Effects of fermented feed on lipid composition and flavor of pasteurized egg yolks: A promising approach for flavor-enhanced liquid duck eggs.

This study investigated the effect of fermented feed on the lipid composition and volatile flavor compounds of pasteurized duck egg yolks using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry and lipidomics. Fermented feed significantly reduced off-odor intensity of egg yolks, as confirmed by sensory evaluation, compared to the control group. Ninety-four volatile compounds were identified, with thirty-two showing significant differences between the two groups (p < 0.

Deciphering the Gene Expression and Alternative Splicing Basis of Muscle Development Through Interpretable Machine Learning Models.

In chickens, meat yield is a crucial trait in breeding programs. Identifying key molecular markers associated with increased muscle yield is essential for breeding strategies. This study applied transcriptome sequencing and machine learning methods to examine gene expression and alternative splicing (AS) events in muscle tissues of commercial broilers and local chickens.

Gut microbiota dysbiosis exacerbates polystyrene microplastics-induced liver inflammation via activating LPS/TLR4 signaling pathway in ducks.

Ubiquitous microplastics can bioaccumulate in organisms, resulting in detrimental health impacts, such as liver inflammation. Nonetheless, the exact mechanism by which polystyrene microplastics (PS-MPs) trigger liver inflammation via the gut-liver axis in ducks remains unclear. The purpose of this study was to clarify the impact of PS-MPs exposure to liver inflammation through the gut-liver axis in ducks.

Reduced Dietary Protein Levels Improved Growth Performance, Promoted Efficient Nutrient Utilization, Increased Fecal , and Reduced Fecal Malodorous Compounds in Late-Fattening Barrows.

The objective of this study was to evaluate the effect of reducing dietary protein levels on barrows during the late fattening period. Fifty Duroc × Landrace × Yorkshire barrows with body weights averaging 76.30 ± 6.

Dietary fiber enhances milk yield in plateau dairy cows via activation of the rumen microbiota-mammary gland axis.

Milk yield in high-altitude regions such as the Qinghai-Tibet Plateau is low due to hypoxic stress and impaired mammary gland function. This study aims to determine whether a fiber-supplemented diet could increase milk yield in plateau dairy cows through modulating rumen microbiota and downstream metabolic signaling. Holstein cows were assigned to diets containing either or an aquatic plant with a high neutral and acid-detergent fiber content.

Effects of rhizoma meal supplementation on productive performance, egg quality, egg nutritional value, and serum biochemical parameters of laying ducks.

Introduction: The rhizoma of (D. Don) Hara, a traditional natural medicinal herb with extensive historical applications in China, possess anti-inflammatory, anticancer, antioxidant, antimicrobial, immunomodulatory, and antidiabetic effects. However, the potential positive effects of rhizoma meal (FDRM) on productive performance in high-density laying duck farming remain unclear.

Prevalence and antimicrobial resistance of isolated from local pig breeds in Jiangxi Province, China.

Introduction: Jiangxi Province possesses abundant genetic resources of local pig breeds, whose effective conservation is essential for maintaining biodiversity and sustainable utilization. is an important zoonotic pathogen that continuously threatens swine production systems and public health globally. This study aimed to investigate the epidemiological characteristics of among local pig breeds in Jiangxi Province.

Investigating the effect of carboxymethyl chitosan on the gel properties of chicken myofibrillar protein and its ability to adsorb flavor compounds.

This study investigated the effects of carboxymethyl chitosan (CMC) on myofibrillar protein (MP) gel properties, digestibility, and flavor-binding capacity. Results demonstrated that CMC significantly enhanced gel strength (106.51 g), water-holding capacity (77.

Unveiling the impact of muscle fiber composition on taste and aroma compounds in Jinhua pig skeletal muscles.

Muscle fiber types critically influence pork flavor by modulating taste-active and aroma compounds. This study analyzed three muscles in Jinhua pigs-longissimus dorsi (LD, predominantly type IIb fibers), serratus ventralis (SV, mainly type I/IIa), and quadriceps femoris (QF)-revealing distinct flavor profiles. SV exhibited significantly higher free amino acids (FAAs), including umami (Asp, Glu), sweet (Ala, Gly, etc.

