Optimization of biocatalytic production of sodium gluconate using a dual-enzyme system.

Sodium gluconate has a wide range of applications, including in the fields of construction, textiles, medicine, the chemical industry, and food, so the industrialized production of sodium gluconate is particularly important. However, the preparation process of sodium gluconate is not mature enough, and the production cost is high, which restricts the development of the industry. In this study, the optimization of process conditions for the catalytic production of sodium gluconate from glucose via a dual-enzyme system of glucose oxidase (GOD) and catalase (CAT) was investigated in detail.

Degradation of AFB in edible oil by amphipathic flower-like immobilized self-assembled peptide-based enzyme mimics.

Removing Aflatoxin B (AFB) from food, particularly edible oil detoxification, is a challenge without losing nutrients and flavor components. In this study, enzyme mimics with high-performance for AFB degradation was developed by drawing inspiration from laccase's structure and catalytic mechanism, which used de novo designed peptides as building blocks to self-assemble into supramolecular catalysts with built-in enzyme-like active sites. To further improve ability, a novel amphiphilic organic-inorganic hybrid nanoflower (EmNF-P) was constructed by using Cu as inorganic component and enzyme mimics as organic component, and surface-modifying with amphipathic polymer.

Intracellular self-assembly and metabolite analysis of key enzymes for L-lysine synthesis based on key components of cellulosomes.

Introduction: Cellulosome is a natural multi-enzyme complex in the extracellular space of anaerobic microorganisms, which has the advantages of small molecular weight, multiple binding sites, and strong designability. This study aimed to explore the influence of intracellular self-assembly complexes on L-lysine biosynthesis.

Methods: Two novel L-lysine-engineered bacteria modification strategies were designed, considering the L-lysine biosynthesis pathway using DocA-S3/Coh as an efficient intracellular assembly element: pairwise assembly of key enzymes in cells and multi-enzyme assembly based on scaffolding proteins.

High-throughput screening of Aureobasidium pullulans for high β-glucan production using UV mutagenesis combined with artificial fluorescent labeling.

Aureobasidium pullulans (A. pullulans) is a yeast-like fungus that produces β-glucan, an exopolysaccharide, during its fermentation. However, there is currently a lack of high-throughput screening and breeding methods for high β-glucan-producing A.

Preparation, identification, and anti-photoaging effect of royal jelly protein peptides on UVB-irradiated HaCaT cells.

This study aimed to prepare and identify anti-photoaging peptides from royal jelly proteins (RJP), and to investigate their protective effects and molecular mechanisms against ultraviolet B (UVB)-induced photoaging in human keratinocytes. A low-molecular-weight peptide fraction (<3 kDa, P-RJP1) exhibiting high antioxidant activity was obtained through pepsin hydrolysis of RJP followed by ultrafiltration fractionation. It was found that P-RJP1 effectively restored the viability of UVB-irradiated human keratinocytes, and alleviated oxidative stress by enhancing the activity of antioxidant enzymes and reducing intracellular reactive oxygen species (ROS) levels.

Engineering Compartmentalization and Cofactor Regulation for 10-Hydroxy-2-decenoic Acid Biosynthesis in .

10-Hydroxy-2-decenoic acid (10-HDA), a bioactive component of royal jelly, exhibits significant pharmacological value in various applications. To date, its biosynthesis has been reported exclusively in endotoxin-prone , which limits its biomedical utility. In this study, we successfully engineered the Generally Recognized As Safe (GRAS) yeast as a biosynthetic platform for producing 10-HDA, thereby addressing the limitations associated with systems.

Enzyme mimics based on self-assembled peptide functionalized with graphene oxide for polyethylene terephthalate degradation.

The degradation of polyethylene terephthalate (PET) has garnered notable attention owing to its widespread accumulation and the challenges associated with its breakdown. Herein, the enzyme mimics with PET-hydrolytic activity were developed by combining peptide nanofibers with graphene oxide (GO). Inspired by native enzymes, we designed self-assembled peptides that included active amino acids (serine, histidine, aspartate and tryptophan) and different hydrophobic amino acids, with a 9-fluorenylmethoxycarbonyl group at the N-terminus.

