Cerium Oxide Nanoparticles Achieve Long-Lasting Senescence Inhibition in an Aging Mouse Model of Sarcopenia via Reactive Oxygen Species Scavenging and CILP2 Downregulation.

Most drugs used to treat sarcopenia are ineffective. Herein, the long-acting anti-sarcopenic properties of cerium oxide nanoparticles (CeNPs) and their underlying mechanisms of action are investigated in aging mice (treated with 4-hydroperoxy cyclophosphamide (4-HC)). CeNPs (size, 27.

The impact of Bifidobacterium breve BBr60 (BBr60) on metabolic and gastrointestinal health in healthy adults: A combined in vitro metabolomic and randomized, double-blind, placebo-controlled study.

Purpose: This study aims to evaluate the physiological and psychological health impacts of Bifidobacterium breve BBr60 (BBr60) on healthy adults.

Methods: The study was divided into preclinical research and a clinical trial phase. In the preclinical study, the bioactive components of BBr60 were analyzed through LC-MS targeted metabolomics.

Comparison of the efficacy and safety of different growth factors in the treatment of diabetic foot ulcers: an updated network meta-analysis.

Objective: This study aimed to evaluate the efficacy and safety of different growth factors (GFs) in the treatment of diabetic foot ulcers (DFUs) through a network meta-analysis.

Methods: A systematic search was conducted in PubMed, Embase, The Cochrane Library, and Web of Science to identify randomized controlled trials (RCTs) comparing GFs with standard of care (SOC) or comparing different GFs for the treatment of DFU. Two independent reviewers screened the studies, extracted data, and assessed the quality of the included literature according to predefined inclusion and exclusion criteria.

The delaying effect of toripalimab on disease progression in patients with advanced hepatocellular carcinoma and changes in serum tumor markers.

Objective: This study aims to retrospectively analyze the delaying effect of toripalimab on disease progression in patients with advanced HCC and to evaluate its impact on serum tumor marker levels.

Methods: In this single-center retrospective study, 80 advanced HCC patients treated between January 2021 and January 2023 were divided into two groups: the intervention group ( = 40) receiving TACE plus toripalimab (240 mg every 3 weeks), and the control group ( = 40) receiving TACE alone. Primary endpoints included objective response rate (ORR) and overall survival (OS); secondary endpoints encompassed tumor marker dynamics, liver function parameters, T lymphocyte subsets, and safety.

The Follicular Output Rate was Improved with 3-Day Letrozole Administration Compared with 5-Day Letrozole Administration Under Progestin-Primed Ovarian Stimulation.

Purpose: Progestin-primed ovarian stimulation (PPOS) has been widely employed in in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles. In recent years, letrozole (LE) combined with medroxyprogesterone acetate (MPA) has been used in this protocol to enhance ovarian response. This study compared the effects of a 5-day regimen with those of a 3-day regimen of letrozole within PPOS, focused on the follicular output rate (FORT) and blastocyst formation rates.

Serum concentrations of medroxyprogesterone acetate were undetectable on OPU+5 days and had no effect on the serum progesterone level in patients undergoing the progestin-primed ovarian stimulation protocol.

Objective: To evaluate the dynamics of serum medroxyprogesterone acetate (MPA) concentrations and their influence on serum progesterone (P) levels and pregnancy outcomes in the progestin-primed ovarian stimulation (PPOS) protocol. A total of 116 patients who underwent fertilization/intracytoplasmic sperm injection (IVF/ICSI) treatment using the PPOS protocol were included. Serum MPA levels were measured on the third, fifth, and seventh days of MPA use; on the day of human chorionic gonadotropin (hCG) trigger; and two and five days after oocyte pick-up (OPU).

AI-assisted warfarin dose optimisation with CURATE.AI for clinical impact: Retrospective data analysis.

Background: Standard-of-care for warfarin dose titration is conventionally based on physician-guided drug dosing. This may lead to frequent deviations from target international normalized ratio (INR) due to inter- and intra-patient variability and may potentially result in adverse events including recurrent thromboembolism and life-threatening hemorrhage.

Objectives: We aim to employ CURATE.

Deacetylation of ANXA2 by SIRT2 desensitizes hepatocellular carcinoma cells to donafenib via promoting protective autophagy.

