Genome-wide iterative fine-mapping for non-Gaussian phenotypes.

Fine-mapping seeks to identify causal variants associated to a phenotype of interest. Fine-mapping searches through genomic regions previously identified by single marker analysis of genome-wide association studies (GWAS) data. This two-stage approach (1) often fails to identify causal variants with smaller effect sizes, and (2) does not properly correct for multiple comparisons.

Injectable ROS/pH-Responsive Hydrogel for Synergistic Chemo-Photodynamic Tumor Therapy and Enhanced Wound Healing.

Conventional cancer treatments often result in tissue loss, incomplete tumor excision, infections, and systemic toxicity, severely impacting patient recovery. These complications frequently involve excessive reactive oxygen species (ROS) generation and persistent inflammation, pathological features also characteristic of burn wounds and postoperative tumor sites. To address this, an injectable ROS/pH-responsive hydrogel integrating localized tumor therapy and wound healing is developed.

Development and Application of a Multiplex Real-Time TaqMan qPCR Assay for the Simultaneous Detection of African Swine Fever Virus, Classical Swine Fever Virus, Porcine Reproductive and Respiratory Syndrome Virus, Pseudorabies Virus, and Porcine Circovirus Type 2.

Since its emergence in China in 2018, African swine fever virus (ASFV) has posed a severe threat to the pig farming industry due to its high transmissibility and mortality rate. The clinical signs of ASFV infection often overlap with those caused by other swine viruses such as classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), and porcine circovirus type 2 (PCV2), making timely and precise diagnosis a considerable challenge. To address this, we established a TaqMan-based multiplex real-time quantitative PCR (qPCR) assay capable of simultaneously detecting ASFV, CSFV, PRRSV, PRV, and PCV2.

Enhancing the quality management of hospital environmental surfaces through the application of a multidisciplinary team model.

Nosocomial infections have become one of the major causes of mortality among hospitalized patients. To improve the quality of cleaning and disinfection for hospital environmental surfaces, we conducted a multidisciplinary team (MDT) intervention study with a non-randomized control group in a general hospital in China. The MDT comprised members with educational backgrounds in nursing, clinical medicine, public health, or laboratory science.

What's in a cue?: Using natural language processing to quantify content characteristics of episodic future thinking in the context of overweight and obesity.

Episodic future thinking (EFT), an intervention in which participants vividly imagine their future, has been explored as a cognitive intervention to reduce delay discounting and decrease engagement in harmful health behaviors. In these studies, participants generate text descriptions of personally meaningful future events. The content of these text descriptions, or cues, is heterogeneous and can vary along several dimensions (e.

Rate of cross-commodity discounting of substances varies by substance type for individuals in recovery from substance use disorders.

The extant literature hints at the existence of substance-specific differences in rates of cross-commodity discounting (CCT). However, direct examinations are currently lacking. The present experiment aimed to replicate previous studies examining CCT of substances and to extend their findings by examining potential substance-specific relationships with discounting.

Receptor-based bioassay for functional IL-2 quantification.

IL-2 is a potent cytokine that promotes multiple immune cells proliferation and activation. Accordingly, IL-2 based immunotherapies are emerging to treat cancers or AIDS by enhancing T cell growth and function. Besides, IL-2 is indispensable in in vitro cultivation of immune cells which is a critical step of CAR-T or CAR-NK immunotherapies.

Clinical characteristics of patients with hepatitis and cirrhosis and the construction of a prediction model.

Background: Hepatitis B-associated cirrhosis is an important disease burden in China. However, there is a lack of effective predictors in clinical practice to drive delivery and enable early treatment to delay disease progression.

Aim: To analyzing the clinical characteristics of patients with hepatitis and cirrhosis, the nomogram model was established and validated.

Analysis of associated malformations by computed tomography in adults with polysplenia syndrome: A pilot study.

Objective: To analytically depict the associated malformations of polysplenia syndrome (PS) in adults via computed tomography (CT).

Materials And Methods: The incidence of malformations associated with PS in twelve adult patients was retrospectively analyzed via CT imaging.

Results: The number of splenic nodules ranged from three to twelve; the splenic nodules were located in the left upper quadrant in nine patients and in the right upper quadrant in three patients.

Evolutionary trend analysis of the pharmaceutical management research field from the perspective of mapping the knowledge domain.

