A hydrogel/P(VDF-TrFE) system for enhanced bone regeneration with controllable self-sustained electrical cues.

The limited self-healing capacity of critical-sized bone defects presents significant challenges in healing. An effective approach is to regulate the physicochemical properties of biomaterials to mimic the natural bone regenerative microenvironment. In this work, we have prepared Chitosan-Gelatin (CS-Gel) based hydrogel/ Poly(vinylidene fluoride-co-trifluoroethylene) (P(VDF-TrFE)) systems, which provide biomimetic and electric cues for bone regeneration.

A Novel Self-Regulated, Non-Directional Magnetic Thermos-Brachytherapy I Seed Enhances Anticancer Efficacy by Rescuing Immune Escape.

Integrating mild hyperthermia (MH) with I brachytherapy holds potential for overcoming treatment resistance and improving anticancer efficacy. Here, magnetic nanoparticles (MNPs) with a suitable Curie temperature are constructed and incorporated with silver rods coated with I to form composite seeds. In vitro simulations and in vivo validations demonstrated their effective performance in radiation dose and temperature control.

Research progress on surgical approach and intestinal anastomosis methods for laparoscopic right hemicolectomy: A review.

Patients with tumors located in the cecum, ascending colon, and hepatic flexure of the colon often use laparoscopic right hemicolectomy. Although with the help of laparoscope, the surgery is more visual and refined, due to the different shapes of the blood vessels and the different positions of the frequently occurring blood vessels, the surgeons face challenges during surgery. Improper operation may cause unnecessary damage.

Requirement of CSR-1 isoforms and catalytic activity for lifespan.

The CSR-1 encodes an essential Argonaute protein that binds to 22 nucleotide small guide RNA to regulate germline gene expression. Recent characterization of the two CSR-1 isoforms (a and b) have demonstrated tissue-specific expression and functions. Here, we found that loss of function to the isoform has minimal effect on lifespan while a mutant with deletion to both and isoforms shows a significant decrease in lifespan.

Nonhistone lactylation: A hub for tumour metabolic reprogramming and epigenetic regulation.

Recent research has shifted the perception of lactate from being merely a byproduct of metabolism to being a crucial signalling molecule and epigenetic regulator in tumours. This paper provides a comprehensive review of the mechanisms by which the lactylation of nonhistones contributes to tumorigenesis and tumour development. Lactate accumulates in the tumour microenvironment (TME) due to the Warburg effect and serves as a substrate for the covalent modification of nonhistone proteins (such as p53 and PD-L1) through both enzymatic and nonenzymatic processes.

A novel combination therapy with Uridine and Praziquantel effectively alleviates schistosomiasis-induced hepatic fibrosis through promoting adipogenic differentiation.

Schistosomiasis-induced hepatic fibrosis, a consequence of egg-induced granulomatous lesions, remains untreated by current drugs. Therefore, the development of novel antifibrosis drugs is of paramount importance. Our previous study indicated that aberrant uridine concentrations play a pivotal role in schistosomiasis-induced hepatic fibrosis.

A fitted model for calculating cellular viscoelastic parameters.

The viscoelastic properties of cells reflect their biological behaviors and disease states, and are usually calculated based on atomic force microscope (AFM) indentation curves. But the indentation scenario of cells using AFM probes does not conform to the assumptions of the Hertz model, incurring errors of the calculated values. The existing correction models primarily address small deformation errors, and the failure of the semi-infinite space assumption due to the finite spreading area of cells is largely ignored.

SChLAP1 regulates the metastasis and apoptosis of prostate cancer partly via miR-101.

Background: Prostate cancer (PC) remains one of the leading causes of cancer-related mortality, necessitating further research into novel prognostic biomarkers and therapeutic targets. Long noncoding RNA second chromosome locus-associated with prostate-1 (SChLAP1) plays a crucial role in the aggressiveness of PC; however, its precise mechanism remains unclear. This study aimed to investigate the role of SChLAP1 in PC metastasis and apoptosis, with a particular focus on its interaction with miR-101.

Bidirectional two-sample Mendelian randomization analysis reveals a causal effect of chronic pain on chronic kidney diseases and renal function.

Objective: A growing body of research has shown a connection between chronic pain and chronic kidney disease (CKD). However, it is unclear if these correlations point to a cause-and-effect link. Our goal is to investigate the causal link between renal function or CKD and chronic pain.

Light-Regulated P(VDF-TrFE)/BiS Microneedle Patch with Tunable Electric Stimulation for Modulating the Healing of Burn Wound Dynamically.

