Overexpression of SiGSTU24 enhances salt tolerance in transgenic Arabidopsis.

Background: Soil salinization can lead to reduced soil activity, damage to the plant root system, stunted crop growth and reduced yield. Foxtail millet, an important cereal crop, has high nutritional and economic value but is affected by salt stress during growth. During long-term evolution, foxtail millet has developed various regulatory mechanisms to cope with salt stress.

The Progression of NUS1-Associated Parkinson's Disease and the Diagnostic Potential of Plasma NgBR.

Aims: The study aimed to investigate the role of NUS1 variants in Parkinson's disease (PD) progression and evaluate plasma Nogo-B receptor (NgBR) as a potential biomarker.

Methods: We recruited 228 PD patients, including 38 with NUS1 variants (NUS1-PD) and 190 without (GU-PD), and all underwent at least two follow-up visits. Linear mixed-effects models assessed motor and non-motor symptom progression.

Covalently bonded graphene oxide-carbon nanotube hybrid nanofillers for achieving high-performance polyamide 6 composites with superior mechanical properties and thermal conductivity.

The rise of hydrogen energy places stringent demands on the mechanical strength and thermal conductivity of polyamide 6 (PA6) liners used in hydrogen storage tanks. However, the presence of weak interfaces in PA6 composites significantly hinders the efficient transfer of the intrinsic mechanical and thermal conductivity properties of the reinforcing phases. In this work, hydroxyl-functionalized carbon nanotubes (CNT-OH) were covalently grafted onto graphene oxide (GO) to construct a hybrid nanofiller, which was subsequently surface-functionalized with hexamethylene diisocyanate (HDI), yielding a reactive nanofiller (fGO + CNT-OH + HDI) rich in isocyanate groups.

Precision prognostication in neuroblastomas via clinically validated E2F activity signatures.

Background: Neuroblastoma (NB) is the most common extracranial solid tumor in children, with high-risk NB (HR-NB) exhibiting dismal survival rates due to aggressive biology and therapy resistance. E2F transcription factors (E2Fs) are pivotal regulators of cell cycle progression and immune modulation, yet their prognostic and therapeutic implications in NB remain underexplored.

Methods: Using transcriptomic data from the GEO, TARGET, and E-MTAB-8248 cohorts, we identified E2F-associated molecular subtypes via consensus clustering.

Metabolomics analysis reveal differences in the chemical components of bee bread from and .

Although mature bee bread is a key natural bee product, comprehensive metabolite profiles remain incomplete. This study compared bee bread metabolomes from (BAM) and (BAC) using LC-MS broad-target metabolomics. We identified 1090 metabolites across 19 compound classes, with 269 differentially expressed between species.

Tracheobronchial ossification in children: a case report and review of the literature.

Objective: This study aimed to report a case of tracheobronchial ossification (TO) in a child and review the related literature on its clinical signs, diagnosis, differential diagnosis, pathological characteristics, points, and treatment measures.

Methods: A retrospective analysis was conducted on the clinical data of one pediatric patient with TO admitted to the Department of Pediatrics, Fuzhou Pulmonary Hospital. In addition, three relevant articles published up to July 2024 were reviewed from both domestic and international databases, including PubMed, China National Knowledge Infrastructure (CNKI), Wanfang Data, Chinese Biomedical Literature Database (CBM), Web of Science, and the Cochrane Library.

A Mendelian randomization study on association of alpha-synuclein and GPNMB with Parkinson's disease risk and progression.

The prevalence of Parkinson's disease (PD) is increasing due to the aging population. Early diagnosis and prognosis of PD remain challenging, suggesting that seeking appropriate biomarkers for PD is crucial. Glycoprotein nonmetastatic melanoma protein B (GPNMB) and alpha-synuclein (α-syn) have been reported to contribute to PD pathogenesis and are correlated with PD onset and disease progression.

FEN1-assisted LAMP for specific and multiplex detection of pathogens associated with community-acquired pneumonia.

Lower respiratory tract infections (LRITs), including community-acquired pneumonia (CAP), are the fifth leading cause of death worldwide over the last ten years, posing a serious threat to global healthcare. Conventional laboratory assays for detecting pathogens are hindered by complicated procedures, a long turnaround time and a lack of multiplex detection capabilities. In this study, a flap-endonuclease 1 (FEN1)-assisted loop-mediated isothermal amplification (LAMP) method was designed, and an assay based on this method was developed to identify three leading pathogens for CAP, namely, , and .

Single-cell profiling reveals a reduced epithelial defense system, decreased immune responses and the immune regulatory roles of different fibroblast subpopulations in chronic atrophic gastritis.

Purpose: To identify key cellular changes and molecular events in atrophic mucosa, we aimed to elucidate the molecular mechanisms driving the occurrence of chronic atrophic gastritis (CAG).

Methods: We used single-cell RNA sequencing (scRNA-seq) to characterize changes in the epithelial state and tissue microenvironment associated with CAG. The molecular changes were identified by comparing differentially expressed genes (DEGs) between the two mucosa states.

CircZMYM2 plays a pivotal role in osteosarcoma by regulating the translation of and .

Osteosarcoma (OS) is a primary malignant bone tumor in children and young adults because of its intertumoral heterogeneity and absence of molecular targets. This study aimed to elucidate the role of circular RNA in the pathogenesis of OS. Using bioinformatics analysis and OS clinical samples, we found that hsa_circ_0029634 formed during the splicing process of (circZMYM2) was highly expressed in OS tissues.

