The impact of paternal body mass index on semen parameters and assisted reproductive outcomes in couples undergoing IVF/ICSI-ET treatment.

Purpose: To investigate the impact of paternal body mass index (BMI) on semen parameters and assisted reproductive outcomes in couples undergoing their first IVF/ICSI-ET cycle.

Materials And Methods: This retrospective study included 7618 couples undergoing IVF/ICSI-ET for maternal tubal/pelvic factors from 2017 to 2023. Paternal BMI was classified according to WHO criteria: normal weight (n = 3433), overweight (n = 3248), and obese (n = 937).

A multicenter, real-world study analysis: pembrolizumab changes treatment strategy for the patient with recurrent/metastatic head and neck squamous cell carcinoma.

Anti-PD-1/PD-L1 has made breakthrough progress in the treatment of recurrent and metastatic head and neck squamous cell carcinoma (R/M HNSCC). However, the Asian population in KEYNOTE-048 only accounts for 13.2% of the total population, and there is a lack of data on the mainland Chinese population.

Effects of Ursolic Acid on Immune Function and Antioxidative Capacity in Weaned Rabbits.

This study aimed to investigate the effects of dietary supplementation with different levels of ursolic acid (UA) on the growth performance, immune function, intestinal antioxidant capacity, and anti-inflammatory responses of weaned rabbits. A total of 160 Hyla meat rabbits aged 35 days were randomly assigned to four groups. Each treatment group consisted of 8 replicates, with 5 rabbits per replicate.

Glioma cells produce soluble PD-L1 via the Wnt/β-catenin signaling pathway to suppress CD8+ T cell activity.

Soluble programmed death ligand 1 (sPD-L1) is emerging as a novel prognostic marker, potentially replacing PD-L1 for assessing prognosis and immunotherapy effectiveness. However, little is known about sPD-L1. This study aimed to assess sPD-L1's potential as a biomarker and explore its origin and biological function.

F-FAPI-04 PET/CT Imaging of Rare Brain Metastasis from Myxoid Pleomorphic Liposarcoma.

This study reports the first case of solitary brain metastasis from myxoid pleomorphic liposarcoma (MPLS) evaluated using F-FAPI-04 PET/CT. The imaging revealed a distinct "Halo Sign," reflecting the infiltration characteristics of cancer-associated fibroblasts (CAFs) within this rare tumor type. These findings highlight the utility of F-FAPI-04 PET/CT in characterizing tumor heterogeneity and underscore its potential to improve the diagnosis of rare brain metastases while informing future therapeutic strategies.

Comparative analysis of cumulative live birth rates in patients with recurrent pregnancy loss undergoing preimplantation genetic testing for aneuploidy versus conventional in vitro fertilisation/intracytoplasmic sperm injection: a retrospective study.

Background: Recurrent pregnancy loss (RPL) affects 1-2% of women worldwide and poses diagnostic and therapeutic challenges due to its multifactorial causes. Preimplantation genetic testing for aneuploidy (PGT-A) aims to improve outcomes by selecting euploid embryos, but its benefits in RPL patients remain uncertain. This study compared the effectiveness of PGT-A versus conventional in vitro fertilisation/intracytoplasmic sperm injection (IVF/ICSI) in improving cumulative live birth rates (CLBRs) and explored the effects of maternal age and miscarriage frequency on treatment efficacy.

High efficiency and a narrow spectrum spectral beam combining a module with a single grating external cavity.

A single grating multiple diffraction spectral beam combining (SBC) structure is proposed and demonstrated, which keeps high efficiency by using the methods of polarization separation and full feedback. The maximum combining efficiency is 81.1%, which leads to an output power of 12.

High beam quality, high brightness dense spectral beam combining of 650 nm laser diode module.

Dense spectral beam combining (DSBC) of a laser diode module at a wavelength of 650 nm was realized for what we believe to be the first time. In this paper, the optimal spectral locking interval range for the 650 nm wavelength band was obtained by stimulation. A DSBC device containing 19 single emitters was designed and built according to the optimal spectral interval.

Polygonal Wrinkles in a Soft Solidified Droplet.

The widespread occurrence of liquid freezing has profound implications for materials science, advanced manufacturing, and the global climate. Emerging from the intricate interplay of fluid dynamics, heat transfer, and phase change, recently, a captivating array of artistic and elegant patterns has been revealed. However, the solidified liquid component generally displays a pronounced resistance to deformation, thereby hindering the critical mechanical coupling inherently associated with thin films and soft materials.

