ClC-3 inhibition induces autophagy to reverse cisplatin resistance in cervical cancer via the Akt/mTOR pathway.

Cervical cancer is one of the most prevalent types of cancer among women. Nowadays, surgery is still the primary treatment for cervical cancer. Cisplatin was regarded as the standard medication for non-surgical therapy.

Case Report: Dramatic response to entritinib in a patient with gastrointestinal stromal tumor positive for fusion.

Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the gastrointestinal tract, with proto-oncogene, receptor tyrosine kinase (c-kit), or PDGFRα mutations detected in around 85% of cases. GISTs without c-kit or platelet-derived growth factor receptor alpha (PDGFRα) mutations are considered wild-type (WT). Recently, some molecular alterations, including neurotrophic tyrosine receptor kinase (NTRK) fusions, have been reported in very few cases of WT GISTs.

Nano-enabled strategies for targeted immunotherapy in gastrointestinal cancers.

Gastrointestinal (GI) cancers remain a leading cause of global cancer morbidity and mortality, demanding novel therapeutic strategies that overcome existing limitations. Nanomedicine has recently emerged as a transformative approach, offering the potential to significantly enhance immunotherapy outcomes through precision targeting and modulation of tumour immune microenvironments. This review discusses the principal categories of precision-engineered nanoparticles-including lipid-based carriers, polymeric systems, protein-derived formulations, and metallic-hybrid composites-emphasising their capacity for targeted immune modulation and improved pharmacokinetic profiles.

[Study on the analgesic effect of electroacupuncture at different intensities on the primary input level in mice with inflammatory muscle pain based on calcium imaging].

Objectives: To observe the differential analgesic effects of electroacupuncture (EA) at different intensities on calcium activity in dorsal root ganglia (DRG) neurons induced by mechanical, thermal, and cold stimuli in a state of muscular inflammatory pain, so as to preliminarily reveal the peripheral integration characteristics of acupuncture analgesia mechanisms.

Methods: The inflammatory muscle pain model was established by injection of complete Freund's adjuvant (CFA) into the right tibialis anterior muscle. EA (0.

A hydrogel tissue adhesive incorporating basic fibroblast growth factor-loaded liposomes accelerates cutaneous wound healing by enhancing cell proliferation, collagen synthesis and angiogenesis.

Tissue adhesives have become substitutes or adjuvants for surgical sutures owing to their minimal tissue damage and ease of application. However, limitations remain for existing tissue adhesives, such as weak adhesion strength, potential toxicity, and lack of bioactivities to promote wound healing. Here, we developed an injectable and biocompatible hydrogel tissue adhesive incorporating basic fibroblast growth factor (bFGF)-loaded liposomes for sutureless wound closure and promoting wound healing.

L. fruit as potential functional ingredient: From optimized extraction to biological activities evaluation.

L ( L.) is a medicine and food homologous plant, whose fruit is acknowledged by the International Health Organization for its high nutritional value. However, eco-friendly and highly efficient methods for extracting the active components from L.

Tracking symptom dynamics in people living with HIV on different treatment: a longitudinal cross-lagged panel network analysis.

Objective: To characterize dynamic interrelationships among physical, cognitive, and psychological symptoms in people living with HIV and identify bridge symptoms between subgroups receiving distinct antiretroviral therapy regimens.

Methods: A longitudinal design was employed in this study. A total of 676 individuals diagnosed with HIV filled out the Self-Report Symptom Checklist (HIV-SRSC) at baseline, 3-month, and 6-month follow-ups in the clinic.

Design and computational optimization of a laser communication adaptive optics wavefront processor based on ARM architecture.

The adaptive optics wavefront processor is essential in laser communication, enabling real-time correction of wavefront distortions to improve signal quality. With increasing demand for miniaturized, low-power, high-speed processors in mobile platforms, this study designs and optimizes a wavefront processor based on ARM architecture. The research leverages ARM's low-power and computational efficiency advantages to enhance wavefront computation speed.

Comparative plastome evaluation, phylogenomic analysis, and DNA signatures of medicinally important malvaceous genera and their adulterants.

