CD93: A Promising NETs-Related Biomarker for Diagnosis and Therapy in Actinic Keratosis.

Background: Actinic keratosis (AK), a UV-induced precancerous skin condition potentially progressing to cutaneous squamous cell carcinoma (cSCC) with undefined mechanisms, was analyzed for neutrophil extracellular traps (NETs)-related biomarkers to identify key clinical targets.

Methods: Transcriptomic profiles of AK retrieved from the GEO database were analyzed using the "limma" package to screen differentially expressed genes (DEGs), which were intersected with a curated NETs-related gene set to extract differentially expressed NETs-related genes (DE-NRGs). Functional enrichment analyses via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations identified enriched biological processes and pathways.

A Highly Efficient 5S rRNA-CRISPR/Cas9 Genome Editing Toolkit in .

is an important industrial producer of cephalosporin C (CPC), and efficient genome editing tools are critical for its exploitation and metabolic engineering. Currently, CRISPR/Cas9 systems for employ heterologous promoters, including P or AfU6p, to drive sgRNA expression. These systems often required additional sgRNA processing elements such as ribozymes or tRNAs, which increased cloning complexity and experimental workload.

School and Healthcare Collaboration in Implementing and Sustaining School-Based Health Centers in Rural Communities.

Background: This study examines how school-based health centers (SBHCs) are implemented and sustained through schools and a healthcare system in a four-county rural region in New York State.

Methods: Twelve semi-structured interviews were conducted with school superintendents and employees of the healthcare system, including SBHC medical providers (physician assistant, mental health therapist, nurse), SBHC manager, care coordinator, Chief of Pediatrics, and executive leader between January and April 2024. Interviews were thematically coded using collective impact and collective action frameworks.

Characteristics of personal exposure to metals in PM and their implications for epidemiological studies: New insights from a panel study of the elderly in Beijing.

Air pollution levels monitored at fixed sites, known as ambient concentrations, are often used as surrogate indicators of individual exposure levels. However, this could lead to exposure misclassification, a major challenge in environmental epidemiology research. Discrepancies between ambient concentrations and personal exposure characteristics, their underlying causes, and their potential impacts on health effect assessments remain unclear, particularly for the metals in fine particulate matter (PM).

HLA-DOB: A Key "Coordinator" Between Cutaneous Melanoma and Psoriasis.

Psoriasis is a chronic inflammatory skin disease associated with immune dysfunction, and its relationship to cutaneous melanoma is unclear. This study used Mendelian randomization (MR) to explore the causal link between the two and identify risk genes. SNPs from a psoriasis GWAS (5,072 cases, 478,102 controls) were used as instrumental variables, and melanoma GWAS data (3,751 cases, 372,016 controls) served as the outcome.

Application and research progress of tranexamic acid in the perioperative period of posterior lumbar interbody fusion.

Posterior lumbar interbody fusion requires stripping the multifidus muscle, destroying a large amount of cancellous bone and damaging the posterior spinal venous plexus. Typically, surgical trauma is extensive, the surgical duration is long, and the degree of bleeding is substantial. Excessive blood loss can compromise a patient's hemodynamic stability, elevate surgical risks, and cause damage to vital organs, potentially becoming life-threatening in severe cases.

Expert consensus on the diagnosis and treatment of cemental tear.

Cemental tear is a rare and indetectable condition unless obvious clinical signs present with the involvement of surrounding periodontal and periapical tissues. Due to its clinical manifestations similar to common dental issues, such as vertical root fracture, primary endodontic diseases, and periodontal diseases, as well as the low awareness of cemental tear for clinicians, misdiagnosis often occurs. The critical principle for cemental tear treatment is to remove torn fragments, and overlooking fragments leads to futile therapy, which could deteriorate the conditions of the affected teeth.

Study of the reversal of metallo-β-lactamase-mediated resistance in Gram-negative bacteria by EDTMP.

