Three-dimensional cell sheet model improves prediction accuracy of osteogenic potential for biodegradable magnesium-based metals.

Biodegradable metals have been increasingly utilized clinically due to their biosafety and pro-osteogenic properties. However, conventional monolayer cell-based preclinical safety evaluation methods based on ISO10993-5 consistently indicate significant cytotoxicity that contradicts outcomes. In this study, we aimed to establish an evaluation model that better correlates with performance.

Effectiveness of telerehabilitation in postoperative outcomes in patients on hip fracture surgery: a meta-analysis of randomized controlled trials.

Objective: To comprehensively assess the rehabilitation efficacy of telerehabilitation compared with other traditional rehabilitation therapies in postoperative hip fracture patients.

Data Sources: Seven electronic databases: PubMed, Embase, The Cochrane Library, Web of Science, CNKI, Wan Fang, and VIP Databases, were searched from inception until October 2023.

Study Selection: Two independent reviewers selected randomized controlled trials (RCTs) that assessed the efficacy of telerehabilitation intervention approach to postoperative hip fracture rehabilitation.

Genome-wide identification of gene family members in soybean and expression analysis in response to biotic and abiotic stresses.

(), a key regulator in plant defense responses, plays central roles in resistance to stresses. Therefore, the identification and characterization of soybean family genes and verification of how these genes are associated with stresses are the focus of this study. We identified 11 genes, which all have lipase-like and EP (EDS1-PAD4-specific) conserved domains, they are unevenly distributed across six chromosomes, including tandem repetitions.

Dual-mode sensors based on double enzyme-mimicking MnCu nanoflowers as fluorescence probe for the detection of organophosphorus pesticides and 2,4-dichlorophenol.

Organophosphorus pesticides (OPs) and 2,4-dichlorophenol (2,4-DP) are widely used, but their residues have brought serious threatens to environment and human health as environmental pollutants. We synthesized double enzyme-mimicking and multifunctional MnCu flower-like nanoenzymes (NH-MnCu NFs) with fluorescence properties, oxidase (OXD)-like activity and laccase-like activity. NH-MnCu NFs exhibit excitation-independent emission fluorescence properties, as well as excellent OXD-like activity (K = 0.

Inhibition of Drp1-mediated mitochondrial fission by P110 ameliorates renal injury in diabetic nephropathy.

Diabetic nephropathy (DN) is a leading cause of end-stage renal disease, characterized by progressive renal injury driven by mitochondrial dysfunction and metabolic reprogramming. Excessive mitochondrial fission, mediated by dynamin-related protein 1 (Drp1), contributes to mitochondrial fragmentation and cellular injury in the diabetic kidney. Here, we investigate the therapeutic potential of P110, a selective inhibitor of Drp1-mediated mitochondrial fission, in experimental models of DN.

The role of Hox genes in shaping embryonic external morphology of the primitive insect Thermobia domestica (Zygentoma).

Insects represent one of the most evolutionarily successful groups, with their diversity hypothesized to be related to the regulatory roles of Hox genes, a set of related genes encoding homeodomain transcription factors determining the identity of segments along the anterior-posterior axis of the embryo. However, functional insights into the roles of Hox genes in primitive ametabolous insects, which represent the critical transition from aquatic crustaceans to winged insects, have been limited. In this study, we identified complete protein-coding sequences of 10 Hox genes in the Zygentoma Thermobia domestica, and applied clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated nuclease 9 (Cas 9) mediated gene knockout (KO) to decipher their functions.

A biodegradable magnesium alloy promotes subperiosteal osteogenesis via interleukin-10-dependent macrophage immunomodulation.

In situ bone regeneration and vertical bone augmentation have been huge problems in clinical practice, always imposing a significant economic burden and causing patient suffering. Herein, MgZnYNd magnesium alloy rod implantation in mouse femur resulted in substantial subperiosteal new bone formation, with osteoimmunomodulation playing a pivotal role. Abundant macrophages were attracted to the subperiosteal new bone region and proved to be the most important regulation cells for bone regeneration.

Caloric restriction exacerbates renal post-ischemic injury and fibrosis by modulating mTORC1 signaling and autophagy.