Proline Improves Pullulan Biosynthesis Under High Sugar Stress Condition.

Pullulan is an extracellular polysaccharide produced via the fermentation of . However, high sugar concentrations and hyperosmotic stress limit pullulan biosynthesis during the fermentation process. Therefore, we investigated the effects of proline supplementation on .

Optimization of fermentation conditions for whole cell catalytic synthesis of D-allulose by engineering Escherichia coli.

D-allulose/D-psicose is a significant rare sugar with broad applications in the pharmaceutical, food, and other industries. In this study, we cloned the D-allulose 3-epimerase (DPEase) gene from Arthrobacter globiformis M30, using pET22b as the vector. The recombinant E.

Enhancing the erythritol production of Yarrowia lipolytica by high-throughput screening based on highly sensitive artificial sensor and anchor protein cwp2.

Unlabelled: Yarrowia lipolytica is widely used for the industrial production of the natural sweetener erythritol. Despite improvements in fermentation process control and metabolic pathway regulation, bottlenecks still exist in terms of yield and screening technology. Therefore, we constructed an artificial sensor system for effective erythritol detection, established a single-cell droplet-based high-throughput screening system based on fluorescence-activated cell sorting, and obtained Y.

Understanding Cytochrome P450 Enzyme Substrate Inhibition and Prospects for Elimination Strategies.

Cytochrome P450 (CYP450) enzymes, which are widely distributed and pivotal in various biochemical reactions, catalyze diverse processes such as hydroxylation, epoxidation, dehydrogenation, dealkylation, nitrification, and bond formation. These enzymes have been applied in drug metabolism, antibiotic production, bioremediation, and fine chemical synthesis. Recent research revealed that CYP450 catalytic kinetics deviated from the classic Michaelis-Menten model.

Biochemical and structural characterization of a novel -isoleucine-4-dioxygenase (RaIDO) from Rahnella aquatilis.

The -isoleucine-4-dioxygenase converts -isoleucine (Ile) into(2S,3R,4S)-4-(OH)-isoleucine (4-HIL), a naturally occurring hydroxyl amino acid, which is a promising compound for drug and functional food development. Here, a novel -isoleucine-4-dioxygenase (RaIDO) from Rahnella aquatilis was cloned, expressed and characterized, as one of only a few reported -isoleucine-4-dioxygenases. RaIDO showed high catalytic efficiency with Ile as the substrate, as well as good stability.

The impacts of pullulan soaking on radish seed germination and seedling growth under salt stress.

Pullulan can not only provide a source of organic carbon but also has excellent properties. However, current research is mostly limited to the physical properties of the high-molecular-weight components of pullulan, and little is known about the application of its low-molecular-weight components. This study was designed to explore the impact of presoaking of radish seeds in a pullulan solution on seed germination and subsequent seedling growth under salt stress conditions.

Biosynthesis of 10-Hydroxy-2-decenoic Acid through a One-Step Whole-Cell Catalysis.

10-Hydroxy-2-decenoic acid (10-HDA) is an important component of royal jelly, known for its antimicrobial, anti-inflammatory, blood pressure-lowering, and antiradiation effects. Currently, 10-HDA biosynthesis is limited by the substrate selectivity of acyl-coenzyme A dehydrogenase, which restricts the technique to a two-step process. This study aimed to develop an efficient and simplified method for synthesizing 10-HDA.

Construction and Characterization of Fitting Equations for a New Wheat Straw Pulping Method.

The pretreatment of pulp with enzymes has been extensively studied in the laboratory. However, due to cost constraints, the application of enzymes in the pulp and paper industry is very limited. In this paper, an environment-friendly and efficient pulping method is proposed as an alternative to traditional pulping and papermaking methods.

Development of Raw Materials and Technology for Pulping-A Brief Review.

