Tissue-specific endophytic microbiome responses to " Liberibacter asiaticus" in two citrus cultivars.

Citrus Huanglongbing (HLB), caused by "Candidatus Liberibacter asiaticus" (CLas), is a destructive disease threatening global citrus industry. Although citrus cultivars differ in HLB sensitivity, how infection alters endophytic bacterial communities in cultivars with contrasting susceptibility remains unclear. Here, we compared endophytic microbiome shifts in leaf and root tissue of HLB-susceptible Shatangju mandarin (C.

Open Challenges and Opportunities in Piezoelectricity for Tissue Regeneration.

Emerging piezoelectric biomaterials have demonstrated their huge potential in diverse medical applications, including ultrasound diagnosis and tissue regeneration. Human body possesses inherent piezoelectricity, producing electrical signal under endogenous load or external pressure to modulate cellular behaviors. Tissue defects caused by traumatic injury will disrupt the electrophysiological microenvironment of the injured area, resulting in unenviable self-healing.

Transcriptomic Reprogramming and Key Molecular Pathways Underlying Huanglongbing Tolerance and Susceptibility in Six Citrus Cultivars.

Huanglongbing (HLB), caused by Liberibacter asiaticus (CLas), is the most devastating disease threatening global citrus production. Although no commercial citrus varieties exhibit complete HLB resistance, genotype-specific tolerance variations remain underexplored. This study conducted a comparative transcriptomic profiling of six commercially citrus cultivars in South China, four susceptible cultivars ( cv.

Reinforcement learning-based event-driven optimal prevention control strategy for citrus huanglongbing model.

Citrus Huanglongbing (HLB) is an infectious disease transmitted by Asian citrus psyllids (ACP), which leads to serious economic losses in the citrus industry. Therefore, it is crucial to investigate the prevention and control strategy of citrus HLB. In this paper, the dynamics of HLB propagation between citrus trees and ACP is considered.

Individual and dyadic network analysis of anxiety and depression among infertile couples: A cross-sectional study.

Purpose: This study aims to explore anxiety and depression symptom networks in infertile couples, focusing on sex differences and the transmission of psychological distress between partners.

Methods: This cross-sectional study recruited patients from the First Affiliated Hospital of Soochow University between October 2021 and April 2024. Participants completed the Generalized Anxiety Disorder 7-Item Scale and the Patient Health Questionnaire-9.

A three-tier AI solution for equitable glaucoma diagnosis across China's hierarchical healthcare system.

Artificial intelligence (AI) offers a solution to glaucoma care inequities driven by uneven resource distribution, but its real-world implementation remains limited. Here, we introduce Multi-Glau, an three-tier AI system tailored to China's hierarchical healthcare system to promote health equity in glaucoma care, even in settings with limited equipment. The system comprises three modules: (1) a screening module for primary hospitals that eliminates reliance on imaging; (2) a pre-diagnosis module for handling incomplete data in secondary hospitals, and (3) a definitive diagnosis module for the precise diagnosis of glaucoma severity in tertiary hospitals.

Piezoelectricity Regulating Immune Osteogenesis in Osteoporosis.

This study aims to investigate the regulatory effects of piezoelectricity on the inflammatory microenvironment in osteoporosis treatment. Recent studies have extensively explored the impact of piezoelectric materials on macrophage polarization and cytokine secretion. However, the effects of piezoelectricity on macrophages for the regulation of immune osteogenesis in osteoporosis remain poorly understood.

Orchestration of Gut-Liver-Associated Transcription Factors in MAFLD: From Cross-Organ Interactions to Therapeutic Innovation.

Metabolic dysfunction-associated fatty liver disease (MAFLD) represents a global health burden, however, therapeutic advancements remain hindered by incomplete insights on mechanisms and suboptimal clinical interventions. This review focused on the transcription factors (TFs) associated with the gut-liver axis, emphasizing their roles as molecular interpreters of systemic crosstalk in MAFLD. We delineate how TF networks integrate metabolic, immune, and gut microbial signals to manage hepatic steatosis, inflammation, and fibrosis.