As the skin wound forms, external electric stimulation (ES) is required to mimic the endogenous electric field that has been damaged, in order to mediate cellular behavior and promote tissue healing. At the same time, there are certain differences in the external electric stimulation requirements at different stages of skin wound healing. In this study, a Poly(vinylidene fluoride-co-trifluoroethylene)/Bismuth sulfide (P(VDF-TrFE)/Bi₂S₃) microneedle (MN) patch that is able to provide external ES to damaged tissue is developed.

Alleviation of neuroinflammation on electrode interface by biomimetic electrical microenvironment modulation based on collagen/polypyrrole composite film.

Neuroinflammation associated with inflammatory activation of astrocytes on the interface of implanted neural electrodes is the main cause of electrode failure. In this work, collagen/polypyrrole composite film (CPF) was designed and fabricated to modify the interface. The CPF had the co-exposure of collagen and polypyrrole in morphology, which allowed the biomimetic microenvironment provided by collagen to effectively inhibit the inflammatory activation of astrocytes.

Bio-Based Polyurethane Asphalt Binder with Continuous Polymer-Phase Structure: Critical Role of Isocyanate Index in Governing Thermomechanical Performance and Phase Morphology.

Polyurethane asphalt (PUA) has attracted considerable attention in the field of pavement engineering. However, traditional PUA systems typically exhibit low concentrations of polyurethane (PU), leading to a continuous bitumen-dominated phase that adversely affects mechanical properties. Furthermore, the non-renewable nature of raw materials raises environmental concerns.

Discovery of 3-phenyl-1H-5-pyrazolylamides as PLpro inhibitors through virtual screening and structure optimization.

The papain-like protease (PLpro) of SARS-CoV-2 has been identified as a pivotal enzyme in viral replication, indicating it a promising target for drug discovery. Utilizing a virtual screening strategy, compound 1 with the N-(3-(5-amino-1H-pyrazol-3-yl)phenyl) benzenesulfonamide scaffold was discovered as a hit targeting PLpro. Structural modification from virtually screened hit 1 led to the development of a series of substituted 3-phenyl-1H-5-pyrazolylamide derivatives.

WTAP Mediated m6A Modification Stabilizes PDIA3P1 and Promotes Tumor Progression Driven by Histone Lactylation in Esophageal Squamous Cell Carcinoma.

Esophageal squamous cell carcinoma (ESCC) is a common digestive tract malignant cancer with high incidence and mortality rate. Many studies have shown that long noncoding RNAs (lncRNAs are involved in the progression of various types of tumors. The lncRNA protein disulfide isomerase family A member 3 pseudogene 1 (PDIA3P1) promotes the progression of ESCC, but the molecular mechanism behind this is still unclear.

High-content cell imaging for chemical toxicity screening in the model organism Caenorhabditis elegans.

The use of animal models for screening environmental chemicals for toxicity is an important step towards determining potential hazards to humans. Due to the large number of environmental chemicals with unknown biological activity, high-throughput screening has served as the primary method in toxicity testing for the past decades. However, with the emergence of diverse cellular targets that have been shown to be adversely affected by chemicals, a transition towards high-throughput screening that incorporates high-content analysis provides an array of cutting-edge experimental advantages.

Mutant alleles of the Caenorhabditis elegans rde-1 gene identified through chemical mutagenesis of an snRNA misprocessing reporter.

The processing of small nuclear RNA post transcription involves endolytic cleavage of a 3' tail to produce a mature transcript that is incorporated into the spliceosome to regulate RNA splicing. We previously reported in the Caenorhabditis elegans model several novel genetic regulators including those functioning in RNAi processing to be required for snRNA cleavage through a genome-wide RNAi screen using an in vivo snRNA misprocessing reporter. Here, we conducted a forward genetic screen using the mutagen ethyl methanesulfonate to screen for viable mutants that exhibit constitutive snRNA misprocessing.

Hsa_circ_0000479 promotes gastric cancer progression by inhibiting BTRC-mediated ubiquitination of G3BP1.

An increasing number of studies have shown that circular RNAs (circRNAs) are key regulators of cancer development and progression. RNA-binding proteins (RBPs) play critical roles in the regulation of biological activities, such as RNA synthesis, selective splicing, modification, translocation, and translation; therefore, research on the interactions of circRNAs with RBPs is key to identifying potential targets for cancer treatment. However, the biological roles and mechanisms of circRNAs in gastric cancer (GC) remain largely unknown.

Disulfidptosis in tumor progression.

