Protocol for evaluating protein intake-induced feeding inhibition in Drosophila using a pre-feeding approach.

Consumption of dietary protein has been shown to elicit the strongest satiety among macronutrients. Protein intake-induced feeding inhibition (PIFI) plays a critical role in the maintenance of nutrition homeostasis. Here, we present an optogenetic pre-feeding protocol for studying the neural mechanisms underlying PIFI in Drosophila.

Periplocin potentiates ferroptotic cell death in non-small cell lung cancer by inducing the degradation of Nrf2.

Background: Non-small cell lung cancer represents the main histological subtype of lung cancer. Periplocin is a major cardiac glycoside found in the traditional Chinese medicine Cortex periplocae administered in cardiovascular and autoimmune diseases. Inspired by recent findings reporting the anticancer activities of periplocin, this study investigates its potential effects against lung cancer.

Scaffold-leaping optimization of naphthoquinone derivatives as ferroptosis inducer against non-small lung cancer.

Ferroptosis, an iron-dependent programmed cell death pathway, has emerged as a promising therapeutic target for cancer. Herein, a series of naphthoquinone derivatives were designed via scaffold-leaping optimization from lead compound QD394, synthesized and assessed for their anti-proliferation activity against five cancer cell lines, and the structure-activity relationship (SAR) were described. Of these compounds, I-21 was identified as most active, demonstrating significant anticancer efficacy in vitro (IC = 0.

Subthreshold anxiety in Chinese college students: prevalence, gender differences, and correlates.

The aim of the study is to analyze the prevalence of subthreshold anxiety(SA) among Chinese college students and the correlates of SA. The data was from the 2022 Psychology and Behavior Investigation of Chinese Residents. Stepwise logistic regression was employed to discern the correlates of SA.

N6-methyladenosine methylation: a novel key to unlocking mental disorders.

More than 100 types of RNA modifications have been identified in mammalian cells, among which N6-methyladenosine (m6A) is the most prevalent. This reversible and dynamic modification involves methyltransferases, demethylases, and reader proteins. Aberrant expression of m6A-related regulatory proteins in the nervous system significantly impacts neuronal physiology, contributing to mental disorders such as depression, autism spectrum disorder, and schizophrenia.

The sleep quality and its association with disease activity in patients with inflammatory bowel disease: a meta-analysis.

Background: Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is linked to poor sleep quality, though evidence remains inconsistent. This meta-analysis aimed to evaluate sleep quality in IBD patients and its association with disease activity.

Methods: We systematically searched databases up to February 2025 for studies reporting sleep quality in IBD.

Detection and Characterization of (Hepatitis E Virus) in Dairy Products from Hebei Province, China.

(hepatitis E virus), a zoonotic pathogen transmitted primarily via the fecal-oral route, has undergone shifting transmission dynamics in China, with foodborne and zoonotic routes becoming increasingly significant. To assess the potential risk of HEV transmission through dairy products, this study investigated HEV RNA presence in raw milk from cows, sheep, and goats in Hebei Province, China. From March 2024 to April 2025, we collected 102 cow milk, 18 sheep milk, and 59 goat milk samples, analyzing them using RT-qPCR, with positive samples confirmed by RT-Nested PCR and partial ORF2 sequencing.

Skeletal ossification of Middle Triassic pachypleurosaur (Reptilia: Sauropterygia) revealed by zinc distribution.

The elemental composition of seven specimens of , a small pachypleurosaur from the Middle Triassic of China, was analyzed Micro X-ray fluorescence (Micro-XRF). The results indicate that the distribution of zinc (Zn) is notably correlated with the bones of the skeletons and preferentially enriched in certain areas, unlike other bone-enriched elements such as calcium (Ca), phosphorus (P), and strontium (Sr). Based on the distribution of Zn and its chemical properties, Zn is interpreted as a potential indicator of active ossification in A comparative analysis of juvenile and subadult specimens reveals distinct patterns of Zn enrichment, reflecting differential bone development across ontogenetic stages.

Multi-omics approaches to explore the therapeutic mechanism for ginsenoside Rg1 against MASLD.

