Enhanced mixing and mass transfer of non-Newtonian fluids for DHA production via Schizochytrium sp. fermentation.

Docosahexaenoic acid (DHA) production via aerobic fermentation of Schizochytrium sp. is a green strategy, and the fermentation broth is a highly viscous, non-Newtonian fluid. In this study, the mixing and mass transfer of non-Newtonian fluids were enhanced using computational fluid dynamics combined with particle image velocimetry.

[Immune function regulation and tumor-suppressive effects of Shenqi Erpi Granules on S_(180) tumor-bearing mice].

This study aims to establish the S_(180) tumor-bearing mice model, and to investigate the influence of Shenqi Erpi Granules(SQEPG) on immune function, as well as the drug's tumor-suppressive effect and mechanism. SPF grade KM mice(half male and half female) were randomly divided into 6 groups: a control group, a model group, a cyclophosphamide group(50 mg·kg~(-1)), as well as SQEPG groups in low-, medium-, and high-dose(5.25, 10.

Distribution of glucosinolates and antioxidants in different leaf-layers of Kale ( var. DC.).

. Kale ( var. DC.

Constructing Monolayer Fe Clusters as Model Catalysts for CO Electroreduction.

Constructing model catalysts to clarify the active sites and elucidate structure-activity relationships represents a bottleneck issue in electrocatalytic reactions. For instance, Fe-based materials have been widely investigated for various electrocatalytic reactions, e.g.

Inducing superior electrical performances in textured CaBiTaO based ceramics.

Bismuth-layered structure ferroelectrics (BLSFs), exemplified by CaBiTaO (CBTa), exhibit exceptional thermal stability at high temperatures with a high Curie temperature. This attribute renders them highly promising candidates for piezoelectric sensors, transducers, non-volatile ferroelectric memory, working in extreme environments. However, CBTa ceramic suffers from the following intrinsic limitations: spontaneous polarization confined within the -plane of the unit cell and a large coercive field, leading to severely suppressed piezoelectric activity ( ≈ 5.

Co-catalysis of modified metal-organic framework immobilized enzyme with nonionic surfactant for biodiesel preparation.

With the increasing demand for sustainable energy, enzyme-catalyzed biodiesel preparation has garnered significant interest owing to its green and efficient characteristics. However, conventional immobilized lipase (LIP) is susceptible to desorption and inactivation and fails to balance the catalytic efficiency and stability, limitations that seriously restrict the industrial application. To address this, we constructed a carrier through hydrophobically modifying University of Oslo-66-NH (UiO-66-NH), an amino-functionalized metal-organic framework (MOF), with octadecylphosphonic acid (OPA).

lncRNA EBLN3P Promotes Proliferation, Metastasis and Stemness of Gastric Cancer Cells via miR-141-3p/HMGCS1.

Long non-coding RNAs (lncRNAs) have become an essential factor in gastric cancer (GC) initiation and metastasis. This study is aimed to uncover the functional significance of EBLN3P in GC. In GC, EBLN3P and HMGCS1 was significantly increased.

Long-term survival of a SMARCA4-deficient undifferentiated thoracic tumor with brain metastasis successfully treated with multimodal treatment: a case report and literature review.

Background: SMARCA4-deficient undifferentiated thoracic tumor (SMARCA4-UT) is a rare and highly aggressive malignancy characterized by early distant metastasis and a poor prognosis, with a median overall survival (OS) of only 4-7 months. Traditional therapies offer limited benefit, while emerging data suggest the efficacy of combined immunotherapy, chemotherapy, and anti-angiogenic approaches. We report a case of a 52-year-old male with a heavy smoking history who presented with loss of consciousness and limb convulsions.

Global research landscape of retinoblastoma biomarkers: a multidisciplinary bibliometric analysis based on multiple databases (2005-2025).

Objective: Research exploring biomarkers for retinoblastoma (RB) diagnosis exists; however, their specific impact on RB has not been thoroughly investigated through systematic quantitative analysis. This study aims to analyze the research landscape and hotspots of RB biomarkers from 2005 to 2025, providing a theoretical reference for future investigations.

Methods: We retrieved publications from the Web of Science and Scopus databases published between 2005 and 2025, followed by analysis using R software, VOSviewer, and CiteSpace tools.

