Transcriptional regulation in heart development, disease and regeneration: reassessing the fetal gene hypothesis.

A central paradigm in cardiac biology is the reactivation of the fetal gene programme in the adult heart in response to stress. This so-called 'fetal gene hypothesis' was first proposed almost 40 years ago following the observation that certain fetal contractile protein isoforms were re-expressed in hypertrophied ventricles in the rodent heart in response to haemodynamic overload. Consequently, this concept was broadly adopted, and activation of the fetal gene programme became synonymous in the literature with the cardiac stress response.

Decreasing H3K27me3 Alleviates Cerebral Ischemia/reperfusion Injury by Modulating FOXP1 Expression.

Elevated H3K27me3 levels during cerebral I/R injury exacerbate neuronal damage through oxidative stress, but the underlying mechanism remains to be elucidated. We hypothesized that reduced H3K27me3 confers protection by modulating FOXP1 expression. Employing multifaceted approaches, we demonstrate that H3K27me3 reduction in vivo and in vitro enhances lipid metabolism and rescues oxygen-glucose deprivation (OGD)-induced mitochondrial morphological abnormalities and functional deficits.

Elevated level of platelet factor 4 in follicular fluid is associated with polycystic ovary syndrome.

Objective: This present study aimed to measure platelet factor 4 (PF4) protein level in follicular fluid of patients with polycystic ovary syndrome (PCOS) and analyzed the correlation between follicular PF4 level with clinical characteristics.

Methods: Sixty-seven women (36 PCOS patients vs. 31 non-PCOS women) were enrolled in the study.

Inhibition of Mycobacterium tuberculosis UvrB by small molecules: Potent NER disruption and structural insights into dimer conformation.

The nucleotide excision repair (NER) pathway in Mycobacterium tuberculosis (Mtb) is important for DNA damage repair and bacterial survival under stress, yet specific inhibitors targeting its components remain scarce. Here, we targeted the UvrB protein, a central component of the Mtb UvrABC NER pathway, and identified novel small molecule inhibitors against its nucleotide binding domain (NBD). Using in silico structure-based screening involving the Maybridge library (~54,000 compounds), Molecular dynamics (MD) simulations, and Biolayer interferometry (BLI), we identified four potent inhibitors: SPB08143, RJC04069, NRB00936, and DP00786 with IC50 values of 9.

Effect of Oral Direct Factor Xa Inhibitors on the Prevalence of Hip Heterotopic Ossification following Arthroplasty for Acute Femoral Neck Fracture: A Propensity Score-Matched Cohort Study.

Background: Heterotopic ossification (HO) is a common complication following hip arthroplasty that can limit hip range of motion (ROM). Oral direct factor Xa (FXa) inhibitors are commonly used anticoagulants after arthroplasty; however, they have a high risk of local bleeding and hematoma formation, which are significant triggers for HO formation. To our knowledge, there is no evidence regarding whether FXa inhibitors will increase HO incidence following hip arthroplasty.

An improving spectral PTF for mining area soil water content prediction: combining 2D correlation spectroscopy and soil-crop indicators with ResGRU.

Conventional methods for soil sampling and soil water content (SWC) measurement are often labor-intensive and time-consuming. The Pedo-transfer function (PTF) integrating soil spectroscopy with soil physicochemical properties provides a more efficient approach for SWC estimation. However, existing studies highlight regional limitations in the accuracy of PTFs across diverse geographical regions.

Noninvasive assessment of glymphatic system alterations as potential biomarkers for predicting overall survival in glioma.

Objective: In this retrospective study, authors aimed to evaluate the glymphatic function alterations associated with glioma and explore the prognostic value of these alterations by calculating the index for diffusivity along the perivascular space (ALPS index).

Methods: The authors utilized data from the publicly available University of California San Francisco Preoperative Diffuse Glioma MRI (UCSF-PDGM) dataset, which includes 501 adult patients with histopathologically confirmed diffuse glioma, per the 2021 WHO classification, who underwent preoperative MRI, initial tumor resection, and tumor genetic testing at a single medical center from 2015 to 2021.The ALPS index was calculated from diffusivity maps for noninvasive glymphatic system (GS) analysis.

Cooperative contribution of multiple energy substrate pathways to floral thermogenesis in sacred lotus.

Floral thermogenesis in lotus (Nelumbo nucifera) is a highly energy-intensive process, requiring substantial metabolic reconfiguration and substrate input. However, the mechanisms coordinating energy substrate supply during this process remain unclear. Here, we integrated microscale proteomics, time-series transcriptomics, and mitochondrial feeding assays to elucidate the substrate provisioning strategies supporting thermogenesis in lotus receptacles.

