Antimony Exposure Induces Bladder Cancer Metastasis Via Activation of the SUMOylation-Dependent TGF-β/Smad2/3 Axis.

Antimony is an important industrial raw material, known as the "industrial monosodium glutamate", and is widely used in alloys, electronics, chemical engineering, and other fields. Due to its extensive applications, its environmental toxicity and carcinogenic risk have attracted increasing attention. In recent years, numerous studies have reported the carcinogenic risk of antimony, but its role in bladder cancer and the underlying molecular mechanisms have not been fully elucidated.

Transcription factor VBP specifically regulates granulocyte apoptosis during the immune response in oysters.

Vitellogenin-binding protein (VBP), a PAR bZIP transcription factor with homology to the cell death specification gene 2 (Ces-2) in Caenorhabditis elegans, is recognized for its role in cell apoptosis. In the present study, a VBP homolog CgVBP (also annotated as CgCes-2) containing a typical bZIP domain was identified from the Pacific oyster Crassostrea gigas. The transcript of CgVBP was predominantly expressed in haemocytes, specifically enriched within the granulocyte subpopulation.

Isolation and Molecular Characterization of Swine Parainfluenza Virus 5 from Piglets Co-Infected with PEDV.

Parainfluenza virus 5 (PIV5), now reclassified as , is a negative-sense, single-stranded RNA virus belonging to the genus of the family [...

Deciphering the multifaceted role of circular RNA in aging: from molecular mechanisms to therapeutic potentials.

Aging is an inevitable physiological process that occurs in living organisms and has significant implications for health and disease. As the human lifespan extends, the functionality of organs gradually diminishes, leading to the emergence of various aging-related symptoms. While it is not feasible to completely halt the aging process, investigating key molecules involved in aging can help devise valid strategies to delay its progression.

The whole genome analysis of the wild-type and attenuated orf virus reveals that ORF022 facilitates viral replication.

Background: Contagious ecthyma is an acute infectious zoonosis caused by orf virus (ORFV). Live-attenuated ORFV vaccines have played a crucial role in preventing contagious ecthyma for decades. However, these vaccines often fail to induce long-lasting immunity.

Optimized bacterial consortium-based strategies for bioremediation of PAHs-contaminated soils: insights into microbial communities, and functional responses.

Microbial technologies hold great promise for in situ remediation of polycyclic aromatic hydrocarbons (PAHs)-contaminated soils. However, the selection of enhancement measures and corresponding remediation strategies remains insufficiently understood. In this study, a series of enhancement treatments, including bacterial consortium inoculation (comprising Achromobacter denitrificans BP1, Rhodococcus aetherivorans BW2, and Lysinibacillus sp.

Study of Different Gas Floodings on Minimum Miscibility Pressure and Oil Recovery in a Low-Permeability Reservoir with Medium Viscous Oil.

Although gas flooding has been successfully applied for enhanced oil recovery throughout the world, most of its applications are conducted in low, ultralow, and tight oil reservoirs with light oil. Studies focused on a low-permeability reservoir with medium viscous oil are rarely reported, and there is little experience in the development of this type of reservoir for reference. Based on the special characteristics of low-permeability formation coupled with medium viscous oil in Niuquanhu Block, China, the gas-oil interactions of different gases are first evaluated through the pressure-volume-temperature (PVT) analysis.

Remediation of toluene-contaminated soils by sequential treatment: Soil vapor extraction systems and internal combustion engine units.

This paper reports soil vapor extraction (SVE) systems and internal combustion engine (ICE) units to remediate soils contaminated with toluene. Response surface methodology (RSM) was used to evaluate the influence of toluene concentration, air flow rate and soil water content on SVE systems, thus to identify the optimal conditions for SVE systems. ICE units were used to treat the SVE off-gas extracted from toluene-contaminated soils, and the performance in removing toluene was effectively evaluated.

Functional analysis of retinal-binding protein in the visual system of the nocturnal marine shellfish Pacific abalone (Haliotis discus hannai).

The Pacific abalone (Haliotis discus hannai) is a nocturnal species that inhabits marine benthic ecosystems, characterized by its aversion to light and preference for darkness during daylight hours, with movement and feeding predominantly occurring at night. However, the morphology and structure of its visual organs and the mechanisms underlying its perception of cyclical light variations are unknown. This study analyzed the Retinal-binding protein (RALBP) gene to elucidate its role in the abalone's visual system.

Enhancing humification in high-temperature composting: Insights from endogenous and exogenous heating strategies.

Livestock manure is difficult to manage for high moisture and nutrients. High-temperature composting (> 75 °C) reduces moisture. However, the humification process, crucial for nutrient recycling, remains poorly understood.

A novel pigeon paramyxovirus type 1 isolated from a sick racing pigeon in the Qinghai-Tibet Plateau of China shows high virulence in chickens.

Pigeon paramyxovirus type-1 (PPMV-1) is the causative agent of pigeon Newcastle disease (ND), which has caused huge losses to the pigeon industry. In this study, a PPMV-1 strain, PPMV-1/QH-01/CH/23, was isolated from a sick racing pigeon in the Qinghai-Tibet Plateau, China in 2023. The mean death time of chicken embryos and the intracerebral pathogenicity index (ICPI) were 76.

Comparative RNA sequencing analysis of three Capripoxvirus infections in an immortalized hTERT-bOEC cell model.

