Self-pumping Janus nanofiber membrane with pH monitoring capability, integrated with a drug-loaded fast-dissolving layer for enhanced chronic wound healing.

The emergence of antimicrobial resistance poses significant challenges in conventional antibiotic treatments for chronic wound infections, highlighting an urgent need for alternative therapeutic strategies. To address this issue, we developed a multifunctional electrospun nanofiber dressing co-loaded with anthocyanin (ATH) and asiaticoside (AS) that possesses antimicrobial activity. The tri-layer dressing contains three functional components: a hydrophilic polyacrylonitrile-anthocyanin (PAN-ATH) layer for pH monitoring, a hydrophobic polycaprolactone (PCL) layer for exudate management, and a water-soluble pullulan-Bletilla striata polysaccharide-asiaticoside (PUL-BSP-AS) layer.

m1A methylase TRMT6 promotes neuroblastoma development by demethylating SST mRNA in an m1A/YTHDF2-dependent manner.

Background: m1A, a prevalent RNA modification found in various RNA species, has recently been reported to modulate cancer progression. However, its effects on neuroblastoma remain uninvestigated.

Methods: The PCAT database was utilized to analyze the mRNA levels and survival probabilities of m1A regulator genes (TRMT6, TRMT61A, ALKBH1, and ALKBH3) in neuroblastoma patients.

Emerging role of the TCA cycle and its metabolites in lung disease.

The TCA cycle is a central pathway for oxidative phosphorylation in eukaryotic mitochondria, plays a role in energy production and biosynthesis, and can alter cellular function in a variety of ways. As our understanding of the TCA cycle has improved, there is increasing evidence that it is inextricably linked to lung disease. This article summarizes the relationship between the TCA cycle and different types of lung disease and focuses on the specific mechanisms by which the TCA cycle and its intermediate metabolites influence lung disease progression.

Enzymatic Synthesis and in vitro Digestion Characteristics of Human Milk Fat Substitutes 1,3-Dilinoleoyl-2-palmitoylglycerol.

1,3-dilinoleoyl-2-palmitoylglycerol (LPL) is an important structural lipid in breast milk fat, which plays an important role in the health of infants, and therefore the development of an efficient method for the preparation of such compounds is necessary. In the present study, LPL was efficiently catalytically synthesized by immobilized lipase ANL-MARE as a biocatalyst using tripalmitate and linoleic acid in a solvent-free system, and its digestive properties were investigated. The optimal process conditions for the enzymatic acidolysis of LPL were optimized by response surface test: the molar ratio of PPP:LA was 1:10, the enzyme addition was 13.

Maternal liver lipid dysregulation mediates fetal developmental impairments induced by gestational co-exposure to ozone and heat in C57BL/6J Mice.

Global warming has increased the frequency of simultaneous ozone and heat exposure, raising significant public health concerns. This study investigates the impacts of gestational exposure to ozone (1 ppm) and heat (34°C) under real-world conditions on fetal and placental development in C57BL/6 J mice, with a particular focus on the role of maternal hepatic lipid metabolism. Pregnant mice were exposed from gestational day (GD) 0 to GD17.

Establishment and application of a triplex RTqPCR assay system for the identification of forensic body fluids.

MicroRNAs (miRNAs) have emerged as valuable biomarkers for the identification of forensic body fluids due to their stability and tissue specificity. However, the limited quantity of body fluid at crime scenes often hampers the accuracy of miRNA-based detection methods. In this study, we developed a triplex reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay system that enables the simultaneous detection of three miRNAs, improving throughput and efficiency while overcoming challenges in forensic investigations.

Ppp2r1a haploinsufficiency increases excitatory synaptic transmission and decreases spatial learning by impairing endocannabinoid signaling.

Protein phosphatase 2A (PP2A) is a serine/threonine phosphatase in the brain. Mutations in PPP2R1A, encoding the scaffolding subunit, are linked to intellectual disability, although the underlying mechanisms remain unclear. This study examined mice with heterozygous deletion of Ppp2r1a in forebrain excitatory neurons (NEX-het-conditional knockout [NEX-het-cKO]).

Contrast-enhanced ultrasound in evaluating the curative effect of interventional therapy in patients with liver cancer.

