PRRSV evades innate immune cGAS-STING antiviral function via its Nsp5 to deter STING translocation and activation.

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is an important pathogen that seriously endangers pig breeding, causing significant economic losses to the global swine industry. Our previous study found that the DNA sensing innate cGAS-STING signaling pathway plays an important role in inducing interferon (IFN) upon PRRSV infection and inhibition of PRRSV replication. However, the mechanism underlying immune evasion by PRRSV remains unclear.

Characterization of Ginger Polysaccharide and Amelioration of High-Fat-Induced Obesity by Regulating the Energy Intake and Expenditure.

Ginger polysaccharide (GP) was obtained with a yield of 5.18%, and its main fraction GP-F1 (Mw = 109.24 kDa) was purified for structural characterization.

Exercise improves hypobaric hypoxia-induced skeletal muscle dysfunction via Sirt1-Mediated myotube and mitochondrial remodeling.

Introduction: Skeletal muscle function is profoundly challenged under high-altitude environments, where hypobaric hypoxia disrupts structural integrity and impairs physiological function. However, few animal studies have examined the impact of hypobaric hypoxia on skeletal muscle and molecular basis. While exercise training holds promise for alleviating hypoxia-induced muscle dysfunction, the understanding of its protective mechanisms remains limited.

Involvement of endocannabinoid receptor 1 in oxidative and inflammatory responses underlying anxiety-like behavior in SPS&S-exposed mice.

Objective: This study investigated the role of endocannabinoid (eCB) signaling in anxiety-like behaviors associated with a PTSD mouse model (SPS&S), focusing on oxidative stress and inflammation.

Methods: We examined expression of eCB-related proteins (Cannabinoid receptor type 1, CB1 Receptor; NAPE-specific phospholipase D, NAPE-PLD; Fatty acid amide hydrolase, FAAH; and Monoacylglycerol lipase, MAGL) and inflammatory markers (IL-1β, caspase-1) in the mPFC, hippocampus, and amygdala of SPS&S-exposed mice. Plasma oxidative stress markers (MDA, SOD, ROS) and cytokines (IL-1β, IL-6, TNF-α, COX-2) were measured by ELISA.

Inhibition of Setd7 protects against cardiomyocyte hypertrophy via inhibiting lipid oxidation.

Myocardial hypertrophy is one of the most prominent features of heart failure. SET domain-containing protein 7 (Setd7), a catalytic enzyme responsible for histone H3K4 methylation, has been implicated in various cardiac diseases. In this study we investigated whether Setd7 contributed to the development of cardiac hypertrophy.

Analysis of Protein Degradation and Umami Peptide Release Patterns in Stewed Chicken Based on Proteomics Combined with Peptidomics Approach.

Proteomics combined with peptidomics approaches were used to analyze the protein degradation and the release pattern of umami peptides in stewed chicken. The results showed that a total of 422 proteins were identified, of which 273 proteins consistently existed in samples stewed for 0-5 h. Myosin heavy chain exhibited the highest abundance (26.

Decoding the Molecular Mechanisms of Menthol Isomer Perception Based on Computational Simulations.

The flavor characteristics, perception, and molecular mechanisms of eight menthol isomers were investigated by sensory analysis combined with computational simulations. The sensory analysis results show significant differences in the odor profiles of the different menthol isomers. Among them, L-menthol shows a pleasant, sweet, and mint-like odor with a distinct freshness and no off-flavors, whereas the remaining seven isomers were interspersed with negative odors (musty, herbal, or earthy aromas).

Characterization of the aroma variances of duck broth from different part by HS-SPME-GC-MS, HS-GC-IMS and sensory evaluation.

China is the world's largest producer of meat ducks. Decoding the aroma composition of duck broth (DB) produced from different parts can help improve the sensory quality and diversified development of duck meat products. The aroma compounds of DB stewed from seven parts were characterized by solid-phase microextraction-gas chromatography-mass spectrometry, headspace-gas chromatography-ion mobility spectrometry, sensory evaluation, odor activity value (OAV) analysis, and partial least squares regression (PLSR) analysis.