Capripoxviruses (CaPVs), such as lumpy skin disease, sheep pox, and goat pox, cause significant production and economic losses and are major constraints to the growth of livestock production in endemic areas. Understanding the pathogenic mechanism of CaPVs and their translation into clinical applications depends on the availability of a suitable cell line. In this study, we used a lentiviral packaging system to establish an immortalized hTERT-bOEC cell line by ectopic introduction of human telomerase reverse transcriptase (hTERT).

Effect of biosurfactants on the transport of polyethylene microplastics in saturated porous media.

Microplastic (MP) pollution has become a significant global environmental issue, and the potential application of biosurfactants in soil remediation has attracted considerable attention. However, the effects of biosurfactants on the transport and environmental risks of MPs are not fully understood. This study investigated the transport of polyethylene (PE) in the presence of two types of biosurfactants: typical anionic biosurfactant (rhamnolipids) and non-ionic biosurfactant (sophorolipids) using column experiments.

Inflammation-free electrochemical in vivo sensing of dopamine with atomic-level engineered antioxidative single-atom catalyst.

Electrochemical methods with tissue-implantable microelectrodes provide an excellent platform for real-time monitoring the neurochemical dynamics in vivo due to their superior spatiotemporal resolution and high selectivity and sensitivity. Nevertheless, electrode implantation inevitably damages the brain tissue, upregulates reactive oxygen species level, and triggers neuroinflammatory response, resulting in unreliable quantification of neurochemical events. Herein, we report a multifunctional sensing platform for inflammation-free in vivo analysis with atomic-level engineered Fe single-atom catalyst that functions as both single-atom nanozyme with antioxidative activity and electrode material for dopamine oxidation.

Allosteric inhibition of CFTR gating by CFTRinh-172 binding in the pore.

Loss-of-function mutations of the CFTR gene cause the life-shortening genetic disease cystic fibrosis (CF), whereas overactivity of CFTR may lead to secretory diarrhea and polycystic kidney disease. While effective drugs targeting the CFTR protein have been developed for the treatment of CF, little progress has been made for diseases caused by hyper-activated CFTR. Here, we solve the cryo-EM structure of CFTR in complex with CFTRinh-172 (Inh-172), a CFTR gating inhibitor with promising potency and efficacy.

Structure and function of vasa gene in gonadal gametogenesis of Pacific abalone.

Pacific abalone (Haliotis discus hannai) is a marine gastropod mollusc with significant economic importance in both global fisheries and aquaculture. However, studies exploring the gonadal development and regulatory mechanisms of Haliotis discus hannai are limited. This study aimed to explore whether the vasa gene acted as a molecular marker for germ cells.

Platform establishment of the Cre-loxP recombination system for genetic manipulation of the Lumpy skin disease virus.

Lumpy skin disease virus (LSDV) is a rapidly emerging pathogen in Asia, including China. Genetic manipulation of the LSDV is essential for the elucidation of the pathogenic mechanism and biological function of the LSDV-encoded protein. In this study, we established a platform for the Cre-loxP recombination system under a modified early-late H5 promoter of the VACV for quick construction of the recombinant LSDV virus.

FeO-Cy5.5-trastuzumab magnetic nanoparticles for magnetic resonance/near-infrared imaging targeting HER2 in breast cancer.

This study developed a probe FeO-Cy5.5-trastuzumab with fluorescence and magnetic resonance imaging functions that can target breast cancer with high HER2 expression, aiming to provide a new theoretical method for the diagnosis of early breast cancer. FeO-Cy5.

Identification of the murine osteoblastic cell MC3T3-E1 as a permissive cell line in response to lumpy skin disease virus.

Lumpy skin disease virus (LSDV) is a rapidly emerging pathogen in China. Screening suitable cells for LSDV replication is vital for future research on pathogenic mechanisms and vaccine development. Previous comparative studies have identified that the rodent-derived BHK21 is a highly susceptible cell model to LSDV infection.

Laboratory Experiments of Hydrocarbon Gas Flooding and Its Influencing Factors on Oil Recovery in a Low Permeability Reservoir with Medium Viscous Oil.

In view of the problems of low liquid production, a high proportion of high water cut wells, and poor development effect in the late stage of water flooding in the special sandstone reservoir of Niuquanhu "low permeability and medium viscosity crude oil", we carried out the research on hydrocarbon gas oil recovery and its influencing factors. First, the influence of different injected gas media on the physical properties of crude oil was analyzed. Second, the core displacement experiments of different gas injection media including CO, CH, and hydrocarbon gas were carried out by using the method of oil recovery comparison and optimization.

A nomogram based on MRI radiomics features of mesorectal fat for diagnosing T2- and T3-stage rectal cancer.

Purpose: To develop and validate a nomogram for the preoperative diagnosis of T2 and T3 stage rectal cancer using MRI radiomics features of mesorectal fat.

Methods: The data of 288 patients with T2 and T3 stage rectal cancer were retrospectively collected. Radiomics features were extracted from the lesion region of interest (ROI) in the MRI high-resolution T2WI, apparent diffusion coefficient (ADC), and diffusion-weighted imaging (DWI) sequences.

Comparative Responses of Orange-Foot and Common-Foot to Carotenoid-Enriched Diets: Survival, Heat Tolerance, and Bacterial Resistance.

Carotenoids, known to enhance survival, heat tolerance, and bacterial resistance, play an essential role in the nutrition of economically important aquatic animals. This study specifically examined their impact as feed additives on the abalone . We prepared 13 compound feeds with varying levels of astaxanthin, zeaxanthin, and -carotene, and administered them to both common-footed and orange-footed .