Integration of SuFEx and Sonogashira Cross-Coupling for the Synthesis of Structurally Diverse Aryl Acetylenes on DNA.

To address the current limitations of DNA-compatible Sonogashira cross-coupling reactions capable of accommodating a broad range of commercially available phenolic building blocks (BBs), an SuFEx-Sonogashira cross-coupling protocol has been developed. This protocol involves the conversion of readily accessible phenolic compounds into the corresponding aryl fluorosulfates within 96-well microplates via a highly efficient liquid-phase SuFEx reaction, followed by Sonogashira cross-coupling with DNA-conjugated terminal alkynes.

Hydrogen Bond Disruption-Induced Ion Rearrangement in Acetonitrile-Water-Sodium Sulfate Solutions.

Understanding hydrogen bonding and ion-specific interactions in water, sodium sulfate (NaSO), and acetonitrile (ACN) systems remains challenging due to their complex, dynamic nature. Here, Raman spectroscopy is employed to probe hydrogen bonding networks and ion reorganization in NaSO aqueous solutions with different ACN concentrations. The results indicate that, at low ACN concentrations in the ternary solutions, hydrogen bonding between ACN and water molecules disrupts the original hydration structure of the ions, resulting in the formation of small ion clusters via electrostatic interactions.

Intestinal epithelial Dicer1 regulates gut microbiome and Alzheimer's pathology in App-knock-in mice.

Background: Alzheimer's disease (AD) patients and animal models exhibit an altered gut microbiome that is associated with pathological changes in the brain. Intestinal miRNA enters bacteria and regulates bacterial metabolism and proliferation. This study aimed to investigate whether the manipulation of miRNA could alter the gut microbiome and AD pathologies.

Dietary inflammatory index and the risk of colorectal adenomas and cancer: a systematic review and dose-response meta-analysis.

Background: The potential association between dietary inflammatory index (DII) and colorectal cancer (CRC) risk, as well as colorectal adenomas (CRA) risk, has been extensively studied, but the findings remain inconclusive. We conducted this systematic review and dose-response meta-analysis to investigate the relationship between the DII and CRC and CRA.

Methods: We comprehensively searched the PubMed, Embase, Cochrane Library, and Web of Science databases for cohort and case-control studies reporting the relationship between DII and CRA, or between DII and CRC, as of 15 July 2025.

Development and validation of a gastric cancer prognostic model utilizing lymphatic endothelial cell-related genes.

Background: Gastric cancer is one of the most common cancers worldwide, with its prognosis influenced by factors such as tumor clinical stage, histological type, and the patient's overall health. Recent studies highlight the critical role of lymphatic endothelial cells (LECs) in the tumor microenvironment. Perturbations in LEC function in gastric cancer, marked by aberrant activation or damage, disrupt lymphatic fluid dynamics and impede immune cell infiltration, thereby modulating tumor progression and patient prognosis.

Patterns of extreme outlier gene expression suggest an edge of chaos effect in transcriptomic networks.

Background: Most RNA-seq datasets harbor genes with extreme expression levels in some samples. Such extreme outliers are usually treated as technical errors and are removed from the data before further statistical analysis. Here we focus on the patterns of such outlier gene expression to investigate whether they provide insights into the underlying biology.

The influence of subjective social class on employment confidence: the chain mediating effect of perceived social support and self-efficacy.

With the persistence of difficult employment, a large number of college students feel anxious and nervous about job hunting. College students with different family economic status have various feelings and performances when faced with employment, possibly due to subjective social class differences. The present study investigated the employment confidence of 611 undergraduates in Chongqing, aimed to ascertain the overall employment confidence of Chinese college students, and tried to analyze how subjective social class works on the employment confidence of college students and its influencing mechanism.

Distinct evolutionary trajectories of subgenomic centromeres in polyploid wheat.