Cytohesin-4/ARF6 facilitates the progression of acute myeloid leukemia through activating PIK3R5/PI3K/AKT pathway.

analysis revealed an elevated expression of cytohesin-4 (CYTH4) in acute myeloid leukemia (AML) cells, correlating with a poorer prognosis for AML patients. However, its role in AML is not fully understood. Our study using loss-of-function assays identified CYTH4 as an oncogene promoting leukemogenesis.

Genomic Diversity of Phage CLasMV1 in " Liberibacter asiaticus".

A phage CLasMV1, was recently identified in " Liberibacter asiaticus" (CLas), the bacterium associated with citrus Huanglongbing (HLB). The CLasMV1 strain GDHZ11D (CP045566) exhibited a circular genome of 8,869 bp containing eight open reading frames (ORFs). Phages of CLas play crucial roles in regulating bacterial population diversity that have potential use for HLB control.

Activated Immune and Complement C3 Are Potential Contributors in MASH via Stimulating Neutrophil Extracellular Traps.

The number of metabolic dysfunction-associated steatotic liver disease (MASLD) patients is increasing rapidly. More attention has been paid to the relationship between immunity and MASLD. This study explored the roles of serum autoantibodies, immunoglobulins, and complements in MASLD.

Application of novel dark-quencher labeled probes in multiplex qRT-PCR assays for rapid detection of SARS-CoV-2 variants.

Background: Coronavirus disease 2019 (COVID-19) is an acute infectious disease caused by the new coronavirus, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Because SARS-CoV-2 frequently mutates, it creates a number of variants that must be distinguished and tracked using a rapid detection technique. At present, the identification of virus variants often requires sequencing of the viral genome with sophisticated techniques which are costly and time-consuming.

New polyketides from the mangrove-associated endophytic fungus sp. DHS-11.

Chemical investigation of the mangrove-derived fungus sp. DHS-11 led to the isolation of three new pyrone derivatives (-), and a new natural product (). Their structures were identified by NMR spectroscopy and mass spectrometry.

SRF and CBP jointly regulate integrin β6 overexpression in head and neck squamous cell carcinomas.

Overexpression of integrin β6 (ITGB6) is crucially linked to the invasion and metastasis of head and neck squamous cell carcinoma (HNSCC). The molecular mechanisms driving ITGB6 upregulation in HNSCC are not well understood. Our study comprehensively analyzed the transcriptional regulation and epigenetic modification mechanisms affecting ITGB6 transcription.

Targeting circFOXO3 to Modulate Integrin β6 Expression in Periodontitis: A Potential Therapeutic Approach.

Aims: Circular RNA forkhead box O3 (circFOXO3) is crucial in regulating inflammation in lung and heart injuries. However, its role in periodontitis remains unclear. We sought to elucidate the effects of circFOXO3 on periodontitis progression and related molecular mechanisms.

Deficiency of Epithelial PIEZO1 Alleviates Liver Steatosis Induced by High-Fat Diet in Mice.

PIEZO1 has been found to play a vital role in regulating intestinal epithelial cells (IEC) function and maintaining intestinal barrier in recent years. Therefore, IEC PIEZO1 might exert a significant impact on liver metabolism through the gut-liver axis, but there is no research on this topic currently. Classic high-fat diet (HFD) model and mice with IEC-specific deficiency of PIEZO1 ( ) were used to explore the problem.

Mechanical and diffusion property and multi-objective optimization of rubber seals exposed to high-pressure hydrogen gas.

As the critical components in hydrogen refueling, storage, and transportation systems, the degradation and failure of rubber O-ring seals under a high-pressure (HP) hydrogen environment (up to 100 MPa) directly affect hydrogen energy safety. Clarifying the interaction mechanism of hydrogen diffusion and the mechanical properties of rubber seals is essential for HP hydrogen infrastructure. A hydrogen diffusion-mechanical sequential numerical model is built to investigate the sealing performance and hydrogen diffusion behaviors of rubber seals using ABAQUS software.

