Electroacupuncture combined with botulinum toxin type A promotes common peroneal nerve repair and activation of GDNF-BDNF/TrkB signaling pathway in post-stroke limb spasticity rats.

Background: Post-stroke limb spasticity (PSLS) is a rehabilitation problem that needs to be solved urgently, electroacupuncture (EA), a safe and effective adjunctive rehabilitation therapy with relatively few side effects, and botulinum toxin type A (BTX-A) can improve motor function; however, it is not clear whether EA combined with BTX-A is more effective in improving PSLS. This study will investigate the effects and mechanisms of EA combined with BTX-A to improve PSLS in rats.

Methods: PSLS rat model was prepared and treated with EA, BTX-A, or EA+BTX-A, Zea longa, modified Ashworth, and modified neurological severity score to assess the changes of neurological function and dystonia, and to observe the cerebral infarction condition, fibers changes of gastrocnemius muscle, common peroneal nerve pathology morphology, changes of neural repair factors and GDNF/BDNF/TrkB signaling pathway in the rats, respectively.

Sleep Deprivation Activates a Conserved Lactate-H3K18la-RORα Axis Driving Neutrophilic Inflammation Across Species.

Sleep deprivation critically disrupts physiological homeostasis, impairing development, metabolic balance, and immune regulation, with excessive neutrophil activation being a hallmark consequence. However, the molecular mechanisms underlying sleep deprivation-induced neutrophilic inflammation remain elusive. Here, it is shown that acute sleep deprivation in mice triggers neutrophil hyperactivation, resulting in aberrant peripheral accumulation and a systemic cytokine storm.

AdaGCL+: An Adaptive Subgraph Contrastive Learning Toward Tackling Topological Bias.

Large-scale graph data poses a training scalability challenge, which is generally treated by employing batch sampling methods to divide the graph into smaller subgraphs and train them in batches. However, such an approach introduces a topological bias in the local batches compared with the complete graph structure, missing either node features or edges. This topological bias is empirically shown to affect the generalization capabilities of graph neural networks (GNNs).

High HPV viral load predicts worse prognosis in patients with cervical cancer treated with radiochemotherapy.

Purpose: To evaluate the prognostic value of high-risk human papillomavirus (HR-HPV) viral load in long-term survival of cervical cancer (CC) patients.

Methods: Two hundred and twenty-nine CC patients from 2009 to 2013 who underwent radiochemotherapy (RCT) or plus hyperthermia (HT) were recruited. HPV type 16, 18, 58 DNA was detected by in situ hybridization (ISH) and either HR-HPV type with the highest intensive signals was chosen as the evaluating target.

An intelligent system for precise management of coagulants in sludge conditioning: Inspired by the exploratory behavior in primates using senses.

Coagulation conditioning is a key step for sludge dewatering, while the control of optimal coagulant dosing is still a challenge. This study proposes an intelligent system for the precise management of coagulants (IIS) in sludge conditioning. This system was assembled using an electrical impedance spectroscopy (EIS)-based sensor and a decision-making model to conduct the self-perception, decision-making, execution, and dosage optimization in sludge conditioning through a feedback-driven exploratory trial process, conceptually inspired by structured exploration behavior observed in primates.

Dissolved organic matter mediates the interactions between bacterial community and heavy metal fractionation in contaminated coal mine soils.

Heavy metal (HM) contamination in coal mine soils disrupts local bacterial networks, leading to prolonged soil deterioration. Dissolved organic matter (DOM), a crucial soil component, actively modulates both bacterial metabolism and HM mobilization. Despite its significance, our understanding of the complex interactions among bacterial communities, soil chemical and DOM properties, and HM fractionation remains limited.

Advances in the Treatment of Spinal Cord Injury with Nanozymes.

Spinal cord injury (SCI) with increasing incidence can lead to severe disability. The pathological process involves complex mechanisms such as oxidative stress, inflammation, and neuron apoptosis. Current treatment strategies focusing on the relief of oxidative stress and inflammation have achieved good effects, while many problems and challenges remain such as the side effect and short half-life of the therapeutic agents.

Balancing user preferences by social networks: A condition-guided social recommendation model for mitigating popularity bias.

Social recommendation models weave social interactions into their design to provide uniquely personalized recommendation results for users. However, social networks not only amplify the popularity bias in recommendation models, resulting in more frequent recommendation of hot items and fewer long-tail items, but also include a substantial amount of redundant information that is essentially meaningless for the model's performance. Existing social recommendation models often integrate the entire social network directly, with little effort to filter or adjust social information to mitigate popularity bias introduced by the social network.

Building Powerful Zinc-Ion Hybrid Capacitors by an Energy Drink-Inspired Strategy.