Background: Centromeres are crucial for precise chromosome segregation and maintaining genome stability during cell division. However, their evolutionary dynamics, particularly in polyploid organisms with complex genomic architectures, remain largely enigmatic. Allopolyploid wheat, with its well-defined hierarchical ploidy series and recent polyploidization history, serves as an excellent model to explore centromere evolution.

Barriers and facilitators of the implementation for the antimicrobial Social Norm Feedback (SNF) intervention strategy in primary care institutions: a qualitative study based on the Consolidated Framework for Implementation Research (CFIR).

Background: Although current evidence supports the effectiveness of social norm feedback (SNF) interventions, their sustained integration into primary care remains limited. Drawing on the elements of the antimicrobial SNF intervention strategy identified through the Delphi-based evidence applicability evaluation, this study aims to explore the barriers and facilitators to its implementation in primary care institutions, thereby informing future optimization.

Methods: Based on the five domains of the Consolidated Framework for Implementation Research (CFIR), we developed semi-structured interview and focus group discussion guides.

Clinical Utility of Transcatheter PFO Exploration in Cryptogenic Stroke Patients With Negative TEE but High Suspicion of PFO-Related Etiology.

Background: Patent foramen ovale (PFO) has been identified as a potential risk factor for cryptogenic stroke (CS). Although transesophageal echocardiography (TEE) is considered the gold standard for PFO detection, false-negative results remain a clinical concern, particularly in CS patients with high suspicion of PFO-related etiology.

Aims: To evaluate the clinical utility of transcatheter PFO exploration (TPFOE) in CS patients with negative TEE findings but high suspicion of PFO-related etiology.

Construction of a nomogram for hypertriglyceridemic severe acute pancreatitis that includes metabolic indexes.

Background: Current scoring systems for hypertriglyceridaemia-induced acute pancreatitis (HTG-AP) severity are few and lack reliability. The present work focused on screening predicting factors for HTG-SAP, then constructing and validating the visualization model of HTG-AP severity by combining relevant metabolic indexes.

Methods: Between January 2020 and December 2024, retrospective clinical information for HTG-AP inpatients from Weifang People's Hospital was examined.

U-shaped association of body roundness index with all-cause and cardiovascular mortality in individuals with chronic kidney disease.

Purpose: This study aimed to investigate the association between body roundness index (BRI) and deaths from all causes and cardiovascular disease (CVD) in participants with chronic kidney disease (CKD).

Materials And Methods: The data was sourced from the National Health and Nutrition Examination Survey (NHANES) 1999-2018. Cox proportional hazards regression along with restricted cubic splines were applied to assess the associations of BRI with deaths from all causes and CVD in individuals with CKD.

Epidemiology, resistance profiles, and risk factors of multidrug- and carbapenem-resistant Serratia marcescens infections: a retrospective study of 242 cases.

Background: Serratia marcescens is an opportunistic pathogen increasingly associated with healthcare-associated infections and rising antimicrobial resistance. The emergence of multidrug-resistant (MDR) and carbapenem-resistant S. marcescens (CRSM) presents significant therapeutic challenges.

Sex-specific association of novel complex inflammatory markers with colorectal polyps: mediating effects of triglyceride-glucose and non-high-density lipoprotein cholesterol.

Background: The CRP-albumin-lymphocyte (CALLY) index has potential clinical value as a novel marker integrating inflammatory, nutritional and immune status in the development of colorectal polyps. This study examined whether gender factors influence the association between CALLY and colorectal polyps; in addition to elucidating whether metabolic pathways mediate this relationship.

Methods: This is a cross-sectional study including 5409 adult health screening participants who completed colonoscopy.

Red blood cell transfusion frequency and the risk and severity of bronchopulmonary dysplasia in preterm infants: a retrospective cohort study.

Background: Red blood cell (RBC) transfusion is a common intervention for anemia in preterm infants; however, its association with bronchopulmonary dysplasia (BPD) remains debated. While biological mechanisms suggest potential harm, the clinical impact of transfusion frequency on BPD incidence and severity remains unclear.