The Bala group of plants, classified under the Malvaceae family, is acknowledged in the Ayurvedic Pharmacopoeia of India and is well-known for its medicinal uses, especially in boosting vitality and addressing neurological and cardiovascular conditions. The key genera of this group, such as Sida and Abutilon, are highly valued in traditional medicine; however, the efficacy of herbal formulations is frequently compromised by adulterants from morphologically similar genera like Malvastrum and Malva. To address this critical challenge, our study focuses on plastome sequencing of various Bala group taxa and their adulterants, aiming to delineate DNA signatures for key species and provide a robust strategy against adulteration.

Predicting Quality of Life in People Living with HIV: A Machine Learning Model Integrating Multidimensional Determinants.

Objective: With survival steadily improving among people living with HIV(PLWH), quality of life (QoL) has emerged as the ultimate benchmark of therapeutic success. We therefore aimed to develop and validate machine learning models that predict QoL trend in PLWH, identifying key determinants to inform personalized interventions and optimize long-term well-being.

Methods: In this longitudinal observational study, PLWH were recruited from March 2024 to December 2024.

Evaluating the Diagnostic and Prognostic Value of Peripheral Immune Markers in Glioma Patients: A Prospective Multi-Institutional Cohort Study of 1282 Patients.

Objective: Glioma is the most common primary brain tumor, with a specific immune microenvironment and aggressive nature. Novel systemic immune-inflammation indices (nSII) are the most comprehensive non-invasive biomarkers that represent patients' peripheral immune status, which are urgently needed to improve clinical management. However, the diagnostic and prognostic value of nSII in glioma remains unknown.

Efficacy and safety of Shou Hui Tong Bian capsules in the treatment of functional constipation in middle-aged and elderly patients: A multicenter, randomized, controlled trial.

Ethnopharmacological Relevance: Shou Hui Tong Bian Capsules, rooted in traditional Chinese medicine (TCM), are designed to treat functional constipation (FC) by balancing Yin and Qi, which are often disrupted in middle-aged and elderly individuals. These capsules contain a combination of herbs historically used in TCM to promote digestive health and improve bowel movement regularity, providing a potential alternative to conventional treatments.

Aim Of The Study: The aim of this study was to evaluate the efficacy and safety of Shou Hui Tong Bian Capsules in treating functional constipation in middle-aged and elderly patients, compared with a conventional lactulose oral solution.

Optimization of ultrasound enzyme-assisted trehalose lipid extraction of Coptis alkaloids and evaluation of its anti-inflammatory effects.

Coptidis Rhizoma (CR), a traditional Chinese medicine, has extensive pharmacological activity because it is rich in isoquinoline alkaloids. Nonetheless, the limited solubility of these alkaloids presents a significant challenge, hindering the full realization of CR's therapeutic potential through conventional extraction methods. To address this issue and enhance the solubility of the target compounds while optimizing extraction efficiency, this study employed an environmentally sustainable extraction technique, Ultrasound-Enzyme-Trehalose Lipid (UET), to synergistically extract five key alkaloids from CR.

Investigating inhibitory control and cognitive flexibility in self-limited epilepsy with centrotemporal spikes: an eye-tracking study.

Objectives: This study employed high-precision infrared eye-tracking technology to systematically investigate domain-specific impairments in executive function components-specifically inhibitory control and cognitive flexibility-among children with Self-Limited Epilepsy with Centrotemporal Spikes (SeLECTS).

Methods: Thirty-six children diagnosed with SeLECTS (age 11.6 ± 2.

[Study on the underlying autonomic nervous mechanism of electroacupuncture in improving colonic inflammation in mice with chronic ulcerative colitis].

Objectives: To observe the effect of electroacupuncture (EA) at "Tianshu" (ST25) on colonic inflammatory response in mice with ulcerative colitis (UC), so as to explore its underlying mechanisms in anti-inflammatory.

Methods: In the first part of this study, 36 male C57BL/6J mice were randomly divided into normal, model, and EA groups (12 mice/group) according to their body weight. The UC mouse model was established by 3% dextran sulfate sodium (DSS) solution by free drinking for 7 consecutive days.

Genome-based analyses from four clinically-isolated strains refined the taxonomy of Proteus genomosp. 6 and revealed their underestimated role in gastrointestinal diseases.

Background: Proteus spp. have long been recognized for their role in urinary tract infections, while recent evidence disclosed their implications in gastrointestinal diseases. Despite this, the taxonomy of clinically-derived Proteus spp.

Effect of emodin on Streptococcus suis by targeting β-ketoacyl-acyl carrier protein synthase Ⅱ.