Objective: This study investigates the effects of caloric restriction (CR) on renal injury and fibrosis following ischemia-reperfusion injury (IRI), with a focus on the roles of the mechanistic/mammalian target of rapamycin complex 1 (mTORC1) signaling and autophagy.

Methods: A mouse model of unilateral IRI with or without CR was used. Renal function was assessed through serum creatinine and blood urea nitrogen levels, while histological analysis and molecular assays evaluated tubular injury, fibrosis, mTORC1 signaling, and autophagy activation.

Molecular basis of E93-dependent tissue morphogenesis and histolysis during insect metamorphosis.

The evolution of insect metamorphosis has profoundly influenced their successful adaptation and diversification. Two key physiological processes during insect metamorphosis are notable: wing maturation and prothoracic gland (PG) histolysis. The ecdysone-induced protein 93 (E93) is a transcription factor indispensable for metamorphosis.

Juvenile hormone signaling is indispensable for late embryogenesis in ametabolous and hemimetabolous insects.

Background: Juvenile hormone (JH) is an insect-exclusive hormone involved in regulating diverse aspects of insect physiology, and the evolution of its diverse function is widely interesting. Studying embryogenesis in basal wingless insects is important to understand the functional evolution of JH; however, experimental studies in this regard are scarce. In this study, we conducted CRISPR/Cas9-mediated knockout (KO) of genes involved in JH biosynthesis and signaling cascades in the ametabolous firebrat, Thermobia domestica.

Optimal tooth sectioning using a surgical handpiece and elevator: a finite element study of horizontally deeply impacted mandibular third molars.

Objectives: To conduct a finite element analysis of the impact of different variables on tooth sectioning efficiency and trauma to surrounding tissues when utilizing high-speed surgical handpieces and elevators.

Methods: CBCT data from the horizontally impacted third mandibular molar (M3M) of a patient were utilized to establish digital models of the M3M, adjacent M2M, and surrounding bone. To simulate tooth sectioning, a 3D finite element model was established with the following variables: remaining tooth tissue thickness (1-5 mm), tooth section fissure width (1-3 mm), elevator depth in fissure (2-6 mm), elevator position (buccal, lingual, central), elevator width (2-5 mm), and application of force (rotating, levering).

Effect of mineralized dentin matrix on the prognosis of bone defect and retained root after coronectomy.

Objective: To investigate the impact of mineralized dentin matrix (MDM) on the prognosis on bone regeneration and migration of retained roots after coronectomy.

Materials And Methods: Patients were divided into three groups based on the type of bone graft after coronectomy: Group C (n = 20, collagen), Group T (n = 20, tricalcium phosphate (TCP) + collagen), and Group D (n = 20, MDM + collagen). CBCT scans, conducted immediately and 6 months after surgery, were analyzed using digital software.

Effect of tissue fixatives on the corrosion of biomedical magnesium alloys.

In this work, the corrosion behavior of pure Mg, Mg3Ag, Mg6Ag, and MgZnYNd alloys in different fixatives (ethyl alcohol (EA), 85 % ethyl alcohol (85 % EA), 10 % neutral buffered formalin (10 % NBF), 4 % glutaric dialdehyde (4 % GD), and 4 % paraformaldehyde (4 % PFA)) was investigated to provide a valuable reference for the selection of fixatives during the histological evaluation of Mg implants. Through the hydrogen evolution test, pH test, and corrosion morphology and product characterization, it was found that corrosion proceeded slowest in the EA and 85 % EA groups, slightly faster in 4 % GD, faster in 10 % NBF, and fastest in 4 % PFA. After corrosion, the EA group surface remained unchanged, while the 85%EA group surface developed minor cracks and warping.

E93 is indispensable for reproduction in ametabolous and hemimetabolous insects.

Ecdysone-induced protein 93 (E93), known as the 'adult-specifier' transcription factor in insects, triggers metamorphosis in both hemimetabolous and holometabolous insects. Although E93 is conserved in ametabolous insects, its spatiotemporal expression and physiological function remain poorly understood. In this study, we first discover that, in the ametabolous firebrat Thermobia domestica, the previtellogenic ovary exhibits cyclically high E93 expression, and E93 mRNA is broadly distributed in previtellogenic ovarioles.