Identification of saltiness-enhancing compounds in Zanthoxylum bungeanum essential oil chicken soup and molecular docking with transmembrane channel-like protein 4 (TMC4).

This study examines how adding Zanthoxylum bungeanum essential oil affects the sensory quality of chicken soup, focusing on identifying the mechanisms of saltiness-enhancing compounds. Sensory evaluation demonstrated that the essential oil significantly increased the soup's saltiness, umami and overall acceptance. Metabolomics and chemometrics identified unique (17 volatile and 27 non-volatile) and differential (11 volatile and 16 non-volatile) compounds with high expression levels in Z.

Huaganjian decoction ameliorates metabolic dysfunction-associated steatotic liver disease through liver-gut crosstalk.

Ethnopharmacological Relevance: Huaganjian decoction (HGJD), a traditional Chinese medicine formula, has been widely acknowledged for its remarkable effects in soothing the liver, relieving depression, and alleviating hepatic inflammation. Clinically, it has been extensively applied in the treatment of metabolic dysfunction-associated steatotic liver disease (MASLD). Nevertheless, the underlying mechanisms of its therapeutic actions remain incompletely understood, thus necessitating further in-depth investigations.

: Epidemiology and resistance evolution of an emerging zoonotic bacteria.

The health of humans and the growth of the agriculture industry are seriously threatened by the emerging zoonotic pathogen (). In the last decades, evolutionary studies have significantly enhanced our understanding of . Currently, is widespread across numerous countries, in which it displays diverse serotypes and complex population structures.

A comparative study on infant growth between assisted reproductive technology pregnancy and natural pregnancy.

Introduction: Globally, the number of assisted reproductive technology (ART) infants has surpassed 10 million, with its utilization steadily increasing. Numerous studies have shown that ART pregnancies are more prone to adverse pregnancy outcomes. ART infants are at a higher risk for adverse birth outcomes.

Inflammatory Bowel Disease Induces Colorectal Cancer: Risk Factors, Triggering Mechanisms, and Treatment With Phyto-Derivatives.

Colorectal cancer (CRC), one of the most prevalent malignant neoplasms, ranks as a leading cause of cancer-related mortality on a global scale. A particularly aggressive variant of CRC is colitis-associated colon cancer (CAC). Patients with chronic inflammatory bowel disease (IBD) are at increased risk for CAC.

Hyperoside inhibits PRRSV proliferation via the TLR4/NF-κB and p62-Nrf2-Keap1 signaling pathways, mediating inflammation and autophagy.

Porcine reproductive and respiratory syndrome virus (PRRSV) causes abortion and respiratory disease in swine, hindering the development of the pig farming industry worldwide. However, at present, there are no effective vaccines or drugs for PRRSV control. In this study, we evaluated the inhibitory effect of hyperoside on PRRSV replication and and explored the underlying mechanisms.

Loading Pt clusters is more conducive to photocatalytic hydrogen evolution compared to single atoms and nanoparticles.

Pt/TiO photocatalysts were synthesized using incipient wetness impregnation followed by oxidative and/or reductive thermal treatments. The loading dimensions of Pt elements on the TiO surface (Pt, Pt, and Pt) were controlled by varying the impregnated solution. Experimental results demonstrate that Pt with an intermediate size exhibits the highest photolysis rate under 300 W xenon lamp irradiation, achieving a hydrogen yield of 11.

Does atrial fibrillation increase the risk of fractures? A systematic review and meta-analysis.

Objectives: We have observed in clinical practice that patients with fractures often have concomitant atrial fibrillation. However, it remains unclear whether atrial fibrillation increases the risk of bone fracture. A meta-analysis was performed to investigate the association between atrial fibrillation and fracture.

Proteomics profiling reveals the potential targets of Apatinib inhibiting the proliferation of glioma U251 cell.

Glioblastoma multiforme (GBM) is an intracranial malignant tumor that seriously threatens human life. Recently, studies have reported that solid glioma can be effectively treated with Apatinib by inhibiting vascular endothelial growth factor receptor-2 (VEGFR-2) tyrosine kinase from affecting the formation of new blood vessels. However, whether Apatinib can inhibit the proliferation of glioma cells by affecting potential targets beyond angiogenesis in glioma remains unclear.