Background: Streptococcus suis is a zoonotic pathogen that causes meningitis, septicaemia, endocarditis, arthritis, and pneumonia in human beings. With the increasing prevalence of S. suis infections and a general decline in the effectiveness of antibiotics, the development of novel drugs that have effect on S.

Gut microbiota and gastrointestinal tumors: insights from a bibliometric analysis.

Introduction: Despite the growing number of studies on the role of gut microbiota in treating gastrointestinal tumors, the overall research trends in this field remain inadequately characterized.

Methods: A bibliometric analysis was conducted using publications retrieved from the Web of Science Core Collection (up to September 30, 2024). Analytical tools including VOSviewer, CiteSpace, and an online bibliometric platform were employed to evaluate trends and hotspots.

[Effect of electroacupuncture on the primary input level of dorsal root ganglion neurons under different stimuli observed by calcium imaging].

Objectives: To observe the effect of electroacupuncture (EA) on the neuronal activity of primary afferent dorsal root ganglion (DRG) caused by different mechanical stimuli such as brush, clamp [clamping hind paw or "Zusanli" (ST36) acupoint] or stimulation at different temperatures (43 ℃, 51 ℃, 4 ℃), so as to explore the interaction between EA signals and different primary afferent signals at the DRG level.

Methods: The strength threshold of activated A fiber was determined by electrophysiological technique. DRG neurons were labeled by intrathecal injection of AAV9 virus, and the response of DRG neurons to different primary afferent stimuli was observed and recorded by calcium imaging.

[Bidirectional regulation of distal colon motility in rats with electroacupuncture of different intensities at "Tianshu" (ST25)].

Objective: To observe the distribution characteristics of sensitization areas on the body surface in the rat models with functional constipation and diarrhea, explore the regulatory patterns of electroacupuncture (EA) of different intensities at "Tianshu" (ST25) on distal colon motility, and clarify the roles of the neurons of different subtypes in the enteric nervous system (ENS) displayed in the regulatory effect.

Methods: Of 90 SD male rats of SPF grade, 15 rats were randomized into a normal group, a constipation group and a diarrhea group, 5 rats in each one. The stool form and fecal water content, as well as the distribution of the Evans blue (EB) extravasation on the body surface after the intravenous injection with EB on the tails were observed.

KunMingShanHaiTang formula reprograms macrophage metabolism and promotes M2 polarization via the HIF-1α pathway to alleviate ulcerative colitis symptoms in a rat model.

The KunMingShanHaiTang Formula (KMSHTF), adjusted by Professor Zhong Chuanhua for the treatment of ulcerative colitis (UC), is the work of a renowned veteran practitioner of Chinese medicine. However, its specific mechanism remains unknown. Consequently, it is intriguing to investigate the molecular mechanism by which KMSHTF treats UC.

Exploring the regulatory role of CNPY3 as a prognostic biomarker on human glioma cell migration, invasion and immune infiltration.

BackgroundCanopy FGF signalling regulator 3 (CNPY3) is involved in immune regulation, tumorigenesis and development, nevertheless, its role in glioma remains largely unexplored. Our study aimed to explore the regulatory role of CNPY3 as a prognostic biomarker in human glioma cell migration, invasion and immune infiltration.MethodsBioinformatics analysis of CNPY3 and clinical relevance of glioma in public databases was performed.

Optimization ultrasonic-assisted aqueous two-phase extraction of glabridin from licorice root and its activity against the foodborne pathogen MRSA.

This study aimed to extract glabridin (GLA) from licorice using an environmentally sustainable ultrasonic-assisted aqueous two-phase extraction method and to evaluate its efficacy. The extraction parameters were optimized through single-factor experiments and response surface methodology, resulting in a GLA content of 2049.51 μg/g under the conditions of 51 min ultrasonic time, 76 °C ultrasonic temperature, and 640 W ultrasonic power.

[Analgesic effects of electroacupuncture at different intensities on visceral pain in rats].

Objectives: To observe the analgesic effect of electroacupuncture (EA) at different intensities on visceral pain in rats and explore the supraspinal central mechanism and dose-response relationship.

Methods: Male SD rats were randomly divided into a normal group, a model group and the groups of EA at different intensities (0.5, 1, 2, 3 and 6 mA, 6 rats/group).