Integrating Single-Cell and Bulk RNA Sequencing Reveals the Malignant Phenotype of CBX4 in Prostate Cancer.

The expression pattern and functions of CBX4 in prostate cancers remain ambiguous. This study aims to investigate the performance of CBX4 in prostate cancer progression and preliminary inquiry potential mechanisms. The GEPIA data website was utilized to evaluate the expression patterns of CBX families and their correlations with prognosis.

 (Berberidaceae), a new riparian shrub from northern Sichuan, China.

is herein described as a new deciduous species endemic to riparian habitats in Jiuzhaigou, northern Sichuan, China. Phylogenetic analyses based on complete plastome sequences and 322 nuclear loci consistently recover as a distinct and divergent lineage, genetically separated from all morphologically similar species and most closely related to . Species delimitation analyses employing both topology-based (SODA) and substitution-based (bPTP, mPTP) frameworks further corroborate its taxonomic distinctiveness.

Combined clinical and MRI-based radiomics model for predicting acute hematologic toxicity in gynecologic cancer radiotherapy.

Acute hematologic toxicity (HT) remains a critical dose-limiting complication in gynecologic cancer patients undergoing pelvic radiotherapy, particularly when combined with chemotherapy. Early prediction of severe HT could inform personalized management and minimize toxicity. We developed and validated a predictive model integrating clinical parameters and radiomic features, evaluating five machine learning approaches.

The function of GPCRs in different bone cells.

G protein-coupled receptors (GPCRs) are recognized as critical therapeutic targets in bone disorders, owing to their multifaceted regulatory roles across diverse bone cell lineages. This review systematically catalogs GPCR expression and functional heterogeneity in key bone cells: 12 GPCRs in mesenchymal stem cells (MSCs) orchestrate lineage specification; 21 GPCRs in osteoblasts/osteocytes mediate matrix mineralization and mechanotransduction; 23 GPCRs in macrophages/osteoclasts regulate inflammatory bone resorption; 31 GPCRs in chondrocytes govern endochondral ossification and osteoarthritis pathogenesis; and 8 GPCRs in other cell types modulate bone-related physiological processes. By integrating canonical signaling axes-cAMP/PKA-dependent transcriptional networks, PLC-β/IP3-driven calcium signaling, and NF-κB-modulated immuno-skeletal interactions-we elucidate how GPCRs dynamically coordinate cellular plasticity to maintain skeletal homeostasis.

Highly regio- and diastereoselective construction of spirocyclic compounds palladium-catalyzed [3 + 2] cycloaddition of vinyl methylene cyclic carbonates with pyrrolidinones.

A palladium-catalyzed [3 + 2] cycloaddition reaction of vinyl methylene cyclic carbonates with pyrrolidinones was developed. This protocol demonstrates remarkable versatility, enabling the facile construction of diverse [5,5]-spirolactam scaffolds in excellent yields with outstanding diastereoselectivity. The synthetic utility of this protocol was further demonstrated through productive transformations of the resulting spirolactam products, highlighting their potential as valuable intermediates for complex molecule synthesis.

Matrix metalloproteinase-2 and pH-responsive drug eluting multilayer as intraocular lens coating to improve the posterior capsule opacification inhibition.

Intraocular lens (IOL) is a crucial implant for cataract therapy. Posterior capsule opacification (PCO) is the most common postoperative complication after IOL implantation, which is the abnormal hyperplasia of the residual lens epithelial cells (LECs) after IOL implantation in cataract surgery. It is reported that the cellular microenvironment in the lens capsule changes after surgery, such as the elevated secretion of matrix metalloproteinases (MMPs) and a decrease in pH due to undesired cell proliferation.

Imprisoning 2H intermediate phases in blade-coated wide-bandgap perovskites for efficient all-perovskite tandem solar cells.