Development and temporal external validation of a novel nomogram for predicting one-year mortality in the older adult with hip fracture.

Objective: The aim of this study was to develop a novel nomogram for predicting one-year mortality in the older adult patients with hip fracture and to further evaluate its effectiveness.

Methods: This retrospective cohort research analyzed the clinical data of 1,263 older adult patients with hip fractures who underwent surgery at the First Affiliated Hospital of Air Force Military Medical University from January 2014 to December 2022. Patients receiving surgical treatment during January 2014 to December 2019 (864 cases) for the model development and further, data from the same centre with same inclusion criteria from January 2020 to December 2022 (399 cases) for the external validation of the model.

Cross-Cultural Reliability of Chinese and Dutch PAIC15 Pain Observations in Stroke and Dementia.

Purpose: Observational pain assessment tools provide important information for healthcare staff in patients who have difficulty expressing themselves. Nonverbal pain expressions are influenced by cultures and may affect the accurate assessment of pain in raters and patients from different cultural backgrounds. It is unknown if observational pain assessment tools that have been developed in Western cultures can be applied reliably in non-Western cultures and whether pain can be measured reliably across cultures.

Hypobaric hypoxia-driven energy metabolism disturbance facilitates vascular endothelial dysfunction.

Hypobaric hypoxia in plateau environments inevitably disrupts metabolic homeostasis and contributes to high-altitude diseases. Vascular endothelial cells play a crucial role in maintaining vascular homeostasis. However, it remains unclear whether hypoxia-mediated changes in energy metabolism compromise vascular system stability and function.

BBB proteomic analysis reveals that complex febrile seizures in infancy enhance susceptibility to epilepsy in adulthood through dysregulation of ECM-receptor interaction signaling pathway.

Background: Complex febrile seizures (CFS) have been associated with an increased risk of epilepsy in adulthood. However, the specific link between blood-brain barrier (BBB) and the predisposition to epilepsy in adults who experienced CFS during infancy remains unclear. The objective of this study was to investigate the alteration of BBB in adult mice who had experienced CFS during infancy, and to explore the mechanisms of increased susceptibility to epilepsy after CFS.

Rescue of naïve porcine circovirus type 3 and its pathogenesis in CD pigs.

Unlabelled: The pathogenesis of naïve porcine circovirus type 3 (PCV3) in pigs is not accurately elucidated due to virus isolation failure . In this study, naïve PCV3 was successfully rescued and used to infect cesarean-derived (CD) pigs. The PCV3 genome was synthesized, cloned, and inserted into the pBluescript SK vector.

Integrating Dense Genotyping with High-Throughput Phenotyping Empowers the Genetic Dissection of Berry Quality and Resilience Traits in Grapevine.

Investigating the genetic architecture of important agronomic traits in grapevine, like berry quality and resilience to abiotic stress, has been hampered by bottlenecks in genotyping and phenotyping. To address these limitations, this study aimed to develop innovative tools to unravel the complex polygenic genomic architecture of these traits. Specifically, a high-density 200K single nucleotide polymorphism array is developed and validated its effectiveness by genotyping 471 accessions from three F breeding populations.

Natural products targeting ferroptosis in depression: Research progress and therapeutic prospects.

Background: Depression is recognized as a chronic mental illness, also influenced by neurotransmitter homeostasis, with its incidence increasing annually worldwide. This condition inflicts significant physical and psychological harm, severely compromising human health. It exhibits a broad morbidity spectrum, and some current treatments and medications are hindered by short-term efficacy, strong side effects, and other limitations.

Synergistic effects of mannoprotein and ultrasound on the interfacial properties, flavor, and structure of yeast protein.

To expand the application of yeast protein (YP) in the food industry, the synergistic effects of mannoprotein (MP) and ultrasound (US) treatment were explored to improve its solubility, flavor, and structural properties. Results showed that YP and MP formed YP-MP conjugation in 1:3 (w:w), after reacting with citric acid and sodium bicarbonate for 4 h at room temperature and ultrasonic treatment at 1000 W for 30 min. The YP-MP-US sample achieved a maximum protein yield of 54.