Single-cell transcriptomic atlas of human retina from Chinese donors reveals population-specific cellular diversity.

The human retina exhibits complex cellular heterogeneity which is critical for visual function, yet comprehensive ethnic-specific references are scarce in ophthalmic transcriptomics. The lack of single-cell RNA sequencing (scRNA-seq) data from Asian populations particularly Chinese donors imposes significant limitations in understanding population-specific retinal biology. We constructed the first comprehensive single-cell transcriptomic atlas of the human retina from Chinese donors, generated through high-throughput scRNA-seq of ∼290,000 viable cells obtained from 18 fresh retinal specimens (living donor and post-mortem specimens).

Association and mechanism of Ligusticum chuanxiong alleviating itch: A combination of experimental and network pharmacology study.

Ethnopharmacological Relevance: Ligusticum chuanxiong (CX) is a traditional Chinese medicine with diverse effects, such as anti-inflammatory, antioxidant, neuroprotective, anti-nociceptive, and anticancer properties. It has been used in clinical applications with satisfying therapeutic effects. However, the underlying mechanism that contributes to the anti-pruritic effects remains to be comprehensively investigated.

Performance comparison of germline variant calling tools in sporadic disease cohorts.

Accurate variant calling is essential for next-generation sequencing (NGS)-based diagnosis of rare diseases, yet most benchmarking studies have focused on standard cell lines or trio-based samples, with limited relevance to sporadic cases. Here, we systematically compared the performance of DeepVariant and GATK HaplotypeCaller in two Chinese cohorts of patients with sporadic epilepsy (EP) and autism spectrum disorder (ASD). DeepVariant exhibited higher precision and sensitivity in detecting single nucleotide variants (SNVs), while GATK showed a distinct advantage in identifying rare variants, which are often key to understanding the genetic basis of rare diseases.

Haemodynamic characteristics of thin-walled regions in intracranial aneurysms: intraoperative imaging and CFD analysis.

Background: Identifying haemodynamic factors associated with thin-walled regions (TWRs) of intracranial aneurysms is critical for improving pre-surgical rupture risk assessment. Intraoperatively, these regions are visually distinguished by a red, translucent appearance and are considered highly rupture prone. However, current imaging modalities lack the resolution to detect such vulnerable areas preoperatively.

The insight in wheat starch entanglement behavior and its effect on starch gel in aqueous solution.

The objective of this study is to investigate the behavior of starch entanglement and to elucidate the starch gelation process from the entanglement perspective. This study established a starch entanglement system based on overlap, entanglement, and concentrated concentrations in aqueous solution. Select the temperature at which starch is prone to form gel (4 °C) and the temperature at which starch begins to gelatinize (60 °C) for entanglement.

Multimodal deep learning methods for speech and language rehabilitation: a cross-sectional observational study.

The speech and language rehabilitation are essential to people who have disorders of communication that may occur due to the condition of neurological disorder, developmental delays, or bodily disabilities. With the advent of deep learning, we introduce an improved multimodal rehabilitation pipeline that incorporates audio, video, and text information in order to provide patient-tailored therapy that adapts to the patient. The technique uses a cross-attention fusion multimodal hierarchical transformer architectural model that allows it to jointly design speech acoustics as well as the facial dynamics, lip articulation, and linguistic context.

Metal ions and colorectal cancer: unlocking the secrets of tumour cell death.

Background: Colorectal cancer (CRC) is a highly prevalent and fatal malignancy worldwide. Despite advancements in early screening techniques and treatments, the prognosis for patients remains suboptimal. Studies have shown that metal ions play crucial roles in the occurrence, progression, and treatment of CRC.

The gut microbiome in lung cancer: from pathogenesis to precision therapy.

The gut microbiome has emerged as a key modulator of immune responses and treatment efficacy in oncology. Growing evidence links gut dysbiosis to resistance against immune checkpoint inhibitors (ICIs) in advanced cancers, prompting exploration of the gut-lung axis-a bidirectional network connecting intestinal microbiota with pulmonary health. Given lung cancer's status as the leading cause of cancer mortality worldwide, understanding this axis holds significant therapeutic potential.

