All-In-One Iontronic Sensing Aligner for High-Precision 3D Orthodontic Force Monitoring.

Clear aligners offer aesthetic and comfort advantages in orthodontics, yet their ability to deliver effective forces relies heavily on empirical judgment or large-scale optical scanning, lacking real-time quantitative evaluation. Integrating pressure sensors into aligners is a promising solution, but challenges in miniaturization, multi-dimensional sensing, measurement accuracy, and biocompatibility hinder clinical application. Here, an all-in-one Orthodontic Force Acquisition System (OFAS) is presented that enables real-time, 3D force monitoring using a cross-shaped iontronic sensing array and an origami-inspired, wireless battery-free readout circuit miniaturized for single-tooth placement.

Promotion of Biomimetic Mineralization Preinfiltration of Mineral Precursors.

Intrafibrillar mineralization plays an important role in dentin repair. Current research on intrafibrillar mineralization primarily focuses on the precise positioning of mineral precursors within collagen and the reduction of mineralization time. Inspired by the multifunctionality of noncollagenous proteins (NCPs), we developed a dual-analogue system, 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP) followed by 3-(3,4-dihydroxyphenyl)-l-alanine (l-DOPA)-stabilized amorphous calcium phosphate (M + LA), which integrated a nucleation inhibitor l-DOPA and inducer 10-MDP.

Categories, applications, and potential of stem cells in bone regeneration: an overview.

Bone defects affect many individuals globally and can result in significant suffering and impairment, particularly among the elderly population. In addition, current treatment options for critical-size bone defects, such as autologous or allogeneic bone graft transplantation, present significant challenges. Within this clinical scenario the identification of novel and effective approaches for bone regeneration is urgently needed, and options derived from tissue engineering may be particularly appealing.

ROS and Drp1-mediated mitochondrial fission contributes to the hyposalivation caused by Sjögren's disease.

Background: Sjögren's disease (SjD) is an autoimmune disorder characterized by sicca syndrome with undetermined roles of reactive oxygen species (ROS) and mitochondrial dynamics in the damaged glands. This study aimed to clarify the roles of ROS and mitochondrial dynamics in SjD-related hyposalivation and explored the therapeutic effect of targeting mitochondrial fission to treat hyposalivation in murine SjD.

Methods: Single-cell sequencing analysis of submandibular glands (SMGs) from mice with SjD was performed.

Graft Dimensions and Residual Tissue Thickness Impact on Donor Site Healing After FGG Procedure: A 3D Comparative Study.

Introduction And Aims: Free gingival grafting (FGG) is a common procedure in periodontal and implant therapy to augment keratinized tissue and improve esthetic outcomes. However, the effects of graft dimensions and residual tissue thickness (RTT) on palatal donor site healing remain poorly characterized, lacking standardized 3-dimensional analysis. This study aimed to quantify how these factors influence 3-dimensional tissue recovery over 6 months, with thickness recovery rate as the primary outcome.

[The significance and clinical applications of periodontal chart: part IV of a series on basic training in periodontal diagnosis and treatment].

The periodontal chart comprehensively and objectively records the periodontal status of each tooth in the entire dentition, which is an important basis and essential for the correct diagnosis of periodontal diseases and treatment plan formulation. This article describes in detail the content and clinical significance of the periodontal chart, the key points of the implementation of the clinical completion of the periodontal chart and the step-by-step training mode. This article, as the fourth chapter in the basic skills training series for periodontal diagnosis and treatment, aims to provide guidance for the standardization of periodontal chart recording and application in the clinic and teaching, so as to promote the standardization of Chinese periodontal diagnosis and treatment and bring it on par with the international advanced level.

[Digital design and manufacturing method of double constrained split guide for orthodontic miniscrew implantation].

This study explored a novel digital design and fabrication method for a double constrained split orthodontic miniscrew guide to improve the accuracy and safety of clinical miniscrew implantation and reduce related complications. A patient requiring miniscrew implantation was selected, and data were acquired using cone beam computed tomography (CBCT) and intraoral optical scanning. For the construction of a double constrained split guide including a screw-hole guide and an insertion rod guide, different types of software such as Mimics 24.

Oral virome metagenomic catalog links Porphyromonas gingivalis phages to obesity and type 2 diabetes.