Metallo-β-lactamases (MBLs) are major causes of carbapenem resistance in Gram-negative bacteria. In this study, a simple and practical method was developed to synthesize the fluorescent probe CE-HF, which was proven effective for detecting the activity of the MBL NDM-1. Using this probe, ethylenediamine tetra(methylene phosphonic acid) (EDTMP) was identified as a potent MBL inhibitor with strong activity (IC₅₀ = 0.

Poly (ADP-Ribose) Glycohydrolase-Dependent dePARylation of PCNA Is Essential for DNA Replication.

Poly (ADP-ribosyl)ation (PARylation) plays a crucial role in DNA replication, particularly during S phase, where it is detected at replication sites on the lagging strand to facilitate Okazaki fragment processing. However, the role of dePARylation in DNA replication remains elusive. In this study, we demonstrate that poly (ADP-ribose) glycohydrolase (PARG) is actively involved in degrading poly (ADP-ribose) at DNA replication sites during S phase.

KLF5 Regulation of Exosome-Derived miR-152-3p From Bone Marrow Stem Cells Improves Ventricular Arrhythmia After Myocardial Infarction.

Cardiac fibroblasts (CFs) are activated into cardiac myofibroblasts (CMFs) in myocardial infarction (MI) and promote fibrosis, playing a crucial role in deteriorating cardiac function and inducing fatal arrhythmias. Transplantation of bone marrow mesenchymal stem cells (BMSCs) has emerged as a promising therapeutic approach for ischemic heart diseases, including MI. Recent studies have indicated that BMSCs can modulate the survival, differentiation, and antifibrotic activity of CFs.

Discovery of a dual-action compound for metallo-β-lactamase inhibition and biofilm clearance to reverse CRE resistance.

The emergence of carbapenem-resistant Enterobacteriaceae (CRE) poses a severe threat to global public health due to its multidrug resistance and limited treatment options. In this study, QA, a zinc ion chelator with potent inhibitory activity against metallo-β-lactamases (MBLs), was identified through high-throughput screening and biochemical analysis. QA effectively inhibits MBLs by chelating zinc ions and demonstrates potent biofilm clearance capabilities, ultimately reversing carbapenem-resistant Enterobacteriaceae (CRE) resistance.

Maternal factors associated with plasma concentrations of endocrine-disrupting persistent organic pollutants including PFAS among Hispanic women from pregnancy to the post-delivery period.

Objective: Upon exposure, persistent organic pollutants (POPs) accumulate in the body. One-time point measurement of POPs plasma concentrations can reflect body burden. This study aimed to assess whether maternal factors including age, breastfeeding, and weight, influence changes in plasma concentrations of POPs in women during critical periods of physical changes from pregnancy to 15-60 months after delivery.

The change of bacterial community structure helped alleviate the pressure of drought stress.

Introduction: Drought stress poses a significant threat to plant growth and development, thereby adversely impacting agricultural productivity and ecosystem stability. In recent years, increasing attention has been given to plant-microorganism interactions as a promising strategy to enhance plant resistance to abiotic stresses.

Methods: In this study, we evaluated the effects of microbial inoculation on the growth, photosynthetic performance, nutrient uptake, and root morphology of Salvia miltiorrhiza under drought stress.

Characterization of grating monochromator performance based on start-to-end FEL pulse propagation method.

Grating monochromators are crucial optical elements in soft X-ray free-electron laser (XFEL) beamlines. Accurately evaluating the properties of grating monochromators with near-realistic XFEL pulse is of paramount importance. In this study, we utilize the start-to-end pulse propagation method to conduct a characterization of grating monochromator performance at the FEL-1 beamline of SFEL.

Genomic insights into the convergent evolution of desert adaptation in camels and antelopes.

Extreme heat and chronic water scarcity present formidable challenges to large desert-dwelling mammals. In addition to camels, antelopes within the Hippotraginae and Alcelaphinae subfamilies also exhibit remarkable physiological and genetic specializations for desert survival. Among them, the critically endangered addax ( ) represents the most desert-adapted antelope species.