High-Temperature Superconductivity of Thermodynamically Stable Fluorite-Type Hydrides at Ambient Pressure.

Hydride superconductors have attracted significant attention, yet achieving superconductivity at ambient pressure remains a key challenge. Here, a family of high-T (superconducting critical temperature, T) hydrides based on the fluorite-type AXH structure, exhibiting thermodynamic and dynamic stability at low to atmospheric pressure, is proposed. Through comprehensive screening of 150 ternary systems, eight stable hydrides below 35 GPa are identified.

Unveiling the impact of adherence: imatinib plasma levels and survival in postoperative gastrointestinal stromal tumor (GIST) patients.

Background: Adherence to imatinib may be even more limited in the adjuvant setting, as patients receiving adjuvant imatinib often do not experience disease symptoms after tumor removal. This real-world study aimed to gain insight into adherence to imatinib and the effect of adherence on treatment outcomes.

Methods: Postoperative GIST patients who visited the speciality clinic between January 2021 and September 2024 were included in the study.

ClC-3 inhibition induces autophagy to reverse cisplatin resistance in cervical cancer via the Akt/mTOR pathway.

Cervical cancer is one of the most prevalent types of cancer among women. Nowadays, surgery is still the primary treatment for cervical cancer. Cisplatin was regarded as the standard medication for non-surgical therapy.

A Synergistic Role of Photosynthetic Bacteria and Fungal Community in Pollutant Removal in an Integrated Aquaculture Wastewater Bioremediation System.

This study addresses the understanding of fungal diversity and their bioremediation roles in an integrated aquaculture wastewater bioremediation system, an area less explored compared to bacteria, viruses, and protozoa. Despite the rapid advancement and affordability of molecular tools, insights into fungal communities remain vague, and interpreting environmental studies in an ecologically meaningful manner continues to pose challenges. To bridge this knowledge gap, we developed an integrated aquaculture wastewater bioremediation system, incorporating photosynthetic bacteria, and utilizing internal transcribed spacer (ITS) sequencing to analyze fungal community composition.

Late retroperitoneal recurrence of adult-type ovarian granulosa cell tumors: Two case reports and literature review.

Adult-type ovarian granulosa cell tumors are rare low-grade malignancies characterized by late recurrence and atypical clinical manifestations. This study reports two cases of recurrent adult-type ovarian granulosa cell tumor with distinct timelines and anatomical sites, including one case with two postoperative recurrences. We analyze their imaging features, surgical management, histopathology, postoperative follow-up, and review relevant literature.

SrZnSiS: Shattering the 4.0 eV Threshold toward Dual-Optimized Bandgap and Second-Harmonic Generation.

Infrared (IR) nonlinear optical (NLO) crystals are crucial for mid-IR lasers, yet their intrinsic narrow band gaps cause low laser-induced damage thresholds (LIDTs) and detrimental two-photon absorption, thus limiting their widespread application. Herein, a synergistic strategy involving d-orbital exclusion and polarization motif construction is employed to synthesize novel chalcogenide SrZnSiS by introducing [SiS] and [ZnS] tetrahedral polarization subunits. Performance evaluation demonstrates that SrZnSiS breaks through the "4.

Machine Learning-Based Prediction of Lymph Node Metastasis and Volume Using Preoperative Ultrasound Features in Papillary Thyroid Carcinoma.

Objective: A predictive model of cervical lymph node metastasis and metastasis volume was constructed based on a machine learning algorithm and ultrasound characteristics before surgery.

Methods: A retrospective analysis was conducted on 573 cases of PTC patients who underwent surgery in our institution, from 2017 to 2022. Patient demographic and clinical characteristics were systematically collected.

Diffuson-Dominated Thermal Transport Crossover From Ordered to Liquid-Like CuBiS: The Negligible Role of Ion Hopping.

