Chemical proteomics reveals hesperidin and hesperetin as AKR1C1/2 inhibitors with chemosensitization effect.

The natural flavanone hesperidin and its aglycone hesperetin were identified as human aldo-keto reductase family 1 member C1 (AKR1C1) and AKR1C2 inhibitors chemoproteomic profiling, which provides mechanistic insight into their chemosensitization effects in cancer therapy.

Inhibiting effect of LIPUS on epithelial-mesenchymal transition in lens epithelial cells.

To investigate the effect of low-intensity pulsed ultrasound (LIPUS) on epithelial-mesenchymal transition (EMT) in lens epithelial cells. EMT was induced using high glucose (HG) in SRA01/04 cells. Optimal parameters for LIPUS irradiation were determined by cell counting kit-8 assays and flow cytometry.

Exploration of the optimal retention method in vivo for stem cell therapy: Low-intensity ultrasound preconditioning.

Bone marrow mesenchymal stem cells (BMSCs) are pluripotent and self-renewing, exerting a crucial role in the domain of regenerative medicine. Nevertheless, BMSCs encounter challenges such as low cell viability and inadequate homing during transplantation, thereby restricting their therapeutic efficacy. Hence, current research is concentrated on identifying optimal retention approaches following BMSCs transplantation to enhance its effectiveness.

The potential of low-intensity pulsed ultrasound to apply the long-term ovary protection from injury induced by 4-vinylcyclohexene diepoxide through inhibiting granulosa cell apoptosis.

The potential of low-intensity pulsed ultrasound (LIPUS) in regulating ovarian function has been demonstrated; however, there is a lack of scientific evidence regarding the long-term efficacy of LIPUS in treating ovarian injury and understanding its regulatory mechanisms. In this study, 4-vinylcyclohexene diepoxide (VCD) was used to induce ovarian injury in rats, and LIPUS was applied to target the damaged ovarian tissues. The research aimed to investigate the long-term protective effect of LIPUS against ovum toxicity induced by VCD and elucidate the associated molecular mechanisms.

Care-seeking and treatment pathways of multidrug-resistant tuberculosis patients: an analysis of real-world data from regional health information system in Ningbo City in Eastern China.

Objectives: The patient pathway of multidrug-resistant tuberculosis (MDR-TB) in China is an essential but not well-studied area. This study aimed to understand the alignment between patient-initiated care-seeking demands for MDR-TB and the availability of diagnostic and treatment services in Ningbo, a city in eastern China, using patient pathway analysis (PPA).

Methods: We collected the diagnostic and treatment data of 240 patients with MDR-TB in Ningbo from 2015 to 2019.

The ultrafine powder of atractylodis macrocephalae rhizoma improves immune function in naturally aging rats by regulating the PI3K/Akt/NF-κB signaling pathway.

Background: The phenomenon of population aging presents a significant global challenge, with the aging population in China steadily increasing. As individuals progress in age, there is a gradual deterioration of human organs and systems, as well as a decline in the immune system, referred to as immunosenescence. Atractylodis macrocephalae rhizoma (BZ) has been historically used in China for its medicinal properties, including gastrointestinal improvement, immunomodulation, anti-aging, antioxidant effects, and anti-tumor effects.

Mitophagy-mediated S1P facilitates muscle adaptive responses to endurance exercise through SPHK1-S1PR1/S1PR2 in slow-twitch myofibers.

Endurance exercise triggers adaptive responses especially in slow-twitch myofibers of skeletal muscles, leading to the remodeling of myofiber structure and the mitochondrial network. However, molecular mechanisms underlying these adaptive responses, with a focus on the fiber type-specific perspective, remains largely unknown. In this study we analyzed the alterations of transcriptomics and metabolomics in distinct skeletal myofibers in response to endurance exercise.

Metagenomic Next-Generation Sequencing-Assisted Diagnosis of Japanese Spotted Fever: Report of One Case.