Numerous modification strategies have been proposed to enhance the performance of the Zn anode and carbon cathode in aqueous zinc-ion hybrid capacitors (ZIHCs). However, one efficient strategy to modify both the anode and cathode is still lacking. Herein, taurine (Tau), the key ingredient of energy drinks, is used as the electrolyte additive and carbon precursor for ZIHCs simultaneously.

ROS regulates circadian rhythms by modulating Ezh2 interactions with clock proteins.

Redox imbalance induced by the accumulation of reactive oxygen species (ROS) accelerates age-related processes, often accompanied by a decrease in circadian rhythm amplitude. However, the underlying mechanisms by which ROS modulate circadian rhythms remain poorly understood. In this study, we found that ROS disrupt circadian rhythms in both zebrafish, as indicated by changes in diurnal behavior and clock gene expression, and in a human cell model.

Case report: Complete response in TMB-H advanced uterine clear cell carcinoma: a case analysis of paclitaxel albumin-bound combined with PD-1/CTLA-4 bispecific antibody.

Background: Uterine clear cell carcinoma (UCCC) is a rare and aggressive subtype of endometrial cancer, often presenting at an advanced stage with poor prognosis. Treatment options for advanced or recurrent UCCC are currently limited, especially after platinum-based chemotherapy has failed.

Case Presentation: We present the case of a 49-year-old female diagnosed with stage IV uterine clear cell carcinoma.

Metal Transfer Behavior and Molten Pool Dynamics in Cold Metal Transfer Pulse Advanced Additive Manufacturing of 7075 Aluminum Alloy.

Wire arc additive manufacturing (WAAM) with a special arc mode of cold metal transfer pulse advanced (CMT-PADV) is an ideal additive manufacturing process for fabricating aerospace components, primarily high-strength aluminum alloys, offering advantages such as high deposition rates and low cost. However, the numerical simulation of the CMT-PADV WAAM process has not been researched until now. In this study, we first developed a three-dimensional fluid dynamics model for the CMT-PADV WAAM of 7075 aluminum alloy, aiming at analyzing the droplet transition and molten pool flow.

[Curcumol Mediates the Programmed Cell Death in Acute Myeloid Leukemia through PI3K/AKT Signaling Pathway].

Objective: To investigate the effects of Curcumol on the malignant biological characteristics of acute myeloid leukemia (AML) cells and its molecular mechanism, and to provide theoretical and experimental evidence for the anti-leukemia treatment of traditional Chinese medicine.

Methods: After the AML cell lines HL-60 and KG-1 cells were treated different concentrations of with Curcumol. The proliferation activity of cells was detected by CCK-8 method, and the expression changes of apoptotic proteins and PI3K/AKT signaling pathway proteins were detected by Western blot.

Lanthanum hydroxide showed best application potential in La-based materials based on its stable phosphate adsorption properties in complex water environments.

Three lanthanum morphology materials encompassing all lanthanum composites were synthesized by the direct precipitation method, and their phosphate adsorption order was determined as La(CO) > La(OH) > LaO. Further comparison of the adsorption performance between La(CO) and La(OH) revealed that the former exhibited a twofold higher rate of adsorption compared to the latter. The presence of SO, HCO, Mg, and HA in the water led to a decrease in the phosphate adsorption of La(CO) and La(OH), while Ca enhances the adsorption of phosphoric acid by both materials.

Comprehensive analysis and novel insights into the efficacy of polymyxin B sulfate in the treatment of sepsis caused by carbapenem-resistant gram-negative bacteria.

Objective: To investigate and analyze the clinical efficacy and safety of polymyxin B sulfate in the treatment of carbapenem-resistant gram-negative bacteria (CR-GNB) in sepsis; in order to provide reference for the clinical diagnosis, treatment and prognosis evaluation of sepsis.

Methods: The clinical data of 76 patients with CR-GNB sepsis treated with polymyxin B sulfate combined with an anti-infection regimen in the First Affiliated Hospital of Gannan Medical University from January 2020 to February 2024 were retrospectively studied. To analyze and discuss the clinical characteristics, results of the bacterial culture and drug sensitivity, clinical efficacy and prognosis of CR-GNB patients, efficacy comparison of different doses of polymyxin B sulfate treatment regimens, efficacy comparison of different combination regimens based on polymyxin B sulfate, changes in clinical indexes before and after treatment of polymyxin B sulfate, adverse drug reactions and adverse events of polymyxin B sulfate were investigated.

3D-printed brachytherapy in patients with cervical cancer: improving efficacy and safety outcomes.

Objective: This study aims to evaluate the efficacy and safety of 3D printing technology in brachytherapy for cervical cancer, comparing its outcomes with conventional free hand implantation brachytherapy.