Scalable fabrication of high-efficiency all-perovskite tandem solar cells (TSCs) remains challenging due to notable voltage deficits in wide-bandgap perovskite solar cells, primarily driven by severe halide segregation during the large-scale blade coating process. Here, we introduce 4-aminobenzylphosphonic acid as a functional "2H-imprison" additive that selectively bypasses the formation of the 2H phase (an iodine-rich structure) and promotes the direct crystallization of the desired 3C phase, resulting in a homogeneous phase and halide distribution. Consequently, blade-coated 1.

Global, regional, and national burden of multiple myeloma, 1990 to 2021 and predictions to 2035: an analysis of the Global Burden of Disease Study 2021.

Background And Objective: Multiple myeloma (MM) is the second most common haematologic malignancy. This study aimed to assess the global burden of multiple myeloma across 204 countries and territories from 1990 to 2021.

Methods: The data for this study were obtained from the Global Burden of Disease (GBD) 2021 dataset, which provides comprehensive information on the global and regional burden of 369 diseases, injuries, and 88 risk factors across 204 countries and territories from 1990 to 2021.

Controllable Configuration of Constitutional Units in Vanadium/Iron-Based Polyanionic Compounds for Sodium-Ion Storage.

Sodium-ion batteries (SIBs) are considered one of the most promising next-generation energy storage solutions. Among various electrode materials, vanadium/iron-based polyanionic compounds show significant potential as SIB cathodes due to their stable structures and high operating voltages, yet challenges persist in electron/Na transport and energy density. Although doping is an effective solution, the structure-activity relationship between dopant-induced configuration of octahedral/tetrahedral units and electrochemical performance remains poorly understood.

Green Extraction of Tea Polysaccharides Using Ultrasonic-Assisted Deep Eutectic Solvents and an Analysis of Their Physicochemical and Antioxidant Properties.

In this study, the ultrasonic-assisted extraction of deep eutectic solvents (UADES) for tea polysaccharides was optimized, and their physicochemical properties and antioxidant activities were analyzed. The optimal DES comprised choline chloride (CC) and ethylene glycol (EG) in a molar ratio of 1:3, with a water content of 40%. The optimized condition was an extraction temperature of 61 °C, an ultrasonic power of 480 W, and an extraction time of 60 min.

Application of a computer vision algorithm to quantify the frequency and duration of children's microactivities in different play scenarios.

Background: Microactivity rates, including hand- and object-to-mouth contacts, are inputs for modeling children's exposure to chemicals in soil and dust and from toys. EPA's confidence in its current recommended microactivity frequency estimates for use in exposure assessments is low.

Objective: We aimed to quantify children's hand- and object-to-mouth microactivities using a novel computer vision method and explored differences by lifestage and fine and gross motor development.

Blood plasma proteome-wide association study implicates new proteins in type 2 diabetes mellitus pathogenesis.

The pathophysiological mechanisms underlying type 2 diabetes mellitus (T2DM) remain incompletely understood, and the disease continues to impose a substantial burden on global health. In this study, we integrated the data from the largest genome-wide association study (GWAS; N = 898,130) of T2DM with human plasma protein quantitative trait locus (pQTL; N = 53,022) data to conduct the first proteome-wide association study (PWAS) of T2DM. Following Mendelian randomization and colocalization analyses, we identified nine independent putatively causal proteins.

Remarkable tumor response to Iruplinalkib in a 14-year-old girl with -positive advanced metastatic non-small-cell lung cancer: a case report.

Targeted therapies for anaplastic lymphoma kinase () mutations in non-small cell lung cancer (NSCLC) generally extend survival and alleviate symptoms. However, significant tumor reduction or complete remission remains rare. We report a rare case of a 14-year-old girl, whose father and grandfather both had lung cancer, diagnosed with advanced, multi-site metastatic -positive NSCLC.

Porous nanostructured cationic conjugated polyelectrolyte/titanium dioxide nanosheet heterojunction for enhanced cocatalyst-free photocatalytic hydrogen evolution.