Fundamentally understanding lattice dynamics and thermal transport behavior in liquid-like, partially occupied compounds remains a long-standing challenge in condensed matter physics. Here, the microscopic mechanisms are investigated underlying the ultralow thermal conductivity in ordered/liquid-like CuBiS by combining experimental methods with first-principles calculations. The ordered structure and liquid-like are first experimentally synthesized and characterized, partially Cu-atom occupied CuBiS structure with increasing temperature.

Rapid and simple detection of using real closed dumbbell-mediated isothermal amplification.

Introduction: () is a well-known widespread food-borne pathogen that poses a threat to public health. Suitable detection methods are needed to effectively control and prevent pathogenic infections.

Methods: This study aimed to develop a novel closed dumbbell-mediated isothermal amplification (CDA)-based assay to achieve rapid and simple detection of The newly developed CDA technology is capable of amplifying DNA targets with high sensitivity and specificity.

"One Rocket, Multiple Satellites": Natural Small Molecule Nanocarriers With In-Situ Nanosphere-to-Nanofiber Transition for Combined Chemo/PDT/Anti-Angiogenic Antitumor Therapy.

Drug delivery based on dynamic morphological transformation represents the cutting-edge strategy at the forefront of chemotherapy, integrating individual advantages that depend on the delivery stage and spatially resolved morphology. However, developing a tumor-specific, in vivo morphologically transformable small-molecule drug delivery system to achieve enhanced drug accumulation and photodynamic therapy (PDT) efficacy remains challenging. In this study, a multifunctional PDT formulation using natural small molecules as nanocarriers is developed, combining a tumor-targeting photosensitizer and anti-angiogenic therapy.

Primary hepatic neuroendocrine neoplasms: a case series of 10 patients and literature review.

The clinical data of 10 patients with pathologically confirmed primary hepatic neuroendocrine neoplasms from Ningbo medical institutions between 2012 and 2024 were retrospectively analyzed. The cohort included 8 males and 2 females, with a median age of 63 years. None presented with carcinoid syndrome.

FBXO9 mediated the ubiquitination and degradation of YAP in a GSK-3β-dependent manner.

The Hippo signaling pathway effector YAP (Yes-associated protein) serves as a critical transcriptional regulator involved in a wide range of biological processes, including oncogenesis. Despite its potential as a therapeutic target, pharmacologically targeting the Hippo/YAP axis remains challenging, necessitating further exploration of the mechanisms governing YAP regulation. In this study, we identify the Cullin-RING E3 ligase complex SCF-FBXO9-CRL1 as a novel posttranslational regulator of YAP stability.

Melanin metabolism mediated via tyrosinase protein involved in red shell coloration of hard clam, Meretrix meretrix.

Molluscan shell color polymorphism is influenced by a variety of factors, and endocrine pigments secreted by mollusks play a crucial role in determining shell coloration. The hard clam (Meretrix meretrix) exhibits one of the most extensive variations in shell color among molluscan species, and the mechanism underlying its shell pigmentation remains largely unexplored. The stable inheritance of red shell color in a newly identified variant of M.

CAFs promote immune evasion in gastric cancer through histone lactylation-mediated suppression of NCAPG ubiquitination.

Background: Cancer-associated fibroblasts (CAFs) can facilitate tumor progression through multiple approaches. Research indicates that CAFs in various tumors exhibit robust lactate metabolism, ultimately becoming the primary source of lactate in the tumor microenvironment. Emerging evidence has established that CAFs could orchestrate gastric cancer (GC) immune evasion.

Mobile App-Assisted Self-Monitoring of Blood Glucose in Type 2 Diabetes in Ningbo, China: 12-Month Retrospective Cohort Study.

Background: Self-monitoring of blood glucose (SMBG) is recommended in clinical practice guidelines, including those in China, as part of patient education, self-management, and empowerment. With technological advancements, telecommunication technologies are now used for telemonitoring in health care. Mobile apps have become a practical tool for SMBG among patients with type 2 diabetes mellitus (T2DM).