Multifunctional Temperature-Sensitive Lipid-Protein-Polymer Conjugates: Tailored Drug Delivery and Bioimaging.

In this study, we introduce a protein-polymer bioconjugate comprising bovine serum albumin (BSA) and a lipid-based thermoresponsive block copolymer. These amphiphilic BSA-polymer conjugates can autonomously be organized into vesicular compartments for codelivery of glucose oxidase (GOx) and doxorubicin (DOX), demonstrating high drug loading content and remarkable antitumor activity via synergistic cancer therapy combining chemo-starvation strategies. Through the incorporation of a hydrophilic BSA block, the lower critical solution temperature (LCST) of the bioconjugates is tuned to around 40 °C, facilitating their targeted drug delivery to tumor cells.

Hierarchical Ordered Mesoporous SrBiTiO Microflowers with Rich Oxygen Vacancies In Situ Assembled by Nanosheets for Piezo-Photocatalysis.

The Aurivillius phase layered perovskite ferroelectric material SrBiTiO (SBTO) exhibits spontaneous polarization and piezoelectric properties, which confer significant potential for piezo-photocatalysis. Its ability to enhance electron-hole separation while providing excellent fatigue resistance positions it as a promising candidate in this field. Defects were introduced to improve the structural polarization and photoelectrochemical properties of SBTO.

Maintaining mitochondrial DNA copy number mitigates ROS-induced oocyte decline and female reproductive aging.

Oocytes play a crucial role in transmitting maternal mitochondrial DNA (mtDNA), essential for the continuation of species. However, the effects of mitochondrial reactive oxygen species (ROS) on mammalian oocyte maturation and mtDNA maintenance remain unclear. We investigated this by conditionally knocking out the Sod2 gene in primordial follicles, elevating mitochondrial matrix ROS levels from early oocyte stages.

Genomic Analysis of ' Liberibacter africanus' Strain from Zimbabwe Reveals Unique Virulence and Prophage Characteristics Compared with ' L. asiaticus'.

Citrus Huanglongbing (HLB) is caused by the phloem-limited α-proteobacterium ' Liberibacter spp.', among which ' L. africanus' (CLaf) has posed a significant threat to citrus production in Africa for nearly a century.

Global citrus root microbiota unravels assembly cues and core members.

Introduction: Citrus is one of the most important fruit crops worldwide, and the root-associated microbiota can have a profound impact on tree health and growth.

Methods: In a collaborative effort, the International Citrus Microbiome Consortium investigated the global citrus root microbiota with samples collected from nine citrus-producing countries across six continents. We analyzed 16S rDNA and ITS2 amplicon sequencing data to identify predominant prokaryotic and fungal taxa in citrus root samples.

An unconstrained palmprint region of interest extraction method based on lightweight networks.

Accurately extracting the Region of Interest (ROI) of a palm print was crucial for subsequent palm print recognition. However, under unconstrained environmental conditions, the user's palm posture and angle, as well as the background and lighting of the environment, were not controlled, making the extraction of the ROI of palm print a major challenge. In existing research methods, traditional ROI extraction methods relied on image segmentation and were difficult to apply to multiple datasets simultaneously under the aforementioned interference.

Comparative efficacy and safety of intelligent pressure-controlled versus flexible vacuum-assisted ureteral access sheath for 2-4 cm renal calculi.

Background: Retrograde intrarenal surgery (RIRS) is being increasingly used to treat 2-4 cm renal stones, which can be attributed to advances in flexible ureteroscopes and ureteral access sheaths (UASs). Despite the improvement and application of flexible vacuum-assisted (FV) and intelligent pressure-controlled (IPC) UASs, no studies have compared their therapeutic efficacy and safety. Therefore, this study aimed to compare the therapeutic efficacy and safety of IPC-UAS and FV-UAS in RIRS 2-4 cm renal stones.