Single Te Nanoribbon for Disrupting Conventional Sensitivity-Power Limits of Flexible Strain Sensors.

Flexible strain sensors are pivotal for the advancement of robotics, wearable healthcare, and human-machine interaction in the post-Moore era. However, conventional materials struggle to simultaneously achieve high sensitivity, a broad strain range, and low power consumption for cutting-edge applications. In this work, the issue is addressed through single crystal 1D tellurium nanoribbons (NRs), which are synthesized on SiO/Si substrate by hydrogen-assisted chemical vapor deposition (CVD) method.

Visible-Light-Driven TiO/Pt Nanozyme-Embedded Dressings: Integrating PDT, Oxygenation, and Biomechanical Support for Pressure Ulcer Repair.

Pressure ulcer (PU) cause metabolic disorders and ischemia via prolonged pressure, leading to secondary infection, inflammation, and vascular neuropathy. However, existing therapies rely on microenvironment, HO, low repair efficiency, and lack efficient collaborative therapy. Herein, a confined multifunctional TiO/Pt nanozyme is developed via atomic layer deposition for PUs repair.

Spin-Active Polarons Induced Nucleophilic Substitution on Fluorographene for In Situ Fabricating Distinctively 2D-Based Bifunctional Nanocomposites.

Nucleophilic substitution reaction (SR) plays a crucial role in traditional organic chemistry, and its extension into two dimensional (2D) materials has recently created a series of functional materials and advanced applications. However, the traditional SR process is usually indiscriminately copied into existing 2D reaction systems, which tremendously restricts the development of special 2D functional materials and sophisticated applications. In this work, a brand-new spin-active polarons-induced SR process in typical 2D fluorographene (FG) is proposed, where nucleophiles preferentially attack particular oxygen groups for functional grafting, simultaneously inducing the generation of spin-active polarons and subsequently rapid defluorination on the FG nanosheet.

Cutting-edge technologies in neural regeneration.

Neural regeneration stands at the forefront of neuroscience, aiming to repair and restore function to damaged neural tissues, particularly within the central nervous system (CNS), where regenerative capacity is inherently limited. However, recent breakthroughs in biotechnology, especially the revolutions in genetic engineering, materials science, multi-omics, and imaging, have promoted the development of neural regeneration. This review highlights the latest cutting-edge technologies driving progress in the field, including optogenetics, chemogenetics, three-dimensional (3D) culture models, gene editing, single-cell sequencing, and 3D imaging.

Astrocyte Autophagy in Neurodegenerative Diseases: Current Progress in Mechanisms and Therapeutics.

Astrocytes, the most abundant and functionally diverse glial cell type in the brain, play a crucial role in maintaining cellular homeostasis and promoting neuronal survival. Autophagy is the process of transferring senescent, denatured, or damaged proteins and organelles from cells to lysosomes for degradation. However, recent research on autophagy in the central nervous system has focused on neurons.

Design Parametrization of Central Venous Catheters for Pediatric Dialysis: Supporting the Quest for the Most Influential Features.

Pediatric hemodialysis is a life-saving treatment for children with chronic kidney diseases. Central venous catheters (CVCs) are the most commonly used vascular access, despite being commonly subject to complications leading to inadequate hemodialysis and catheter replacement. The available CVCs feature various design elements reflecting ongoing efforts to achieve optimal performance.

A Framework for Integrating Students From Communication Sciences and Disorders Into Simulation-Enhanced Interprofessional Education.

This article provides a framework for simulation educators to integrate students from Communication Sciences and Disorders (CSD) into simulation-enhanced interprofessional education (Sim-IPE). Despite their essential contributions to managing communication, hearing, and swallowing disorders, CSD students remain underrepresented in interprofessional simulations. Drawing on current literature and guided by a 4-step integration framework, the article outlines practical strategies for inclusive scenario design, role clarity, interprofessional reflection, and program refinement.

Metabolomics reveal distinct molecular pathways associated with future risk of Crohn's Disease.

Host - microbiome interactions are central to Crohn'sdisease (CD) pathogenesis; yet the early metabolic alterations that precededisease onset remain poorly defined. To explore preclinical metabolicsignatures of CD, we analyzed baseline serum metabolomic profiles in a nestedcase-control study within the Crohn's and Colitis Canada - Genetics, Environment, Microbiome (CCC-GEM) Project, a prospective cohort of 5,122 healthyfirst-degree relatives (FDRs) of CD patients. We included 78 individuals wholater developed CD and 311 matched FDRs who remained disease-free.

Elevated Soluble HLA-G Levels Associate With Dengue Severity in Vietnamese Patients.

