Photothermally Driven Ultrafast Polymerase Chain Reaction: Mechanisms, Nanomaterial Architectures, and System Integration.

As one of the most important technologies in molecular biology, polymerase chain reaction (PCR) has been widely recognized in many fields such as infectious disease diagnosis due to its high sensitivity, specificity, and accuracy. Attempts in this field are focused on developing efficient heating mechanism to achieve efficient thermal cycles. Recently, with the in-depth research into photothermal effects, photonic PCR technology based on photothermal nanomaterials has gradually demonstrated potential to develop a new generation of ultrafast PCR instrument.

Clinical application of flexible and variable transparent endport technology in minimally invasive neuroendoscopic keyhole surgery: a practical new technology.

With the development of endoscopic technology, neuroendoscopy utilizes its advantages such as good illumination, close range observation, and flexible degrees of freedom. Neuroendoscopic surgery can achieve advantages such as minimally invasive, high clearance rate, low incidence of complications, good brain tissue protection, and fewer surgery-related injuries. However, minimally invasive endoscopic surgery also has inherent limitations, since its narrow surgical channels are prone to collapse and require special instrument support.

A cleavage-gated terminal exposure-driven CRISPR-RCA self-amplifying system for ultra-fast DNA detection.

The one-pot detection technology based on nucleic acid isothermal amplification combined with CRISPR has a significant advantage in on-site detection of infectious diseases. It is superior to quantitative polymerase chain reaction (qPCR) due to its lack of temperature variation and significantly faster reaction speed. Nevertheless, Cas proteins compete with amplification enzymes for reaction substrates, which results in the signal amplification effect being less than expected.

Epicutaneous Staphylococcus aureus initiates cross-tissue IL-36R signaling for neutrophilic lung inflammation in a model of the atopic march.

Patients with atopic dermatitis exhibit abundant Staphylococcus aureus skin colonization and an increased risk of atopic march diseases, including allergic rhinitis, food allergies, and asthma. We have previously shown that S. aureus skin exposure exacerbates allergic lung inflammation in an interleukin-36 receptor (IL-36R)-dependent manner.

Sleep and Sugar: Deciphering the 2003-2023 Research Landscape on Sleep Disorders and Diabetes Mellitus via Bibliometric Study.

Objective: This study aims to provide a bibliometric analysis of the research landscape on sleep disorders and diabetes mellitus, exploring the volume and impact of scholarly publications, identifying leading contributors, and discerning emerging trends and hotspots within this field.

Methods: Utilizing the Web of Science™ Core Collection, we conducted a detailed search and analysis of publications related to sleep disorders and diabetes from January 2003 to December 2023. Tools like CiteSpace and VOSviewer were employed for data visualization, highlighting the contributions of authors, institutions, countries, and journals.

Abnormal β-Hydroxybutyrylation Modification of ARG1 Drives Reprogramming of Arginine Metabolism to Promote the Progression of Colorectal Cancer.

The abnormal arginine metabolism is characteristic of tumor cell metabolism in colorectal cancer (CRC). However, the mechanisms underlying arginine metabolic reprogramming and how altered metabolism in turn enhances CRC tumorigenicity are poorly understood. Protein post-translational modifications (PTMs) are crucial for regulating protein function, activity, and interactions.

Triple sheath neuroendoscopic combination technique for managing complete intraventricular hemorrhage casting in patients with cerebral hemorrhage.

Objective: To evaluate the efficacy of the triple sheath neuroendoscopic combination technique (TSNCT) compared to standard endoscopic hematoma removal and external ventricular drainage (EVD) for managing complete intraventricular hemorrhage (IVH) casting in patients with cerebral hemorrhage.

Methods: A retrospective analysis was conducted on five patients with complete IVH casting treated at our institution between 2023 and 2024, including two treated with TSNCT, two with standard endoscopic hematoma removal, and one with EVD. Preoperative and postoperative imaging, intraoperative neuroendoscopic video, and clinical data were reviewed.

Proteomic Profiling of Serum-Derived Extracellular Vesicles in Diffuse Idiopathic Skeletal Hyperostosis Patients.

This study focuses on the proteomic profiles of serum-derived EVs in diffuse idiopathic skeletal hyperostosis (DISH), aiming to identify the potential diagnostic biomarkers for DISH. Serum-EVs were separated using a differential centrifugation technique. Then, label-free-liquid chromatography mass spectrometry (LC-MS/MS) was utilized to identify differentially expressed proteins (DEPs) highly associated with DISH.

