A hydrogel/P(VDF-TrFE) system for enhanced bone regeneration with controllable self-sustained electrical cues.

The limited self-healing capacity of critical-sized bone defects presents significant challenges in healing. An effective approach is to regulate the physicochemical properties of biomaterials to mimic the natural bone regenerative microenvironment. In this work, we have prepared Chitosan-Gelatin (CS-Gel) based hydrogel/ Poly(vinylidene fluoride-co-trifluoroethylene) (P(VDF-TrFE)) systems, which provide biomimetic and electric cues for bone regeneration.

Co-Radiotherapy with Tyrosine Kinase Inhibitors Might Benefit Survival in Hepatoma Patients with Portal Vein Tumor Thrombosis.

Background: Hepatocellular carcinoma (HCC) with portal vein tumor thrombus (PVTT) is associated with a poor prognosis, and current treatment options yield suboptimal results. This article aims to share our treatment outcomes for PVTT using tyrosine kinase inhibitors (TKIs), radiation therapy (RT), and their combination.

Methods: From 2010 to 2021, a total of 160 Child-Pugh class A HCC patients with PVTT, but without extrahepatic spread, were enrolled.

Aerosol sampling-based infection risk model for spatiotemporal resolution informed evaluation of on-site transmission risk.

Accurate quantification of on-site airborne transmission risk is critical for epidemic prevention and control. Environmental modeling study is hard for on-site monitoring of pathogens while instrument-based detection face barriers to evaluate potential risk directly. This study developed an aerosol sampling-based infection risk model (AS-IRM) that integrates the instruments, collection, and detection into the risk evaluation model.

Chiral Hydroxyapatite Coatings for Improving Bone In-Growth of Porous Tantalum Scaffold via FAK-Notch1 Signaling Pathway.

With the rising prevalence of osteoarthritis, joint arthroplasty is becoming increasingly common in clinical practice. Prosthesis loosening is a primary factor contributing to the failure of joint arthroplasty, closely linked to inadequate bone in-growth. In recent years, porous tantalum has increasingly become a widely used anti-loosening material due to its good biocompatibility and mechanical properties.

Deicing Mechanisms of Two-Phase Skeleton/Gel Composites with Tunable Surface Moduli.

Developing durable ice-phobic materials is essential for minimizing ice-related hazards in aerospace, transportation, and energy infrastructure systems. In this study, two types of porous skeleton-gel composites with distinct surface moduli were fabricated, i.e.

Electro-switchable addressing system for achieving repetitive random data access.

Due to its high physical density and excellent durability, DNA data storage has become an attractive storage solution. Efficient and fast random access to the required storage data is a key element of any data storage system. Here, we present a method of using electrically driven DNA conformation to promote or inhibit T7 promoter transcription in order to random access DNA information.

Development of a Monoclonal Antibody-Based Colloidal Gold Immunochromatographic Strip for Detection of Feline Coronavirus Infections.

This study aimed to generate a monoclonal antibody (mAb) against feline coronavirus (FCoV) spike (S) protein and to develop a colloidal gold immunochromatographic strip for the rapid and accurate FCoV detection. BALB/c mice were immunized with the purified protein, and hybridoma technology was employed to produce highly effective mAb. A positive hybridoma cell line (E5) was identified, which stably secreted mAb with a high titer of 1:256000 against the FCoV S recombinant protein.

Scaling the High-Yield Potential of Large-Scale DNA Data Storage with Cap-Free DNA Synthesis.

DNA has emerged as a promising storage medium for addressing exponentially growing data storage demands, owing to its exceptional information density and chemical stability. However, current DNA synthesis techniques face significant limitations in achieving high-throughput data storage due to constrained synthesis yields. This study presents a novel high-yield cap-free synthesis (HYCFS) strategy that overcomes the limitations of conventional solid-phase phosphoramidite chemistry in both synthesis length and production yield.

Light-Regulated P(VDF-TrFE)/BiS Microneedle Patch with Tunable Electric Stimulation for Modulating the Healing of Burn Wound Dynamically.

As the skin wound forms, external electric stimulation (ES) is required to mimic the endogenous electric field that has been damaged, in order to mediate cellular behavior and promote tissue healing. At the same time, there are certain differences in the external electric stimulation requirements at different stages of skin wound healing. In this study, a Poly(vinylidene fluoride-co-trifluoroethylene)/Bismuth sulfide (P(VDF-TrFE)/Bi₂S₃) microneedle (MN) patch that is able to provide external ES to damaged tissue is developed.