Objective: To investigate whether RBC transfusion frequency is independently associated with the risk and severity of BPD in preterm infants born before 32 weeks of gestation.

Latent class analysis of depression among maintenance hemodialysis patients in China: a multicenter cross-sectional study.

Background: Depression is a common mental disorder in hemodialysis patients. The present study aimed to identify subgroups of patients receiving hemodialysis based on depression and explore the influencing factors in a multicenter hemodialysis population in China.

Methods: A total of 1,090 hemodialysis patients (682 men, mean aged 61.

Predicting kidney replacement therapy, cardiovascular disease and all-cause mortality in advanced chronic kidney disease among the Chinese population.

The Grams model, designed to predict adverse event risks in advanced chronic kidney disease (CKD) patients, was evaluated in a Chinese cohort of 1,333 patients with eGFR below 30 mL/min/1.73 m. The model demonstrated moderate to good discrimination across outcomes, performing well in predicting kidney replacement therapy (KRT) but overestimating the risks of cardiovascular disease (CVD) and mortality.

Clinical characteristics of IgG4-related lung disease: a single-center based experience.

Background: IgG4-related lung disease (IgG4-RLD) is a rare autoimmune condition. This study aims to systematically analyze the clinical characteristics of IgG4-RLD to enhance clinicians' awareness and improve patient outcomes.

Methods: This retrospective analysis investigates the clinical data of 20 patients diagnosed with IgG4-RLD at the Yichang Central People's Hospital between January 2019 and April 2025.

Myogenic induced adipose-derived stem cells sheets combined with electrospun scaffolds of silk fibroin and poly(lactide-co-glycolide) for stress urinary incontinence treatment.

The stress urinary incontinence (SUI) is a difficulty in urology and current sub-urethral sling treatments are associated with inflamation and recurrence. In this study, we developed a novel tissue-engineered sling with myogenic induced adiposederived stem cells (MI-ADSCs) sheets induced by 5-Aza and combined with electrospun scaffolds of silk fibroin and poly(lactide-co-glycolide) (SF/PLGA) for the treatment of stress urinary incontinence. MI-ADSCs increased α-SMA, MyoD and Desmin the mRNA and protein expression.

Transcriptional condensates enrich phosphorylated PRMT2 to stimulate H3R8me2a deposition and hypoxic response in glioblastoma.

Histone arginine methylation by protein arginine methyltransferases (PRMTs) is crucial for transcriptional regulation and is implicated in cancers. Despite their therapeutic potential, some PRMTs present challenges as drug targets due to their context-dependent activities. Here, we demonstrate that hypoxia triggers the rapid condensation of PRMT2, which is essential for its histone H3R8 asymmetric dimethylation (H3R8me2a) activity.

Temporal transcriptomics reveal crucial networks underlying jasmonate-mediated diurnal floret opening and closure in rice.

Diurnal floret opening and closure (DFOC) is essential for rice reproductive development and hybrid breeding, yet transcriptional dynamics and underlying regulatory networks remain poorly characterized. Here, we conducted high-temporal-resolution transcriptomic analyses of lodicules to dissect DFOC regulatory networks in two japonica rice cultivars. Analysis of differentially expressed genes (DEGs) uncovered core genes shared by both cultivars, primarily associated with jasmonic acid (JA) signaling and cell wall remodeling.

Engineering resistance genes against tomato brown rugose fruit virus.

Tomato brown rugose fruit virus (ToBRFV) overcomes all known tomato resistance genes, including the durable Tm-2, posing a serious threat to global tomato production. Here, we employed in vitro random mutagenesis to evolve the Tm-2 leucine-rich repeat (LRR) domain and screened ∼8,000 variants for gain-of-function mutants capable of recognizing the ToBRFV movement protein (MP) and triggering hypersensitive cell death. We identified five such mutants.