Disulfidptosis, a regulated cell death modality driven by the cystine transporter solute carrier family 7 member 11 (SLC7A11), is characterized by actin cytoskeleton collapse under glucose starvation. This review systematically elucidates the pivotal role of disulfidptosis in tumor metabolic reprogramming, with a focus on its molecular mechanisms and distinctions from other cell death pathways. The core mechanisms include SLC7A11-mediated cystine overload and NRF2/c-Myc-regulated pentose phosphate pathway activation.

Gender Differences in Predicting Metabolic Syndrome Among Hospital Employees Using Machine Learning Models: A Population-Based Study.

Background: Metabolic syndrome (MetS) is a complex condition that captures several markers of dysregulation, including obesity, elevated blood glucose levels, dyslipidemia and hypertension. Using an approach to early prediction of MetS risk in hospital employees that takes into account the differing effects of gender may be expected to improve cardiovascular disease-related health outcomes.

Purpose: In this study, machine learning techniques were applied to construct an optimized MetS prediction model for use on hospital employees.

Multifunctional Double-Loaded Oral Nanoplatform for Computed Tomography Imaging-Guided and Integrated Treatment of Inflammatory Bowel Disease.

Excessive reactive oxygen species, disruption of the epithelial barrier, immune dysregulation, and gut microbiota imbalance are key factors driving the onset of inflammatory bowel disease (IBD) and complicating its treatment. Prompt diagnosis of diseases and precise delivery of therapeutic agents to inflamed intestinal sites offer promising targeted strategies for effectively treating IBD. Here, a barium sulfate-based nanoplatform (BaSO@PDA@CeO/DSP, BPCD) for synergistic delivery of nanozymes and drugs was developed.

m6A-modified LINC02418 induces transcriptional and post-transcriptional modification of CTNNB1 via interacting with YBX1 and IGF2BP1 in colorectal cancer.

Colorectal cancer (CRC) represents a significant menace to human health, but its molecular pathogenesis remains unclear. Herein, we explored the functional role of LINC02418 in CRC progression. The function of LINC02418 in CRC was determined through vitro and in vivo experiments.

Differential effect of ubiquitous and germline depletion of Integrator complex function on C. elegans physiology.

The Integrator is a metazoan-conserved protein complex with endonuclease activity that functions to cleave various RNA substrates to shape transcriptome homeostasis by coordinating small nuclear RNA biogenesis to premature transcription termination. Depletion of Integrator results in developmental defects across different model systems and has emerged as a causative factor in human neurodevelopmental syndromes. Here, we used the model system Caenorhabditis elegans to enable study of the temporal effects of Integrator depletion on various physiological parameters with the auxin-inducible degron system that permitted depletion of INTS-4 (Integrator subunit) catalytic subunit of the protein complex.

Hsa_circ_0002301 inhibits ferroptosis in gastric cancer by encoding the de novo protein HECTD1-463aa.

Background: CircRNAs are closely related to ferroptosis in gastric cancer cells; however, the mechanism by which circRNAs regulate ferroptosis in gastric carcinogenesis remains unknown. CircRNA-encoded novel peptides are functional products translated from the open reading frames (ORFs) within circular RNAs, demonstrating that circRNAs not only serve as non-coding regulators but also have the capacity to encode biologically active peptides. Compared with noncancerous cells, cancer cells have greater iron requirements, and ferroptosis occurs in response to radiotherapy, chemotherapy, and immunotherapy; therefore, ferroptosis activation may be a potential strategy to overcome the shortcomings of conventional cancer therapy.

Clinical comparative study of the modified superior mesenteric artery approach in total laparoscopic radical resection for right colon cancer - a single-center retrospective study.

Background: To explore the safety and feasibility of the modified approach for accessing the superior mesenteric artery (SMA) in total laparoscopic radical resection for right colon cancer.

Methods: This single-center retrospective study included 107 patients who underwent total laparoscopic radical resection of right colon cancer at The First Affiliated Hospital of Wannan Medical College between August 2022 and December 2023. 53 patients were in the modified SMA approach (modified group) and 54 patients were in the traditional SMA approach (control group).

Decoding allosteric landscapes: computational methodologies for enzyme modulation and drug discovery.

Allosteric regulation is a fundamental mechanism in enzyme function, enabling dynamic modulation of activity through ligand binding at sites distal to the active site. Allosteric modulators have gained significant attention due to their unique advantages, including enhanced specificity, reduced off-target effects, and the potential for synergistic interaction with orthosteric agents. However, the inherent complexity of allosteric mechanisms has posed challenges to the systematic discovery and design of allosteric modulators.