Hepatocellular carcinoma (HCC) is one of the most lethal forms of cancer globally. HCC cells frequently undergo macroautophagy, also known as autophagy, which can lead to tumor progression and chemotherapy resistance. Annexin A2 (ANXA2) has been identified as a potential therapeutic target in HCC and is involved in the regulation of autophagic process.

Deep-learning-enabled spatial frequency domain imaging of the spatiotemporal dynamics of skin physiology.

Significance: Spatial frequency domain imaging (SFDI) is an emerging optical imaging modality for visualizing tissue absorption and scattering properties. This approach is promising for noninvasive wide field-of-view (FOV) monitoring of biophysiological processes .

Aim: We aim to develop deep-learning-enabled spatial frequency domain imaging (SFDI-net) for real-time large FOV imaging of the optical, structural, and physiological properties and demonstrate its application for probing the spatiotemporal dynamics of skin physiology.

Antinuclear antibodies do not affect euploidy rate in preimplantation genetic testing for aneuploidy.

Research Question: Do antinuclear antibodies affect the blastocyst aneuploidy rate during preimplantation genetic testing for aneuploidy (PGT-A) cycles?

Design: A retrospective cohort study was undertaken of 259 patients who underwent PGT-A and immunological screening at the study centre between May 2018 and May 2023. Patients were divided into an antinuclear-antibody-negative group (213 cycles) and an antinuclear-antibody-positive group (46 cycles) based on the presence/absence of antinuclear antibodies, and embryo outcomes were compared.

Results: Significant differences were found between antinuclear-antibody-negative and antinuclear-antibody-positive patients in terms of paternal age (34 versus 37 years; P = 0.

Nanoengineered Shape-Memory Hemostat.

Uncontrolled hemorrhage is the predominant cause of preventable combat deaths. Various biomaterials serve as hemostatic agents due to their procoagulant or absorptive activity. However, these biomaterials often lack expansion capabilities, which severely limits use in noncompressible wounds.

Polyphenol-Mediated Multifunctional Human-Machine Interface Hydrogel Electrodes in Bioelectronics.

Human-machine interface (HMI) electrodes enable interactions between humans and bioelectronic devices by facilitating electrical stimulation and recording neural activity. However, reconciling the soft, hydrated nature of living human tissues with the rigid, dry properties of synthetic electronic systems is inherently challenging. Overcoming these significant differences, which is critical for developing compatible, effective, and stable interfaces, has become a key research area in materials science and technology.

Tumor Antigen-Coated Two-Dimensional Black Phosphorus as a Nanovaccine for Synergistic Cancer Photothermal Therapy and Immunotherapy.

Vaccine immunotherapy is paving the way for an effective long-term immune response and targeted destruction of tumor cells and shows promise as a leading strategy in tumor treatment. Nanoparticles are crucial in combining vaccine immunotherapy and photothermal therapy (PTT), generating local tumor thermal ablation, and triggering a powerful antitumor immune response that inhibits tumor recurrence. In this study, we designed a nanovaccine that combined PTT and immunotherapy for tumors using two-dimensional black phosphorus (BP) as a nanoplatform that was modified with maleimide poly(ethylene glycol) (PEG-MAL) and coated with tumor antigen proteins (BP-PEG-MAL@antigen).

Harnessing stimuli-responsive biomaterials for advanced biomedical applications.

Cell behavior is intricately intertwined with the in vivo microenvironment and endogenous pathways. The ability to guide cellular behavior toward specific goals can be achieved by external stimuli, notably electricity, light, ultrasound, and magnetism, simultaneously harnessed through biomaterial-mediated responses. These external triggers become focal points within the body due to interactions with biomaterials, facilitating a range of cellular pathways: electrical signal transmission, biochemical cues, drug release, cell loading, and modulation of mechanical stress.

Genetic Associations Between Specific Sleep-Related Phenotypes and Idiopathic Sudden Sensorineural Hearing Loss: A Mendelian Randomization Analysis.

Purpose: The relationship between idiopathic sleep-related phenotypes (SRPs) and sudden sensorineural hearing loss (ISSNHL) remains unclear. This study was designed to investigate the link between SRPs and ISSNHL from a genetic perspective through Mendelian randomization (MR) analysis.

Methods: ISSNHL trials were downloaded from Finngen database.

Analysis of prenatal diagnosis and pregnancy outcomes for rare autosomal trisomies detected by non-invasive prenatal testing in 33,079 cases.