Japanese spotted fever(JSF)is an infectious disease caused by ,with nonspecific clinical symptoms and a high risk of misdiagnosis.We reported a case of JSF,in which was detected in blood cells by metagenomic next-generation sequencing.The patient recovered after treatment with doxycycline.

Effects of herbicide mixtures on the diversity and composition of microbial community and nitrogen cycling function on agricultural soil: A field experiment in Northeast China.

Herbicide mixtures application is a widespread and effective practice in modern agriculture; however, a knowledge gap exists regarding the potential ecotoxicological effects of herbicide mixtures in agricultural systems. Here, the effects of various doses of herbicide mixtures (atrazine, nicosulfuron, and mesotrione) under different varieties of maize cultivation on the structure and function of microbial communities and soil chemical parameters were clarified through field experiments. The results showed that the application of herbicide mixtures increased the bacterial and fungal community alpha diversity at jointing and maturity, indicating a prolonged effect of the herbicide mixtures.

Deep Reinforcement Learning-Based Routing Method for Low Earth Orbit Mega-Constellation Satellite Networks with Service Function Constraints.

Low-orbit satellite communication networks have gradually become the research focus of fifth-generation (5G) beyond and sixth generation (6G) networks due to their advantages of wide coverage, large communication capacity, and low terrain influence. However, the low earth orbit mega satellite network (LEO-MSN) also has difficulty in constructing stable traffic transmission paths, network load imbalance and congestion due to the large scale of network nodes, a highly complex topology, and uneven distribution of traffic flow in time and space. In the service-based architecture proposed by 3GPP, the introduction of service function chain (SFC) constraints exacerbates these challenges.

BC99 Relieves Constipation Symptoms by Regulating Inflammatory, Neurotransmitter, and Lipid Metabolic Pathways: A Randomized, Double-Blind, Placebo-Controlled Trial.

Probiotics have attracted increasing attention due to their benefits in terms of relieving gastrointestinal ailments, including constipation. This study evaluated the potential of BC99 for clinical remission of constipation in adults. In this randomized, double-blind, and placebo-controlled trial, 90 individuals with constipation were divided between a BC99 and a placebo group for an 8-week intervention duration.

Age-period-cohort study of active pulmonary tuberculosis in eastern China: analysis of 15-year surveillance data.

Background: Tuberculosis remains a major public health challenge worldwide. This study aimed to determine the long-term trends in the notification rate of tuberculosis in Zhejiang Province, and to assess the potential independent risks associated with age, time period, and birth cohort.

Methods: Data on all pulmonary tuberculosis (PTB) cases in Zhejiang Province from 2008 to 2022 were collected from the Tuberculosis Information Management System.

Application of the age-period-cohort model in tuberculosis.

Up to now, tuberculosis (TB) remains a global public health problem, posing a serious threat to human health. Traditional methods for analyzing time-varying trends, such as age and period, tend to ignore the poor impact of birth cohorts, which is an important factor in the development of TB. The age-period-cohort (APC) model, a statistical method widely used in recent decades in economics, sociology, and epidemiology, can quantitatively estimate the efficacy of different age, period, and birth cohort groups for TB by separating the effects of these three dimensions and controlling for confounding factors among the time variables.

A phosphomolybdenum blue nano-photothermal agent with dual peak absorption and biodegradable properties based on ssDNA in near-infrared photothermal therapy for breast cancer.

Photothermal therapy (PTT) stands as an emerging and promising treatment modality and is being developed for the treatment of breast cancer, prostate cancer, and a series of superficial tumors. This innovative approach harnesses photothermal agents (PTAs) that convert near-infrared light (NIR) energy into heat, efficiently heating and ablating localized lesion tissue. Notably, the low scattering of NIR-II (1000-1500 nm) band light within biological tissue ensures superior penetration depth, surpassing that of NIR I (700-900 nm) band light.

Low-Intensity Pulsed Ultrasound Delays the Onset of Osteoporosis and Dyslipidemia in Mice With Premature Ovarian Insufficiency.

