Synthesis and characterization of anthocyanin-loaded bovine serum albumin nanoparticles: unveiling the encapsulation mechanisms with computational insights.

Background: Black rice anthocyanins (BRA) offer significant health benefits but are limited in application due to their low bioavailability. Bovine serum albumin (BSA) nanoparticles (NPs) have been shown to effectively enhance the stability of encapsulated BRA. However, the mechanism of BRA-BSA NP formation and their molecular interactions remain unclear.

Transcriptomic Analysis Reveals Patterns of Expression of Stage-Specific Genes in Early Embryos.

Background/objectives: development is described as comprising four stages: embryo, larva, pupa, and adult. There are significant differences between workers and drones in terms of physiological functions and social roles, and the formation of the organ primordia occurs during the embryonic stage. Therefore, the objective of this study is to investigate the differential expression of and alternative splicing of genes in worker and drone embryos and to explain their unique developmental patterns.

An Evaluation of Morphometric Characteristics of Honey Bee () Populations in the Qinghai-Tibet Plateau in China.

, a native type of honey bee in China, adapts well to the Qinghai-Tibet Plateau (QTP) environments with high altitude, cold, low oxygen, and strong radiation. In this study, we detected 40 morphological characteristics from 100 colonies in 49 regions. We not only evaluated the morphometric characteristics of honey bee populations in the QTP but also found that the pigmentation of labrum and tergite 2 in is significantly different from that in .

Early life imidacloprid and copper exposure affects the gut microbiome, metabolism, and learning ability of honey bees (Apis mellifera).

The pesticide imidacloprid and the heavy metal copper provide some degree of protection to plants, while at the same time causing varying degrees of damage to bees. However, few studies have investigated the negative effects of imidacloprid and copper exposure on newly emerged bees (young bees), especially when both are present in a mix. In this study, young bees were exposed to sterile sucrose solutions containing imidacloprid (10 μg/L, 100 μg/L), copper (10 mg/L, 50 mg/L), or a mix of both (10 μg/L + 10 mg/L) for 5 days to assess their gut system and behavior, with survival and dietary consumption recorded over 21 days.

An Integrated Virtual Screening Platform to Identify Potent Co-Assembled Nanodrugs for Cancer Treatment.

Co-assembled nanodrugs provide significant advantages in cancer treatment and drug delivery, yet effective screening methods to identify molecular combinations for co-assembly are lacking. This study presents a screening strategy integrating ligand-based virtual screening (LBVS) and density functional theory (DFT) calculations to explore new molecular combinations with co-assembly capabilities. The accuracy of this screening was validated by synthesizing various co-assembled nanodrugs under mild conditions.

Polysaccharides from Astragalus membranaceus Bunge alleviate LPS-induced neuroinflammation in mice by modulating microbe-metabolite-brain axis and MAPK/NF-κB signaling pathway.

Neuroinflammation can lead to various neurodegenerative disorders, resulting in irreversible neurological dysfunction. Astragalus membranaceus Bunge polysaccharides (APS) present great potential in alleviating neuroinflammation; however, the specific mechanism underlying its neuroprotective effect remains unclear, leading to uncertain prospects for pharmaceutical applications. This study aims to elucidate the mechanism underlying APS-mediated inhibition of neuroinflammation in mice induced by lipopolysaccharide (LPS) through regulation of metabolic function, intestinal flora composition, and cell signaling transduction.

The host phylogeny and climate determine the gut bacteria of global insects.

Insects play an indispensable role in ecosystems; however, in recent years, the rapid decline in global insect diversity and abundance has posed a significant threat to our survival environment. Insect gut microbes play a crucial role in the survival of insects. Understanding their global traits will be advantageous for insect protection, thus safeguarding our environment.

Combined effects of microplastics and flupyradifurone on gut microbiota and oxidative status of honeybees (Apis mellifera L.).