Background: Non-invasive prenatal testing is widely used for screening common fetal aneuploidy disorders such as trisomy 21, trisomy 18, and trisomy 13. However, its ability to detect rare autosomal trisomies has introduced a new layer of complexity and clinical uncertainty.

Methods: A retrospective analysis was conducted on the prenatal diagnostic results and pregnancy outcomes of cases identified as high-risk for rare autosomal trisomies through non-invasive prenatal testing at the reproductive medicine center, Renmin hospital, Hubei university of medicine, from 2015 to 2023.

The expression of MALAT1, plasma brain natriuretic peptide, and Tei index in sepsis-induced myocardial injury.

Purpose: We sought to investigate the expression of MALAT1, plasma brain natriuretic peptide, and Tei index in sepsis-induced myocardial injury.

Methods: The current retrospective analysis focused on 146 sepsis patients admitted to our hospital from February 2021 to March 2023. Based on the presence or absence of myocardial injury, the patients were divided into two groups: the sepsis group (n = 80) and the sepsis-induced myocardial injury group (n = 66).

Identification of two novel genetic variants for Ellis-van Creveld syndrome from a Chinese family through whole exome sequencing.

Objective: This study conducted genetic testing and analysis on the fetal tissue of a terminated pregnancy to clarify the cause of the fetal abnormalities.

Methods: A fetus with multiple malformations detected during mid-pregnancy was terminated. Trio whole exome sequencing (Trio-WES) was performed on the fetal tissue.

Modulation of High-Frequency rTMS on Reward Circuitry in Individuals with Nicotine Dependence: A Preliminary fMRI Study.

Although previous studies have shown that repetitive transcranial magnetic stimulation (rTMS) can ameliorate addictive behaviors and cravings, the underlying neural mechanisms remain unclear. This study aimed to investigate the effect of high-frequency rTMS with the left dorsolateral prefrontal cortex (L-DLPFC) as a target region on smoking addiction in nicotine-dependent individuals by detecting the change of spontaneous brain activity in the reward circuitry. We recruited 17 nicotine-dependence participants, who completed 10 sessions of 10 Hz rTMS over a 2-week period and underwent evaluation of several dependence-related scales, and resting-state fMRI scan before and after the treatment.

Progestin-primed ovarian stimulation with letrozole using different doses of medroxyprogesterone acetate per day: a retrospective cohort study.

Background: In the progestin-primed ovarian stimulation protocol, the oral administration of medroxyprogesterone acetate has been observed to effectively inhibit the LH surge during ovarian stimulation in patients experiencing infertility. Nevertheless, the use of utilizing medroxyprogesterone acetate during ovarian stimulation can result in more pronounced pituitary suppression, potentially necessitating increased doses of gonadotropins and extended treatment durations. Therefore, it is necessary to determine the optimal dose of medroxyprogesterone acetate, aiming to use relatively lower concentrations of medroxyprogesterone acetate to effectively and safely suppress early LH surges.

Effect of the degree of follicular diameter ≥18mm differentiation on the day of hCG administration to the outcome of controlled ovarian hyperstimulation (COH).

Objective: To explore the impact of the level of differentiation in a minimum of two follicles with a diameter of ≥18 mm on the outcome of controlled ovarian hyperstimulation on the day of human chorionic gonadotropin (hCG) administration.

Methods: Single-center data from January 2018 to December 2021 was retrospectively analyzed for 1,199 patients with fresh embryo transfer for assisted reproduction. The absolute value of the standard deviation of the follicle size of at least 2 follicles ≥18 mm in diameter in both ovaries on the day of hCG was taken as the degree of differentiation of the dominant follicle after ovulation induction, based on the standard deviation response to the degree of dispersion of the data.

Reversible Fusion-Fission MXene Fiber-Based Microelectrodes for Target-Specific Gram-Positive and Gram-Negative Bacterium Discrimination.

Inaccurate or cumbersome clinical pathogen diagnosis between Gram-positive bacteria (G) and Gram-negative (G) bacteria lead to delayed clinical therapeutic interventions. Microelectrode-based electrochemical sensors exhibit the significant advantages of rapid response and minimal sample consumption, but the loading capacity and discrimination precision are weak. Herein, we develop reversible fusion-fission MXene-based fiber microelectrodes for G/G bacteria analysis.