Objectives: The pathogenesis of premature ovarian insufficiency (POI) not only affects the ovarian structure and function but also gives rise to complications such as osteoporosis and dyslipidemia. Although low-intensity pulsed ultrasound (LIPUS) has been proven effective in treating POI, its impact on the associated complications remains unexplored. Therefore, this study aims to investigate the effects of LIPUS irradiation on osteoporosis and dyslipidemia in a mouse model of POI.

Natural epigallocatechin-3-gallocarboxylate nanoformulation loaded doxorubicin to construct a novel and low cardiotoxicity chemotherapeutic drug for high-efficiency breast cancer therapy.

Anthracycline doxorubicin (DOX) remains the first-line chemotherapeutic drug for the efficient treatment of breast cancer, but its severe cardiotoxicity limits its long-term application in clinical tumor chemotherapy. Until now, the pathogenesis mechanism of DOX-induced cardiotoxicity (DIC) is still not fully understood. According to current studies, the oxidative stress caused by the imbalance of reactive oxygen species (ROS) and reactive nitrogen species (RNS) production and mitochondrial dysfunction in myocardial cells are closely related to DIC.

Chemoproteomic Profiling Reveals Chlorogenic Acid as a Covalent Inhibitor of Dehydroascorbate Reductase 1.

Chlorogenic acid (CA) is an abundant plant secondary metabolite with promising allelopathic effects on weed growth. However, the molecular targets and mechanism of action of CA in plants remain elusive. Here, we report the employment of a clickable photoaffinity probe in identifying the protein targets of CA in seedling proteomes.

BC99 Improves Strength Performance by Enhancing Protein Digestion and Regulating Skeletal Muscle Quality in College Students of Physical Education Major.

The dietary proteins are one of the most important factors determining health conditions in humans. The sufficient digestion and absorption of dietary proteins in the digestive tract has positive effects on performance and recovery in sportspeople and athletes. Improving protein digestibility is a strategy for maintaining health status and optimal performance in sport and exercise activities.

Psittacosis Pneumonia Features, Distinguishing Characteristics, and Outcomes: A Retrospective Study.

Purpose: Psittacosis is an often-neglected cause of community acquired pneumonia (CAP). The limited diagnostic methods for psittacosis pneumonia invariably result in an unfavourable prognosis. Consequently, the early detection of psittacosis pneumonia is crucial.

Quantum Melting of Generalized Wigner Crystals in Transition Metal Dichalcogenide Moiré Systems.

The generalized Wigner crystal (GWC) is a novel quantum phase of matter driven by further-range interaction at fractional fillings of a lattice. The role of further-range interaction as the driver for the incompressible state is akin to the Wigner crystal. On the other hand, the significant role of commensurate filling is akin to the Mott insulator.

Polydopamine Nanoformulations Induced ICD and M1 Phenotype Macrophage Polarization for Enhanced TNBC Synergistic Photothermal Immunotherapy.

Photothermal therapy (PTT) is a promising technology that can achieve the thermal ablation of tumors and induce immunogenic cell death (ICD). However, relying solely on the antitumor immune responses caused by PTT-induced ICD is insufficient to suppress tumor metastasis and recurrence effectively. Fortunately, multifunctional nanoformulation-based synergistic photothermal immunotherapy can eliminate primary and metastatic tumors and inhibit tumor recurrence for a long time.

Attention-Guided Sample-Based Feature Enhancement Network for Crowded Pedestrian Detection Using Vision Sensors.

Occlusion presents a major obstacle in the development of pedestrian detection technologies utilizing computer vision. This challenge includes both inter-class occlusion caused by environmental objects obscuring pedestrians, and intra-class occlusion resulting from interactions between pedestrians. In complex and variable urban settings, these compounded occlusion patterns critically limit the efficacy of both one-stage and two-stage pedestrian detectors, leading to suboptimal detection performance.