The human microbiota has a critical role in maintaining human microbiome homeostasis and health, yet the viral component of the oral microbiome remains largely unidentified. We establish the Human Oral Virome Database (HOVD) catalog, a freely accessible online resource cataloging 24,440 bacteriophage viral operational taxonomic units and 83 eukaryotic viral genomes. Utilizing HOVD, we investigate oral virome variation and its correlation with oral bacteria and gut virome in 220 obese individuals with or without type 2 diabetes mellitus (T2D).

Three-dimensional cell sheet model improves prediction accuracy of osteogenic potential for biodegradable magnesium-based metals.

Biodegradable metals have been increasingly utilized clinically due to their biosafety and pro-osteogenic properties. However, conventional monolayer cell-based preclinical safety evaluation methods based on ISO10993-5 consistently indicate significant cytotoxicity that contradicts outcomes. In this study, we aimed to establish an evaluation model that better correlates with performance.

Genotype-Phenotype Correlation in Fibrous Dysplasia/McCune-Albright Syndrome Patients With Craniofacial Lesions.

Objectives: We aimed to investigate genotype-phenotype correlations, variant prevalence, and prognostic factors in a Chinese cohort with craniofacial FD/MAS.

Methods: A retrospective study of 93 histologically confirmed FD/MAS cases (2003-2024) analyzed GNAS mutations via direct sequencing. Clinical data, including disease onset, lesion activity (active vs.

Evaluating Temporomandibular Disorder Severity: A Four-Dimensional Model.

Background: This study developed and tested a multi-dimensional model for evaluating the severity of temporomandibular disorders (TMDs) based on the five core symptoms (5Ts) of the Diagnostic Criteria for TMDs (DC/TMD).

Methods: Study participants were sourced from a university hospital. The 5Ts screener was expanded to include the dimensions of duration, frequency, intensity, and interference with daily activities (5Ts-4D).

Efficient Synthesis of Protein-Polymer Conjugates with Open-Air Fabrication and Facile Purification Driven by the Thermoresponsive Protein-Polymer Conjugate.

A simple, efficient, and broadly applicable method for the preparation of protein-polymer conjugates is of critical importance for their further development. However, existing approaches are often limited by challenges such as insufficient deoxygenation, complex purification processes, and poor compatibility of monomers and proteins. Herein, we present a comprehensive strategy for the synthesis of protein-polymer conjugates utilizing a thermosensitive, recyclable glucose oxidase-poly-(di-(ethylene glycol) methyl ether methacrylate) conjugate (GOX-PDEGMA) as part of the deoxygenation system.

Clinical efficacy and safety of bipolar water as a therapeutic approach for oral candidiasis: a randomized controlled trial.

Objectives: Oral candidiasis (OC) is a fungal infection caused primarily by Candida albicans. Treatments are associated with side-effects and toxicity. Bipolar water (BW), a novel functional water with antimicrobial, biofilm-disrupting, and wound-healing properties, offers a promising alternative therapy.

GDC-0449 suppresses odontogenic keratocyst aggressiveness in fibroblasts by upregulating SPARC via Hedgehog pathway inhibition.

Objective: Odontogenic keratocysts (OKCs) are aggressive jaw lesions, either linked to Gorlin-Goltz syndrome or sporadic. We aimed to investigate the impact of the Hedgehog (Hh) pathway inhibitor GDC-0449 on OKC fibroblasts (OKC-Fs). Additionally, we explored whether secreted protein acidic and rich in cysteine (SPARC) was a novel Hh target and its association with OKC aggressiveness.

An energy metabolism-engaged nanomedicine maintains mitochondrial homeostasis to alleviate cellular ageing.

Energy restriction is closely related to cellular senescence and species longevity. Here, based on the structure and function of ATP synthase, a key enzyme for energy generation, we develop energy metabolism-engaged nanomedicines (EM-eNMs) to rejuvenate aged stromal/stem cells, and help to prevent skeletal ageing. We show that EM-eNMs infiltrate the mitochondria of aged bone marrow mesenchymal stromal/stem cells (BMMSCs), driving mitochondrial fission, mitophagy, glycolysis and maintaining BMMSC stemness and multifunction.

Current applications and future perspectives on rare-earth-based materials in stomatology.