Nitrogen Addition and Warming Drive Multilevel Plant Carbon Allocation via Storage-Growth Trade-Offs: A Meta-Analysis.

Atmospheric nitrogen (N) deposition and global warming are becoming increasingly severe, yet their combined effects on plant carbon allocation, a key factor influencing ecosystem carbon sequestration, remain poorly understood. Through a global meta-analysis (1371 experimental output observations), we investigated how N addition and warming regulated carbon allocation strategies across multiple plant levels and species types. Results revealed that N addition significantly enhanced above-ground nonstructural carbohydrates (NSC) storage, whereas both warming and N addition reduced below-ground NSC reserves.

The change of soluble programmed cell death ligand-1 (sPD-L1): A new predictor of prognosis in hypopharyngeal squamous cell carcinoma patients treated with radiotherapy.

PurposeThe aim of this study was to evaluate the concentration of soluble programmed cell death ligand-1 (sPD-L1) in the plasma of hypopharyngeal squamous cell carcinoma (HSCC) patients before and after radiotherapy (RT) and to explore its correlation with prognosis.MethodsA total of 47 patients and 12 healthy individuals were enrolled. All blood samples were collected before RT and 35 blood samples were obtained after RT.

Real-time monitoring of subcellular HO dynamics by genetically encoded probe roGFP2-PRXIIB.

In plants, genetically encoded probes based on redox-sensitive green fluorescent protein (roGFP) have been used to detect hydrogen peroxide (HO) levels by fusing exogenous thiol peroxidases, such as Orp1 and Tsa2. However, the effectiveness of these thiol peroxidases compared to endogenous ones remains unexplored. Here, we develop a HO probe by fusing roGFP2 to an endogenous HO sensor, type II peroxiredoxin (PRXIIB), which displayed enhanced responsiveness and conversion kinetics compared to roGFP2-Orp1 in vitro and superior sensitivity to HO in vivo.

Evaluation of safety and stability after intraocular lens implantation using a novel material-crosslinked polyisobutylene: A preliminary study.

Purpose: To evaluate the safety and stability of an intraocular lens(IOL) made of crosslinked polyisobutylene (xPIB), a novel biomaterial for new-generation foldable IOL.

Setting: Eye Hospital of Wenzhou Medical University, Hangzhou, Zhejiang Province, China.

Design: Prospective observational study.

Digital innovation in ophthalmic admissions: Impact on hospital quality in China.

Objective: This study aims to construct and evaluate a digital admission management platform for ophthalmology in China, assessing its impact on nurse reception time, patient satisfaction, human cost and healthcare staff experience, with the goal of enhancing the efficiency and quality of ophthalmic medical services.

Methods: A multidisciplinary team conducted a digital transformation, involving needs assessment, process analysis, and design of a digital admission preparation center system tailored for ophthalmic hospitals. We collected data on nursing times for processing admissions and receiving new patients, patient satisfaction ratings, and healthcare staff feedback before and after the implementation of the digital system to evaluate its impact on operational efficiency.

Effect of Meibomian Gland Expression on Optical Quality in Patients with Meibomian Gland Dysfunction.

Purpose: To evaluate the changes in meibomian gland function, ocular surface parameters, and optical quality before and after meibomian gland expression in patients with meibomian gland dysfunction (MGD).

Methods: Patients with MGD underwent meibomian gland expression once weekly for 4 consecutive weeks. Comprehensive assessments, including the Ocular Surface Disease Index questionnaire, tear break-up time, corneal fluorescein staining, lid margin abnormality grading, meibum expressibility and quality evaluations, Schirmer I test (SIT), as well as optical quality examinations using the Pentacam and Optical Quality Analysis System II, were conducted before and after the treatment.

Peptide-based immuno-PET/CT monitoring of dynamic PD-L1 expression during glioblastoma radiotherapy.

Real-time, noninvasive programmed death-ligand 1 (PD-L1) testing using molecular imaging has enhanced our understanding of the immune environments of neoplasms and has served as a guide for immunotherapy. However, the utilization of radiotracers in the imaging of human brain tumors using positron emission tomography/computed tomography (PET/CT) remains limited. This investigation involved the synthesis of [F]AlF-NOTA-PCP2, which is a novel peptide-based radiolabeled tracer that targets PD-L1, and evaluated its imaging capabilities in orthotopic glioblastoma (GBM) models.

3D Periodic Orbiting of a Photothermal Bubble.