The increasing accumulation of polystyrene microplastics (PS-MPs) and the widespread use of flupyradifurone (FPF) affect honeybee health adversely. However, the combined impact of PS-MPs and FPF toxicity on honeybees remains unknown. In this study, honeybee (Apis mellifera L.

Differentiation and characterization of volatile compounds in five common milk powders using HS-GC-IMS, HS-SPME-GC-MS, and multivariate statistical approaches.

Aroma is a key factor in milk powder quality evaluation and consumer choice. However, research has mostly focused on processing effects, with little on flavor differences among milk powders. This study analysed and identified the flavor characteristics of five common types of milk powders in China, including yak milk powder, donkey milk powder, camel milk powder, goat milk powder, and cow milk powder, using Headspace-Gas Chromatography-Ion Mobility Spectrometry (HS-GC-IMS), Headspace Solid-Phase Microextraction-Gas Chromatography-Mass Spectrometry (HS-SPME-GC-MS), and multivariate statistical analysis.

Mannitol: A novel chemical marker in stingless bee honey.

Stingless bee honey (SBH) has garnered attention as a natural sweetener and functional food, recognized for its exceptional biological activity and distinctive flavour. Compared to honey bee honey, SBH has lower annual production but commands a higher market value, making it more susceptible to adulteration. During an analysis of trehalulose in SBH, we unexpectedly identified the naturally occurring sugar alcohol, mannitol.

The Role of Pathogens in Bumblebee Decline: A Review.

Bumblebees, the most important wild pollinators in both agricultural and natural ecosystems, are declining worldwide. The global decline of bumblebees may threaten biodiversity, pollination services, and, ultimately, agricultural productivity. Several factors, including pesticide usage, climate change, habitat loss, and species invasion, have been documented in the decline of bumblebee species, but recent studies have revealed the dominating role of pathogens and parasites over any of these causes.

Phenolic Acid and Flavonoid Content Analysis with Antioxidant Activity Assessment in Chinese Honey.

The nutritional value of honey is closely related to its components, which are highly influenced by the botanic origin. ( (Franch.) var.

Investigations on Beekeeping and Breeding of in China.

The worldwide value of the honey bee as an agricultural animal is increasingly being recognized. Not only does the honey bee directly produce useful agricultural products, but also large portions of crops are dependent on the pollination activities of honey bees. (), the native honey bee of China, is widely distributed in the country.

Honey Robbing: Causes, Impacts and Preventive Measures.

Honey robbing, which typically occurs during times of food scarcity, is a perilous foraging strategy for bee colonies and presents a formidable challenge in the realm of beekeeping. This article provides a comprehensive and multifaceted exploration of honey robbing, including the morphology, behavioral traits, timing, and scope of this phenomenon. This exploration elucidates the specific manifestations of honey robbing, offering readers a deeper understanding of its various facets.

Marine natural products as a source of novel anticancer drugs: an updated review (2019-2023).

Marine natural products have long been recognized as a vast and diverse source of bioactive compounds with potential therapeutic applications, particularly in oncology. This review provides an updated overview of the significant advances made in the discovery and development of marine-derived anticancer drugs between 2019 and 2023. With a focus on recent research findings, the review explores the rich biodiversity of marine organisms, including sponges, corals, algae, and microorganisms, which have yielded numerous compounds exhibiting promising anticancer properties.

Discovery of Novel Neonicotinoids with Conjugated Diene Enabled by Cascade Allylation/Isomerization Synthetic Methodology.

As one of the most significant insecticides, neonicotinoids have played a pivotal role in crop protection and public sanitation. However, the high resistance and bee toxicity of neonicotinoid insecticides have attracted considerable attention. Herein, a series of neonicotinoid compounds with conjugated diene moieties were synthesized through the cascade allylation/isomerization reaction.

Glyphosate and spinetoram alter viral communities with different effects on antibiotic resistance genes in the bumblebee gut.

