Application of engineered CRISPR/Cas12a variants with altered protospacer adjacent motif specificities for the detection of isoniazid resistance mutations in .

Unlabelled: Drug-resistant tuberculosis (TB) is a major global public health concern. Although isoniazid is currently considered one of the most effective first-line drugs for TB treatment, its efficacy is limited by the emergence of resistance. Therefore, it is imperative to develop new methods for detecting drug-resistant TB.

Diagnostic accuracy of nanopore sequencing for the rapid diagnosis of extrapulmonary tuberculosis: a protocol for a systematic review and meta-analysis.

Background: Extrapulmonary tuberculosis (EPTB) is a serious type of tuberculosis (TB) which can cause systemic clinical manifestations. Rapid diagnosis of EPTB for intervention is of great importance. Nanopore sequencing as a third-generation gene sequencing method is a new kind of rapid TB detection.

Clinical and epidemiological analysis of 893 patients with spinal tuberculosis: an 11-Year investigation of a general hospital in East China.

Background: The burden of spinal tuberculosis (STB) in China remains substantial, with the country ranking third in the number of tuberculosis cases globally in 2022, among the 30 countries with a high tuberculosis burden. In East China, few large-scale studies have been conducted on STB.

Methods: This retrospective study analyzed 893 confirmed STB cases (2010-2020).

An Interpretable Radiomics Model Integrating Ultrasound and Clinical Features for Multiclass Classification of Axillary Lymph Nodes.

Objective: Accurate identification of common lesions (benign, metastatic carcinoma, lymphoma) of axillary lymph nodes (ALNs) is of vital importance for clinical decision-making, directly affecting tumor staging, treatment strategy formulation and prognosis of patients. However, current diagnostic methods by radiologists have limited accuracy in differentiating between benign lesions, metastatic carcinoma, and lymphoma.This study aimed to develop and validate the Axillary Lymph Node Interpretable Prediction (ALNIP) model for classifying axillary lymph nodes into benign, metastatic carcinoma, or lymphoma based on ultrasound imaging.

Testicular immunosenescence: a key player in age-related spermatogenic decline.

As global populations age, testicular aging has become a key contributor to the gradual decline in male fertility, characterized by lower sperm count, poorer sperm quality, and reduced reproductive potential. While the testis is traditionally viewed as an immune-privileged site, growing evidence shows that this immune protection weakens over time-a process now known as testicular immunosenescence. This review provides a comprehensive overview of age-related changes in the testicular immune landscape.

Utility of novel composite index in predicting the prognosis of total neoadjuvant therapy for locally advanced rectal cancer: A multicenter real-world cohort study.

Objective: C-reactive protein-albumin-lymphocyte (CALLY) is a novel composite index. Total neoadjuvant therapy (TNT) has become one of the standard treatments for locally advanced rectal cancer (LARC). It remains unclear whether CALLY can predict the prognosis for LARC patients.

A case of rare fungal keratitis caused by Pseudoshiraia conidialis.

Rationale: Pseudoshiraia conidialis is a filamentous fungus that belongs to the Shiraiaceae family. We report a rare case of fungal keratitis caused by P conidialis.

Patient Concerns: A 61-year-old female accidentally scratched her right eye with a bamboo branch, presenting with conjunctival congestion in the right eye, corneal ulcer with edema endothelial folds, anterior chamber exudation hypopyon, and lens opacity.

Analysis of bleeding after ultrasound-guided core needle biopsy of infected cervical lymph nodes.

Purpose: This study aimed to determine the incidence of bleeding after core needle biopsy (CNB) of infected cervical lymph nodes and analyze the factors associated with bleeding after CNB, and preventive measures of bleeding.

Methods: We retrospectively analyzed the records of 643 patients with infectious cervical lymphatic ganglionic diseases who underwent CNB at our hospital from December 2015 to February 2022. The number of patients with bleeding after CNB, extent of bleeding, and type of disease were recorded and statistically analyzed.

[Electroacupuncture alleviates neuropathic pain hyperalgesia and anxiety-like behaviors via the dorsal raphe nucleus-basolateral amygdala circuit].

Objectives: This study aims to investigate the intervention effects of electroacupuncture on neuropathic pain and anxiety-like behavior, and to explore the underlying mechanisms of the DRN-BLA (dorsal raphe nucleus serotoninergic - basolateral amygdala) neural circuit.

Methods: Male C57BL/6J mice were selected, and a neuropathic pain model was established through spared nerve injury (SNI) surgery. Electroacupuncture (100 Hz, 0.

Acute lymphoblastic leukemia with infection: a case report.

Background: infections are rare but life-threatening in immunocompromised patients, particularly those with acute lymphoblastic leukemia (ALL) undergoing chemotherapy. This case report describes a patient with ALL who developed multifocal cutaneous infections following VDCLP chemotherapy, highlighting the challenges in diagnosis and management.

Case Presentation: A 27-year-old male ALL patient developed cutaneous infections on the right calf, left upper arm, and buttock during chemotherapy.

A scoring model based on MRI features for predicting early recurrence after surgical resection of hepatocellular carcinoma.

Objectives: Based on MRI features, a scoring model was constructed to predict early recurrence after surgical resection of hepatocellular carcinoma (HCC).

Methods: A total of 310 patients from two centers with HCC (212 in the training cohort, 98 in the validation cohort) were collected from January 2017 to October 2023, all patients underwent preoperative MRI-enhanced examinations and were pathologically diagnosed after resection and were divided into early recurrence group and non-early recurrence group based on follow-up results. Clinical, laboratory, and MRI features of patients were collected and subjected to statistical analysis.