The pathogenesis of dengue remains complex and incompletely understood. One proposed mechanism involves the virus evading host immune responses through the upregulation and/or secretion of immune-inhibitory molecules. This study investigates the association between plasma levels of soluble human leukocyte antigen G (sHLA-G), a known immunoregulatory molecule, and dengue severity in hospitalized patients.

A dual-site doping strategy to enhance the sodium storage performance of an O3-type layered sodium oxide cathode.

The O3-type NaNiFeMnO material is modified by introducing Sr into the Na sites and Al into the transition metal (TM) sites. The inactive Sr and Al serve as structural pillars within the NaO and TMO slabs, respectively, expanding the interlayer spacing and boosting the stability of the structure. The optimized cathode demonstrates a good rate performance of 117.

Precisely Regulating the Graphitic Layers of Hard Carbon via Oxygen Release to Elucidate the Formation Mechanism of Closed Pores.

Hard carbon (HC), recognized as the most promising anode material for sodium-ion batteries (SIBs), exhibits multiple forms of sodium storage (adsorption on graphitic layers, insertion between graphitic sheets, and filling in closed pores). Low initial coulombic efficiency (ICE) and low plateau region capacity are the main issues with HC, and it is necessary to understand the evolution laws of graphitic layers and closed pores. Here, we regulate the structure of graphitic layers by deliberately changing the oxygen content in HC materials and reveal the mechanism of formation of closed pores.

Fluoroalkyl-based near-infrared acceptors for morphology control enabling high-efficiency additive-free organic solar cells.

Herein, a fluoroalkyl side chain modified A-DA'D-A small molecule acceptor, Y18-F9, was developed to optimize the bulk heterojunction morphology in organic solar cells. The introduction of fluorocarbon chains promotes self-assembly into nanoscale fibrous networks, while the low surface energy drive favorable vertical phase segregation. These synergistic effects lead to enhanced molecular packing, improved charge transport and collection, and reduced recombination losses.

[Expression of Concern] S100A8‑targeting siRNA enhances arsenic trioxide‑induced myeloid leukemia cell death by down‑regulating autophagy.

Following the publication of this paper, it was drawn to the Editor's attention by a concerned reader that, for the western blots shown in Fig. 4C, the two left lanes in the 'Fibrillarin' gel slice appeared to be strikingly similar to the mirrored two right lanes in the 'Actin' gel slice, albeit the orientations of the blots were horizontally reversed, such that data which were intended to have shown the results of differently performed experiments appeared to have been derived from the same original source. Moreover, the control β-actin blots featured in Fig.

Network Meta-Analysis of Quality of Life in Heart Failure With Reduced Ejection Fraction.

Background: Although the effects of various combinations of treatments on mortality and morbidity outcomes in heart failure with reduced ejection fraction (HFrEF) have been evaluated, the impact on quality of life is unknown. This study evaluated and compared the composite impact of pharmacological therapies on quality of life in HFrEF using a frequentist network meta-analysis and systematic review methodology.

Methods: We searched MEDLINE, EMBASE, and Cochrane Central Register of Controlled Trials for randomized controlled trials published between January 1, 2021 and August 10, 2024.

Untangling teacher burnout: a network analysis of demands, resources, and out-of-field teaching challenges in rural China.

Introduction: Teacher burnout poses a significant threat to the sustainability of rural education. However, the effect of out-of-field teaching as a job demand remains understudied. This study applies the Job Demands-Resources (JD-R) model to explore how job demands, job resources, and personal resources interact with burnout among rural teachers.

GenoStaR: An R Package for Genotype to Star Allele Conversion for Major Cytochrome P450 Family of Genes.

Pharmacogenomics enables the personalization of drug therapy by linking genetic variations to differences in drug metabolism, efficacy, and risk of adverse reactions. Genetic polymorphisms within cytochrome P450 (CYP) genes significantly affect enzyme activity, influencing drug plasma levels, responses, and safety. Central to this process is accurate genotype-to-phenotype translation, especially for the CYP enzyme family, which metabolizes 70-80% of clinically used drugs.

In-Built Compatible Electrode-Electrolyte Interphases for Quasi-Solid-State Li-SPAN Batteries.

Lithium-sulfur batteries have been regarded as a promising candidate for next-generation energy storage systems owing to their high energy density and low cost. Sulfurized polyacrylonitrile (SPAN) as a cathode material has received wide interest due to the solid-solid conversion mechanism, while the Li-SPAN cell performance has been limited by the notorious issue of lithium metal anode. Developing solid-state electrolytes for lithium-sulfur batteries with favorable electrode-electrolyte compatibility is urgently desired.