Identification of serum exosomal miRNA biomarkers in patients with lupus nephritis.

Introduction: Characterized by glomerulonephritis, lupus nephritis (LN) worsens the prognosis of patients with systemic lupus erythematosus (SLE) and contributes to its increased mortality rate. Here, we investigated whether serum exosomal microRNAs (miRNAs) are promising new biomarkers of LN.

Methods: Serum exosomes were initially isolated using a reagent-based kit, and total RNA was extracted with the Trizol method.

Ubiquitin Specific Protease 9X Regulates the Activation of ARK5 and Promotes Progression of Fibrotic Remodeling.

USP9X plays a crucial role in myocardial fibrosis. This study showed increased USP9X expression in myocardial infarction models, associated with collagen deposition and myofibroblast activation. Myofibroblast-specific USP9X knockout and pharmacologic inhibition with Degrasyn both reduced fibrosis and improved cardiac function.

Visible-light-switched radical/non-radical bichannel degradation mechanism via round-the-clock synergistic CDs-BiOCl/PS system.

In order to achieve efficient degradation of organic pollutants (OPs) and to avoid interference from co-existing anions and natural organic matter, a synergistic CDs-BiOCl/PS system is designed, which is capable of functioning under both dark and visible-light-controlled conditions, enabling round-the-clock operation. A novel nano photocatalyst CDs-BiOCl was constructed by modifying the surface oxygen vacancy (O) rich bismuth oxychloride (BiOCl) with carbon quantum dots (CDs). The key role of the edge carbon atoms (C) of CDs in CDs-BiOCl photocatalyst for PS (persulfate) activation was revealed.

Rilmenidine attenuates intracerebral hemorrhage by regulating autophagy flux and apoptosis via the HO-1/Fe/ERK pathway.

Intracerebral hemorrhage (ICH) associated with high mortality and disability rates. The limited understanding of the pathological mechanism of ICH has greatly limited the available treatment options. Rilmenidine (RIL) is a safe and effective imidazoline-1 receptor agonist that has been confirmed by the FDA to have good blood pressure-reducing effects.

Emerging themes and future directions in space radiation health research: a bibliometric exploration from 2013 to 2022.

The impact of space radiation on health (SRHE) is extensive and significantly influences public health and space operations, making it essential to analyze global collaboration networks and track developmental trends over the last decade. However, bibliometric analysis in this area remains limited. This study aims to outline publication trends, citation patterns, major journals, key authors, institutional and national collaborations, and to explore emerging themes and future directions.

Programmable production of bioactive extracellular vesicles in vivo to treat myocardial infarction.

Current myocardial infarction (MI) treatment strategies remain challenged in suboptimal pharmacokinetics and potential adverse effects. Here we present a bioelectronic interface capable of producing on-demand abundant bioactive extracellular vesicles (EVs) near the MI area for in-situ localized treatment. The technology, termed electroactive patch for wirelessly and controllable EV generation (ePOWER), leverages wireless bioelectronic patch to stimulate embedded electrosensitive macrophages, actively modulating the biosynthesis of EVs and enabling EV production with high programmability to be delivered directly to the MI area.

Accuracy of Large Language Models for Literature Screening in Thoracic Surgery: Diagnostic Study.

Background: Systematic reviews and meta-analyses rely on labor-intensive literature screening. While machine learning offers potential automation, its accuracy remains suboptimal. This raises the question of whether emerging large language models (LLMs) can provide a more accurate and efficient approach.

Bacterial cellulose-based Pickering emulsions reinforced with silver and silica nanoparticles for advanced antibacterial and hydrophobic food packaging solutions.

Conventional packaging materials typically exhibit insufficient barrier performance and limited antimicrobial efficacy, constraining their utility in food preservation. Enhancing attributes such as moisture resistance, antimicrobial potency, and stability in biodegradable matrices has presented a longstanding challenge. Herein, we report the design of an ODSA-based Pickering emulsion coating of bacterial cellulose, further modified with AgNPs and SiO (TAS), engineered to elevate food preservation standards within packaging applications.

Surface Components and Biological Interactions of Extracellular Vesicles.