Modified TiO photocatalysts face challenges dependence on cocatalysts, weak interfacial interactions, and poor wettability. This work presents an effective strategy by coupling donor-donor (D-D) type cationic conjugated polyelectrolyte poly[(9,9-bis(3'-((N,N-dimethyl)-N-ethylammonium)-propyl)-2,7-fluorene)-alt-2,7-(9,9-dioctylfluorene)]dibromide (PFN-Br) with two-dimensional TiO (2D-TiO₂) nanosheets to construct type-II heterojunctions rich in active sites. Under optimal conditions, the 2D-TiO/50 wt% PFN-Br (2D-Ti/PBr-50) heterojunction achieved a hydrogen evolution reaction of 2621.

Self-Toughened 2D Moiré Superlattice Membranes with Extreme Thermal Shock Tolerance.

Excellent mechanical strength and toughness are demanded for two-dimensional material (2DM) membranes in various applications to withstand extreme strain and temperature changes and resist crack propagation. However, the trade-off between strength and toughness poses significant challenges in meeting these requirements. This study presents a self-toughened 2D moiré superlattice membrane composed of vertically stacked hexagonal boron nitride and graphene (hBN/Gr) that exhibits high mechanical strength.

Comparison between cap-aspiration lumpectomy versus endoscopic submucosal dissection for the treatment of small gastric submucosal tumors: a prospective randomized controlled trial.

Background: Small (≤ 10 mm) gastric submucosal tumors (SMTs) are common clinical findings. Endoscopic resection has emerged as a promising alternative which enables early resection, but data comparing various methods remain limited. We aimed to compare the safety and efficacy of cap-aspiration lumpectomy (CASL) and endoscopic submucosal dissection (ESD) for resection of small gastric SMTs.

Exogenous arginine induced metabolic modulation and biomass enhancement in Nannochloropsis oceanica under high CO stress.

Given that excessive CO induces reactive oxygen species (ROS) which inhibit photosynthesis and microalgal survival, this study explores how exogenous arginine enhances stress resilience and biomass yield of Nannochloropsis oceanica under high CO stress. Arginine promoted NO synthesis, enhancing antioxidant enzyme activities to combat ROS damage. Transcriptomic analysis revealed that key enzymes in the Calvin cycle (PRK, GAPDH, FBPase and SBPase) and TCA cycle (CS, IDH, SCS and MDH) were significantly up-regulated, supporting carbon fixation and energy metabolism.

Blood plasma proteome-wide association study implicates novel proteins in the pathogenesis of multiple cardiovascular diseases.

Background: Cardiovascular diseases (CVD) are the leading cause of global mortality, yet current treatments benefit only a subset of patients. To identify new potential treatment targets, we conducted the first proteome wide association study (PWAS) for 26 CVDs using plasma proteomics data from the largest cohort to date (53,022 individuals in the UK Biobank Pharma Proteomics Project (UKB-PPP)).

Methods And Results: We calculated single nucleotide polymorphism (SNP)-protein weights using the UKB-PPP dataset and integrated these weights with genome-wide association study (GWAS) summary statistics for 26 CVDs across three categories (16 cardiac, 5 venous, and 5 cerebrovascular diseases) in up to 1,308,460 individuals.

Genetic Diversity and Construction of Salt-Tolerant Core Germplasm in Maize ( L.) Based on Phenotypic Traits and SNP Markers.

Maize is an essential staple food, and its genetic diversity plays a central role in breeding programs aimed at developing climate-adapted cultivars. Constructing a representative core germplasm set is necessary for the efficient conservation and utilization of maize genetic resources. In this study, we analyzed 588 cultivated maize accessions using agronomic traits such as plant morphology and yield traits such as ear characteristics and single-nucleotide polymorphisms (SNPs) to assess molecular diversity and population structure and to construct a core collection.

Phenological Stage and Nitrogen Input Coordinately Regulate Bud Bank Dynamics and Shoot Allocation in an Alpine Clonal Perennial Grass.