Background: Pharmaceutical management is a new frontier subject between pharmacy, law and management, and related research involves the whole process of drug development, production, circulation and use. With the development of medical systems and the diversification of patients' drug needs, research in the field of pharmaceutical management is becoming increasingly abundant. To clarify the development status of this field, this study conducted a bibliometric analysis of relevant literature in the field based on the knowledge graph method for the first time and explored the evolutionary trends of research hotspots and frontiers.

Wu Wei in Mindfulness: A Systematic Perspective.

Resuming the theoretical and historical conceptualization of mindfulness research is of utmost importance if we want to transcend the positivistic and instrumental trend in the present field and to understand better what mindfulness is and how mindfulness works. Based on von Fircks (2023)'s introduction of Daosim's wu wei into Meadian Social Psychology, this commentary continues dialoguing the two different traditions for understanding mindfulness's functioning by exploring the systematic principle underlying wu wei. From the systematic perspective, we can distinguish: 1) two focus of meaning in wu wei: (a) not forcing/interfering and (b) the existence of a spontaneously evolving system; 2) two positions a person can take faced with the spontaneous system: (a) as stepping back and not interfering; (b) as actively connecting and cultivating new relations into systems.

Relationship between dynamic changes of microorganisms in Qupi and the quality formation of Fengxiangxing Huairang Daqu.

Introduction: Fengxiangxing Huairang Daqu (FHD) is one of the major types of Daqu in China. However, the relationship between the microbial community structure at different stages, the changes in the sensory characteristics, fermentation characteristics, volatiles, the most critical process point, and the quality formation of FHD is not clear.

Methods: Based on microscopic characterization, PacBio SMRT sequencing, and HS-SPME-GC-MS volatile metabolite analysis revealed the relationship between FHD quality formation and the dynamics of Qupi.

Mesenchymal stem cells and their extracellular vesicles in bone and joint diseases: targeting the NLRP3 inflammasome.

The nucleotide-binding oligomerization domain-like-receptor family pyrin domain-containing 3 (NLRP3) inflammasome is a cytosolic multi-subunit protein complex, and recent studies have demonstrated the vital role of the NLRP3 inflammasome in the pathological and physiological conditions, which cleaves gasdermin D to induce inflammatory cell death called pyroptosis and mediates the release of interleukin-1 beta and interleukin-18 in response to microbial infection or cellular injury. Over-activation of the NLRP3 inflammasome is associated with the pathogenesis of many disorders affecting bone and joints, including gouty arthritis, osteoarthritis, rheumatoid arthritis, osteoporosis, and periodontitis. Moreover, mesenchymal stem cells (MSCs) have been discovered to facilitate the inhibition of NLRP3 and maybe ideal for treating bone and joint diseases.

Active immunization with recombinant GnRH6-CRM197 inhibits reproductive function of male rats.

Gonadotropin-releasing hormone (GnRH) vaccines have been successfully used for the inhibition of gonadal development and function, but current GnRH-based vaccines often present variability in the response. Cross-reactive material 197 (CRM197) has been used as carrier molecules to enhance an immune response to associated antigens. So, the synthetic mammalian tandem-repeated GnRH hexamer (GnRH6) gene was integrated into the expression plasmid pET-21a.

Regulation of mesenchymal stem cell differentiation by autophagy.

Autophagy, a process that isolates intracellular components and fuses them with lysosomes for degradation, plays an important cytoprotective role by eliminating harmful intracellular substances and maintaining cellular homeostasis. Mesenchymal stem cells (MSCs) are multipotent progenitor cells with the capacity for self-renewal that can give rise to a subset of tissues and therefore have potential in regenerative medicine. However, a variety of variables influence the biological activity of MSCs following their proliferation and transplantation .

Integrative proteomic and transcriptomic analysis in the female goat ovary to explore the onset of puberty.

Puberty is considered a prerequisite for affecting reproductive performance and productivity. Little was known about molecular changes in pubertal goat ovaries. Therefore, we measured and performed a correlation analysis of the mRNA and proteins changes in the pre-pubertal and pubertal goat ovaries.

Grid1 regulates the onset of puberty in female rats.