Limited research investigating the impact of pesticides on antibiotic resistance genes (ARGs) and viral community in the gut of wild animals. In this study, we employed metagenomic to investigate the effects of glyphosate and spinetoram on the gut viral communities, ARGs, and their interactions in a key wild pollinator, bumblebees. The results showed that both 2.

Analysis of Flavor Differences in Yak Milk Powder at Different Milk Production Stages by Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry.

The aroma of yak milk powder is a crucial sensory indicator for evaluating its quality and flavor. Yak milk powders collected from different lactation periods exhibit distinct flavors, but no studies have thoroughly investigated the aroma characteristics and variation patterns of yak milk powders across these periods. This study identified and analyzed the volatile compounds in freeze-dried colostrum powder (YCSP), freeze-dried mature milk powder (YMMP), and freeze-dried ending milk powder (YEMP) using headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS) and multivariate statistical analysis.

Complete Mitochondrial Genome of (Hymenoptera: Apidae) from Two Geographical Regions: Insights into Structure and Genetic Differentiation.

The honeybee plays a crucial role as a pollinator, contributing significantly to biodiversity and supporting ecological processes [...

Rosemarinic Acid-Induced Destabilization of Aβ Peptides: Insights from Molecular Dynamics Simulations.

Alzheimer's disease (AD) is a neurodegenerative disorder marked by the progressive accumulation of amyloid-β (Aβ) plaques and tau protein tangles in the brain. These pathological aggregates interfere with neuronal function, leading to the disruption of cognitive processes, particularly memory. The deposition of Aβ forms senile plaques, while tau protein, in its hyperphosphorylated state, forms neurofibrillary tangles, both of which contribute to the underlying neurodegeneration observed in AD.

Dietary Methionine Restriction Alleviates Cognitive Impairment in Alzheimer's Disease Mice via Sex-Dependent Modulation on Gut Microbiota and Tryptophan Metabolism: A Multiomics Analysis.

Plant-based foods with low methionine contents have gained increasing interest for their potential health benefits, including neuroprotective effects. Methionine restriction (MR) linked to a plant-based diet has been shown to mitigate neurodegenerative diseases such as Alzheimer's disease (AD) through mechanisms that involve the gut microbiota. In this study, a 16-week MR diet (0.

Insights into adult worker foraging dynamics within a Bombus terrestris (Hymenoptera: Apidae) colony.

Bombus terrestris, an important eusocial insect, plays a vital role in providing pollination services for both wild plants and greenhouse crops. For the development of the colonies, the workers must leave the hives to collect nectar and pollen. However, limited findings about the foraging behavior of B.

Assessing the distribution and human health risks of cationic surface-active agents in honey from China.

Cationic surface-active agents (CSAAs) can persist in ambient water, be ingested by bees, and contaminate honey. Residues of CSAAs in honey remains unknown. This study measured the residual levels of five CSAAs in 271 honey samples from China using ultrahigh-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry.

Mitochondrial mechanism of florfenicol-induced nonalcoholic fatty liver disease in zebrafish using multi-omics technology.

Florfenicol (FF), a third-generation chloramphenicol antibiotic widely used in food-producing animals, has become a "pseudopersistent" environmental contaminant, raising concerns about its potential ecological and human health impacts. However, its bioaccumulation behavior and hepatotoxic mechanisms remain poorly understood. This study aims to address these gaps with a 28-day exposure experiment in adult zebrafish at 0.

Novel neonicotinoid insecticide cycloxaprid exhibits sublethal toxicity to honeybee (Apis mellifera L.) workers by disturbing olfactory sensitivity and energy metabolism.

The risk of neonicotinoid insecticides to honeybees is a global issue. Cycloxaprid (CYC) is a novel neonicotinoid insecticide with outstanding activities, good safety profiles, and no cross-resistance with other neonicotinoids. Information on the environmental risks of CYC is limited, especially its effects on honeybees.