Preclinical evidence for luteolin in ulcerative colitis: a meta-analysis and systematic review.

Background: Evidence suggests that luteolin (LUT) may offer therapeutic potential in treating ulcerative colitis (UC), though its specific pharmacological mechanisms remain incompletely understood. This meta-analysis aims to assess the pharmacological effects of LUT in UC animal models and investigate its potential mechanisms of action.

Methods: A comprehensive search of five databases, namely, PubMed, Web of Science, Embase, EBSCO, and ScienceDirect, was conducted to identify studies investigating the effects of LUT on UC.

Clinical utility of ultrasound radiomics models in differentiating cervical tuberculous lymphadenitis from cervical lymph node metastasis.

ObjectiveCervical tuberculous lymphadenitis (CTBL) and cervical lymph node metastasis (CLNM) share similar imaging characteristics, making differentiation challenging. This study aims to evaluate the clinical utility of a multimodal radiomics model combining grayscale ultrasound (GUS), elastography ultrasound (EUS), and contrast-enhanced ultrasound (CEUS) for distinguishing CTBL from CLNM.MethodsA high-quality dataset comprising 203 cases of CTBL was used to train and test the radiomics models.

Assessment of Pharmacokinetics and Safety with Bioequivalence of the Nitroglycerin Sublingual Tablets of Two Formulations in Chinese Healthy Subjects: A Bioequivalence Study.

Background And Objective: Nitroglycerin, a cornerstone therapy for acute angina pectoris, achieves rapid symptom relief through sublingual administration by bypassing hepatic first-pass metabolism. This study aimed to investigate the pharmacokinetics (PK), bioequivalence, and safety profiles between a test (T) formulation and a reference (R) formulation of nitroglycerin sublingual tablets in healthy volunteers (HVs).

Methods: In this single-center, randomized, open, single-dose, two-part formulations, four-cycle, two-sequence complete repeat crossover design, fasting-dose bioequivalence study, HVs (n = 36) were 1:1 divided into two groups (T-R-T-R and R-T-R-T) and received 0.

Case Report: Carotid cavernous fistula presenting as red eye: case illustration and comprehensive review.

Carotid cavernous fistula (CCF) is a pathological condition where an abnormal connection forms between the carotid artery and the cavernous sinus. This condition can lead to a variety of ocular and neurological symptoms, often making it difficult to diagnose, especially when patients present with common ocular complaints such as red eye, which is often misdiagnosed as conjunctivitis. The subtle and diverse symptoms of CCF can lead to misdiagnosis, delaying appropriate treatment.

Diagnostic Utility of Nanopore Sequencing for Tuberculous Serous Effusions.

Objective: Early and precise diagnosis of tuberculous serous effusions is a huge challenge. Nanopore sequencing is a potentially efficient assay. The objective of the current study was to evaluate the diagnostic accuracy of nanopore sequencing for tuberculous serous effusions using clinical specimens directly, and to provide a new pathway for the early and precise diagnosis of tuberculous serous effusions.

Non-targeted metabolomics and machine learning reveal metabolic dysregulation in lymph node tuberculosis.

Background: Lymph node tuberculosis (LNTB) is the most prevalent form of extrapulmonary tuberculosis; however, differentiating it from non-LNTB remains challenging due to overlapping clinical features and suboptimal diagnostic methods. Current diagnostic methods for LNTB lack both sensitivity and specificity. This study aimed to characterize the metabolic differences between LNTB and non-LNTB patients, elucidate the pathological mechanisms underlying LNTB, and identify diagnostic biomarkers using machine learning models.

A Novel Nomogram to Differentiate Between Renal Tuberculosis and Nontuberculous Renal Infection.

Background: To build a diagnostic nomogram for differentiating between renal tuberculosis (RTB) and nontuberculous renal infection.

Methods: Eligible patients were randomly categorized into derivation and validation cohorts (7:3). Univariate and multivariate regression analyses were conducted to filter variables and select predictors.

Protects Against Steroid-Induced Osteonecrosis of the Femoral Head Through Inhibiting Inflammatory Responses and Apoptosis via Network Pharmacology and Experimental Validation.

Background: Steroid-related osteonecrosis of the femoral head (SONFH) is bone death resulting from the use of chronic glucocorticoids. Due to its high incidence and lack of effective treatment, which is still a challenging problem in orthopedic surgery. Cornus officinalis (SZY) is usually used as a traditional use for the treatment of SONFH and plays a major role in traditional prescriptions, however, the specific pharmacological mechanisms of action remain unclear.

Finger drawing on smartphone screens enables early Parkinson's disease detection through hybrid 1D-CNN and BiGRU deep learning architecture.

Background: Parkinson's disease (PD), a progressive neurodegenerative disorder prevalent in aging populations, manifests clinically through characteristic motor impairments including bradykinesia, rigidity, and resting tremor. Early detection and timely intervention may delay disease progression. Spiral drawing tasks have been established as effective auxiliary diagnostic tools.

Inflammatory Biomarker-Based Risk Prediction Model for Endovascular Reconstruction in Acute Lower Limb Ischemia: A Multicenter Development and Validation Study.

Background: Inflammatory markers are associated with poor prognosis of peripheral vascular diseases. We aim to determine the relationship between inflammatory markers and the prognosis of acute lower limb ischemic disease and to construct and verify a prognostic model based on inflammatory indicators.

Methods: We evaluated 295 patients with a diagnosis of acute lower limb ischemia (ALI) from multiple centers between 2020 and 2023.