A confluence of factors, including the incompatibility of conventional materials with the oral environment, inadequate dynamic response, and insufficient multi-objective synergy, hinders precise diagnosis and efficient treatment in contemporary stomatology. Due to their unique electronic configuration and multivalent properties, rare earth elements (REEs) have shown excellent optical response, enzyme simulation activity, antibacterial efficacy, and biological regulation potential. These attributes offer a promising path for developing a new generation of oral functional materials.

Extracellular Vesicles of Salivary Adenoid Cystic Carcinoma Suppress Immune Microenvironment in Lung.

Objective: Salivary adenoid cystic carcinoma (SACC) frequently metastasizes to the lung and affects patient survival. Extracellular vesicles (EVs) have significant effects in the tumor pre-metastatic niche (PMN). In this study, we aimed to investigate immune changes in the lung pre-metastatic microenvironment of SACC.

Three-dimensional reconstruction based on computed tomography/magnetic resonance imaging multimodal image fusion for parotid gland tumor diagnosis and treatment.

This research evaluated a novel computed tomography (CT)/magnetic resonance imaging (MRI) fusion-based three-dimensional (3D) reconstruction method for parotid gland tumor diagnosis and treatment. In 11 patients with primary parotid tumors adjacent to the facial nerve, contrast-enhanced CT and 3D multi-echo in steady acquisition MRI images were automatically fused, segmented, and reconstructed to visualize tumors and critical anatomical structures. Preoperative tumor localization and tumor-nerve relationship assessments matched intraoperative findings in 90.

Eosinophilic Sialodochitis: Histopathological Characteristics and Treatment Outcomes.

Objectives: To evaluate the traditional histopathology, expression of allergy-related cytokines, and treatment results of eosinophilic sialodochitis (ES).

Methods: Fifty-four patients with suspected ES and six patients with chronic obstructive sialadenitis (COS) were prospectively enrolled. Terminal duct biopsy of the affected glands was performed concomitantly with endoscopy-assisted duct dilatation.

Application of sialography in diagnosis and differential diagnosis of IgG4-related sialadenitis.

Objectives: This study aimed to identify imaging features of IgG4-related sialadenitis (IgG4-RS) and differentiate it from Sjögren's syndrome (SS) and chronic obstructive parotid/submandibular sialadenitis (COP/COS).

Study Design: A retrospective analysis of sialographic findings was conducted on 40 IgG4-RS patients. Two radiologists blindly evaluated sialographic features, including ductal dilation, wall smoothness, duct interruption, acinar patterns, and glandular emptying.

Fabrication of Site-Specific 3D Structures via Macroscopic Supramolecular Assembly for Spatially Controlled Alignment of Multiple Cells.

The self-assembly of micrometer-to-millimeter components, referred to as "macroscopic supramolecular assembly (MSA)," offers an efficient approach for constructing cell-scale 3D bioactive structures with flexible modular designs. Compared with available 3D bio-printing or conventional modular assembly of cell-material units, MSA is advantageous in decoupling material preparation and cell loading processes by directing cell adhesion after the preparation of 3D structures, which minimizes the trade-off between cell viability and material selection. But the challenge lies in efficient self-sorting of different cells and spatially controlled cell distribution.

Inactivated Bacillus subtilis R0179 Inhibits Porphyromonas Gingivalis-Induced Gingival Inflammation Via TLR2/NF-κB Signaling in a Murine Model of Periodontitis.

This study aims to explore the mechanism by which heat-inactivated Bacillus subtilis (B. subtilis) R0179 modulates the TLR2/NF-κB signaling pathway to inhibit Porphyromonas gingivalis (P. gingivalis) induced gingival inflammation.

Nanozymes in Oral Medicine: From Catalytic Design to Advanced Dental Therapies.

Nanozymes have garnered increasing attention in oral healthcare due to their enzyme-mimetic properties and potential to tackle challenges associated with oral diseases. These nanomaterials exhibit environmentally responsive modulation of reactive oxygen species (ROS) levels, enabling them to generate ROS to eradicate bacterial biofilms and drug-resistant pathogens during infection, while scavenging ROS to reduce inflammation and enhance tissue regeneration in restorative contexts. Recent advancements in rational nanozyme design, including surface modifications, elemental doping, artificial intelligence-driven optimization, and biomimetic engineering, have significantly enhanced catalytic efficiency, stability, and precision in targeting specific sites.