Belowground buds play a vital role in the clonal propagation and structural regulation of perennial herbaceous plants, especially in alpine environments, where vegetative renewal depends heavily on bud bank dynamics. However, the interactive effects of nitrogen addition and phenological stages on bud development and aboveground branching remain poorly understood. In this study, we examined the responses of rhizome buds, tiller buds, and aboveground tiller types of Kentucky bluegrass to six nitrogen levels (0, 6, 9, 12, 15, and 18 g/m) across five growth stages on the Qinghai-Tibet Plateau.

The Effect of Aging Treatment on the Properties of Cold-Rolled Cu-Ni-Si-Co Alloys with Different Mg Contents.

Cu-Ni-Si is a prominent example of a high-end lead frame copper alloy. The enhancement of strength without compromising electrical conductivity has emerged as a prominent research focus. The evolution of the precipitates exerts a significant influence on the strength and electrical conductivity of Cu-Ni-Si-Co-Mg alloys.

Improving age prediction using ECG signals: Insights into lifestyle impacts.

Background: The discrepancy between cardiac age, estimated from electrocardiograms (ECGs), and chronological age can serve as an indicator of cardiovascular health. A significant difference may reflect vascular aging and elevated risk, but improving the precision and interpretability of these predictions remains challenging.

Methods: This study proposes novel preprocessing techniques to improve prediction accuracy and investigates the relationship between lifestyle factors and cardiac aging.

Multiomics Reveals Nonphagocytosable Microplastics Induce Colon Inflammatory Injury via Bile Acid-Gut Microbiota Interactions and Barrier Dysfunction.

Microplastics (MPs), as emerging global environmental pollutants, exhibit intestinal toxicity mechanisms that are closely associated with the particle size. Nonphagocytosable MPs (NPMs), though incapable of being internalized by intestinal epithelial cells, still provoke colonic inflammatory damage. However, the exact mechanisms remain elusive.

Luminescent Cyclometalated Iridium(III) Polypyridine Complexes Modified with a Dendritic Guanidinium Moiety as Molecular Glues for Combined Chemo-Photodynamic Therapy.

We report herein three cyclometalated iridium(III) polypyridine complexes appended with a dendritic guanidinium unit [Ir(N^C)(bpy-Gu)](Cl) (bpy-Gu = 4-(-(3,4,5-tris(2-(2-(2-(4-((3,4,5-tris(2-(2-(2-guanidinoethoxy)ethoxy)ethoxy)benz-amido)methyl)-1-1,2,3-triazol-1-yl)ethoxy)ethoxy)ethoxy)phenylcarbonyl)aminomethyl)-4'-methyl-2,2'-bipyridine; HN^C = 2-phenylpyridine (Hppy) (), 2-phenylquinoline (Hpq) (), and 2-(1-naphthyl)benzothiazole (Hbsn) ()) as molecular glues. Their guanidinium-free counterparts [Ir(N^C)(bpy-C4)](Cl) (bpy-C4 = 4-(-(-propylcarbonyl)aminomethyl)-4'-methyl-2,2'-bipyridine; HN^C = Hppy (), Hpq (), and Hbsn ()) were also isolated. Irradiation of the complexes led to intense greenish-yellow to red emission.

Plasma Metabolomics and Machine Learning Reveals Metabolic Alterations and Diagnostic Biomarkers for Deep Venous Thrombosis in Hypertensive Patients after Traumatic Fracture.

We aimed to explore the metabolic dysregulations and diagnostic biomarkers for post-traumatic deep venous thrombosis (pt-DVT) in hypertensive (HPT) patients after fracture. An untargeted ultraperformance liquid chromatography-mass spectrometry-based metabolomics approach was employed to perform a comprehensive metabolic analysis of plasma samples of 80 patients with post-traumatic deep venous thrombosis and hypertension (pt-DVT&HPT) and 117 patients with hypertension only (HPT). Thirty-seven (37) differential metabolites were identified between pt-DVT&HPT and HPT patients.