Using serum cTnT/CK combined with anti-Ro52 antibodies to screen for myocardial involvement and assess prognosis in idiopathic inflammatory myopathy.

Objective: Myocardial involvement in idiopathic inflammatory myopathy (IIM) indicates poor prognosis. This study aimed to identify risk factors for myocardial involvement and assess their prognostic value.

Methods: We analysed 92 IIM patients with abnormal cardiac troponin T (cTnT).

Interferon-α and thymosin-α1 plus tislelizumab enhance CD8 T cell cytotoxicity toward pancreatic ductal adenocarcinoma.

The strong immunosuppression and immune evasion of pancreatic ductal adenocarcinoma (PDAC) result in poor efficacy of immune checkpoint blockade. In this study, the PD-1 level on CD8 T cells in the peripheral blood of patients with PDAC was significantly greater than that in the peripheral blood of healthy individuals. To enhance the anticancer activity of adoptive CD8 T cells toward PDAC, interferon-α (IFN-α) and thymosin-α1 (Tα1) plus tislelizumab were preclinically explored.

D-glucaro-1,4-lactone alleviates acetaminophen-induced hepatotoxicity in mice via modulating gut microbiota and metabolites associated with -glutamine and nicotinic acid pathways.

Objective: This study investigated the hepatoprotective effect and underlying mechanisms of D-glucaro-1,4-lactone (1,4-GL), a natural compound found in fruits and vegetables, against acetaminophen (APAP)-induced acute liver injury (ALI) in mice, which had not been previously explored.

Methods: A stable ALI model was established in male C57BL/6J mice using 300 mg/kg APAP after fasting. Mice were pretreated orally with glutathione (200 mg/kg), or 1,4-GL (100 mg/kg or 200 mg/kg) for five consecutive days before APAP challenge.

Amorphization-Induced Electronic Modulation of Gd(OH) Nanocages with Enhanced Enzymatic Activities for Antitumor Therapy.

In nanotechnology-based cancer therapy, modulating electronic states of nanomaterials is crucial for influencing spatiotemporal dynamic behaviors of intracellular reduction-oxidation and redox homeostasis. Although rare-earth transition metals with 4f electrons present electronic energy levels suitable for electronic modulation, its practical realization is challenging due to strong 4f electron localization. Theoretical studies indicate that amorphization can significantly alter the electronic states of the 4f-dominated nanomaterials.

Isoform-specific function of NSD3 in DNA replication stress confers resistance to PARP inhibitors in prostate cancer.

Poly (ADP-ribose) polymerase (PARP) inhibitors (PARPi) have been approved for the treatment of metastatic castration-resistant prostate cancer (mCRPC) in patients with deleterious BRCA1/2 alterations. Although this marks a significant milestone, intrinsic or acquired therapy resistance remains a major challenge that limits clinical efficacy. Here, we demonstrate that dysregulated ubiquitination and turnover by the cullin 3 (CUL3) E3 ligase complex induce the upregulation of the short isoform of nuclear-receptor-binding SET domain protein 3 (NSD3) (NSD3S), which confers PARPi resistance in prostate cancer cells and patient-derived mCRPC samples.

Modulating the local microenvironment over isolated nickel sites through first-shell coordination to regulate the reaction pathway of CO electroreduction.

Manipulating the local microenvironments of single-atom catalysts is crucial for the product selectivity of CO electroreduction. Although theoretical research suggests that modifying the coordination structure of isolated Ni sites can promote the reduction of CO to CH, there is still no experimental evidence to date. Herein, by regulating the coordination shell of boron (B) surrounding the Ni central atom, we have achieved the transformation of the reduction product from CO to CH.

Biochemical and Functional Characterization of Aminopeptidase N: A New Role as a 6-Monoacetylmorphine Hydrolase.

6-monoacetylmorphine (6-MAM), a primary active metabolite of heroin that reaches the human brain, plays a crucial role in producing heroin-associated physiological and lethal effects. Therefore, 6-MAM has emerged as a key target for alleviating the adverse consequences of heroin abuse. In this study, the proposed 6-MAM hydrolase aminopeptidase N (eAPN) was recombinantly produced, and its biochemical and functional profiles were investigated.

