Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Nanodiamonds (NDs) with nitrogen-vacancy (NV) centers are emerging as powerful quantum nanosensors (QNs) in biomedical applications due to their exceptional sensitivity. However, achieving optimal diagnostics performance necessitates both high sensitivity and selectivity; especially in practical biomedical settings, it remains challenging for QNs to provide quantitative analyses when multiple analytes are present. Here, we present a biosensing platform that integrates DNA logic gates (DLGs) with spin-based quantum sensing, termed DLG-QN for ultrasensitive and ultraselective diagnostics. Utilizing an AND DLG, both NDs and magnetic beads (MBs) are functionalized with hairpin DNA strands. In the presence of both miRNA-21 and miRNA-155key biomarkers overexpressed in cancerthe hairpin DNAs undergo conformational changes that facilitate DNA-guided self-assembly of NDs and MBs, enriching the target signal. Resonant microwave modulation of ND fluorescence emission allows for high signal-to-noise ratio (SNR) detection by separating the signal from background fluorescence via spin-enhanced analysis. This platform demonstrated ultrasensitive and ultraselective detection of miRNA-21 and miRNA-155 with a limit of detection of 19.8 fM, highlighting its potential as a general biosensing strategy for precision diagnostics involving multiple biomarkers.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12117423 | PMC |
| http://dx.doi.org/10.1021/jacsau.5c00058 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
DNA Logic-Integrated Quantum Nanosensor for MicroRNA Diagnostics.
JACS Au
May 2025
The Institute for Advanced Studies (IAS), Department of Ophthalmology, Zhongnan Hospital of Wuhan University, State Key Laboratory of Metabolism and Regulation in Complex Organisms, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Nanodiamonds (NDs) with nitrogen-vacancy (NV) centers are emerging as powerful quantum nanosensors (QNs) in biomedical applications due to their exceptional sensitivity. However, achieving optimal diagnostics performance necessitates both high sensitivity and selectivity; especially in practical biomedical settings, it remains challenging for QNs to provide quantitative analyses when multiple analytes are present. Here, we present a biosensing platform that integrates DNA logic gates (DLGs) with spin-based quantum sensing, termed DLG-QN for ultrasensitive and ultraselective diagnostics.
View Article and Find Full Text PDFUltrasensitive and ultra-selective room-temperature HS gas sensor based on CuO-loaded InO 2D porous nanosheets.
J Hazard Mater
August 2025
School of Electronics and Information Engineering, Hebei University of Technology, Tianjin Key Laboratory of Electronic Materials and Devices, 5340 Xiping Road, Beichen District, Tianjin 300401, China; Innovation and Research Institute of Hebei University of Technology in Shijiazhuang, Shijiazhuang
Developing a cost-effective sensing material capable of detecting HS with ultra-sensitivity, ultra-selectivity, and low detection limits at room temperature remains highly anticipated. In this paper, two-dimensional (2D) porous InO nanosheets were prepared by a simple solvothermal method, and then CuO was modified on the InO surface by impregnation. The CuO/InO two-dimensional porous structure allows the fabricated sensor to be highly sensitive to HS at room temperature.
View Article and Find Full Text PDFHighly sensitive and specific uranyl ion detection by a fluorescent sensor containing uranyl-specific recognition sites.
Sci Bull (Beijing)
January 2025
State Key Laboratory of Marine Resource Utilization in South China Sea, Collaborative Innovation Center of Marine Science and Technology, Hainan University, Haikou 570228, China. Electronic address:
Article Synopsis
- Uranium pollution poses a significant health and environmental risk, necessitating effective detection methods for uranyl ions, the prevalent form of uranium.
- A new fluorescent sensor, TPE-EDC, was developed using a specific recognition group that becomes less fluorescent in the presence of uranyl ions, indicating successful binding.
- TPE-EDC demonstrates exceptional sensitivity with a detection limit of 69 pmol/L and a rapid response time of 30 seconds, making it a valuable tool for early detection of uranium contamination.
Developing a novel ultraselective and ultrasensitive label-free direct spectrofluorimetric nanobiosensor for direct highly fast field detection of explosive triacetone triperoxide.
Anal Chim Acta
September 2024
Hormozi Laboratory of Chemistry and Biochemistry, 9861334367, Zabol, Iran. Electronic address:
Background: Direct detection of the notorious explosive triacetone triperoxide (TATP) is very difficult because it lacks facile ionization and UV absorbance or fluorescence. Besides, the current indirect methods are time-consuming and need a pre-step for TATP cleavage to hydrogen peroxide. Moreover, they commonly show significant false-positive results in the presence of some camouflage which limits their field applications.
View Article and Find Full Text PDFElectrochemical nano-biosensor based on electrospun indium zinc oxide nanofibers for the determination of complement component 3 protein.
Mikrochim Acta
July 2023
Department of Electrical Engineering, Indian Institute of Technology Hyderabad, Hyderabad, Telangana, 502285, India.
Age-related macular degeneration (AMD) is a progressive chronic neurodegenerative retinal disease leading to vision loss, irreversible blindness, and visual impairment in older adults worldwide. Complement component 3 (C3) protein has been identified as the most predominant biomarker towards early diagnosis of AMD; therefore, there is an utmost requirement for non-invasive detection of C3 protein in the tear fluids of AMD patients. Considering this, we report an insightful electrochemical sensor capable of detecting clinically relevant concentrations ranging from 10 fg/mL to 1 μg/mL using electrospun indium-doped zinc oxide (InZnO) nanofibers as the transducing layer.
View Article and Find Full Text PDF