Paper is one of the most significant inventions in human civilization, which considerably advanced global cultural development. Pulping is a key step in the conversion of fiber raw materials into paper. Since its inception, pulping has rapidly evolved, continually adapting to technological advancements.

A specific and rapid method for detecting and species in Daqu.

Daqu is a spontaneous, solid-state cereal fermentation product used for saccharification and as a starter culture for Chinese Baijiu production. and , two dominant microbial genera in Daqu, produce enzymes and organic acids that influence the Daqu quality. However, there are no rapid analytical methods for detecting and .

Flow-cytometric cell sorting coupled with UV mutagenesis for improving pectin lyase expression.

Alkaline pectin lyase is an important enzyme with a wide range of applications in industrial production, It has been widely used in many important fields such as fruit juice processing and extraction, the dyeing and processing of cotton and linen textiles, degumming plant fibers, environmental industrial wastewater treatment, and pulp and paper production. PGLA-rep4 was previously generated as a modified alkaline pectin lyase with high specific activity at pH 11.0°C and 70°C.

Improvement of optimum pH and specific activity of pectate lyase from RN.1 using loop replacement.

Alkaline pectate lyase plays an important role in papermaking, biological refining and wastewater treatment, but its industrial applications are largely limited owing to its low activity and poor alkali resistance. The alkaline pectate lyase BspPel from RN.1 was heterologously expressed in BL21 (DE3) and its activity and alkali resistance were improved by loop replacement.

Improving the regeneration rate of deep lethal mutant protoplasts by fusion to promote efficient L-lysine fermentation.

Background: L-lysine is widely used for feed and special diet products. The transformation of fermentation strains plays a decisive role in the development of these industries. Based on the mutation breeding theory and metabolic engineering methods, this study aimed to improve the regeneration rate of high-lethality protoplasts by combining multiple mutagenesis and homologous cell fusion techniques to efficiently concentrate multiple dominant mutations and optimize the L-lysine production strain Escherichia coli QDW.

Switchable enzyme mimics based on self-assembled peptides for polyethylene terephthalate degradation.

Polyethylene terephthalate (PET), the most abundant polyester plastic, has become a global concern due to its refractoriness and accumulation in the environment. In this study, inspired by the structure and catalytic mechanism of the native enzyme, peptides, based on supramolecular self-assembly, were developed to construct enzyme mimics for PET degradation, which were achieved by combining the enzymatic active sites of serine, histidine and aspartate with the self-assembling polypeptide MAX. The two designed peptides with differences in hydrophobic residues at two positions exhibited a conformational transition from random coil to β-sheet by changing the pH and temperature, and the catalytic activity followed the self-assembly "switch" with the fibrils formed β-sheet, which could catalyze PET efficiently.

Biosynthesis and genetic engineering of phenazine-1-carboxylic acid in Lzh-T5.

Phenazine-1-carboxylic acid (PCA) is a biologically active substance with the ability to prevent and control crop diseases. It was certified as a pesticide by the Ministry of Agriculture of China in 2011 and was named "Shenzimycin." Lzh-T5 is a strain found in the rhizosphere of tomatoes.

Exploration of microbiome diversity of stacked fermented grains by flow cytometry and cell sorting.

Sauce-flavor baijiu is one of the twelve flavor types of Chinese distilled fermented product. Microbial composition plays a key role in the stacked fermentation of Baijiu, which uses grains as raw materials and produces flavor compounds, however, the active microbial community and its relationship remain unclear. Here, we investigated the total and active microbial communities of stacked fermented grains of sauce-flavored Baijiu using flow cytometry and high-throughput sequencing technology, respectively.

Use of fulvic acid-like compounds from pulp-derived black liquor for enhancing the selenium content of peanut buds.

Background: Cleaner production involving the extraction of useful material from the black liquor by-product of straw pulp would be environmentally beneficial and would permit increased wastewater usage.

Results: The fulvic-acid-like components of pulp black liquor (PFA) with molecular weights below 10 kDa were isolated. The chemical and physiological characteristics of PFAs were investigated.