Background: Contrast-enhanced ultrasonography (CEUS) is utilized to assess the therapeutic efficacy of interventional therapy in liver cancer patients, offering insights into tumor blood flow changes, angiogenesis, and tumor markers.

Aim: To evaluate the use of CEUS in examining the effectiveness of interventional therapy for liver cancer, we aim to investigate its diagnostic utility for tumor perfusion patterns, microvessel density, perfusion recovery, blood flow enhancement response, and alterations in tumor markers among patients receiving interventional therapy for liver cancer.

Methods: The study involved 124 patients who underwent interventional therapy for liver cancer at Guangzhou First People's Hospital from January 2022 to February 2024.

Serum IL-6, IL-10, TNF-a changes in postpartum perineal oedema rehabilitation.

Background: This study aims to evaluate the effectiveness of manual lymphatic drainage (MLD) combined with abdominal breathing in rehabilitating postpartum perineal oedema and Serum IL-6, IL-10, TNF-a.

Methods: A total of 172 primiparous women who delivered in our hospital between January 2022 and June 2023 were randomly assigned to either the observation group (n=86) or the control group (n=86). The control group received routine midwifery care, while the observation group received additional MLD and abdominal breathing training.

Effects of moderate intensity continuous training combined with emotional freedom techniques on fatigue in patients with chronic obstructive pulmonary disease: A four-arm parallel randomized controlled trial.

Fatigue is one of the most common and distressing symptoms in patients with chronic obstructive pulmonary disease (COPD). This study applied MICT, EFT and their combination (MICT and EFT) to COPD patients with fatigue through factorial design analysis method, aiming to explore non-drug management strategies that are more conducive to relieving fatigue in COPD patients. This study had a total of 112 participants.

Hidden blood loss of percutaneous vertebroplasty in the treatment of spinal metastases of breast cancer.

Objectives: The aim of this study was to evaluate hidden blood loss (HBL) and to identify its possible risk factors after percutaneous vertebroplasty (PVP) in patients with spinal metastases from breast cancer.

Patients And Methods: Between January 2020 and January 2024, a total of 54 female patients (mean age: 65.3±7.

Fate and removal of per- and polyfluoroalkyl substances throughout landfill leachate treatment processes: Uncovering precursor transformation and sludge-mediated reappearance.

Per- and polyfluoroalkyl substances (PFAS) in municipal solid waste (MSW) landfill leachate have emerged as a critical research focus because of their substantial environmental and health risks. However, the fate and transformation of PFAS across different treatment processes, particularly within specific techniques such as biotreatment, remains understudied in current research. This study systematically investigated the occurrence, fate, and removal of PFAS in a full-scale MSW landfill leachate treatment system in Shanghai, China.

Association between serum uric acid levels and white matter hyperintensities in patients with mild ischemic stroke.

Objective: This study aims to investigate the association between uric acid (SUA) level and white matter hyperintensities (WMH) in patients with mild ischemic stroke.

Methods And Results: This study retrospectively analyzed 290 patients with mild ischemic Stroke who were hospitalized at the Second Hospital of Hebei Medical University from March 2021 to January 2022. General clinical information and laboratory test results were collected.

Scaffold-leaping optimization of naphthoquinone derivatives as ferroptosis inducer against non-small lung cancer.

Ferroptosis, an iron-dependent programmed cell death pathway, has emerged as a promising therapeutic target for cancer. Herein, a series of naphthoquinone derivatives were designed via scaffold-leaping optimization from lead compound QD394, synthesized and assessed for their anti-proliferation activity against five cancer cell lines, and the structure-activity relationship (SAR) were described. Of these compounds, I-21 was identified as most active, demonstrating significant anticancer efficacy in vitro (IC = 0.

The involvement of m6A RNA methylation in the transgenerational inheritance of hepatotoxicity induced by paternal cadmium exposure.

Cadmium (Cd), a widespread environmental pollutant, has linked with various adverse health effects. However, the paternally inherited transgenerational effects of cadmium exposure on hepatotoxicity inoffspring and the underlying mechanism remained undefined. To address this issue, male mice (F0) were administrated with 100 mg/L cadmium chloride (CdCl) in drinking water for 3 months.