Overcoming cancer treatment resistance: Unraveling the role of cancer-associated fibroblasts.

The resistance to cancer treatment is a major clinical obstacle, being strongly influenced by the tumor microenvironment (TME). Cancer-associated fibroblasts (CAFs) are critical elements of the TME. CAFs are heterogeneous and are activated through diverse pathways.

Age-period-cohort analysis of syphilis epidemics in Eastern China, China, 2005-2024.

Background: Syphilis remains one of the serious public health challenges in China and worldwide. This study aims to assess the potential independent risks associated with age, period, and birth cohort for the reported incidence of syphilis in Eastern China.

Methods: Data on all syphilis cases from 2005 to 2024 in Zhejiang Province in Eastern China were collected from the China Information System for Disease Control and Prevention.

Aberrant Notch-signaling promotes tumor angiogenesis in esophageal squamous-cell carcinoma.

Esophageal squamous-cell carcinoma (ESCC) is one of the most common gastrointestinal cancers in China, characterized by high malignancy and poor prognosis. Nowadays, the therapeutic options for this cancer are very limited. Notch-signaling is often overactivated in ESCC, but its role remains to be fully elucidated.

Interface-engineered BiO/N-doped carbon heterostructure enabling synergistic effects for advanced energy storage.

Metal oxide/carbon nanocomposites have emerged as prospective electrodes for electrochemical energy storage. In this case, revealing the synergistic mechanism of metal oxide/carbon is favorable to guide the design of nanocomposites and enhance their electrochemical performance. Thus, in this study, an interface-engineered BiO/N-doped carbon heterostructure (BiO@NPCF) was designed as a high-performance active site for K and Na storage.

Whole-genome sequencing provides insights into the evolutionary adaptation and conservation of gibbons.

Gibbons are small, arboreal apes that play a critical role in tropical biodiversity and ecosystem ecology. However, nearly all species of gibbons are threatened by habitat loss, illegal trade, hunting, and other human activities. Long-term poor understanding of their genetics and evolution undermines effective conservation efforts.

Sex-specific associations of social isolation and loneliness with residual life expectancy at age 45 years among middle-aged and older adults in China.

Background: Globally, one-third of older adults experience social isolation or loneliness, making them critical public health priorities. However, the impact of social isolation and loneliness on life expectancy remains underexplored. This study investigated the separate and joint associations of social isolation and loneliness with residual life expectancy at age 45 years, alongside the sex-specific variations in these associations with the aim of informing targeted strategies for mitigating social health disparities in rapidly ageing populations.

Image enhancement network based on fiber topology-preserving multi-module fusion for neuron reconstruction.

The rapid development of optical labeling and imaging technologies has enabled scientists to capture three-dimensional images of mammalian brains at the single-neuron level. However, it has also brought about numerous technical challenges. In neuronal images, fibers exhibit lower fluorescence intensity than cell bodies and are densely packed, making it difficult to distinguish fiber signals from background noise or resolve connectivity accurately.

Characterization of a Bifunctional Glucoamylase GA from with Dual Hydrolytic Activity on Starch and Chitosan.

Glucoamylase is essential for the hydrolysis of starch to glucose and has broad industrial applications. Although its catalytic domain shares similarities with GH8 family chitosanases, which are known for their bifunctional activity, no bifunctional glucoamylase has been reported to date. In this study, we identify and characterize GA, a glucoamylase from the pathogenic fungus 293, which exhibits a dual hydrolytic activity toward both starch and chitosan.

Spatial analysis and prediction of psittacosis in Zhejiang Province, China, 2019-2024.

Background: The emergence of advanced diagnostic techniques and improved disease surveillance have led to increased recognition of psittacosis cases in recent years. This study aimed to characterize the epidemiological patterns and spatiotemporal distribution of psittacosis in Zhejiang Province, China, and to identify high-risk clusters through predictive modeling.

Methods: We conducted a comprehensive analysis of reported psittacosis cases in Zhejiang Province from January 2019 to June 2024.