Multifunctional Sr/Zn Co-Doped Mesoporous Silica Nanoparticles in Injectable Hydrogel for Ameliorating Osteoporotic Osseointegration.

Developing advanced biomaterials with controllable nanostructures and biological multifunctionality is highly promising in biomedical fields. In this study, novel Sr/Zn co-doped mesoporous silica nanoparticles (MSNs) are fabricated using a designated substitution and etching method. The obtained SrZn-MSNs possess unique hollow mesoporous structures, round spherical morphology, high specific surface areas, and suitable pore sizes.

DNA Logic-Integrated Quantum Nanosensor for MicroRNA Diagnostics.

Nanodiamonds (NDs) with nitrogen-vacancy (NV) centers are emerging as powerful quantum nanosensors (QNs) in biomedical applications due to their exceptional sensitivity. However, achieving optimal diagnostics performance necessitates both high sensitivity and selectivity; especially in practical biomedical settings, it remains challenging for QNs to provide quantitative analyses when multiple analytes are present. Here, we present a biosensing platform that integrates DNA logic gates (DLGs) with spin-based quantum sensing, termed DLG-QN for ultrasensitive and ultraselective diagnostics.

Metabolic and immune heterogeneity in stroke: Insights into subtype identification and biomarker discovery.

Stroke is a leading cause of mortality and long-term disability worldwide, characterized by substantial molecular and clinical heterogeneity. This study aimed to investigate the metabolic and immune-related mechanisms underlying stroke subtypes using transcriptomic data and advanced computational tools. Gene expression data from two GEO datasets (GSE16561 and GSE58294) were preprocessed, batch-corrected, and integrated.

Inflammatory Cell Interactions in the Rotator Cuff Microenvironment: Insights From Single-Cell Sequencing.

Rotator cuff injuries are a common cause of shoulder pain and dysfunction, with chronic inflammation complicating recovery. Recent advances in single-cell RNA sequencing (scRNA-seq) have provided new insights into the immune cell interactions within the rotator cuff microenvironment during injury and healing. This review focuses on the application of scRNA-seq to explore the roles of immune and nonimmune cells, including macrophages, T-cells, fibroblasts, and myofibroblasts, in driving inflammation, tissue repair, and fibrosis.

Medial Compartment Knee Osteoarthritis Altered Tibiofemoral Joint Kinematics and Contact Pattern During Daily Weight-Bearing Extension.

Objective: With the advancement of digital orthopedics, the growing prevalence of medial compartment knee osteoarthritis (MCKOA) and the widespread adoption of knee-preserving surgical techniques have heightened new interest in predicting the onset of MCKOA and promoting surgical outcomes. This study was to clarify the differences in kinematics and contact patterns between the MCKOA knee and its native sides during knee extension.

Methods: From March 2023 to June 2024, thirty-two patients who suffered from unilateral MCKOA, with their contralateral extremities asymptomatic and intact, were enrolled in this descriptive research.

Injectable 2D-MoS-integrated Bioadhesive Hydrogel as Photothermal-Derived and Drug-Delivery Implant for Colorectal Cancer Therapy.

Photothermal therapy (PTT) combined with chemotherapy using hydrogel as a delivery platform is considered a promising strategy for the treatment of advanced colorectal cancer (CRC). However, maintaining the stability of photo-absorbing agents (PTA) in the hydrogel and ensuring that the hydrogel remains anchored to the tumor tissue presents significant challenges. Herein, this work introduces an injectable 2D molybdenum disulfide (2D-MoS)-integrated adhesive hydrogel, specifically N-(2-aminoethyl)-4-(4-(hydroxymethyl)-2-methoxy-5-nitro-sophenoxy) butanamide-linked sodium alginate-MoS-5-fluorouracil (AlgNB/MoS/5-FU).

Digging into the Cause of Abnormal Patellar Kinematics After Open-Wedge High Tibial Osteotomy via a Quantitative Study on In Vivo Soft Tissue Functional Changes.

The biomechanical mechanism of postoperative patellofemoral joint (PFJ) complications after open-wedge high tibial osteotomy (OWHTO) has not been investigated. This study was to determine the length changes in the patellar tendon (PT), medial patellotibial ligament (MPTL), medial patellofemoral ligament (MPFL), and quadriceps moment arm (QMA) during staircase motion before and after OWHTO. Computed tomography (CT) scans of 15 patients' lower extremities were used to reconstruct three-dimensional models, and magnetic resonance imaging (MRI) of the knee and hip joints was used to mark the soft tissue footprints.

