Covalently bonded graphene oxide-carbon nanotube hybrid nanofillers for achieving high-performance polyamide 6 composites with superior mechanical properties and thermal conductivity.

The rise of hydrogen energy places stringent demands on the mechanical strength and thermal conductivity of polyamide 6 (PA6) liners used in hydrogen storage tanks. However, the presence of weak interfaces in PA6 composites significantly hinders the efficient transfer of the intrinsic mechanical and thermal conductivity properties of the reinforcing phases. In this work, hydroxyl-functionalized carbon nanotubes (CNT-OH) were covalently grafted onto graphene oxide (GO) to construct a hybrid nanofiller, which was subsequently surface-functionalized with hexamethylene diisocyanate (HDI), yielding a reactive nanofiller (fGO + CNT-OH + HDI) rich in isocyanate groups.

The role of the gut microbiota and its metabolites: a new predictor in diabetes and its complications.

Type 2 diabetes (T2D) is easy to trigger many organ or system lesions, which can lead to various metabolic diseases, such as diabetic kidney disease (DKD), diabetic liver disease, diabetic cardiovascular disease, diabetic foot, etc. Due to the easy availability of stool and blood samples from patients, the study of gut microbes and their metabolites are progressing rapidly. The relationship between pathophysiological alterations of metabolic disorders and gut microbiota composition provides new approaches to precisely identify disease dynamics and refine disease treatment strategies.

Near-Infrared Imaging of Localized Longitudinal Nanoparticle Transport in Living Animals.

Visualizing the in vivo dynamic transport behavior of substances following acupoint injection has remained a significant challenge due to the limitations of conventional imaging techniques. Near-infrared (NIR) imaging, with its superior tissue penetration, reduced autofluorescence, and real-time tracking capability, presents a promising approach for acupoint-associated imaging; however, its application in this context is yet to be experimentally validated. Here, NIR imaging is utilized to uncover highly localized, longitudinal transport of nanoparticles on the body surface in an acupoint-injection mouse model.

Eliciting Dual-Niche Immunological Priming by Acupoint Delivery of Nanovaccines.

Immunization has long played essential roles in preventing diseases. However, the desire for precision delivery of vaccines to boost a robust immune response remains largely unmet. Here, we describe the use of acupoint delivery of nanovaccines (ADN) to elicit dual-niche immunological priming.

Molecular and cellular morphology of placenta unveils new mechanisms of reproductive immunology.

Introduction: Despite of numerous studies of the placenta, some molecular and cellular characteristics, particularly the relationship among different cell types, have not been well understood. We aim to investigate the basic and intricate details of cellular and molecular elements in early and late phase placentas to gain better understanding of the immune regulation of human reproductive process.

Methods: A novel combination of techniques of spatial transcriptomics(ST), multiple immunohistochemistry, and a dual labeling combining immunohistochemistry and (fluorescence in situ hybridization) FISH on normal and ectopic pregnancy and animal models was employed to investigate the placenta at tissue, cell, protein and molecular levels and to trace the fetal and maternal origin of every cell in early and late placentas.

USP7 promotes cardiometabolic disorders and mitochondrial homeostasis dysfunction in diabetic mice via stabilizing PGC1β.

Diabetic cardiomyopathy (DCM) is a major complication of diabetes and is characterized by left ventricular dysfunction. Currently, there is a lack of effective treatments for DCM. Ubiquitin-specific protease 7 (USP7) plays a key role in various diseases.

USP7 cardiomyocyte specific knockout causes disordered mitochondrial biogenesis and dynamics and early neonatal lethality in mice.

Background: Ubiquitination is an enzymatic modification involving ubiquitin chains, that can be reversed by deubiquitination (DUB) enzymes. Ubiquitin-specific protease 7 (USP7), which is also known as herpes virus-associated ubiquitin-specific protease (HAUSP), has been shown to play a vital role in cardiovascular diseases. However, the underlying molecular mechanism by which USP7 regulates cardiomyocyte function has not been reported.

A Comprehensive Analysis of the Peanut SQUAMOSA Promoter Binding Protein-like Gene Family and How Enhances Salt Tolerance in Transgenic .