Extracellular vesicles (EVs) are critical mediators of intercellular communication, carrying bioactive cargo and displaying diverse surface components that reflect their cellular origins and functions. The EV surface, composed of proteins, lipids, and glycocalyx elements, plays a pivotal role in targeting recipient cells, mediating biological interactions, and enabling selective cargo delivery. This review comprehensively examined the molecular architecture of EV surfaces, linking their biogenesis to functional diversity, and highlights their therapeutic and diagnostic potential in diseases such as cancer and cardiovascular disorders.

Planetary health diet index and mortality among US cancer survivors: mediating roles of systemic immune-inflammation index and neutrophil-to-lymphocyte ratio.

Background: Cancer-related deaths and environmental issues pose significant global challenges. The Planetary Health Diet (PHD) is a healthy dietary pattern that simultaneously promotes human health and ecology. This study aims to investigate the association between the Planetary Health Diet Index (PHDI) and mortality among cancer survivors, as well as the mediating role of inflammation between PHDI and all-cause mortality.

Research trends on lymphedema after mastectomy for breast cancer patients from 2000 to 2023: a scientometric analysis.

Background: Breast cancer-related lymphedema (BCRL) is a common and debilitating complication following breast cancer treatment. Despite its significant impact on patients' quality of life, bibliometric analyses focusing on BCRL are scarce. This study aims to explore global research trends on BCRL from 2000 to 2023, identify existing knowledge gaps, and highlight emerging focus areas through a bibliometric approach.

Colorimetric aptasensor coupled with a deep-learning-powered smartphone app for programmed death ligand-1 expressing extracellular vesicles.

Lung cancer is a devastating public health threat and a leading cause of cancer-related deaths. Therefore, it is imperative to develop sophisticated techniques for the non-invasive detection of lung cancer. Extracellular vesicles expressing programmed death ligand-1 (PD-L1) markers (PD-L1@EVs) in the blood are reported to be indicative of lung cancer and response to immunotherapy.

An ultrasensitive one-pot Cas13a-based microfluidic assay for rapid multiplexed detection of microRNAs.

Aberrant microRNA expression is associated with tumor progression in various organs. Detecting microRNAs as clinical cancer biomarkers can facilitate early cancer diagnosis and monitoring. However, the rapid and accurate quantification of microRNAs from biological samples remains a significant challenge.

Selective In Situ Analysis of Hepatogenic Exosomal microRNAs via Virus-Mimicking Multifunctional Magnetic Vesicles.

Drug-induced liver injury (DILI) is a common clinical problem with urgent respect to demanding early diagnosis. Exosomal miRNAs are reliable and noninvasive biomarkers for the early diagnosis of DILI. However, accurate and feasible detection of exosomal miRNAs is often hampered by the low abundance of miRNAs, inefficient exosome separation techniques, and the requirement for RNA extraction from large sample volumes.

Clinical application of 3D slicer reconstruction and 3D printing localization combined with neuroendoscopy technology in VPS surgery.

To explore techniques, advantages and disadvantages of 3D Slicer reconstruction and 3D printing localization technology combined with transcranial neuroendoscopy in ventriculoperitoneal shunt surgery. Retrospective analysis of clinical data of patients with hydrocephalus treated by ventriculoperitoneal shunt surgery using 3D Slicer reconstruction and 3D printing positioning technology combined with transcranial neuroendoscopy in our hospital from October 2021 to March 2023. A total of 33 patients with complete data were collected, including 19 males and 14 females, aged 10-81 years.

Machine learning-assisted washing-free detection of extracellular vesicles by target recycling amplification based fluorescent aptasensor for accurate diagnosis of gastric cancer.

Extracellular vesicles (EVs) are promising non-invasive biomarkers for cancer diagnosis. EVs proteins play a critical role in tumor progress and metastasis. However, accurately and reliably diagnosing cancers is greatly limited by single protein marker on EVs.

Rbfox3 Promotes Transformation of MDSC-Like Tumor Cells to Shape Immunosuppressive Microenvironment.

Myeloid-derived suppressor cells (MDSCs) within the tumor microenvironment (TME) contribute to the malignant progression of tumors by exerting immunosuppressive effects. Bacterial lipopolysaccharides (LPS) have been widely demonstrated in various types of solid tumors. LPS can promote the malignant progression of tumors, which mechanism has not yet been fully elucidated.