Multi-omics reveal developmental stage-specific adaptations of Tropilaelaps mercedesae to the honeybee host.

The ectoparasitic honeybee (Apis mellifera) mite Tropilaelaps mercedesae represents a serious threat to Asian apiculture and a growing concern for global beekeeping due to its high reproductive capacity and host adaptability. However, the regulatory mechanisms underlying its host adaptation across life stages remain poorly characterized. Here, we performed integrated transcriptomic, proteomic, and metabolomic analyses of female mites at 4 key postembryonic developmental stages: protonymphs, deutonymphs, mature adults, and reproductive adults.

Varroa destructor infestation amplifies imidacloprid vulnerability in Apis mellifera.

Honey bee health is affected by a variety of environmental factors, with Varroa destructor parasitism and pesticide exposure being important factors contributing to colony decline. In this study, we assessed the effects of V. destructor infestation in combination with imidacloprid exposure on honey bees.

Integrated metabolomic profiling of olive oil, olive mill wastewater, and pomace from Egyptian cultivars based on UHPLC-MS/MS and NMR coupled with chemometrics.

Introduction: Olive oil and their by-products have long been regarded as valuable sources of health-promoting metabolites. Although the composition of olive oil has been widely studied, less attention has been given to olive mill wastewater (OMWW) and pomace across different cultivars (cvs.).

Expression of Regulates Body Weight and Body Size by Targeting JH in Honeybees ().

Honeybees () are pollinators for most crops in nature and a core species for the production of bee products. Body size and body weight are crucial breeding traits, as colonies possessing individuals with large body weight tend to be healthier and exhibit high productivity. In this study, small interfering RNA (siRNA) targeting () was incorporated into the feed for feeding worker bee larvae, thereby achieving the silencing of this gene's expression.

Gene Regulation by RNAi on Apis mellifera ligustica Colonies Based on Engineering Bacteria and Nanomaterials.

Honey bees, as indispensable pollinators, can promote agricultural production and maintain ecological balance. Because of honey bees' eusociality, studying honeybee colonies is of great importance. RNA interference (RNAi) has emerged as a powerful tool for honey bee research.

Advancing antioxidant testing: Standardisation of a human plasma oxidation assay and its integration with chemical and cellular protocols.

Chemical antioxidant methods (CAM) are highly reproducible, but they fail to capture the dynamic nature of biological systems. In contrast, cellular antioxidant assays (CAA) offer a more comprehensive evaluation of antioxidant activity by assessing redox status. However, these assays are time- and resource-intensive.

Niemann-Pick type C2 protein PpseNPC2-1 binding volatile nerolidol mediates prey localization of the pond wolf spider Pardosa pseudoannulata.

As a key natural enemy of common agricultural pests, the pond wolf spider Pardosa pseudoannulata preys on planthoppers, leafhoppers and other pests. Chemoreception is essential for P. pseudoannulata in locating prey and searching for mates.

Land Use and Season Interactively Affect Honeybee () Body Size and Fat Stores.

The loss and fragmentation of habitats caused by anthropogenic activities in the last decades has affected foraging habitat quality and, therefore, foraging success (food quantity, quality and foraging range) of many animals, including many pollinators. Honeybees () are important pollinators of many plant species, and habitat change has also affected their ability to collect the resources they need to maintain the colony. Two important biological traits that might be affected by land use are body size and fat stores, which have the potential to affect body condition and therefore success and colony health.

Heat-responsive ame-miR-1-3p modulates thermotolerance in honeybees (Apis mellifera L.).

Thermal stress significantly harms the survival, physiology and development of honeybees (Apis mellifera L.). miRNAs are emerging as key regulators of environmental stress responses.

Floral scent affects bumblebee pollinator preferences on the flowers of four tomato cultivars under greenhouse conditions.

Bumblebee pollination is essential for greenhouse tomato. Floral traits, such as olfactory signal and food reward, have significant influences on pollinator foraging decisions and thus result in different pollination performance. Chemical-analytical, bioassays, and electroantennogram tests were used to determine whether these tomato Lycopersicum esculentum cultivars differed in floral scent and food reward, and how these floral traits affected bumblebee Bombus lantschouensi visitation performance.

A new cage design to test pesticide impacts on honey bees via toxicant exposure through sucrose, pollen and beeswax simultaneously.

Western honey bees (Apis mellifera) are exposed to pesticides via nectar/honey, pollen and beeswax. The impacts of honey bee exposure to pesticides via pollen or beeswax currently are not considered in pesticide risk assessments. Correspondingly, we developed an in vitro method through which one can expose adult honey bees to pesticide residues in all matrices simultaneously.

Nosema or Vairimorpha: Genomic/proteomic support to a complex socio-economic issue rooted in taxonomic change.

Microsporidia are disease-causing organisms that can infect invertebrate species. In apiculture, two microsporidians of importance are Vairimorpha (=Nosema) ceranae and Vairimorpha (=Nosema) apis. The taxonomy surrounding the genus assignment of these species has been heavily debated, due to molecular systematic and socio-economic reasons.

Diverse Sublethal Effects of a Common Fungicide Impact the Behavior and Physiology of Honey Bees.