The spontaneous periodic oscillations of underwater bubbles are typically confined to a 1D direction or within a 2D plane, whereas realizing 3D autonomous motion of bubbles remains challenging. In this Letter, we present experimental observations of a 3D-periodic orbiting bubble, produced by directing a stationary near-infrared laser at hexane, thereby extending the spectrum of multimode motions beyond the 1D and 2D paradigms. Through numerical simulation by carefully investigating both the thermodynamic and hydrodynamic parameters of several liquids, this 3D-orbiting mode is attributed to the subtle interplay between the interfacial flow due to the thermal Marangoni effect and the bulk flow from the thermal buoyancy effect.

Response mechanisms of eukaryotic plankton community structure to complex environmental conditions in semi-arid river basins, China.

Semi-arid regions, such as the Yellow River Basin in China, are highly sensitive to environmental changes due to their scarce water resources and fragile ecosystems. This study explores the response mechanisms of eukaryotic plankton community structure to environmental conditions in the Dahei River Basin, a representative semi-arid region in central-western Inner Mongolia, across different seasons (spring, summer, autumn) and regions (hills, plain, urban). Our results reveal significant temporal and spatial variability in water resources and quality factors, which in turn affect eukaryotic plankton community structure.

Subcellular proteomics reveals the crosstalk between nucleocytoplasmic trafficking and the innate immune response to Senecavirus A infection.

Mounting evidence suggests that a number of host nuclear-resident proteins are indispensable for the replication of picornaviruses, a typical class of cytoplasmic RNA viruses. Host nucleocytoplasmic transport is often hijacked by viruses to promote their replication in the cytoplasm of infected cells, and suppress the innate immune response. However, little is known about the mechanisms by which Senecavirus A (SVA) manipulates nucleocytoplasmic trafficking events to promote infection.

Peptide-based PET/CT imaging visualizes PD-L1-driven radioresistance in glioblastoma.

Radioresistance remains a great challenge for radiotherapy in the treatment of glioblastoma (GBM). PD-L1 expression is a key contributor to radioresistance and immune escape in GBM. The lack of effective methods to monitor the change of PD-L1 during radiotherapy in patients limits timely intervention and management of the resistance.

Unilateral Exudative Retinal Detachment and Uveitis Accompanied by a Large Subretinal Deposit on the Macula Secondary to Bartonella Henselae Infection.

Bartonella henselae is a Gram-negative bacillus, mainly parasitizing on cats. When a child is scratched by a cat, they may present with the disease symptoms including regional lymphadenopathy, malaise, fever, and splenomegaly, which is known as cat-scratch disease (CSD). Ocular manifestations occur in 5-10% of patients with CSD.

A hovering bubble with a spontaneous horizontal oscillation.

Active matters, characterized by multi-mode motions, have been emerging for both engineering and biological applications. Generally, active objects rely on the symmetry-broken structures, compositions, or interfacial activities through a physical or chemical approach. Here, we report an active bubble spontaneously hovering with a horizontal oscillation at the solid/liquid interface by impacting a stationary laser beam into a liquid through a transparent solid cover.

TiO2-Nanoparticle-Enhanced Sonodynamic Therapy for Prevention of Posterior Capsular Opacification and Ferroptosis Exploration of Its Mechanism.

Purpose: To explore the application and potential ferroptosis mechanisms of sonodynamic therapy (SDT) using titanium dioxide nanoparticles (TiO2-NPs) as sonosensitizers for the prevention of posterior capsule opacification (PCO).

Methods: We fabricated TiO2-NP-coated intraocular lenses (TiO2-IOLs) using the spin-coating method, followed by ultrasound activation of the photosensitizer TiO2. In vitro experiments were performed with human lens epithelial cells (HLECs) to explore the appropriate concentration of TiO2 and ultrasonic parameters.

Homozygous mutation of KISS1 receptor () gene identified in a Chinese patient with congenital hypogonadotropic hypogonadism (CHH): case report and literature review.

Objectives: Congenital hypogonadotropic hypogonadism (CHH) is a rare condition caused by a defect in the production, secretion or action of gonadotropin-releasing hormone. The absence of puberty and varying degrees of gonadotropic deficiency are common symptoms of this disorder. Heterogeneity exists in the clinical presentation of the different clinical subtypes and multiple genes have been implicated in CHH.

Identification and Characterization of a Novel B Cell Epitope of ASFV Virulence Protein B125R Monoclonal Antibody.

The African swine fever virus (ASFV) is an ancient, structurally complex, double-stranded DNA virus that causes African swine fever. Since its discovery in Kenya and Africa in 1921, no effective vaccine or antiviral strategy has been developed. Therefore, the selection of more suitable vaccines or antiviral targets is the top priority to solve the African swine fever virus problem.