The study aimed to investigate the effect of Grid1, encoding the glutamate ionotropic receptor delta type subunit 1 (GluD1), on puberty onset in female rats. Grid1 mRNA and protein expression was detected in the hypothalamus of female rats at prepuberty and puberty. The levels of Grid1 mRNA in the hypothalamus, the fluorescence intensity in the arcuate nucleus and paraventricular nucleus of the prepubertal rats was significantly lower than pubertal.

The application of mesenchymal stem cells in the treatment of traumatic brain injury: Mechanisms, results, and problems.

Mesenchymal stem cells (MSCs) are multipotent stromal cells that can be derived from a wide variety of human tissues and organs. They can differentiate into a variety of cell types, including osteoblasts, adipocytes, and chondrocytes, and thus show great potential in regenerative medicine. Traumatic brain injury (TBI) is an organic injury to brain tissue with a high rate of disability and death caused by an external impact or concussive force acting directly or indirectly on the head.

Base-Assisted Polymerizations of Elemental Sulfur and Alkynones for Temperature-Controlled Synthesis of Polythiophenes or Poly(1,4-dithiin)s.

With the increasing demand for functional polythiophenes in extensive applications such as organic solar cells, electronic skins, thermoelectric materials, and field effect transistors, efficient and economic synthetic approaches for polythiophenes are urgently required. In this work, KOH-assisted polymerizations of elemental sulfur and alkynones were developed to directly afford polythiophenes with various backbones, regioselective structures, and high molecular weights (s up to 20700 g/mol) in high yields (up to 97%) at 80 °C in 30 min. When the same polymerization was conducted at room temperature, stable and unique poly(1,4-dithiin)s (s up to 21800 g/mol) could be rapidly obtained in high yields (up to 87%) in 10 min.

Integrative proteomic and phosphoproteomic analysis in the female goat hypothalamus to study the onset of puberty.

Background: Puberty marks the end of childhood and achieve sexual maturation and fertility. The role of hypothalamic proteins in regulating puberty onset is unclear. We performed a comprehensive differential proteomics and phosphoproteomics analysis in prepubertal and pubertal goats to determine the roles of hypothalamic proteins and phosphoproteins during the onset of puberty.

BG2: Bayesian variable selection in generalized linear mixed models with nonlocal priors for non-Gaussian GWAS data.

Background: Genome-wide association studies (GWASes) aim to identify single nucleotide polymorphisms (SNPs) associated with a given phenotype. A common approach for the analysis of GWAS is single marker analysis (SMA) based on linear mixed models (LMMs). However, LMM-based SMA usually yields a large number of false discoveries and cannot be directly applied to non-Gaussian phenotypes such as count data.

Evaluation on coupling coordinated development of population economy and eco-geological environment in the twin-city economic circle of Chengdu-Chongqing region.

Using the CRITIC method, comprehensive index evaluation method, VAR model, coupling coordination model and other methods, this paper evaluates the comprehensive development of the population economy and eco-geological environment composite system in the twin-city economic circle of Chengdu-Chongqing region from 2000 to 2020, verifies the dynamic coupling relationship between subsystems and measures the coupling coordination degree of complex system. Meanwhile, the differences in the development process of each subsystem are discussed and the spatial-temporal evolution characteristics and laws of coupling coordination of the composite system during the research period are analyzed. The results reveal the following: (1) There is a long-term coordinated relationship between population, economy and eco-geological environment in Chengdu-Chongqing region, which have the conditions for emergence and generation.

Bayesian model selection for generalized linear mixed models.

We propose a Bayesian model selection approach for generalized linear mixed models (GLMMs). We consider covariance structures for the random effects that are widely used in areas such as longitudinal studies, genome-wide association studies, and spatial statistics. Since the random effects cannot be integrated out of GLMMs analytically, we approximate the integrated likelihood function using a pseudo-likelihood approach.

A facile bioorthogonal chemistry-based reversible to irreversible strategy to surmount the dilemma between injectability and stability of hyaluronic acid hydrogels.

Injectable and stable hydrogels have great promise for clinical applications. Fine-tuning the injectability and the stability of the hydrogels at different stages has been challenging due to the limited number of coupling reactions. A distinct "reversible to irreversible" concept using a thiazolidine-based bioorthogonal reaction between 1,2-aminothiols and aldehydes in physiological conditions to surmount the dilemma between injectability and stability is presented for the first time.