Ginsenoside Rg1 (G-Rg1), a traditional Chinese medicine, alleviates metabolic dysfunction-associated steatotic liver disease (MASLD). However, the mechanism by which G-Rg1 improves metabolic disorders in MASLD by regulating the gut microbiota remains ambiguous‌. We constructed a diet-induced murine MASLD model and employed 16S rRNA sequencing and non-targeted metabolomic analysis to ‌investigate the mechanism of G-Rg1 in treating MASLD, focusing on its regulatory effect on the gut microbiota.

Genome-Wide Identification, Phylogeny, and Abiotic Stress Response Analysis of Family Genes in the Alpine Medicinal Herb .

Hyperosmolality-gated calcium-permeable cation channel protein denoted as OSCA, which are mechanosensitive pore-forming ion channels, play a pivotal role in plants' responses to abiotic stressors. , an endemic perennial plant species distributed in the Qinghai-Tibetan Plateau and its adjacent high-altitude regions, is likely to have undergone adaptive evolution in response to extreme abiotic stress conditions. The current study was conducted to characterize the genome-wide characteristics and phylogenetic evolution of the gene family in and identify its response patterns to drought and high-temperature stresses.

Effects of cellulase or Lactobacillus plantarum on ensiling performance and bacterial community of sorghum straw.

This study aimed to evaluate the effects of cellulase or Lactobacillus plantarum (L. plantarum) on the fermentation characteristics and microbial community structure of the sorghum straw silage. Sorghum straw was treated with the following four experimental conditions: distilled water (control, CK), cellulase (CEL), Lactobacillus plantarum (LP), and a combined treatment of Lactobacillus plantarum with cellulase (LPCEL).

Mamba-based deformable medical image registration with an annotated brain MR-CT dataset.

Deformable registration is essential in medical image analysis, especially for handling various multi- and mono-modal registration tasks in neuroimaging. Existing studies lack exploration of brain MR-CT registration, and face challenges in both accuracy and efficiency improvements of learning-based methods. To enlarge the practice of multi-modal registration in brain, we present SR-Reg, a new benchmark dataset comprising 180 volumetric paired MR-CT images and annotated anatomical regions.

Dynamic changes of hippocampal dendritic spines in Alzheimer's disease mice among the different stages.

Alzheimer's disease (AD) is characterized by the accumulation of amyloid-β (Aβ) peptides and a progressive decline in cognitive function. Hippocampus as a crucial brain area for learning and memory, is also adversely affected by AD's pathology. The accumulation of Aβ is often associated with the loss of dendritic spines of the hippocampus.

Acupuncture alleviates CSDS-induced depressive-like behaviors by modulating synaptic plasticity in vCA1.

Acupuncture (Acu) has been clinically validated as an effective treatment for depression. However, the underlying mechanism of Acu treatment's antidepressant effect remains unclear. We investigate the antidepressant effects of Acu treatment at the LR3 point in mice subjected to chronic social defeat stress (CSDS).

A simple and effective method to enhance the level of gamma-aminobutyric acid in Chinese yam tubers while preserving its original appearance.

Hot-air drying is an effective method to enhance the levels of gamma-aminobutyric acid (GABA) in edible tubers/tuberous roots. However, consumers prefer fresh food to processed food. Therefore, this study aims to develop an effective method to increase the GABA levels in the tubers of Chinese yam (CY tubers) and the tubers/tuberous roots of other plants while preserving its original appearance.

Cathodic Hydroxide Ions Induce Tetrose Formation during Glycolaldehyde Electroreduction to Alcohols: A Potential CO-to-Carbohydrate Pathway.

The electrochemical synthesis of organic compounds from CO can potentially alleviate climate change by hampering the atmospheric accumulation of greenhouse gases. The production of carbohydrates from CO reduction will have promising applications for the manufacturing of valuable, multi-carbon compounds that are traditionally produced from the petrochemical or agricultural industries. In this work, we analyzed the copper-catalyzed electrochemical reduction of glycolaldehyde, a commonly observed trace CORR product that has been previously proposed as an intermediate for alcohol formation.

Targets selection and field evaluation of an RNA biopesticide to control Phyllotreta striolata.

Phyllotreta striolata is a major insect pest that threatens vegetable crops worldwide. Recently, its increasing resistance to chemical pesticides underscores the urgent need for novel control strategies. This study explores the potential of RNA biopesticides for managing P.