Serum metabolic profiles in quails: Effects of age, breed, and sex.

Over the past decade, metabolomic research in livestock and poultry has gained considerable momentum; however, quail metabolomics still lags behind that of livestock species such as chickens, pigs, and cattle. Quails are important models due to their low-cost protein sources-both eggs and meat-and practical benefits such as minimal space requirements, high egg production, disease resistance, and rapid reproduction. Therefore, it is necessary to systematically understand the effects of various factors on quail metabolism to provide a theoretical basis for accurate feeding and breeding practices.

Effect of Compound Fertilizer on Foxtail Millet Productivity and Soil Environment.

The effects of balanced fertilization with nitrogen, phosphorus, and potassium (NPK) on foxtail millet productivity and the soil environment under the same conditions of total nutrients have received limited research attention. Therefore, in this study, three balanced fertilization patterns of 27-14-10 (T1), 27-17-7 (T2), and 30-10-11 (T3), and one no fertilization treatment (CK), a total of four treatments, were set up through a two-year field experiment to study the effects of balanced fertilization patterns on foxtail millet yield and soil environment. Mantel analysis was conducted to reveal the correlation between soil environmental factors and the community and their contribution to productivity.

Cationic cellulose dispersed Ag NCs/C-CNF paper-based SERS substrate with high homogeneity for creatinine and uric acid detection.

To overcome the problems of easy aggregation, poor reproducibility and homogeneity of metal nanosols, a SERS substrate with good sensitivity, homogeneity and reproducibility was designed and prepared for the detection of disease markers in urine. Silver nanocubes (Ag NCs) were firstly prepared and then dispersed in cationic cellulose (C-CNF) to form a homogeneous gel, which was dropped on a filter paper to develop a substrate with good SERS activity. This substrate combines the superior SERS properties of Ag NCs with the stability of C-CNF and has a minimum detection concentration of 10 M for R6G.

Comparative study of robotic-assisted single-incision-plus-one port and single-incision laparoscopic choledochal cyst excision.

Objective: To compare the efficacy of robotic-assisted single-incision-plus-one-port laparoscopic choledochal cyst excision (R-SILC + 1) and single-incision laparoscopic choledochal cyst (SILC) in treating pediatric choledochal cyst (CDC).

Methods: We retrospectively analyzed the clinical data of patients diagnosed with CDC in our hospital from June 2021 to October 2023. Among them, patients underwent either R-SILC + 1 or SILC procedures.

Umbilical double-port laparoscopy combined with extraperitoneal water injection for the treatment of giant inguinal hernias in infants and young children.

Objective: Exploration of the efficacy of treating large indirect inguinal hernias in infants and young children using umbilical double-port laparoscopy combined with extraperitoneal water injection.

Methods: A retrospective analysis was conducted on 165 cases of primary unilateral large indirect inguinal hernias in infants and young children treated at our hospital from May 2018 to May 2023. Among them, 90 cases underwent umbilical double-port laparoscopic surgery combined with extraperitoneal water injection and high ligation of the hernia sac (Double-Port Group), and another 75 cases underwent conventional three-port laparoscopic high ligation of the hernia sac (Three-Port Group).

Construction of nomogram based on clinical factors for the risk prediction of postoperative complications in children with choledochal cyst.

Objective: The aim of the study was to develop a prediction nomogram based on clinical factors to assess the risk of postoperative complications in children with congenital choledochal cyst.

Methods: The clinical data from 131 children who underwent choledochal cyst resection and Roux-en-Y hepaticojejunostomy in our hospital between January 2016 and December 2022 were retrospectively analyzed. The general information, clinical symptoms, procedure, biochemical indicators, and imaging data were recorded.

A trans-umbilical single-site plus one robotic-assisted surgery for choledochal cyst resection in children.

Objective: The purpose of this study is to compare the intraoperative and postoperative outcomes of a trans-umbilical single-site plus one robot-assisted surgery and a trans-umbilical single-site laparoscopic surgery in the treatment of choledochal cysts.

Methods: We retrospectively analyzed clinical data from 49 children diagnosed with choledochal cysts who were admitted to our hospital between June 2020 and December 2023. Among these patients, 24 underwent a trans-umbilical single-site plus one Da Vinci robot-assisted surgery (the robot group) and 25 underwent a trans-umbilical single-site laparoscopic-assisted surgery (the laparoscopic group).

Integrated Analysis of scRNA-Seq and Bulk RNA-Seq Reveals Metabolic Reprogramming of Liver Cancer and Establishes a Prognostic Risk Model.

Liver cancer manifests as a profoundly heterogeneous malignancy, posing significant challenges in terms of both therapeutic intervention and prognostic evaluation. Given that the liver is the largest metabolic organ, a prognostic risk model grounded in single-cell transcriptome analysis and a metabolic perspective can facilitate precise prevention and treatment strategies for liver cancer. Hence, we identified 11 cell types in a scRNA-seq profile comprising 105,829 cells and found that the metabolic activity of malignant cells increased significantly.

Case report: Systemic presentation of ALK-positive Histiocytosis.

ALK-positive Histiocytosis (ALK-HSs) is a recently identified rare clinical entity characterized by tissue histiocytic alterations associated with ALK gene rearrangement. Clinical presentations can be solitary, multifocal, or systemic (involving multiple sites and organs). Due to limited reported cases, there is inadequate understanding of this disease.