HKA angle exceeding 5 degrees is strongly associated with lateral patellar translation beyond 2 mm: surgical recommendations for avoiding adverse effects on the patellofemoral joint after OWHTO.

Purpose: Previous studies reported that anterior knee pain (AKP) occurs with an incidence of 32% after opening-wedge high tibial osteotomy (OWHTO). However, the biomechanical effects of this procedure on patellofemoral joints (PFJs) remain unclear. We aimed to quantify the changes in the kinematics and cartilage conditions of the PFJ during stair climbing before and after OWHTO.

Controllable Structure Design of an Organic Gel-Infused Porous Surface for Efficient Anti- and De-icing.

Icing causes many problems in daily life and with equipment stability, and many efforts have been made to remove surface icing. Herein, a novel organic gel-infused porous material is developed to achieve excellent de-icing performance. Porous polydimethylsiloxane (P-PDMS) composites with different pore sizes were prepared by a template method.

Isolation, Molecular Characterisation, and Pathogenicity Analysis of a Novel Recombinant ALV-J Strain Isolated From Chinese Hetian Chickens.

Background: Avian leukosis virus subgroup J (ALV-J) primarily affects poultry, particularly chickens, leading to tumourigenesis and immunosuppression, which results in substantial economic losses. It is important to note that ALV-J is commonly found in indigenous chicken breeds in China, and the virus's vertical transmission characteristics present a significant threat to the preservation of local chicken breeds.

Objectives: The study aimed to investigate the characteristics and effects of the recombinant ALV-J strain LY2021J, with a focus on its genetic composition and its potential influence on virulence and pathogenicity.

Correction: Near-infrared light triggered photothermal therapy and enhanced photodynamic therapy with a tumor-targeting hydrogen peroxide shuttle.

Correction for 'Near-infrared light triggered photothermal therapy and enhanced photodynamic therapy with a tumor-targeting hydrogen peroxide shuttle' by Bing Wang , , 2018, , 3145-3155, https://doi.org/10.1039/C8TB00476E.

Gp85 protein encapsulated by alginate-chitosan composite microspheres induced strong immunogenicity against avian leukosis virus in chicken.

Introduction: Avian leukosis, a viral disease affecting birds such as chickens, presents significant challenges in poultry farming due to tumor formation, decreased egg production, and increased mortality. Despite the absence of a commercial vaccine, avian leukosis virus (ALV) infections have been extensively documented, resulting in substantial economic losses in the poultry industry. This study aimed to develop alginate-chitosan composite microspheres loaded with ALV-J Gp85 protein (referred to as aCHP-gp85) as a potential vaccine candidate.

Identification of receptors and factors associated with human coronaviruses in the oral cavity using single-cell RNA sequencing.

Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) ravaged the world, and Coronavirus Disease 2019 (COVID-19) exhibited highly prevalent oral symptoms that had significantly impacted the lives of affected patients. However, the involvement of four human coronavirus (HCoVs), namely SARS-CoV-2, SARS-CoV, MERS-CoV, and HCoV-229E, in oral cavity infections remained poorly understood. We integrated single-cell RNA sequencing (scRNA-seq) data of seven human oral tissues through consistent normalization procedure, including minor salivary gland (MSG), parotid gland (PG), tongue, gingiva, buccal, periodontium and pulp.

Early diagnosis of thyroid-associated ophthalmopathy using label-free Raman spectroscopy and multivariate analysis.

Thyroid-associated ophthalmopathy (TAO) is the most common orbital disease in adults, with complex clinical manifestations and significant impacts on the life quality of patients. The current diagnosis of TAO lacks reliable biomarkers for early and non-invasive screening and detection, easily leading to poor prognosis. Therefore, it is essential to explore new methods for accurately predicting TAO development in its early stage.

Prevalence of natural feline coronavirus infection in domestic cats in Fujian, China.

Only few studies have investigated the prevalence of feline coronavirus (FCoV) infection in domestic cats in Fujian, China. This is the first study to report the prevalence rate of FCoV infection in domestic cats in Fujian, China, and to analyse the epidemiological characteristics of FCoV infection in the region. A total of 112 cat faecal samples were collected from animal hospitals and catteries in the Fujian Province.

Omics analyses of Rehmannia glutinosa dedifferentiated and cambial meristematic cells reveal mechanisms of catalpol and indole alkaloid biosynthesis.

Background: Rehmannia glutinosa is a rich source of terpenoids with a high medicinal reputation. The present study compared dedifferentiated cells (DDCs) and cambial meristematic cells (CMCs) cell cultures of R. glutinosa for terpenoid (catalpol) and indole alkaloid (IA) biosynthesis.