Homozygous variant in JARID2 causes female infertility characterized by compromised blastulation efficiency.

Background: Impaired embryonic developmental competence is a critical determinant of assisted reproductive technology (ART) failure, yet current genetic diagnostics primarily address complete early embryonic arrest, leaving partial developmental defects unexplained, such as compromised blastulation efficiency (CBE). Jumonji and AT-rich interaction domain containing 2 (JARID2), encoding a chromatin-modifying factor essential for histone methylation regulation, emerges as a novel candidate in this context.

Results: We identified a homozygous JARID2 missense variant (c.

A novel PADI6 splice-site variant induces non-canonical GC-AG splicing and embryonic arrest in humans.

Early embryonic arrest (EEA) represents the predominant cause of assisted reproductive technology (ART) failure. PADI6 was a disease-associated gene identified for EEA. To date, 42 likely causal PADI6 variants have been documented, predominantly located in exonic regions with clustering outside the N-terminal domain.

Biallelic variants in DLGAP5 cause spindle assembly defects and human early embryonic arrest.

Study Question: What effects do DLGAP5 defects have on human early embryo development?

Summary Answer: DLGAP5 deficiency disrupts normal spindle assembly through its interaction with TACC3, leading to female infertility characterized by recurrent early embryonic arrest (REEA).

What Is Known Already: REEA is a significant contributor to failures in assisted reproductive technology. While genetic factors play a crucial role, known gene variants account for only a small proportion of affected individuals, leaving many underlying genetic factors yet to be elucidated.

Profiling learning styles and strategies of nursing students: A cluster analysis study.

Background: A learner profile, as a method for presenting students' core characteristics and behaviors, has been widely recognized in personalized education, yet it remains underexplored in the context of diversified nursing teaching modes. Nursing requires the application of theoretical knowledge of the curriculum to clinical practice procedures, demanding that nursing students not only absorb information but also transform, integrate, and critically apply it in dynamic, real-world settings. Investigating nursing students' learning style preferences and behavioral strategies is critical for understanding how to adapt teaching methods to meet varied learning needs, thereby enhancing teaching quality and improving student learning outcomes.

Social Capital and Job Performance: A Moderated Mediation Model of Organizational Citizenship Behaviors and Psychological Capital.

Taiwan's high-tech industry is experiencing rapid labor restructuring driven by automation and technological advancement, resulting in increased job demands and workplace stress. In this context, identifying mechanisms that sustain employee performance has become a critical concern. Drawing on the concept of social capital and grounded in Conservation of Resources (COR) theory, this study investigates whether social capital indirectly enhances job performance through organizational citizenship behavior.

Digital exclusion in older adults: A scoping review.

Background: The intersection of aging and digitalization is a distinctive feature of this new era. While digitalization brings convenience, it also forces older people to use tools that did not exist during their formative or working years. Understanding digital exclusion in older adults, the primary demographic affected, is essential to better support integration into the digital society, particularly in enhancing digital healthcare services' usability, continuity, and precision.

Cloning of the DlERF10 gene from Diospyros lotus L. and cold tolerance analysis of the DlERF10 gene in transgenic tobacco plants.

In the north of China, Diospyros plants are vulnerable to low-temperature damage in winter and is considered as a major factor restricting the development of the persimmon industry in Northern China. Diospyros lotus L. is featured by high survival potential of seedlings, cold tolerance, and grafting affinity with D.

Prognostic models for unplanned intensive care unit readmission risk prediction: A systematic review and meta-analysis based on HSROC model.

Background: Intensive care unit (ICU) readmission is a critical factor in determining discharge timing and transitional care and is predicted by various models using different approaches. A systematic review is needed to assess the performance and applicability of these models.

Aim: To identify prognostic models for unplanned ICU readmission and compare the performance of machine learning models with scoring systems.

Stellate ganglia block reduces airway hyperresponsiveness with modulates the IKK/NF-κB/IL-4/IL-5/IL-13 pathway.

Background: Airway hyperresponsiveness (AHR) is characterized by excessive contraction of airway smooth muscle, leading to airflow limitation, increasing perioperative airway spasm and even triggering the defense of silent lungs, which can lead to delayed surgery. Stellate ganglion blockade (SGB) has gained attention for its immunomodulatory and anti-inflammatory effects; however, its impact on AHR and the underlying mechanisms remain unexplored. This study aims to evaluate whether SGB reduces AHR and if this effect is related to inflammation.

Exploring the impact of social influence on parents' intention to use institutional childcare: a cross-sectional study.

Background: China is actively responding to low birth rates by developing childcare institutions to alleviate the parenting pressures on families. However, despite strong childcare demand, the enrollment rate remains low. Few studies have explored the role of social influence in parents' childcare decisions.

Psychometric Properties of the Chinese Version of the Arthritis Self-Care Behaviors Scale.

Background: In Chinese intervention studies, the lack of specific self-care scales based on the functional characteristics of Rheumatoid arthritis (RA) patients has caused patients and researchers to spend a great deal of time completing multiple related scales during the research work. Therefore, the arthritis Self-Care Behaviors Scale (SCBS) was developed to evaluate the self-care behavior of patients with arthritis.

Objective: The objectives of this study were to translate the SCBS into Chinese and test its psychometric properties in Chinese patients with RA.

Self-Management Behaviours in Rheumatoid Arthritis Patients: What Role Do Health Beliefs Play?

Purpose: Self-management is the intrinsically controlled ability of an active, responsible, informed and autonomous individual to live with the medical, role and emotional consequences of his chronic conditions in partnership with his social network and the healthcare providers. This study evaluated the self-management behaviours of patients with rheumatoid arthritis and assess the association between health beliefs and self-management behaviours.