Intramuscular iron supplementation enhances intestinal barrier function in weaned piglets challenged with enterotoxigenic .

Background: Since iron is an essential mineral for both host and microbial communities, how to scientifically replenish the iron in the context of bacterial infection has become a critical issue. The aim of this study was to compare the influence of intramuscular and oral iron supplementation on the progression of bacterial infection.

Methods: Weaned piglets served as an experimental model for iron supplementation following enterotoxigenic (ETEC) K88 infection.

Beta-Sitosterol Enhances Classical Swine Fever Virus Infection: Insights from RNA-Seq Analysis.

Beta-sitosterol (BS), a naturally occurring phytosterol abundant in plants, has been reported to exhibit diverse biological activities, including immunomodulatory and antiviral effects. Classical swine fever virus (CSFV), a member of the Pestivirus genus, remains a persistent threat to the swine industry worldwide, causing considerable economic damage. Our research found that BS significantly enhances the replication of both the CSFV-Shimen strain and the attenuated C-strain vaccine virus in PK-15 cells.

Development and Application of a Multiplex Real-Time TaqMan qPCR Assay for the Simultaneous Detection of African Swine Fever Virus, Classical Swine Fever Virus, Porcine Reproductive and Respiratory Syndrome Virus, Pseudorabies Virus, and Porcine Circovirus Type 2.

Since its emergence in China in 2018, African swine fever virus (ASFV) has posed a severe threat to the pig farming industry due to its high transmissibility and mortality rate. The clinical signs of ASFV infection often overlap with those caused by other swine viruses such as classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), and porcine circovirus type 2 (PCV2), making timely and precise diagnosis a considerable challenge. To address this, we established a TaqMan-based multiplex real-time quantitative PCR (qPCR) assay capable of simultaneously detecting ASFV, CSFV, PRRSV, PRV, and PCV2.

Investigating the correlation between phenotype, cecal microbiome, and serum metabolome in laying ducks with different shell strength.

Eggshell quality plays a critical role in the poultry industry, influencing both biological and economic outcomes. However, the factors determining eggshell strength in egg-laying ducks remain poorly understood. This study sought to explore phenotypic variations, gut microbiota composition, and serum metabolites in ducks with differing eggshell strengths to identify key microbial and metabolic factors affecting shell integrity.

Development of a novel nanobody-fused flagellin adjuvant to enhance immunogenicity in a PCV2 subunit vaccine.

Porcine circovirus type 2 (PCV2) infection causes significant economic losses in the global swine industry. The capsid (Cap) protein serves as the core antigen in PCV2 subunit vaccines, but its weak immunogenicity necessitates adjuvants to enhance the induced immune responses. Bacterial flagellin proteins are ideal candidates for next-generation vaccine adjuvants.

Research Progress on Multiplexed Pathogen Detection Using Optical Biosensors.

The rapid and precise identification of multiple pathogens is critical for ensuring food safety, controlling epidemics, diagnosing diseases, and monitoring the environment. However, traditional detection methods are hindered by complex workflows, the need for skilled operators, and reliance on sophisticated equipment, making them unsuitable for rapid, on-site testing. Optical biosensors, known for their rapid analysis, portability, high sensitivity, and multiplexing capabilities, offer a promising solution for simultaneous multi-pathogenic identification.

Polystyrene microplastics exacerbate mitophagy through mitochondrial dysfunction in the duck lung.

Microplastics (MPs) are widespread environmental pollutants that can enter the human body through the food chain, potentially leading to lung damage. However, the underlying mechanisms responsible for this damage remain unclear. Ducks, a commonly consumed poultry species in China, are particularly susceptible to MPs exposure due to their farming environment.

Influence of fermented feed on lipid composition and volatile flavor profile of duck eggs: Insights from multi-omics analysis.