Combating land degradation through human efforts: Ongoing challenges for sustainable development of global drylands.

Drylands, as highly vulnerable ecosystems, support environmental functions and human well-being. Nevertheless, widespread land degradation and desertification present significant global and regional environmental challenges, with limited consensus on their area and degree. This study used time-series vegetation productivity and meteorological data from 2000 to 2020 to quantify global land degradation trends and driving factors in drylands.

Isolation and characterizations of multidrug-resistant human cancer cells by a biodegradable nano-sensor.

Multidrug resistance (MDR) remains a significant challenge in cancer therapy, with inherent and acquired resistance distinct. While conventional drug selection processes enable the isolation of cancer cells with acquired multidrug resistance, identifying cancer cells with inherent drug resistance remains challenging. Herein, we proposed a molecular beacon (MB)-based strategy to identify and isolate the inherent MDR cancer cells.

Accuracy of computer-assisted dynamic navigation when performing coronectomy of the mandibular third molar: A pilot study.

Objectives: The study represents a preliminary evaluation of the accuracy of the dynamic navigation system (DNS) in coronectomy of the mandibular third molar (M3M).

Methods: The study included participants with an impacted M3M near the inferior alveolar canal. The coronectomy planes were designed before the surgery using cone-beam computed tomography (CBCT) imaging data and then loaded into the DNS program.

Long-Term Temporospatial Complementary Relationship between Degradation and Bone Regeneration of Mg-Al Alloy.

The utilization of guided tissue regeneration membranes is a significant approach for enhancing bone tissue growth in areas with bone defects. Biodegradable magnesium alloys are increasingly being used as guided tissue regeneration membranes due to their outstanding osteogenic properties. However, the degradation rates of magnesium alloy bone implants documented in the literature tend to be rapid.

A Novel Joint Channel Estimation and Symbol Detection Receiver for Orthogonal Time Frequency Space in Vehicular Networks.

A vehicular network embodies a specialized variant of wireless network systems, characterized by its capability to facilitate inter-vehicular communication and connectivity with the encompassing infrastructure. With the rapid development of wireless communication technology, high-speed and reliable communication has become increasingly important in vehicular networks. It has been demonstrated that orthogonal time frequency space (OTFS) modulation proves effective in addressing the challenges posed by high-mobility environments, as it transforms the time-varying channels into the delay-Doppler domain.

Simplifying the complexity: Single enzyme (choline oxidase) inhibition-based biosensor with dual-readout method for organophosphorus pesticide detection.

Organophosphorus pesticides (OPs) are widely used in agricultural production, but their residues could cause pollution to the environment and living organisms. In this paper, a simple dual-readout method for OPs detection was proposed based on ChOx single enzyme inhibition. Firstly, ChOx can catalyze the production of HO from choline chloride (Ch-Cl).

Endoscopic visualization of the inferior alveolar nerve associated with somatosensory changes after impacted mandibular third molar extraction.

The aim of this study is to assess the relationship between somatosensory functional changes and inferior alveolar nerve (IAN) exposure after impacted mandibular third molars (M3M) removal. We recruited 35 patients who underwent impacted M3M extraction near the IAN. The M3Ms were extracted by combined endoscopy, piezosurgery, and contra-angle high-speed turbine handpiece.

Radionuclide I-labeled albumin-indocyanine green nanoparticles for synergistic combined radio-photothermal therapy of anaplastic thyroid cancer.

Objectives: Anaplastic thyroid cancer (ATC) cells cannot retain the radionuclide iodine 131 (I) for treatment due to the inability to uptake iodine. This study investigated the feasibility of combining radionuclides with photothermal agents in the diagnosis and treatment of ATC.

Methods: I was labeled on human serum albumin (HSA) by the standard chloramine T method.

HSA-MnO-I Combined Imaging and Treatment of Anaplastic Thyroid Carcinoma.

Compelling evidence suggests that nanoparticles (NPs) play a crucial role in cancer therapy. NPs templated with human serum albumin (HSA) has good retention in tumors. Manganese dioxide (MnO) has been used to enhance the effect of radiotherapy.