Honey bees and other pollinators are key to functioning natural and managed ecosystems. However, their health is threatened by many factors, including pesticides. Spraying fungicides during flowering of fruit trees is widespread even though it directly exposes pollinators to these fungicides.

Dissecting the Molecular Mechanism of 10-HDA Biosynthesis: Role of Acyl-CoA Delta(11) Desaturase and Transcriptional Regulators in Honeybee Mandibular Glands.

10-Hydroxy-2-decenoic acid (10-HDA), a major fatty acid (FA) component of royal jelly, is synthesized in the mandibular glands (MGs) of worker honeybees. Despite its well-documented nutritional and therapeutic significance, the biosynthetic pathway and regulatory mechanisms of 10-HDA production remain largely unresolved. In this study, the molecular basis of 10-HDA biosynthesis and regulation in the MGs of newly emerged bees (NEBs), nurse bees (NBs), and forager bees (FBs) were investigated using RNA sequencing and weighted gene co-expression network analysis (WGCNA).

Di--Coumaroyl Spermidine From Bee Pollen Alleviates Chronic Nonbacterial Prostatitis.

Bee pollen and its extracts have been used for decades as therapeutic agents or health food supplements to alleviate chronic nonbacterial prostatitis (CNP). However, the functional compounds in bee pollen that are anti-CNP remain still unclear. In this study, we evaluated the anti-CNP properties of six principal phenolamides in bee pollen.

Unveiling the phytochemical profiles of honey: An authenticity assurance approach.

(jujube) honey, as a significantly agricultural product, faces persistent challenges in quality control due to the absence of reliable authentication techniques against adulteration. The research systematically identified and quantified phytochemicals using High-Performance Liquid Chromatography (HPLC) and High-Performance Liquid Chromatography-mass spectrometry (HPLC-MS). We identified and quantified 13 phytochemicals in the jujube honey samples, including 5 terpenoids, 4 phenolamides, 3 quinolines and 1 indole.

Advances in conventional and innovative anti-allergic agents for managing food allergies: from mechanisms to challenges.

Food allergies constitute a significant and escalating global public health issue. Over the past decade, efforts have intensified to prevent and treat these allergies, including exploring new anti-allergic agents and natural bioactive compounds with minimal side effects. Despite progress, consensus on effective strategies remains elusive.

Gut microbiota regulates the brain metabolism of sexually mature drones.

Previous studies have found that gut microbiota and metabolites may be crucial in insect sexual maturation. For example, during sexual maturity, the increased dopamine in the drone's brain can affect its reproductive behavior, such as flight, mating, and so on. In addition, gut microbiota can affect brain functions such as learning and memory through metabolites.

Characterization and feature selection of volatile metabolites in Yangxian pigmented rice varieties through GC-MS and machine learning algorithms.

Introduction: Pigmented rice is fascinated by consumers for its abundant phytochemicals and unique aroma.

Methods: In this study, GC-MS-based metabolomics of Yangxian colored rice varieties were performed to characterize their volatile metabolites through multivariate statistics and machine learning algorithms.

Results: Results showed that a total of 357 volatile metabolites were detected and segmented into 9 groups, including 96 organooxygen compounds (26.

Flupyradifurone Exhibits Greater Toxicity to the Asian Bumblebee Compared to the European Bumblebee .

Pesticides are considered a major factor in the decline of bee populations. Flupyradifurone, a novel insecticide, is believed to be relatively 'bee-safe'. This study aims to evaluate the acute and chronic toxicity of flupyradifurone and assess its risks to both commercial bumblebee and the Asian native species .

Novel strategy for screening aptamers of Staphylococcus aureus enterotoxin A based on active fragments and fusion design.

Background: Staphylococcus aureus enterotoxin A (SEA) is the major toxin responsible for food poisoning caused by Staphylococcus aureus infection. Aptamers are an ideal solution for visual detection of SEA. However, screening for aptamers is generally expensive and time-consuming.

Dissection of genetic basis underlying heat stress response of Apis cerana.

The honeybee Apis cerana as an important pollinator contributes significantly to ecological diversity. In recent years, it has been used as a common pollinator in greenhouses, but it is highly susceptible to heat stress, which affects its behavior, physiology, survival, and gene expression. Here, we conducted transcriptomic analysis to identify differentially expressed genes (DEGs) and reveal the associated biological processes in the queen head and ovary of honeybee A.

Potential Correlation Between Bombus lantschouensis Thoracic Morphology and Flight Behavior.

Remarkably little modern work has investigated the thoracic structures of insects and their relationship to flight locomotion. Most studies focus exclusively on either morphology or flight kinematics. In this study, we explore within-species variation in mesosomal structures (thorax + propodeum) and their correlation with different flight modes of Bombus lantschouensis.

Insecticide and pathogens co-exposure induces histomorphology changes in midgut and energy metabolism disorders on Apis mellifera.

Honey bees in agroecosystems face increasingly exposure to multiple stressors, such as pesticides and pathogens, making it crucial to assess their combined impacts rather than focusing on individual factors alone. This study examined the adverse effects of single exposure acetamiprid, Varroa destructor, and Nosema ceranae, both individually and in combination, on honey bee survival, midgut integrity and transcriptomic changes to understand the molecular mechanisms involved. The findings revealed that combination of acetamiprid and N.