SPL (SQUAMOSA promoter binding protein-like), as one family of plant transcription factors, plays an important function in plant growth and development and in response to environmental stresses. Despite gene families having been identified in various plant species, the understanding of this gene family in peanuts remains insufficient. In this study, thirty-eight genes (-) were identified and classified into seven groups based on a phylogenetic analysis.

Hyperthermophilic pretreatment composting can reduce ammonia emissions by controlling proteolytic bacterial community and the physicochemical properties.

Proteolysis is the rate-limiting step in the mineralization of organic nitrogen into ammonium (NH) and thereby the ammonia (NH) released during the composting. However, the dynamics of bacterial proteolytic communities related to NH emissions during the composting systems are mostly unknown. This study aimed to examine and compare the effects of hyperthermophilic pretreatment composting (HPC) and traditional composting (TC) methods on (i) the difference of NH loss and nitrogenous compounds; (ii) the dynamics of the proteolytic bacterial community involved in the proteolysis and (iii) the correlation between the proteolytic bacterial community, biophysiochemical characteristics and NH loss.

Semaglutide attenuates pathological electrophysiological remodeling in diabetic cardiomyopathy via restoring Cx43 expression.

Background: Semaglutide is a relatively new anti-hyperglycemic agent that was shown to carry cardioprotective potentials. However, the exact effects of semaglutide on diabetic cardiomyopathy (DCM) and their underlining mechanism remain unclear. This study aimed to evaluate the effects of semaglutide on myocardium injury and cardiac function in DCM mice and its potential mechanisms, with emphasis on its effects on Cx43 and electrophysiological remodeling.

Fufang Zhenzhu Tiaozhi (FTZ) suppression of macrophage pyroptosis: Key to stabilizing rupture-prone plaques.

Background: Research on the Chinese herbal formula Fufang Zhenzhu Tiaozhi (FTZ) has demonstrated its effectiveness in treating hyperlipidemia and glycolipid metabolic disorders. Additionally, FTZ has shown inhibitory effects on oxidative stress, regulation of lipid metabolism, and reduction of inflammation in these conditions. However, the precise mechanisms through which FTZ modulates macrophage function in atherosclerosis remain incompletely understood.

Gasdermin D-Mediated Pyroptosis in Diabetic Cardiomyopathy: Molecular Mechanisms and Pharmacological Implications.

Diabetic cardiomyopathy (DCM) is a pathophysiological condition triggered by diabetes mellitus (DM), which can lead to heart failure (HF). One of the most important cellular processes associated with DCM is the death of cardiomyocytes. Gasdermin D (GSDMD) plays a key role in mediating pyroptosis, a type of programmed cell death closely associated with inflammasome activation.

Rice husk hydrochar prepared by hydrochloric acid assisted hydrothermal carbonization for levofloxacin removal in bioretention columns.

Hydrochars are promising adsorbents in pollutant removal for water treatment. Herein, hydrochloric acid (HCl) co-hydrothermally treated hydrochars were prepared from rice husk biomass at 180 °C via a one-step hydrothermal method. Adsorption behaviors of levofloxacin (LVX) on hydrochars were evaluated.

Phosphorylation of RasGRP1 by Shc3 prevents RasGRP1 degradation and contributes to Ras/c-Jun activation in hepatocellular carcinoma.

Ras guanine nucleotide-releasing protein 1 (RasGRP1), a Ras activator, is upregulated in hepatocellular carcinoma (HCC) and other kinds of cancer and is associated with the poor prognosis of patients. However, little is known about the underlying regulatory mechanisms of RasGRP1 in the context of cancer. Here, we report that RasGRP1 physically interacted with the adaptor protein Src homolog and collagen homolog 3 (Shc3).

Fufang Zhenzhu Tiaozhi (FTZ) capsule ameliorates diabetes-accelerated atherosclerosis via suppressing YTHDF2-mediated mA modification of SIRT3 mRNA.

Ethnopharmacological Relevance: Fufang Zhenzhu TiaoZhi (FTZ), a Chinese medicinal decoction, has continuously been used to treat metabolic syndrome. Atherosclerosis is the main pathological basis of cardiovascular disease. The N6 methyladenosine (mA) modification is a highly dynamic and reversible process involving a variety of important biological processes.

The Chinese herbal medicine Fufang Zhenzhu Tiaozhi ameliorates diabetic cardiomyopathy by regulating cardiac abnormal lipid metabolism and mitochondrial dynamics in diabetic mice.