Uncovering the Prevalence and Genetic Characterization of Rabbit-Derived Paslahepevirus Balayani (Hepatitis E Virus) in Hebei Province, China.

Paslahepevirus balayani (hepatitis E virus) is a zoonotic pathogen, with rabbit Paslahepevirus balayani (HEV-3ra) being widely distributed among global rabbit populations. Notably, in China, rabbits constitute a significant HEV host, second only to swine. Emerging evidence suggests that HEV-3ra possesses the capability to cross species barriers and infect humans.

Unsupervised Domain Adaptation for Cross-Modality Cerebrovascular Segmentation.

Cerebrovascular segmentation from time-of-flight magnetic resonance angiography (TOF-MRA) and computed tomography angiography (CTA) is essential in providing supportive information for diagnosing and treatment planning of multiple intracranial vascular diseases. Different imaging modalities utilize distinct principles to visualize the cerebral vasculature, which leads to the limitations of expensive annotations and performance degradation while training and deploying deep learning models. In this paper, we propose an unsupervised domain adaptation framework CereTS to perform translation and segmentation of cross-modality unpaired cerebral angiography.

Enhancer RNA Transcriptome-Wide Association Study Reveals a Distinctive Class of Pan-Cancer Susceptibility eRNAs.

Many cancer risk variants are located within enhancer regions and lack sufficient molecular interpretation. Here, we constructed the first comprehensive atlas of enhancer RNA (eRNA)-mediated genetic effects from 28 033 RNA sequencing samples across 11 606 individuals, identifying 21 073 eRNA quantitative trait loci (eRNA-QTLs) significantly associated with eRNA expression. Mechanistically, eRNA-QTLs frequently altered binding motifs of transcription factors.

The Efficacy and Safety of Adjustable Intragastric Balloon for Weight Loss: A Systematic Review and Meta-Analysis.

Introduction: Adjustable intragastric balloon (aIGB), known as a novel minimally invasive therapy for obesity, provides a sustained alternative for weight loss. Weight loss is achieved by implanting a balloon into the patient's stomach, with the volume of the balloon being adjustable through the injection or extraction of physiological saline. Its key distinction from other intragastric balloons lies in post-implantation volume control.

Design, synthesis and biological evaluation of benzo[b]thiophene analogues as novel ferroptosis inhibitor that inhibit fibrosarcoma cell proliferation.

While apoptosis activation has traditionally been considered as an anti-cancer mechanism, current research points to ferroptosis stimulation as a potentially effective cancer therapy. Glutathione peroxidase 4 (GPX4), an essential antioxidant enzyme, serves as a negative regulator of ferroptosis, and its targeted inhibition or degradation can efficiently induce this process. In this study, a potent ferroptosis inducer III-4 that bearing a benzo[b]thiophene moiety was developed by employing a sequential structure optimization process based on RSL-3 to inhibit cancer cells proliferation.

Exosomal mir-126-3p derived from endothelial cells induces ion channel dysfunction by targeting RGS3 signaling in cardiomyocytes: a novel mechanism in Takotsubo cardiomyopathy.

Background: Takotsubo cardiomyopathy (TTC) is marked by an acute, transient, and reversible left ventricular systolic dysfunction triggered by stress, with endothelial dysfunction being one of its pathophysiological mechanisms. However, the precise molecular mechanism underlying the interaction between endothelial cells and cardiomyocytes during TTC remains unclear. This study reveals that exosomal miRNAs derived from endothelial cells exposed to catecholamine contribute to ion channel dysfunction in the setting of TTC.

Dynamic relationships between environment-related technologies, agricultural value added, transport infrastructure and environmental emissions in the five most populous countries.

Densely populated countries have higher demand for agricultural products and greater use of fuel and vehicles, resulting in higher environmental emissions, so this study considers transportation infrastructure and the agricultural sector as the two major drivers of environmental degradation in the top five densely populated countries. In a baseline regression, this study first unveils the impact of investments in transportation infrastructure, agricultural value addition, and environment-related technologies on per capita CO emissions using CS-ARDL approach from 1990 to 2021 in the five most populous countries. Besides, this study also reveals the typical moderating effect of environment-related technologies in the link between transport infrastructure, agricultural value added and environmental hazards.