Reducing neuroinflammation through PKCγ/p38/NF-κB signaling pathway: the mechanism of Danzhi Xiaoyaosan in improving post-stroke depression.

Ethnopharmacological Relevance: Post-stroke depression (PSD) is a common sequela of stroke, causing tremendous mental burden to patients. Danzhi Xiaoyao San (DZX) is a widely used traditional Chinese medicine formula for the clinical treatment of PSD and has favorable therapeutic effects. However, its mechanism of action in treating PSD is still unclear.

Revealing the Ferroelectric Fatigue Pathways in HfO₂ Film.

Hafnium oxide (HfO₂) has emerged as a transformative material for next-generation non-volatile memory technologies due to its unique ability to exhibit ferroelectricity in ultrathin films. Its practical application is critically hindered by polarization fatigue and depolarization phenomena, while the inherent complexity of these transitions between ferroelectric and paraelectric state in HfO₂ has posed significant challenges. Here, symmetry analysis and with first-principles calculations is leveraged to systematically explore all potential transition pathways from the ferroelectric oIII/oIV phases to the paraelectric mI/mII phases.

Assessing the potential impacts of California Senate Bill 27 (SB27) on the antimicrobial susceptibility of from raw meat.

Background: Antimicrobial use in food-animal production selects for antimicrobial-resistant that can be transmitted to humans via contaminated meat products. California Senate Bill 27 (SB27), which took effect on January 1, 2018, restricts the use of medically important antimicrobials in California food-animal production. Over time, SB27 could reduce the prevalence of antimicrobial-resistant on meat produced in California.

Exploring the neuroplasticity hypothesis in depression: The role of traditional Chinese herbal medicine.

Background: Depression is a mental disorder that seriously affects global health, and its pathogenesis involves multiple factors, including changes in neuroplasticity. The neuroplasticity hypothesis suggests that there may be functional and structural abnormalities in synaptic plasticity, neuronal generation and maturation, neural circuits and related signaling pathways in patients with depression. Traditional Chinese medicine (TCM), as a traditional treatment method, has attracted increasing attention in the field of neuropharmacology in recent years due to its high efficiency and low toxicity.

Highly Exposed Ultra-Small High-Entropy Sulfides with d-p Orbital Hybridization for Efficient Oxygen Evolution.

Precise regulation of electronic structure and nanoscale geometry represents a transformative strategy for breaking the activity-stability trade-off in oxygen evolution electrocatalysts. Here, highly exposed ultra-small high-entropy sulfides (HES, 5.2 nm) confined in porous carbon nanofibers are designed.

Detection of Different Patterns of Genome-Wide Gene Expression Disturbance in Three Nullisomy Lines in Allotetraploid .

Aneuploidy-related disruptions are generally tolerated in polyploid plants, which exhibit a greater capacity for genomic compensation. In this study, we utilize allotetraploid as a model and generated three aneuploid variants (NC1, NC2, and NC8) to investigate the phenotypic and transcriptional consequences of chromosome loss. Significant phenotypic variations were observed, with the most notable being a marked dwarfing phenotype in the aneuploid materials compared to the euploid Oro.

Development of cocaine esterase W/O/W nanoemulsions by a novel low-temperature double emulsification approach for cocaine abuse treatment.

Cocaine esterase is highly effective against cocaine but has a short in vivo half-life and is sensitive to temperature, limiting its clinical use. Encapsulation of cocaine esterase in water-in-oil-in-water (W/O/W) nanoemulsions offers a promising approach to improve its stability and therapeutic effectiveness. In this study, we developed a novel low-temperature double emulsification method to encapsulate the cocaine esterase mutant (E196-301) into W/O/W nanoemulsions (designated as CocE NEs).

Assembly of DNA Networks on the Cell Membrane Enables Confined Detection of MMP-9 Secretion and Evaluation of Drug Efficacy.

Proteases secreted by tumor cells have been employed as potential biomarkers for the early assessment of tumor metastasis risk. However, in situ monitoring of their secretion at the single-cell level remains a challenge due to their low abundance and diffusion into complex environments. Despite significant advancements in utilizing DNA as a versatile scaffold for constructing dynamic sensing systems due to its programmability and design flexibility, the spatial control of molecular sensing and imaging functions within living cells is particularly challenging.