Diabetic cardiomyopathy (DCM) is an important complication leading to the death of patients with diabetes, but there is no effective strategy for clinical treatments. Fufang Zhenzhu Tiaozhi (FTZ) is a patent medicine that is a traditional Chinese medicine compound preparation with comprehensive effects for the prevention and treatment of glycolipid metabolic diseases under the guidance of "modulating liver, starting pivot and cleaning turbidity". FTZ was proposed by Professor Guo Jiao and is used for the clinical treatment of hyperlipidemia.

The mA Reader YTHDF1 Promotes Lung Carcinoma Progression via Regulating Ferritin Mediate Ferroptosis in an mA-Dependent Manner.

N6-methyladenosine (mA) plays a significant role as an epigenetic mechanism, which is involved in various cancers' progress via regulating mRNA modification. As a crucial mA "reader", YTHDF1 is able to alter mA-modified mRNA and promote the protein translation process in multiple cancers. However, the role of YTHDF1 in lung cancer has not been fully investigated.

3D-Autologous Culture Method for Precision Oncology.

The 3D-autologous culture method (3D-ACM) for patient-derived cancer samples utilizes a patient's own body fluid or serum to prepare a 3D scaffold and for the culture medium. 3D-ACM enables tumor cells and/or tissues from an individual patient to proliferate in vitro, in a microenvironment that is very similar to their original, in vivo surroundings. The purpose is to maximally preserve in culture the native biological properties of a tumor.

Identification of a nucleotide metabolism-related signature to predict prognosis and guide patient care in hepatocellular carcinoma.

Hepatocellular carcinoma is a highly malignant tumor with significant heterogeneity. Metabolic reprogramming plays an essential role in the progression of hepatocellular carcinoma. Among them, nucleotide metabolism needs further investigation.

Fu Fang Zhen Zhu Tiao Zhi Capsules Protect against Myocardial Ischemia by Inhibiting Cardiomyocyte Pyroptosis.

Background: Fu Fang Zhen Zhu Tiao Zhi (FTZ) is a traditional Chinese herbal prescription widely used to treat dyslipidemia, metabolic diseases, and diabetic coronary disorders. Cardiomyocyte death and loss of regenerative ability cause cardiac dysfunction and heart failure. FTZ can effectively treat diabetic cardiomyopathy and macrovascular diseases; however, the mechanism behind the phenomenon is still unclear.

Isolation, Identification and Characterization of Growth Parameters of HSM-C2 with Coumarin-Degrading Bacteria.

Natural coumarins contribute to the aroma of licorice, and they are often used as a flavoring and stabilizing agents. However, coumarins usage in food has been banned by various countries due to its toxic effect. In this study, a strain of HSM-C2 that can biodegrade coumarin with high efficiency was isolated from soil and identified as through performing 16S rDNA sequence analysis.

Compare the performance of multiple machine learning models in predicting tacrolimus concentration for infant patients with living donor liver transplantation.

Background: This study aims to establish multiple ML models and compare their performance in predicting tacrolimus concentration for infant patients who received LDLT within 3 months after transplantation.

Methods: Retrospectively collected basic information and relevant biochemical indicators of included infant patients. CMIA was used to determine tacrolimus C .

BAG3 Alleviates Atherosclerosis by Inhibiting Endothelial-to-Mesenchymal Transition via Autophagy Activation.

Atherosclerosis is a chronic systemic inflammatory disease that causes severe cardiovascular events. B cell lymphoma 2-associated athanogene (BAG3) was proven to participate in the regulation of tumor angiogenesis, neurodegenerative diseases, and cardiac diseases, but its role in atherosclerosis remains unclear. Here, we aim to investigate the role of BAG3 in atherosclerosis and elucidate the potential molecular mechanism.

The Traditional Chinese Medicine Formula FTZ Protects against Cardiac Fibrosis by Suppressing the TGF1-Smad2/3 Pathway.

Background: Fu fang Zhen Zhu Tiao Zhi (FTZ) is a patented preparation of Chinese herbal medicine that has been used as a natural medicine to treat several chronic diseases including cardiovascular disease. However, its effects on cardiac fibrosis remain unclear. Therefore, this study was designed to investigate the effects and potential mechanisms of FTZ in treating cardiac fibrosis.