Lumbar and pelvic CT image segmentation based on cross-scale feature fusion and linear self-attention mechanism.

The lumbar spine and pelvis are critical stress-bearing structures of the human body, and their rapid and accurate segmentation plays a vital role in clinical diagnosis and intervention. However, conventional CT imaging poses significant challenges due to the low contrast of sacral and bilateral hip tissues and the complex and highly similar intervertebral space structures within the lumbar spine. To address these challenges, we propose a general-purpose segmentation network that integrates a cross-scale feature fusion strategy with a linear self-attention mechanism.

Prevalence and Genomic Characterization of Isolated from a Vast Amount of Aquatic Products in Huzhou, China.

is the leading bacterial cause of gastroenteritis associated with aquatic food consumption globally. This study aimed to determine the prevalence of in aquatic foods from Huzhou and to identify the serotypes, antimicrobial resistance, virulence factors, and genetic relatedness of the strains. A total of 306 isolates were detected from 1314 aquatic food samples from 2022 to 2024.

Changes in choriocapillaris blood flow density and associated factors affecting axial elongation in Chinese children and adolescents with mild to moderate myopia: a cross-sectional study.

Background: To investigate changes in choriocapillaris blood flow density (CFD) and identify factors influencing axial elongation in Chinese children and adolescents with mild to moderate myopia.

Methods: A hospital-based cross-sectional study was conducted. A total of 288 participants aged 8 to 15 years were recruited from Tianjin Eye Hospital, including 186 children and adolescents with myopia and 102 age-matched participants without refractive error who served as the control group.

Efficacy of traditional Chinese medicine prescription in treatment of COVID-19: A prospective cohort study.

Background: The purpose of this study was to estimate the efficacy of traditional Chinese medicine (TCM) prescription in the treatment of coronavirus disease 2019 (COVID-19).

Methods: A multicenter prospective cohort study at 7 hospitals was conducted. The COVID-19 inpatients were divided into 2 groups.

Efficient wheat variety identification using Raman hyperspectral imaging in combination with deep learning.

Wheat (Triticum aestivum L.) is recognized as a globally important staple crop, with its varietal differences influencing food processing, nutritional value, and agricultural productivity. Traditional identification methods are often considered inefficient and subjective, while existing spectral techniques are hindered by complex preprocessing procedures and limited model interpretability.

The value of ultrasonographic scoring method and nomogram in assessing cervical lymph node metastasis of papillary thyroid carcinoma.

Background: The preoperative cervical lymph node metastasis (CLNM) status in patients with papillary thyroid carcinoma (PTC) critically determines the type of lymph node dissection performed. Currently, ultrasonography is the primary method for initial CLNM screening in PTC patients. This study aims to analyze the efficacy of an ultrasonic-characteristics-based scoring system in diagnosing lymph node metastasis in PTC and construct a predictive nomogram.

Organizational innovation climate and nurses' innovation behavior in a specialized oncology hospital: the chain mediation of achievement motivation and creative self-efficacy.

Objective: This study explains the impact of organizational innovation climate on nurses' innovation behavior in a tertiary specialized oncology hospital, while examining the chain mediation roles of achievement motivation and creative self-efficacy.

Methods: In this online cross-sectional survey, 857 nurses from a tertiary specialized oncology hospital in Guangdong province, China were selected by convenience sampling method. SPSS Statistics v26.

Aberrant Notch-signaling promotes tumor angiogenesis in esophageal squamous-cell carcinoma.

Esophageal squamous-cell carcinoma (ESCC) is one of the most common gastrointestinal cancers in China, characterized by high malignancy and poor prognosis. Nowadays, the therapeutic options for this cancer are very limited. Notch-signaling is often overactivated in ESCC, but its role remains to be fully elucidated.

Tetrahydrocurcumin ameliorates metabolic disorders associated with obesity by regulating gut microbiota homeostasis.

Obesity, a global health crisis, demands novel therapeutic strategies. Tetrahydrocurcumin (THC), a natural compound, shows promise in mitigating obesity by regulating gut microbiota and lipid metabolism. Using in vitro (Hepa1-6 cells) and in vivo (C.