Methods: A total of 50 cervical cancer patients treated at the First Affiliated Hospital of Gannan Medical College from January 2019 to July 2023 were included in this study. Patients were divided into two groups: 25 patients received intensity-modulated radiotherapy (IMRT) combined with 3D-printed brachytherapy, and 25 patients underwent IMRT combined with free hand brachytherapy implantation.

[Mechanism of Huayu Jiedu Decoction in Inhibiting Malignant Biological Characteristics of Multiple Myeloma].

Objective: To analyze and explore the effects of Huayu Jiedu Decoction on the malignant biological characteristics of multiple myeloma (MM) cells and its molecular mechanism, so as to provide experimental basis and theoretical basis for the alternative therapy of anti-MM in traditional Chinese medicine.

Methods: Different concentrations of Huayu Jiedu Decoction were used to intervene myeloma U266 cells. The changes of cell proliferation activity were detected by CCK-8 assay, apoptosis was detected by Annexin V/PI double staining flow cytometry, and apoptosis and protein expression of related signaling pathways were detected by Western blot.

Phase II study of enlonstobart (SG001), a novel PD-1 inhibitor in patients with PD-L1 positive recurrent/metastatic cervical cancer.

Background: Platinum-based chemotherapy with or without bevacizumab is the first-line treatment for patients with recurrent or metastatic cervical cancer (r/mCC), and the treatment options are limited for r/mCC after first-line treatment. Enlonstobart (SG001) is a fully humanized and high-affinity anti-PD-1 immunoglobulin G4 monoclonal antibody. Previous phase Ib study demonstrated that SG001 had a promising efficacy in patients with PD-L1 positive r/mCC.

Clinical Grade Fibroin Sutures with Bioactive Gold Clusters Enhance Surgical Wound Healing via Inflammation Modulation.

Silk sutures are common in surgeries, and silk-based textiles are widely used in clinical medicine on account of their great mechanical properties and biodegradability. However, due to the lack of biocatalytic activity, silk sutures show unsatisfactory anti-inflammatory properties and healing speed. To address this constraint, we construct clinical grade bioactive gold cluster-sutures through a heterojunction.

Exploring microbial diversity and function in companion planting systems of white clover and orchard grass.

Companion planting of white clover (Trifolium repens L.) with orchard grass (Dactylis glomerata L.), a famous hay grass, improves the forage quality of orchard grass.

Atomic Artificial Enzyme for Acute and Chronic Pneumonia.

Pneumonia involves complex immunological and pathological processes leading to pulmonary dysfunction, which can be life-threatening yet lacks effective specialized medications. Natural enzymes can be used as biological agents for the treatment of oxidative stress-related diseases, but limiting to catalytic and environmental stability as well as high cost. Herein, an artificial enzyme, gold nanoclusters (Au NCs) with excellent stability, bioactivity, and renal clearance can be used as the next-generation biological agents for acute lung injury (ALI) and allergic lung disease (ALD).

Public health use and lessons learned from a statewide SARS-CoV-2 wastewater monitoring program (MiNET).

The global SARS-CoV-2 monitoring effort has been extensive, resulting in many states and countries establishing wastewater-based epidemiology programs to address the spread of the virus during the pandemic. Challenges for programs include concurrently optimizing methods, training new laboratories, and implementing successful surveillance programs that can rapidly translate results for public health, and policy making. Surveillance in Michigan early in the pandemic in 2020 highlights the importance of quality-controlled data and explores correlations with wastewater and clinical case data aggregated at the state level.

Primary intestinal diffuse large B-cell lymphoma: novel insights and clinical perception.

Background: Extranodal Non-Hodgkin lymphoma (NHL) is more prevalent in the gastrointestinal (GI) tract than in other sites. This study aimed to explore the clinical features and prognostic factors of primary intestinal diffuse large B-cell lymphoma (PI-DLBCL), in order to provide new references for basic research and clinical diagnosis and treatment of the rare extranodal malignant lymphoma.

Methods: The clinical data of 88 patients with PI-DLBCL admitted to Fujian Medical University Union Hospital from June 2011 to June 2022 were retrospectively studied, the clinical and pathological features, diagnosis and treatment process and prognosis of PI-DLBCL were analyzed, and univariate and multivariate analysis of prognostic factors was carried out.

Using An In Vitro Tissue Perfusion System to Detect the Functional Activities of Isolated Intestinal Tubes in Real Time.

Gastrointestinal diseases, which have a high incidence, pose considerable challenges for humans. The small intestine is integral to food and drug digestion and absorption and plays a crucial role in treating these diseases. The intestinal tube movement experiment, a common and essential in vitro method, is utilized to study gastrointestinal dynamics.