machine learning.

Background: Patients with type 2 diabetes mellitus (T2DM) have an increased susceptibility to urinary tract infections (UTIs), caused by uropathogenic (UPEC). Asymptomatic bacteriuria (ASB) is a significant contributor, but lots of patients are difficult to distinguish. Distinguishing between ASB and symptomatic UTIs can greatly assist clinicians in rational use of antimicrobials.

Increased BMD in SLD Patients Without Advanced Hepatic Fibrosis: Evidence From the NHANES 2017-2020 Database.

Recently, metabolic dysfunction-associated steatotic liver disease (MASLD) has been proposed to replace the liver condition previously known as nonalcoholic fatty liver disease (NAFLD), thereby redefining the subcategories of steatotic liver disease (SLD). However, the clinical relevance of SLD subcategories and their relationship with bone mass is lacking. In this study, we aimed to explore the potential association between the commonly proposed subclasses of fatty liver disease and bone mass.

The Predictive Value of Real-World Cardiologist Performance (RWCP) Score in Atrial Fibrillation Recurrence Risk After Radiofrequency Ablation.

Objective: To explore the predictive value of Real-world Cardiologist Performance (RWCP) score for atrial fibrillation (AF) recurrence risk after radiofrequency catheter ablation (RFCA).

Methods: Patients with nonvalvular AF who underwent first-time RFCA at Luoyang Central Hospital affiliated to Zhengzhou University between October 2021 and June 2023 were included in this study. Detailed ablation data were exported from CARTO®3 system (Biosense Webster, USA), based on which, four parameters, including discharge time proportion, Catheter Contact Force Stability (C3-FOT proportion), Ablation Index Consistency (SURPOINT/TOP-4 proportion) and fragmented points proportion, were calculated.

Latent Transition and Predictors of Cognitive Function Among Community-Dwelling Chinese Older Adults With Cardiovascular Metabolic Diseases.

Objectives: Despite the recognized heterogeneity of cognitive function in older adults and its propensity for transition, this phenomenon remains inadequately understood among older adults with cardiovascular metabolic diseases (CMD). We aimed to explore the latent profiles of cognitive function among older adults with CMD, along with the transition probabilities between profiles and associated predictors.

Methods: A cohort study including 2304 older adults with CMD was drawn from the Chinese Longitudinal Healthy Longevity Survey.

The role of three-dimensional reconstruction in partial nephrectomy for ipsilateral multifocal renal tumors: a multicenter retrospective study.

Purpose: This study aims to evaluate the clinical impact of three-dimensional (3D) reconstruction in partial nephrectomy for ipsilateral multifocal renal tumors (MIRTs), focusing on surgical precision, ischemia management, and postoperative outcomes.

Methods: A retrospective analysis was performed on 56 MIRTs patients who underwent PN at two hospitals. Patients were divided into two groups: Group A (33 patients) received standard preoperative CT scans, while Group B (23 patients) underwent 3D reconstruction.

Modified Octopus Technique for Thoracoabdominal Aortic Aneurysm.

Thoracoabdominal aortic aneurysm (TAAA) involves both the thoracic and abdominal aorta and surgical treatment requires reconstructing several important visceral branch arteries. Open surgery is considered the standard treatment for TAAA, but it carries high risks and many complications. With the advancement of endovascular techniques, endovascular repair of TAAA has gradually become a new trend in treatment.

Antimony Exposure Induces Bladder Cancer Metastasis Via Activation of the SUMOylation-Dependent TGF-β/Smad2/3 Axis.

Antimony is an important industrial raw material, known as the "industrial monosodium glutamate", and is widely used in alloys, electronics, chemical engineering, and other fields. Due to its extensive applications, its environmental toxicity and carcinogenic risk have attracted increasing attention. In recent years, numerous studies have reported the carcinogenic risk of antimony, but its role in bladder cancer and the underlying molecular mechanisms have not been fully elucidated.

5'-End Engineering of CRISPR/Cas12a Activators: A Versatile Platform for Multiple Biomarker Analysis and Clinical Cancer Tissue Identification.

The CRISPR/Cas12a system has emerged as a powerful tool for biosensing due to its unique trans-cleavage activity. However, the fundamental mechanisms governing its activation remain inadequately understood, limiting the design flexibility and application scope of CRISPR/Cas12a-based biosensors. In this study, we investigated the activation behavior of CRISPR/Cas12a, focusing on the 5'-end engineering of the activator strand.

Effect modification by life's essential 8 on the association between hearing loss and atherosclerotic cardiovascular disease among US adults.

Objective: This study aimed to evaluate whether associations between hearing loss and atherosclerotic cardiovascular disease (ASCVD) were modified by Life's Essential 8 (LE8) in US adults.

Methods: A total of 10,867 participants from five cycles of the National Health and Nutrition Examination Survey conducted between 2005 and March 2020 were included in the analysis. Multivariable logistic regression models were applied to examine the relationship between hearing loss, LE8, and ASCVD.