This study employed a multi-omics approach to examine the impact of feed fermentation on the lipid profile and volatile flavor of duck eggs. Sensory evaluation and GC × GC-TOFMS analyses demonstrated that microbial fermentation in feed significantly reduced the off-odor of duck eggs. Among the thirty-nine differential volatile compounds identified, six-namely 3-methylbutanal, 1-octen-3-ol, hexanal, acetophenone, 2-heptanone, and 2-pentylfuran-were likely responsible for the alteration in yolk aroma.

Structural Variations Associated with Adaptation and Coat Color in Qinghai-Tibetan Plateau Cattle.

Structural variations (SVs) play crucial roles in the evolutionary adaptation of domesticated animals to natural and human-controlled environments, but SVs have not been explored in Tibetan cattle, which recently migrated and rapidly adapted to the high altitudes of the Qinghai-Tibetan Plateau (QTP). In this study, a de novo chromosome-level genome assembly for Tibetan cattle is constructed. It is found that using a lineage-specific reference genome significantly increased variant detection accuracy and completeness.

Inhibitory effect of curcumin on duck tembusu virus.

Duck Tembusu virus (DTMUV) is a significant etiological agent responsible for egg production decline in laying ducks and retarded growth in ducklings, contributing to considerable economic losses in the poultry sector. Current research mainly focuses on the development of vaccines for the prevention of DTMUV. However, there is relatively little research on antiviral drugs against this virus.

Association of FOXO3 gene polymorphisms with egg-laying intervals in pigeon.

The egg-laying interval reflects the laying performance of pigeons and is affected by the mechanism of ovulation. The Forkhead box O3 (FOXO3) gene is closely associated with follicular development, playing roles in the regulation of follicular granulocyte proliferation, differentiation and periodic ovulation. The objective of this research was to analyze associations of polymorphisms of FOXO3 gene with egg-laying intervals in pigeon.

Rapid detection of avipoxvirus using a fluorescent probe-based multienzyme isothermal amplification assay.

Avipoxvirus (APV) is a prevalent DNA virus in avian species, causing clinical symptoms of fowlpox and leading to reduced egg production, slower broiler growth, and increased mortality. The spread of APV poses a significant threat to the global poultry industry, potentially causing substantial economic losses. Effective control of APV, particularly its major species such as fowlpoxvirus and pigeonpoxvirus, requires the development of rapid and specific diagnostic tools.

Identification of functional SNP associated with sperm quality in porcine that contributes to the growth of immature Sertoli cell.

AnnexinA5 (ANXA5) has been identified as a positional candidate gene for reproduction and fertility traits in boars, but its role in testicular tissue development, as well as genetic variations remain unclear. The aim of this study was to explore the effect of ANXA5 in the growth of swine Sertoli cells and identify its functional variations. Firstly, the expression of porcine ANXA5 in different tissues was detected by semi-quantitative RT-PCR and its effect on the proliferation of Sertoli cells was evaluated by CCK8, EdU, flow cytometry analyses and qRT-PCR.

Transcriptomic Profiling of Hypoxia-Adaptive Responses in Tibetan Goat Fibroblasts.

The Tibetan goat () exhibits remarkable adaptations to high-altitude hypoxia, yet the molecular mechanisms remain unclear. This study integrates RNA-seq, WGCNA, and machine learning to explore gene-environment interactions (G × E) in hypoxia adaptation. Fibroblasts from the Tibetan goat and Yunling goat were cultured under hypoxic (1% O) and normoxic (21% O) conditions, respectively.

MicroRNA warfare: how chickens combat with gga-miR-2954.

is a member of complex and an emerging zoonotic pathogen. As part of the lymphatic system, the spleen plays a pivotal role in destroying invading pathogens. Various microRNAs (miRNAs) are involved in host resistance to pathogens.

GPCPD1 is regulated by METTL3/IGF2BP3 dependent mA modification to affect pig muscle fiber type conversion.

The N6-methyladenosine (mA) modification is the most prevalent and abundant RNA modification in eukaryotes. In our previous study, we identified that the glycerophosphocholine phosphodiesterase 1 (GPCPD1) gene was differentially expressed and diverse mA modificated in the pig soleus and extensor digitorum longus muscles. In this study, we further investigated the function of GPCPD1 gene in pig muscle development.