Methods: This cross-sectional study investigated 269 rheumatoid arthritis patients' self-management behaviours using the Self-Care Behaviours Scale with a score of 0-4 for each item and a total score of 0-100 points, and health beliefs using the Arthritis Health Belief Inventory with a score of 1-5 for each item and a total score of 0-165 points.

Rapid-release and user-friendly costunolide/dehydrocostuslactone hydrophilic nanofibers: Therapeutic effects on acute gastric ulcers.

Gastric ulcers often cause postprandial epigastric pain, especially in acute cases. Abnormal motility, with about 50 % of patients having delayed gastric emptying, contributes to ulcer development. Costunolide (COS) and dehydrocostuslactone (DEH), derived from "Mu xiang" herbs, show potential in treating ulcers and regulating gastrointestinal motility.

Five racemic phthalides from the aerial parts of Lycopodiastrum casuarinoides and their neuroprotective activities.

Five racemic phthalides (1-5), including four undescribed phthalides monomers [(+)-1, (+)-2, (-)-2 and (-)-3], four undescribed phthalide dimers [(+)-4, (-)-4, (+)-5 and (-)-5], together with two known compounds [(-)-1 and (+)-3], were isolated from the aerial parts of Lycopodistrum casuarinoides. Their chemical structures were delineated by extensive spectroscopic data (UV, 1D/2D NMR, HRESIMS), in combination with the comparison of the experimental and calculated electronic circular dichroism spectra, calculated spin-spin coupling constants, and calculated NMR. All compounds were reported from Lycopodiaceae family for the first time.

Fast Seawater Desalination Integrated with Electrochemical CO Reduction.

Coupling desalination with electrocatalytic reactions is an emerging approach to simultaneously addressing freshwater scarcity and greenhouse gas emissions. However, the salt removal rate in such processes is slow, and the applicable water sources are often limited to those with high salt concentrations. Herein, we show high-performance electrocatalytic desalination by coupling with electrochemical CO reduction using a carbon catalyst.

The effects of music therapy on patients with chronic obstructive pulmonary disease: A systematic review and meta-analysis.

Background: Inconsistent findings concerning the effects of music therapy on patients with chronic obstructive pulmonary disease (COPD) have been reported.

Objective: To systematically evaluate the effects of music therapy on patients with COPD.

Methods: Database search was conducted in Cochrane Library, Web of Science, PubMed, Embase, CINAHL, CNKI, CBM, WanFang Data, and VIP databases, from database inception to June 2024.

Hyaluronic acid dissolving microneedle patch-assisted acupoint transdermal delivery of triptolide for effective rheumatoid arthritis treatment.

Triptolide (TP), a major active component of the herb Tripterygium wilfordii Hook F, has been shown excellent pharmacological effects on rheumatoid arthritis. However, TP is prone to causing severe organ toxicity, which limits its clinical application. In recent years, microneedle technology has provided a new option for the treatment of arthritis due to its advantages of efficient local transdermal drug delivery.

Local TSH/TSHR signaling promotes CD8 T cell exhaustion and immune evasion in colorectal carcinoma.

Background: Dysfunction of CD8 T cells in the tumor microenvironment (TME) contributes to tumor immune escape and immunotherapy tolerance. The effects of hormones such as leptin, steroid hormones, and glucocorticoids on T cell function have been reported previously. However, the mechanism underlying thyroid-stimulating hormone (TSH)/thyroid-stimulating hormone receptor (TSHR) signaling in CD8 T cell exhaustion and tumor immune evasion remain poorly understood.

Biallelic variants in α-tubulin isotypes cause female infertility characterised as recurrent preimplantation embryo arrest.

Background: Recurrent preimplantation embryo developmental arrest (RPEA) is the most common phenotype in assisted reproductive technology treatment failure associated with identified genetic abnormalities. Currently known maternal genetic variants explain only a limited number of cases. Variants of the β-tubulin subunit gene, , cause oocyte meiotic arrest and RPEA through a broad spectrum of spindle defects.

Role of hsa_circ_0007482 in pterygium development: insights into proliferation, apoptosis, and clinical correlations.

Aim: To investigate the impact of hsa_circ_0007482 on the proliferation and apoptosis of human pterygium fibroblasts (HPFs) and its correlation with the severity grades of pterygium.

Methods: Pterygium and normal conjunctival tissues were collected from the superior area of the same patient's eye (=33). The correlation between pterygium severity and hsa_circ_0007482 expression using quantitative reverse-transcription polymerase chain reaction (RT-qPCR) were analyzed.

Relative peripheral refraction in school children with different refractive errors using a novel multispectral refraction topographer.

Aim: To compare relative peripheral refraction (RPR) in Chinese school children with different refractive errors using multispectral refraction topography (MRT).

Methods: A total of 713 eyes of primary school children [172 emmetropia (E), 429 low myopia (LM), 80 moderate myopia (MM), and 32 low hypermetropia (LH)] aged 10 to 13y were analyzed. RPRs were measured using MRT without mydriasis.

Constructing a User-Centric Visual Real-Time Surgical Dashboard.

The existing surgery timetable is very simple, not user-friendly, and has very limited information. This article outlines the creation of a surgical dynamic dashboard for users, using Microsoft Power BI. The dashboard was developed based on in-depth interviews with the surgical team members to determine their requirements.

A gel plaster in the form of nipple cover: A comfortable and safe transdermal delivery method for mammary hyperplasia.

Hyperplasia of mammary glands (HMG) is considered a precancerous condition with a risk of malignant transformation, highlighting the necessity of proactive treatment in the early stages. Transdermal drug delivery offers significant advantages such as painlessness, absence of first-pass effect, and good patient compliance. However, the unique structure of the breast requires transdermal formulations for treating mammary hyperplasia to exhibit higher levels of safety and comfort.